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1
Mechanism of calcium disilicide‐induced calcification of crystalline silicon surfaces in simulated body fluid under zero bias
Dergi Makalesi

Dergi Makalesi
Kaynak :
JSTOR
Dergi:
Journal of Biomedical Materials Research Part A
Yayıncı:
John Wiley & Sons, Inc.
Yazar(lar) :
Vladimir V. Seregin , Jeffery L. Coffer
Journal of Biomedical Materials Research Part A
Yayıncı Web Sitesi
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Kaydet
DOI : 10.1002/jbm.a.31691
2
Contact guidance enhances the quality of a tissue engineered corneal stroma
Dergi Makalesi

Dergi Makalesi
Open Access
Kaynak :
DSpace Makaleler
Dergi:
Journal of Biomedical Materials Research - Part A
Yayıncı:
John Wiley & Sons, Inc.
Yazar(lar) :
Bilkent Üniversitesi
Konu :
Collagen, Cornea, Micropatterning, Tissue engineering, Transparency, Artificial organs, Cells, Collagen, Transparency, Cornea, Human corneal keratocytes, Micropatterning, Tissue engineering, Collagen type 1, Keratan sulfate, Cell proliferation, Controlled study, Cornea, Cornea cell, Cornea stroma, Cross linking, Human cell, Scanning electron microscope, Tissue culture, Tissue engineering, Ultraviolet spectrophotometry, Adult, Animals
Yıl :
2008
Journal of Biomedical Materials Research - Part A
Yayıncı Web Sitesi
Tam Metin
Kaydet
Corneal stroma is a very complex structure, composed of 200 lamellae of oriented collagen fibers. This highly complex nature of cornea is known to be important for its transparency and mechanical integrity. Thus, an artificial cornea design has to take into account this complex structure. In this study, behavior of human corneal keratocytes on collagen films patterned with parallel channels was investigated. Keratocytes proliferated well on films and reached confluency after 7 days in the incubation medium. Nearly all of the cells responded to the patterns and were aligned in contrast to the cells on unpatterned surfaces. Collagen type I and keratan sulfate secreted by keratocytes on patterned films appeared to be aligned in the direction of the patterns. The films showed an intermediate degradation over the course of a month. On the whole, transparency of the films increased with degradation and decreased by the presence of the cells. The decrease was, however, low and transparency level was maintained on the patterned films while on the unpatterned films a sharp decrease in transparency was followed by an improvement. This was due to the more organized distribution of cells and the oriented secretion of extracellular matrix molecules on patterned collagen films. Thus, these results suggest that application of contact guidance in cornea tissue engineering may facilitate the remodeling process, hence decrease the rehabilitation period.
DOI : 10.1002/jbm.a.31442
Anahtar Kelimeler : Collagen, Cornea, Micropatterning, Tissue engineering, Transparency, Artificial organs, Cells, Collagen, Transparency, Cornea, Human corneal keratocytes, Micropatterning, Tissue engineering, Collagen type 1, Keratan sulfate, Cell proliferation, Controlled study, Cornea, Cornea cell, Cornea stroma, Cross linking, Human cell, Scanning electron microscope, Tissue culture, Tissue engineering, Ultraviolet spectrophotometry, Adult, Animals, Article
Cilt: 84
Sayı: 2
ISSN: 1549-3296
Sayfa: 454-463
Sınıflandırma :
3
In vitro chondrogenesis by BMP6 gene therapy
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Dergi Makalesi
Kaynak 1:
DSpace Makaleler
Kaynak 2:
DSpace Makaleler
Dergi:
Journal of Biomedical Materials Research - Part A
Yayıncı:
Zonguldak Bülent Ecevit Üniversitesi
Yazar(lar) :
Zonguldak Bülent Ecevit Üniversitesi
Konu :
BMP6, cartilage tissue engineering, chitosan scaffold, gene therapy, mesenchymal stem cell
Yıl :
2013
Journal of Biomedical Materials Research - Part A
Piri Tam Metin Bulucu
Kaydet
In this study, the promotion of in vitro chondrogenesis was investigated by using chitosan scaffolds and rat bone marrow-derived mesenchymal stem cells (rBMSCs) which are transfected by BMP6 (bone morphogenetic protein-6) encoding gene. For this purpose, plasmid DNA (pShuttle-rBMP6), the expression vector consisting of the coding sequence of the BMP6 was obtained, and then, it was entrapped in chitosan scaffolds to obtain a gene-activated matrix (GAM). The chitosan scaffolds performed the controlled and sustained release of plasmid DNA, thus they continuously provided the modification of rBMSCs to induce chondrogenic differentiation. In addition, the cells were transfected by lipid-based agent (Lipofectamine) and then, these modified cells were inoculated into the chitosan scaffolds. Furthermore, a group of chitosan scaffolds with nontransfected rBMSCs with recombinant BMP6 free in culture medium was used as control. Comparative results showed that, mitochondrial activities of modified rBMSCs by Lipofectamine and chitosan GAM were significantly higher than those of nontransfected rBMSCs. The observations from scanning electron microscopy analysis confirmed that BMP6 gene-modified rBMSCs differentiated to the chondrogenic phenotype. Highest amount of glycosaminoglycan contents of rBMSCs on GAM concluded that BMP6 gene-activated chitosan scaffold has a potential in the application of cartilage regeneration. © 2012 Wiley Periodicals, Inc.
DOI : 10.1002/jbm.a.34430
Anahtar Kelimeler : BMP6, cartilage tissue engineering, chitosan scaffold, gene therapy, mesenchymal stem cell, article
Cilt: 101 A
Sayı: 5
ISSN: 1549-3296
Sınıflandırma :
4
Tissue responses to novel tissue engineering biodegradable cryogel scaffolds: An animal model
Dergi Makalesi

Dergi Makalesi
Kaynak :
DSpace Makaleler
Dergi:
Journal Of Biomedical Materials Research Part A
Yazar(lar) :
Nimet Bolgen , Ibrahim Vargel , Petek Korkusuz , Elif Guzel , Fatima Plieva , Igor Galaev , Erhan Piskin
Konu :
tissue-engineering scaffolds, cryogels, biodegradable, HEMA-L-lactide-dextran, animal model, tissue reponses
Yıl :
2009
Journal Of Biomedical Materials Research Part A
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Kaydet
Biodegradable macroporous cryogels with highly open and interconnected pore structures were produced from dextran modified with oligo L-lactide bearing hydroxyethylmethacrylate (HEMA) end groups in moderately frozen solutions. Tissue responses to these novel scaffolds were evaluated in rats after dorsal subcutaneous implantation, iliac submuscular implantation, auricular implantation, or in calvarial defect model. In no case, either necrosis or foreign body reaction was observed during histological studies. The cryogel scaffolds integrated with the surrounding tissue and the formation of a new tissue were accompanied with significant ingrowth of connective tissue cells and new blood vessels into the cryogel. The tissue responses were significantly lower in auricular and calvarial implantations when compared with the subcutanous and the submuscular implantations. The degradation of the scaffold was slower in bone comparing to soft tissues. The biodegradable cryogels are highly biocompatible and combine extraordinary properties including having soft and elastic nature, open porous structure, and very rapid and controllable swelling. Therefore, the cryogels could be promising candidates for further clinical applications in tissue regeneration. (C) 2008 Wiley Periodicals, Inc. J Biomed Mater Res 91 A: 60-68, 2009
DOI : 10.1002/jbm.a.32193
Anahtar Kelimeler : tissue-engineering scaffolds, cryogels, biodegradable, HEMA-L-lactide-dextran, animal model, tissue reponses, article
Cilt: 91A
Sayı: 1
ISSN: 1549-3296
Sınıflandırma :
5
Salinomycin encapsulated nanoparticles as a targeting vehicle for glioblastoma cells
Dergi Makalesi

Dergi Makalesi
Kaynak :
DSpace Makaleler
Dergi:
Journal of Biomedical Materials Research - Part A
Yayıncı:
John Wiley and Sons Inc.
Yazar(lar) :
Bülent Ecevit Üniversitesi
Konu :
glioblastoma, nanoparticle, PLGA, polysorbate 80, salinomycin, targeting
Yıl :
2016
Journal of Biomedical Materials Research - Part A
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 Piri Tam Metin Bulucu
Kaydet
Salinomycin has been introduced as a novel alternative to traditional anti-cancer drugs. The aim of this study was to test a strategy designed to deliver salinomycin to glioblastoma cells in vitro. Salinomycin-encapsulated polysorbate 80-coated poly(lactic-co-glycolic acid) nanoparticles (P80-SAL-PLGA) were prepared and characterized with respect to particle size, morphology, thermal properties, drug encapsulation efficiency and controlled salinomycin-release behaviour. The in vitro cellular uptake of P80-SAL-PLGA (5 and 10 µM) or uncoated nanoparticles was assessed in T98G human glioblastoma cells, and the cell viability was investigated with respect to anti-growth activities. SAL, which was successfully transported to T98G glioblastoma cells via P80 coated nanoparticles (~14% within 60 min), greatly decreased (p < 0.01) the cellular viability of T98G cells. Substantial morphological changes were observed in the T98G cells with damaged actin cytoskeleton. Thus, P80-SAL-PLGA nanoparticles induced cell death, suggesting a potential therapeutic role for this salinomycin delivery system in the treatment of human glioblastoma. © 2015 Wiley Periodicals, Inc.
DOI : 10.1002/jbm.a.35591
Anahtar Kelimeler : glioblastoma, nanoparticle, PLGA, polysorbate 80, salinomycin, targeting, article
Cilt: 104
Sayı: 2
ISSN: 1549-3296
6
Incorporation of growth factor loaded microspheres into polymeric electrospun nanofibers for tissue engineering applications
Dergi Makalesi

Dergi Makalesi
Kaynak :
DSpace Makaleler
Dergi:
Journal of Biomedical Materials Research - Part A
Yayıncı:
Journal of Biomedical Materials Research - Part A
Yazar(lar) :
P. Selcan Gungor-Ozkerim , T. Balkan , G.T. Kose , A. Sezai Sarac , F.N. Kok
Konu :
biodegradable scaffold, controlled growth factor release, electrospun nanofibers
Yıl :
2014
Journal of Biomedical Materials Research - Part A
Yayıncı Web Sitesi
Kaydet
Nanofibrous double-layer matrices were prepared by electrospinning technique with the bottom layer formed from PCL (poly-?-caprolactone)/PLLA (poly-l-lactic acid) nanofibers and the upper layer from PCL/Gelatin nanofibers. Bottom layer was designed to give mechanical strength to the system, whereas upper layer containing gelatin was optimized to improve the cell adhesion. Gelatin microspheres were incorporated in the middle of two layers for controlled growth factor delivery. Successful fabrication of the blend nanofibers were shown by spectroscopy. Scanning electron microscopy results demonstrated that bead-free nanofibers with uniform morphology could be obtained by 10% w/v concentrations of PCL/PLLA and PCL/Gelatin solutions. Microspheres prepared by 15% gelatin concentration and cross-linked with 7.5% glutaraldehyde solution were chosen after in vitro release studies for the incorporation to the double-layer matrices. The optimized conditions were used to prepare fibroblast growth factor-2 (FGF-2) loaded microspheres. Preliminary cell culture studies showed that the FGF-2 could be actively loaded into the microspheres and enhanced the cell attachment and proliferation. The complete system had a slow degradation rate in saline (18% weight loss in 2 months) and it could meanwhile preserve its integrity. This sandwich system prevented microsphere leakage from the scaffold, and the hydrophilic and bioactive nature of the fibers at the upper layer promoted cell attachment to the surface. PLLA/PCL layer, on the other hand, improved the mechanical properties of the system and enabled better handling. © 2013 Wiley Periodicals, Inc.
Anahtar Kelimeler : biodegradable scaffold, controlled growth factor release, electrospun nanofibers, article

ISSN: 15493296
7
Shell-core imprinted polyacrylamide crosslinked chitosan for albumin removal from plasma
Dergi Makalesi

Dergi Makalesi
Kaynak :
DSpace Makaleler
Dergi:
Journal of Biomedical Materials Research Part A
Yayıncı:
Wiley-Liss
Yazar(lar) :
Okutucu Burcu , Zihnioglu Figen , Telefoncu Azmi
Konu :
albumin removal, molecular imprinting, chitosan beads
Yıl :
2008
Journal of Biomedical Materials Research Part A
Yayıncı Web Sitesi
Kaydet
Molecularly imprinted polymeric beads were prepared using albumin as the imprinted molecule, acrylamide as the functional monomer, and epichlorhydrin crosslinked chitosan beads as the supporting matrix. The recognition and binding of the imprinted beads was also tested with human plasma for the targeted removal of HSA. Plasma is a rich source of biochemical products that can act as biomarkers of disease or physiological status of a patient. The application of current proteomic technologies in the search for potential diagnostic/prognostic indicators in the plasma of patients is limited by highly abundant albumin that constitute > 60% of the total plasma proteins. Removal of abundant proteins will help in the discovery and detection of less abundant proteins that may prove to be informative. The adsorption capacities of the imprinted polymeric beads for pure human serum albumin and plasma albumin were estimated as 92% and 80%, respectively. The easy preparation protocol of derivatised beads and good protein recognition properties make them attractive for biotechnologic approaches. (c) 2007 Wiley Periodicals, Inc.
Anahtar Kelimeler : albumin removal, molecular imprinting, chitosan beads, article
Cilt: 84A
Sayı: 3
ISSN: 1549-3296
Sınıflandırma :
8
Cornea engineering on polyester carriers
Dergi Makalesi

Dergi Makalesi
Open Access
Kaynak :
DSpace Makaleler
Dergi:
Journal of Biomedical Materials Research - Part A
Yayıncı:
John Wiley & Sons, Inc.
Yazar(lar) :
P. Zorlutuna , A. Tezcaner , I. Kiyat , Atilla Aydınlı , V. Hasirci
Konu :
Cornea, Fibroblasts, Polyester, Retinal pigment epithelial cells, Tissue engineering, Biocompatibility, Biodegradation, Cell culture, Cells, Collagen, Foams, Histology, Polyesters, Porosity, Scanning electron microscopy, Tissue culture, Vision, Epithelial cells, Fibroblasts, Polyester film, Retinal pigment, Biomedical engineering, Collagen type 1, poly(3 hydroxybutyric acid co 3 hydroxyvaleric acid), poly(3 hydroxybutyric acid), Polylactide, Unclassified drug, Animal cell, Biocompatibility, Mouse, Nonhuman, Phenotype, Pigment epithelium, Scanning electron microscopy, Thickness
Yıl :
2006
Journal of Biomedical Materials Research - Part A
Yayıncı Web Sitesi
Tam Metin
Kaydet
In this study, biodegradable polyester based carriers were designed for tissue engineering of the epithelial and the stromal layers of the cornea, and the final construct was tested in vitro. In the construction of the epithelial layer, micropatterned films were prepared from blends of biodegradable and biocompatible polyesters of natural (PHBV) and synthetic (P(L/DL)LA) origin, and these films were seeded with D407 (retinal pigment epithelial) cells. To improve cell adhesion and growth, the films were coated with fibronectin. To serve as the stromal layer of the cornea, highly porous foams of P(L/DL)LA-PHBV blends were seeded with 3T3 fibroblasts. Cell numbers on the polyester carriers were significantly higher than those on the tissue culture polystyrene control. The cells and the carriers were characterized scanning electron micrographs showed that the foam was highly porous and the pores were interconnected. 3T3 Fibroblasts were distributed quite homogeneously at the seeding site, but probably because of the high thickness of the carrier (∼6 mm); they could not sufficiently populate the core (central parts of the foam) during the test duration. The D407 cells formed multilayers on the micropatterned polyester film. Immunohistochemical studies showed that the cells retained their phenotype during culturing; D407 cells formed tight junctions characteristic of epithelial cells, and 3T3 cells deposited collagen type I into the foams. On the basis of these results, we concluded that the micropatterned films and the foams made of P(L/DL)LA-PHBV blends have a serious potential as tissue engineering carriers for the reconstruction of the epithelial and stromal layers of the cornea.
DOI : 10.1002/jbm.a.30772
Anahtar Kelimeler : Cornea, Fibroblasts, Polyester, Retinal pigment epithelial cells, Tissue engineering, Biocompatibility, Biodegradation, Cell culture, Cells, Collagen, Foams, Histology, Polyesters, Porosity, Scanning electron microscopy, Tissue culture, Vision, Epithelial cells, Fibroblasts, Polyester film, Retinal pigment, Biomedical engineering, Collagen type 1, poly(3 hydroxybutyric acid co 3 hydroxyvaleric acid), poly(3 hydroxybutyric acid), Polylactide, Unclassified drug, Animal cell, Biocompatibility, Mouse, Nonhuman, Phenotype, Pigment epithelium, Scanning electron microscopy, Thickness, Article
Cilt: 79
Sayı: 1
ISSN: 1549-3296
Sayfa: 104-113
Sınıflandırma :
9
Herceptin-decorated salinomycin-loaded nanoparticles for breast tumor targeting
Dergi Makalesi

Dergi Makalesi
Kaynak :
DSpace Makaleler
Dergi:
Journal of Biomedical Materials Research - Part A
Yayıncı:
Zonguldak Bülent Ecevit Üniversitesi
Yazar(lar) :
Aydin R.S.T.
Konu :
drug delivery, herceptin, nanoparticles, salinomycin, targeting
Yıl :
2013
Journal of Biomedical Materials Research - Part A
Yayıncı Web Sitesi
 Piri Tam Metin Bulucu
Kaydet
The use of conventional chemotherapeutic drugs was emerged as challenging for breast cancer therapy, because breast cancer stem cells cannot be destroyed due to their great nature of drug resistance. In this study, a novel nanoparticulate system of Herceptin (HER)-immobilized salinomycin (SAL)-encapsulated poly(lactic-co-glycolic acid) (PLGA) (HER-SAL-PLGA) nanoparticles were constructed and investigated for breast cancer targeting. SAL-encapsulated PLGA nanoparticles were characterized for their particle size, morphology, structural and thermal properties, and drug-encapsulation efficiency. HER-SAL-PLGA nanoparticles were characterized via particle size, surface chemistry, and herceptin-immobilization efficiency. In vitro release studies were performed for both nontargeting and targeting SAL-PLGA nanoparticles, which demonstrated a controlled release of SAL from nanoparticles. Cellular uptake of the HER-SAL-PLGA nanoparticles was assessed by fluorescence and optical microscopy and flow cytometry, which showed that the HER-SAL-PLGA nanoparticles were successfully uptaken by MCF7 cells. In conclusion, this novel drug-delivery system, HER-SAL-PLGA, was suggested as a promising targeting system for breast cancer therapy. © 2012 Wiley Periodicals, Inc.
DOI : 10.1002/jbm.a.34448
Anahtar Kelimeler : drug delivery, herceptin, nanoparticles, salinomycin, targeting, article
Cilt: 101 A
Sayı: 5
ISSN: 1549-3296
Sınıflandırma :
10
In vivo performance of simvastatin-loaded electrospun spiral-wound polycaprolactone scaffolds in reconstruction of cranial bone defects in the rat model
Dergi Makalesi

Dergi Makalesi
Kaynak :
DSpace Makaleler
Dergi:
Journal Of Biomedical Materials Research Part A
Yazar(lar) :
Erhan Piskin , I. Alper Isoglu , Nimet Bolgen , Ibrahim Vargel , Sarah Griffiths , Tarik Cavusoglu , Sarah Cartmell
Konu :
bone reconstruction, cranial defects, animal model, biodegradable scaffolds, poly(epsilon-caprolactone), electrospinning, spiral-wounding, simvastatin
Yıl :
2009
Journal Of Biomedical Materials Research Part A
Yayıncı Web Sitesi
 Piri Tam Metin Bulucu
Kaydet
Reconstruction of large bone defects is still a major problem. Tissue-engineering approaches have become a focus in regeneration of bone. In particular, critical-sized defects do not ossify spontaneously. The use of electrospinning is attracting increasing attention in the preparation of tissue-engineering scaffolds. Recently, acellular scaffolds carrying bioactive agents have been used as scaffolds in "in situ" tissue engineering for soft and hard tissue repair. Poly(epsilon-caprolactone) (PCL) with two different molecular weights were synthesized, and the blends of these two were electrospun into nonwoven membranes composed of nanofibers/micropores. To stimulate bone formation, an active drug, "simvastatin" was loaded either after the membranes were formed or during electrospinning. The matrices were then spiral-wound to produce scaffolds with 3D-structures having both macro- and microchannels. Eight-millimeter diameter critical size cranial defects were created in rats. Scaffolds with or without simvastatin were then implanted into these defects. Samples from the implant sites were removed after 1, 3, and 6 months postimplantation. Bone regeneration and tissue response were followed by X-ray microcomputed tomography and histological analysis. These in vivo results exhibited osseous tissue integration within the implant and mineralized bone restoration of the calvarium. Both microCT and histological data clearly demonstrated that the more successful results were observed with the "simvastatin-containing PCL scaffolds," in which simvastatin was incorporated into the PCL scaffolds during electrospinning. For these samples, bone mineralization was quite significant when compared with the other groups. (C) 2008 Wiley Periodicals, Inc. J Biomed Mater Res 90A: 1137-1151, 2009
DOI : 10.1002/jbm.a.32157
Anahtar Kelimeler : bone reconstruction, cranial defects, animal model, biodegradable scaffolds, poly(epsilon-caprolactone), electrospinning, spiral-wounding, simvastatin, article
Cilt: 90A
Sayı: 4
ISSN: 1549-3296
Sınıflandırma :
Biyotıp %95
11
Cellular localization and biological effects of 20nm-gold nanoparticles
Dergi Makalesi

Dergi Makalesi
Open Access
Kaynak :
DSpace Makaleler
Dergi:
Journal of Biomedical Materials Research Part A
Yazar(lar) :
Gamze Tan , Mehmet Ali Onur
Konu :
Gold Nanoparticles, Cytotoxicity, Cellular Localization, Cell Migration, Angiogenesis, Chorioallantoic Membrane
Yıl :
2018
Journal of Biomedical Materials Research Part A
Yayıncı Web Sitesi
Tam Metin
Kaydet
Gold nanoparticles (AuNPs) have recently emerged as prominent vehicles for many biomedical applications from sensing to delivery. The relevant literature contains conflicting data about the effects of AuNPs on living cells. The aim of present study is the synthesis and characterization of AuNPs at nanoscale, tracking their cellular localization and determining their effects on cell viability, migration and angiogenesis. Within this scope, 20 nm AuNPs were synthesized and characterized using various spectrometric techniques to determine their size, shape and surface properties such as charge and texture. Two main cell types including mouse fibroblast (L929) and human cervix adenocarcinoma (HeLa) were used in the study to compare the biological effects of colloidal gold on both non-cancer and cancer cells. AuNPs were allowed to interact with HeLa cells to determine their intracellular localization. AuNPs were mainly attached to the cell membrane/membranous compartments and to be captured in small amounts in cytoplasmic vacuoles or to be distributed freely in the cytosol. Scratch assay results showed that AuNPs reduced cancer cell migration especially at increasing concentrations. According to the chick chorioallantoic membrane assay, AuNPs exhibited strong anti-angiogenetic properties and can inhibit vascularization during angiogenesis. In addition, the MTT assay confirmed that AuNP-treated cells caused concentration dependent cytotoxic effects on both cell types. As a result, AuNPs not only have inhibitory effects on cancer cells, but also possess antiangiogenic activity, thus making them a multipotent agent for cancer therapy.
Anahtar Kelimeler : Gold Nanoparticles, Cytotoxicity, Cellular Localization, Cell Migration, Angiogenesis, Chorioallantoic Membrane, article
Cilt: 106
Sayı: 6
ISSN: 1549-3296
Sınıflandırma :
12
Effects of bone morphogenetic protein on neonatal rat calvarial osteoblast-like cells: An in vitro study
Dergi Makalesi

Dergi Makalesi
Kaynak :
DSpace Makaleler
Dergi:
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
Yayıncı:
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
Yazar(lar) :
Pervin Vural , Belir Atalay , Yusuf Emes , Buket Aybar , Halim Issever , A. Selhan Kaya , Selame N. Eren , Ayhan Bilir
Konu :
Mühendislik, Bilişim ve Teknoloji (ENG), MÜHENDİSLİK, BİYOMEDİKSEL, Mühendislik, MALZEME BİLİMİ, BİYOMATERYAL, Malzeme Bilimi, Biyomedikal Mühendisliği, Mühendislik ve Teknoloji
Yıl :
2008
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
Yayıncı Web Sitesi
Kaydet
Bone morphogenctic proteins (BMPs) are factors that promote osteoblastic differentiation and osteogenesis. The aim of this study was to examine the behavior of neonatal rat calvarial osteoblast cells cultured on different concentrations of BMP graft materials. Fifty thousand cells per milliliter were seeded and cultured on graft materials for 24 and 48 h. Different concentrations of BMPs (combination of BMPs numbered from 1 to 14) were supplemented to the medium. To evaluate cellular proliferation and differentiation, specimens were examined for DNA synthesis, alkaline phosphatase (ALP) activity, cell numbers, and viability of the cells. Further, transforming growth factor-P, (TGF-beta(1)) and lactate dehydrogenase (LDH) levels were investigated. Morphological appearance of the specimens at 24 and 48 h of incubation was evaluated using scanning electron microcopy. Evaluations of DNA synthesis, cell count, and cell viability data revealed that a significant difference existed at 24 and 48 h (p 0.05). No morphological differences were observed in cell morphology on BMP graft material and the control group. These results indicate that BMPs have an inductive effect on osteoblast differentiation and a possible inhibitory effect in the early phases of cell proliferation. (C) 2008 Wiley Periodicals, Inc.
Anahtar Kelimeler : Mühendislik, Bilişim ve Teknoloji (ENG), MÜHENDİSLİK, BİYOMEDİKSEL, Mühendislik, MALZEME BİLİMİ, BİYOMATERYAL, Malzeme Bilimi, Biyomedikal Mühendisliği, Mühendislik ve Teknoloji, Makale

ISSN: 1549-3296
Sınıflandırma :
13
Osteoselection supported by phase separated polymer blend films
Dergi Makalesi

Dergi Makalesi
Open Access
Kaynak :
DSpace Makaleler
Dergi:
Journal of Biomedical Materials Research Part A
Yazar(lar) :
Hilal Unal Gülsuner , N. A. Gengec , Murat Kılınç , H. Y. Erbil , Ayse B. Tekinay
Konu :
Polymer thin film, Saos-2, Micro-structure, Phase separation, Osteoselection, Human gingival fibroblast
Yıl :
2015
Journal of Biomedical Materials Research Part A
Yayıncı Web Sitesi
Tam Metin
Kaydet
The instability of implants after placement inside the body is one of the main obstacles to clinically succeed in periodontal and orthopedic applications. Adherence of fibroblasts instead of osteoblasts to implant surfaces usually results in formation of scar tissue and loss of the implant. Thus, selective bioadhesivity of osteoblasts is a desired characteristic for implant materials. In this study, we developed osteoselective and biofriendly polymeric thin films fabricated with a simple phase separation method using either homopolymers or various blends of homopolymers and copolymers. As adhesive and proliferative features of cells are highly dependent on the physicochemical properties of the surfaces, substrates with distinct chemical heterogeneity, wettability, and surface topography were developed and assessed for their osteoselective characteristics. Surface characterizations of the fabricated polymer thin films were performed with optical microscopy and SEM, their wettabilities were determined by contact angle measurements, and their surface roughness was measured by profilometry. Long-term adhesion behaviors of cells to polymer thin films were determined by F-actin staining of Saos-2 osteoblasts, and human gingival fibroblasts, HGFs, and their morphologies were observed by SEM imaging. The biocompatibility of the surfaces was also examined through cell viability assay. Our results showed that heterogeneous polypropylene polyethylene/polystyrene surfaces can govern Saos-2 and HGF attachment and organization. Selective adhesion of Saos-2 osteoblasts and inhibited adhesion of HGF cells were achieved on micro-structured and hydrophobic surfaces. This work paves the way for better control of cellular behaviors for adjustment of cell material interactions.
DOI : 10.1002/jbm.a.35164
Anahtar Kelimeler : Polymer thin film, Saos-2, Micro-structure, Phase separation, Osteoselection, Human gingival fibroblast, Article
Cilt: 103
Sayı: 1
ISSN: 1549-3296
Sayfa: 154-161
Sınıflandırma :
14
One-pot facile synthesis of silk sericin-capped gold nanoparticles by UVC radiation: Investigation of stability, biocompatibility, and antibacterial activity
Dergi Makalesi

Dergi Makalesi
Kaynak :
DSpace Makaleler
Dergi:
Journal Of Biomedical Materials Research Part A
Yazar(lar) :
Omer Akturk , Zehra Gun Gok , Ozge Erdemli , Mustafa Yigitoglu
Konu :
antibacterial activity, biocompatibility, Bombyx mori silk sericin, colloidal stability, gold nanoparticles
Yıl :
2019
Journal Of Biomedical Materials Research Part A
Yayıncı Web Sitesi
 Piri Tam Metin Bulucu
Kaydet
Herein, an easy one-pot synthesis method for gold nanoparticles (AuNPs), involving only gold salt and sericin extracted from silkworm cocoon in the presence of ultraviolet C (UVC) radiation, was developed. Nanoparticle formation was confirmed by characteristic surface plasmon resonance peaks at 520-540 nm wavelengths, and the influence of silk sericin on enhancing the colloidal stability of AuNPs was confirmed. Transmission electron microscopy examination showed the average size (<10 nm) and size distribution decreased significantly with higher sericin concentration. No antibacterial activity was observed on Gram-positive Bacillus subtilis or Gram-negative Escherichia coli for sole AuNPs (0.065-0.26 mg/ml), but the conjugation of AuNPs with streptomycin antibiotic decreased significantly the required minimum inhibitory concentration doses, as also confirmed with agar plating, Scanning Electron Microscopy and Atomic Force Microscopy analyses. Furthermore, sericin-capped AuNPs showed high cell viabilities (>100%) and no sign of any detectable apoptosis or necrosis in 1-day incubation. Also, high real-time cell proliferation results of AuNPs competitive with positive control groups implied excellent in vitro biocompatibility. These results evidenced that sericin enhanced the colloidal stability of AuNPs and the biological activities of sericin-capped AuNPs reported here could render them suitable nanoscale vehicles for biomedical applications.
DOI : 10.1002/jbm.a.36771
Anahtar Kelimeler : antibacterial activity, biocompatibility, Bombyx mori silk sericin, colloidal stability, gold nanoparticles, article
Cilt: 107
Sayı: 12
ISSN: 1549-3296
15
PROF. DR. HALÛK BURCUOĞLU’NA ARMAĞAN
Kitap Bölümü

Kitap Bölümü
Kaynak :
DSpace Makaleler
Dergi:
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
Yayıncı:
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
Yazar(lar) :
Cüneyt Pekmez
Konu :
Medeni Hukuk, Law, Social Sciences & Humanities, Hukuk, KANUN, Sosyal Bilimler Genel, Sosyal Bilimler (SOC), Sosyal ve Beşeri Bilimler
Yıl :
2021
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
Yayıncı Web Sitesi
Kaydet
Anahtar Kelimeler : Medeni Hukuk, Law, Social Sciences & Humanities, Hukuk, KANUN, Sosyal Bilimler Genel, Sosyal Bilimler (SOC), Sosyal ve Beşeri Bilimler, Kitapta Bölüm
16
Chemical and topographical modification of PHBV surface to promote osteoblast alignment and confinement
Dergi Makalesi

Dergi Makalesi
Open Access
Kaynak :
DSpace Makaleler
Dergi:
Journal of Biomedical Materials Research - Part A
Yayıncı:
John Wiley & Sons, Inc.
Yazar(lar) :
H. Kenar , A. Kocabas , Atilla Aydınlı , V. Hasirci
Konu :
Bone tissue engineering, Micropatterned films, Osteoblasts, PHBV, Photolithography, Bone, Cell culture, Implants (surgical), Photolithography, Silicon wafers, Cell attachment, Film surface, Micropatterned films, Osteoblasts, Alkaline phosphatase, Calcium phosphate, Fibrinogen, Poly (3 hydroxybutyric acid), Silicon, Valeric acid derivative, Animal cell, Bone regeneration, Cell adhesion, Cellular distribution, Chemical cue, Chemical modification, Controlled study, Mesenchyme cell, Nonhuman, Osteoblast, Rat, Surface property, Tissue engineering, Topography, Acridine orange, Animals, Cell adhesion, Cell count, Cells, cultured, Osteoblasts, Polyesters, Polystyrenes, Rats, Sprague-Dawley, Surface Properties
Yıl :
2008
Journal of Biomedical Materials Research - Part A
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Kaydet
Proper cell attachment and distribution, and thus stronger association in vivo between a bone implant and native tissue will improve the success of the implant. In this study, the aim was to achieve promotion of attachment and uniform distribution of rat mesenchymal stem cell-derived osteoblasts by introducing chemical and topographical cues on poly(3-hydroxybutyrate-co-3- hydroxyvalerate) (PHBV) film surfaces. As the chemical cues, either alkaline phosphatase was covalently immobilized on the film surface to induce deposition of calcium phosphate minerals or fibrinogen was adsorbed to improve cell adhesion. Microgrooves and micropits were introduced on the film surface by negative replication of micropatterned Si wafers. Both chemical cues improved cell attachment and even distribution on the PHBV films, but Fb was more effective especially when combined with the micropatterns. Cell alignment (<10° deviation angle) parallel to chemically modified microgrooves (1, 3, or 8 μm groove width) and on 10 μm-thick Fb lines printed on the unpatterned films was achieved. The cells on unpatterned and 5 μm-deep micropitted films were distributed and oriented randomly. Results of this study proved that microtopographies on PHBV can improve osseointegration when combined with chemical cues, and that microgrooves and cell adhesive protein lines on PHBV can guide selective osteoblast adhesion and alignment.
DOI : 10.1002/jbm.a.31638
Anahtar Kelimeler : Bone tissue engineering, Micropatterned films, Osteoblasts, PHBV, Photolithography, Bone, Cell culture, Implants (surgical), Photolithography, Silicon wafers, Cell attachment, Film surface, Micropatterned films, Osteoblasts, Alkaline phosphatase, Calcium phosphate, Fibrinogen, Poly (3 hydroxybutyric acid), Silicon, Valeric acid derivative, Animal cell, Bone regeneration, Cell adhesion, Cellular distribution, Chemical cue, Chemical modification, Controlled study, Mesenchyme cell, Nonhuman, Osteoblast, Rat, Surface property, Tissue engineering, Topography, Acridine orange, Animals, Cell adhesion, Cell count, Cells, cultured, Osteoblasts, Polyesters, Polystyrenes, Rats, Sprague-Dawley, Surface Properties, Article
Cilt: 85A
Sayı: 4
ISSN: 1549-3296
Sayfa: 1001-1010
17
Voltage and wear debris from Ti-6Al-4V interact to affect cell viability during in-vitro fretting corrosion
Dergi Makalesi

Dergi Makalesi
Kaynak :
Wiley Online Library
Dergi:
Journal of Biomedical Materials Research Part A
Yazar(lar) :
Tiantian Hui , Gregory W. Kubacki , Jeremy L. Gilbert
Yıl :
2017
Journal of Biomedical Materials Research Part A
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Kaydet
DOI : 10.1002/jbm.a.36220
ISSN: 1549-3296
Sayı: 1
Cilt: 106
Sayfa: 160-167
18
Development of a biodegradable bone cement for craniofacial applications
Dergi Makalesi

Dergi Makalesi
Kaynak :
Wiley Online Library
Dergi:
Journal of Biomedical Materials Research Part A
Yazar(lar) :
Allan M. Henslee , Dong-Ho Gwak , Antonios G. Mikos , F. Kurtis Kasper
Yıl :
2012
Journal of Biomedical Materials Research Part A
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Kaydet
DOI : 10.1002/jbm.a.34157
ISSN: 1549-3296
Sayfa: n/a-n/a
19
In vitroneurite guidance effects induced by polylysine pinstripe micropatterns with polylysine background
Dergi Makalesi

Dergi Makalesi
Kaynak :
Wiley Online Library
Dergi:
Journal of Biomedical Materials Research Part A
Yazar(lar) :
Sunghoon Joo , Kyungtae Kang , Yoonkey Nam
Konu :
Metals and Alloys, Biomaterials, Ceramics and Composites, Biomedical Engineering
Yıl :
2015
Journal of Biomedical Materials Research Part A
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Kaydet
DOI : 10.1002/jbm.a.35405
ISSN: 1549-3296
Sayı: 8
Cilt: 103
Sayfa: 2731-2739
20
Bupivacaine‐eluting soy protein structures for controlled release and localized pain relief: An in vitro and in vivo study
Dergi Makalesi

Dergi Makalesi
Kaynak :
Wiley Online Library
Dergi:
Journal of Biomedical Materials Research Part A
Yazar(lar) :
Daniella Goder , Shir Giladi , Ariel Furer , Meital Zilberman
Konu :
Metals and Alloys, Biomaterials, Ceramics and Composites, Biomedical Engineering
Yıl :
2021
Journal of Biomedical Materials Research Part A
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Kaydet
DOI : 10.1002/jbm.a.37163
ISSN: 1549-3296

21
High thrombin concentrations in fibrin sealants induce apoptosis in human keratinocytes
Dergi Makalesi

Dergi Makalesi
Kaynak :
Wiley Online Library
Dergi:
Journal of Biomedical Materials Research Part A
Yazar(lar) :
Alfred Gugerell , Klaudia Schossleitner , Susanne Wolbank , Sylvia Nürnberger , Heinz Redl , Heinz Gulle , Andreas Goppelt , Michaela Bittner , Waltraud Pasteiner
Yıl :
2012
Journal of Biomedical Materials Research Part A
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DOI : 10.1002/jbm.a.34007
ISSN: 1549-3296
Sayı: 5
Cilt: 100A
Sayfa: 1239-1247
22
Effects of Cu-doped 45S5 bioactive glass on the lipid peroxidation-associated growth of human osteoblast-like cellsin vitro
Dergi Makalesi

Dergi Makalesi
Kaynak :
Wiley Online Library
Dergi:
Journal of Biomedical Materials Research Part A
Yazar(lar) :
Lidija Milkovic , Alexander Hoppe , Rainer Detsch , Aldo R. Boccaccini , Neven Zarkovic
Konu :
Metals and Alloys, Biomaterials, Ceramics and Composites, Biomedical Engineering
Yıl :
2013
Journal of Biomedical Materials Research Part A
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DOI : 10.1002/jbm.a.35032
ISSN: 1549-3296
Sayı: 10
Cilt: 102
Sayfa: 3556-3561
23
Cytotoxicity and intracellular fate of PLGA and chitosan-coated PLGA nanoparticles in Madin-Darby bovine kidney (MDBK) and human colorectal adenocarcinoma (Colo 205) cells
Dergi Makalesi

Dergi Makalesi
Kaynak :
Wiley Online Library
Dergi:
Journal of Biomedical Materials Research Part A
Yazar(lar) :
Mihaela Trif , Paula E. Florian , Anca Roseanu , Magdalena Moisei , Oana Craciunescu , Carlos E. Astete , Cristina M. Sabliov
Konu :
Metals and Alloys, Biomaterials, Ceramics and Composites, Biomedical Engineering
Yıl :
2015
Journal of Biomedical Materials Research Part A
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Kaydet
DOI : 10.1002/jbm.a.35498
ISSN: 1549-3296
Sayı: 11
Cilt: 103
Sayfa: 3599-3611
24
Immobilization of Ag nanoparticles/FGF-2 on a modified titanium implant surface and improved human gingival fibroblasts behavior
Dergi Makalesi

Dergi Makalesi
Kaynak :
Wiley Online Library
Dergi:
Journal of Biomedical Materials Research Part A
Yazar(lar) :
Qianli Ma , Shenglin Mei , Kun Ji , Yumei Zhang , Paul K. Chu
Konu :
Metals and Alloys, Biomaterials, Ceramics and Composites, Biomedical Engineering
Yıl :
2011
Journal of Biomedical Materials Research Part A
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Kaydet
DOI : 10.1002/jbm.a.33111
ISSN: 1549-3296
Sayı: 2
Cilt: 98A
Sayfa: 274-286
25
Beneficial effects of hydrocortisone or spironolactone coating on foreign body response to mesh biomaterial in a mouse model
Dergi Makalesi

Dergi Makalesi
Kaynak :
Wiley Online Library
Dergi:
Journal of Biomedical Materials Research Part A
Yazar(lar) :
Christopher J. Brandt , Daniel Kammer , Anette Fiebeler , Uwe Klinge
Konu :
Metals and Alloys, Biomaterials, Ceramics and Composites, Biomedical Engineering
Yıl :
2011
Journal of Biomedical Materials Research Part A
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Kaydet
DOI : 10.1002/jbm.a.33136
ISSN: 1549-3296
Sayı: 3
Cilt: 99A
Sayfa: 335-343
26
Processing of degradable ulvan 3D porous structures for biomedical applications
Dergi Makalesi

Dergi Makalesi
Kaynak :
Wiley Online Library
Dergi:
Journal of Biomedical Materials Research Part A
Yazar(lar) :
Anabela Alves , Rui A. Sousa , Rui L. Reis
Konu :
Metals and Alloys, Biomaterials, Ceramics and Composites, Biomedical Engineering
Yıl :
2012
Journal of Biomedical Materials Research Part A
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Kaydet
DOI : 10.1002/jbm.a.34403
ISSN: 1549-3296
Sayı: 4
Cilt: 101A
Sayfa: 998-1006
27
Preparation and characterization of decellularized tendon slices for tendon tissue engineering
Dergi Makalesi

Dergi Makalesi
Kaynak :
Wiley Online Library
Dergi:
Journal of Biomedical Materials Research Part A
Yazar(lar) :
Liang-Ju Ning , Yi Zhang , Xiao-He Chen , Jing-Cong Luo , Xiu-Qun Li , Zhi-Ming Yang , Ting-Wu Qin
Konu :
Metals and Alloys, Biomaterials, Ceramics and Composites, Biomedical Engineering
Yıl :
2012
Journal of Biomedical Materials Research Part A
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Kaydet
DOI : 10.1002/jbm.a.34083
ISSN: 1549-3296
Sayı: 6
Cilt: 100A
Sayfa: 1448-1456
28
Corneal bioprinting utilizing collagen‐based bioinks and primary human keratocytes
Dergi Makalesi

Dergi Makalesi
Kaynak :
Wiley Online Library
Dergi:
Journal of Biomedical Materials Research Part A
Yazar(lar) :
Daniela F. Duarte Campos , Malena Rohde , Mitchell Ross , Parham Anvari , Andreas Blaeser , Michael Vogt , Claudia Panfil , Gary Hinu2010Fai Yam , Jodhbir S. Mehta , Horst Fischer , Peter Walter , Matthias Fuest
Konu :
Metals and Alloys, Biomaterials, Ceramics and Composites, Biomedical Engineering
Yıl :
2019
Journal of Biomedical Materials Research Part A
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DOI : 10.1002/jbm.a.36702
ISSN: 1549-3296
Sayı: 9
Cilt: 107
Sayfa: 1945-1953
29
Positive role of calcium phosphate ceramics regulated inflammation in the osteogenic differentiation of mesenchymal stem cells
Dergi Makalesi

Dergi Makalesi
Kaynak :
Wiley Online Library
Dergi:
Journal of Biomedical Materials Research Part A
Yazar(lar) :
Jing Wang , Xuening Chen , Xiao Yang , Bo Guo , Danyang Li , Xiangdong Zhu , Xingdong Zhang
Konu :
Metals and Alloys, Biomaterials, Ceramics and Composites, Biomedical Engineering
Yıl :
2020
Journal of Biomedical Materials Research Part A
Yayıncı Web Sitesi
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Kaydet
DOI : 10.1002/jbm.a.36903
ISSN: 1549-3296
Sayı: 6
Cilt: 108
Sayfa: 1305-1320

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