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Coronavirus-SARS-CoV-2: Biology and Problems in rRT-PCR Detection
Dergi Makalesi

Dergi Makalesi
Open Access
Kaynak :
Directory of Open Access Journals (DOAJ)
Dergi:
Borneo Journal of Pharmacy
Yazar(lar) :
Maelita Ramdani Moeis , Anis Puji Rahayu , Nisa Ihsani , Wulan Pertiwi
Yıl :
2020
Anis Puji Rahayu
Yayıncı Web Sitesi
 Piri Tam Metin Bulucu
Kaydet Alıntıla
Coronavirus disease 2019 (COVID-19) first appeared in China in December 2019 and was declared a pandemic by the World Health Organization. COVID-19 is caused by Severe Acute Respiratory Syndrome Corona Virus 2 (SARS-CoV-2), a new virus previously unknown to humans. Here we look at what is known about this virus, the main method for detecting the presence of this virus in a person who is used as a golden standard, and the problems that could arise in this detection method. Understanding the biology of the virus and the strengths and weaknesses of the detection method are important for patient management and for overcoming the pandemic.
DOI : 10.33084/bjop.v3iSpecial-1.1429
Anahtar Kelimeler : covid-19, sars-cov-2, rrt-pcr, detection, false results
ISSN: 2621-4814
Cilt: 3
Sayı: Special-1
Sayfa: 136 - 145
Sınıflandırma :
Viroloji %93
2
Unique Characteristics of Recombinant Hybrid Manganese Superoxide Dismutase from Staphylococcus equorum and S. saprophyticus
Dergi Makalesi

Dergi Makalesi
Kaynak :
Springer Link
Dergi:
The Protein Journal
Yazar(lar) :
Debbie S. Retnoningrum , Anis Puji Rahayu , Dina Mulyanti , Astrid Dita , Oliver Valerius , Wangsa T. Ismaya
Konu :
Organic Chemistry, Biochemistry, Bioengineering, Analytical Chemistry
Yıl :
2016
Anis Puji Rahayu
Yayıncı Web Sitesi
Tam Metin
Kaydet Alıntıla
A recombinant hybrid of manganese dependent-superoxide dismutase of Staphylococcus equorum and S. saprophyticus has successfully been overexpressed in Escherichia coli BL21(DE3), purified, and characterized. The recombinant enzyme suffered from degradation and aggregation upon storage at −20 °C, but not at room temperature nor in cold. Chromatographic analysis in a size exclusion column suggested the occurrence of dimeric form, which has been reported to contribute in maintaining the stability of the enzyme. Effect of monovalent (Na+, K+), divalent (Ca2+, Mg2+), multivalent (Mn2+/4+, Zn2+/4+) cations and anions (Cl−, SO4 2−) to the enzyme stability or dimeric state depended on type of cation or anion, its concentration, and pH. However, tremendous effect was observed with 50 mM ZnSO4, in which thermostability of both the dimer and monomer was increased. Similar situation was not observed with MnSO4, and its presence was detrimental at 200 mM. Finally, chelating agent appeared to destabilize the dimer around neutral pH and dissociate it at basic pH. The monomer remained stable upon addition of ethylene diamine tetraacetic acid. Here we reported unique characteristics and stability of manganese dependent-superoxide dismutase from S. equorum/saprophyticus.
DOI : 10.1007/s10930-016-9650-5
Anahtar Kelimeler : Dimer, Manganese dependent-superoxide dismutase, Monomer, Recombinant hybrid protein, Staphylococcus equorum, Thermal stability
ISSN: 1572-3887 1573-4943
Sayı: 2
Cilt: 35
Sayfa: 136-144

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