Piri Keşif Aracı

Ek Bilgiler

DOI : 10.1016/j.ebiom.2021.103580

ISSN: 2352-3964
Cilt: 71
Sayfa: 103580

2

Potential roles of the chaperonin (HtpB), polyamines, and the polyamine binding protein (PotD) in Legionella pneumophila pathogenesis

Tez

Kaynak 1:

NDTLD Thesis

Kaynak 2:

Dissertation.com

Dergi:

Global ETD Search

Yayıncı:

Global ETD Search

Yazar(lar) :

Gheyath K. Nasrallah

Yıl :

2011

Gheyath K. Nasrallah

Özet Ek Bilgiler

The intracellular pathogen Legionella pneumophila replicates in a membrane-bound compartment known as the Legionella-containing vacuole (LCV) where it abundantly releases its chaperonin HtpB, suggesting that HtpB may have virulence-related functions. To assess these functions, I attempted to construct an L. pneumophila ?htpBmutant but was unable to do so, likely because htpB is essential. In the absence of genetic deletion, functional tests were used to study the released HtpB. A small portion of the HtpB in L. pneumophila-infected cells was found in the cytoplasm of the infected cells, as judged by the CyaA reporter assay. To identify potential functions of the HtpB present in the eukaryotic cytoplasm, htpB was ectopically expressed in Saccharomyces cerevisiae. HtpB induced pseudohyphal growth (PHG) in yeast, suggesting it interacts with eukaryotic targets. A yeast two-hybrid screen showed that HtpB interacted with SAMDC, an essential yeast enzyme encoded by SPE2 that is required for polyamine biosynthesis. Overexpression of SPE2 induced PHG in S. cerevisiae, suggesting that HtpB induces PHG by activating polyamine synthesis, and that L. pneumophila may require exogenous polyamines for growth. A pharmacological inhibitor of SAMDC reduced L. pneumophila replication in host cells, whereas exogenous polyamines enhanced intracellular growth. Bioinformatics revealed that most known enzymes required for polyamine biosynthesis in bacteria are absent in L. pneumophila, suggesting that L. pneumophila depends on exogenous polyamines transported from host cells. L. pneumophila possesses only one putative operon,potABCD, which encodes a polyamine transporter. Using GFP as a reporter of potABCD promoter (PpotA), we found that PpotA activity was turned on during exponential phase of growth in vitro. To test the potential function of this transporter in pathogenesis, potD was deleted.Although deletion of potD did not affect L. pneumophila growth in vitro, it reduced L. pneumophila attachment to phagocytic cells, intracellular growth, and the ability of the LCV to recruit vesicles. Collectively, these findings have contributed to a better understanding of the biology of L. pneumophila by suggesting that HtpB and PotD might collaborate to ensure a supply of polyamines required for the optimal intracellular growth of L. pneumophila.

Kaynak 1: http://search.ndltd.org/show.php?id=oai%3Aunion.ndltd.org%3ALACETR%2Foai%3Acollectionscanada.gc.ca%3ANSHD.ca%2310222%2F14200&back=http%3A%2F%2Fsearch.ndltd.org%2Fsearch.php%3Fq%3D%26source_set_names%3D%26year_start%3D%26year_end%3D%26language%255B0%255D%3Denglish%26language%255B1%255D%3Den_us%26start%3D114740 arayıcılığı görüntüle.
Kaynak 2: http://www.dissertation.com/abstracts/1903332 arayıcılığı görüntüle.

Sınıflandırma :

Kimya %90

Fiziksel kimya %99

3

The incidence of the novel coronavirus SARS-CoV-2 among asymptomatic patients: A systematic review

Dergi Makalesi

Kaynak 1:

Science Direct

Kaynak 2:

Directory of Open Access Journals (DOAJ)

Dergi:

International Journal of Infectious Diseases

Yayıncı:

Elsevier

Yazar(lar) :

Duaa W. Al-Sadeq , Gheyath K. Nasrallah

Yıl :

2020

Gheyath K. Nasrallah

Ek Bilgiler

Kaynak 1: http://dx.doi.org/10.1016/j.ijid.2020.06.098 arayıcılığı görüntüle.
Kaynak 2: http://www.sciencedirect.com/science/article/pii/S1201971220305336 arayıcılığı görüntüle.

DOI : 10.1016/j.ijid.2020.06.098

ISSN: 1201-9712
Cilt: 98
Sayfa: 372-380

Sınıflandırma :

Tıp ve Halk Sağlığı %94

Dahiliye %37

4

The Spectrum of Mutations of Homocystinuria in the MENA Region

Dergi Makalesi

Kaynak 1:

MDPI

Kaynak 2:

Directory of Open Access Journals (DOAJ)

Yazar(lar) :

Duaa W. Al-Sadeq , Gheyath K. Nasrallah

Yıl :

2020

Gheyath K. Nasrallah

Özet Ek Bilgiler

Homocystinuria is an inborn error of metabolism due to the deficiency in cystathionine beta-synthase (CBS) enzyme activity. It leads to the elevation of both homocysteine and methionine levels in the blood and urine. Consequently, this build-up could lead to several complications such as nearsightedness, dislocated eye lenses, a variety of psychiatric and behavioral disorders, as well as vascular system complications. The prevalence of homocystinuria is around 1/200,000 births worldwide. However, its prevalence in the Gulf region, notably Qatar, is exceptionally high and reached 1:1800. To date, more than 191 pathogenic CBS mutations have been documented. The majority of these mutations were identified in Caucasians of European ancestry, whereas only a few mutations from African-Americans or Asians were reported. Approximately 87% of all CBS mutations are missense and do not target the CBS catalytic site, but rather result in unstable misfolded proteins lacking the normal biological function, designating them for degradation. The early detection of homocystinuria along with low protein and methionine-restricted diet is the best treatment approach for all types of homocystinuria patients. Yet, less than 50% of affected individuals show a significant reduction in plasma homocysteine levels after treatment. Patients who fail to lower the elevated homocysteine levels, through high protein-restricted diet or by B6 and folic acid supplements, are at higher risk for cardiovascular diseases, neurodegenerative diseases, neural tube defects, and other severe clinical complications. This review aims to examine the mutations spectrum of the CBS gene, the disease management, as well as the current and potential treatment approaches with a greater emphasis on studies reported in the Middle East and North Africa (MENA) region.

Kaynak 1: http://dx.doi.org/10.3390/genes11030330 arayıcılığı görüntüle.
Kaynak 2: https://doi.org/10.3390/genes11030330 arayıcılığı görüntüle.

DOI : 10.3390/genes11030330

ISSN: 2073-4425
Sayı: 3
Cilt: 11
Sayfa: 330

Sınıflandırma :

Biyotıp %99

Metabolik Hastalıklar %95

5

D-Dimer Concentrations and COVID-19 Severity: A Systematic Review and Meta-Analysis

Dergi Makalesi

Kaynak :

Directory of Open Access Journals (DOAJ)

Dergi:

Frontiers in Public Health

Yazar(lar) :

Panagiotis Paliogiannis , Arduino Aleksander Mangoni , Paola Dettori , Gheyath K. Nasrallah , Gheyath K. Nasrallah , Gianfranco Pintus , Gianfranco Pintus , Angelo Zinellu

Yıl :

2020

Gheyath K. Nasrallah Gheyath K. Nasrallah

Piri Tam Metin Bulucu Özet Ek Bilgiler

Coronavirus disease 2019 (COVID-19) is a recently described infectious disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Since late 2019, COVID-19 has rapidly spread in virtually all countries, imposing the adoption of significant lockdown and social distancing measures. The activation of the coagulation cascade is a common feature of disseminated intravascular coagulation and adverse clinical outcomes in COVID-19 patients. In this study, we conducted a meta-analysis aiming to investigate differences in serum D-dimer concentrations in patients with and without severe COVID-19 disease. An electronic search in Medline (PubMed), Scopus and Web of Science was performed with no language restrictions, and 13 articles were reporting on 1,807 patients (585, 32.4% with severe disease) were finally identified and included in the meta-analysis. The pooled results of all studies revealed that the D-dimer concentrations were significantly higher in patients with more severe COVID-19 (SMD: 0.91 mg/L; 95% CI, 0.75 to 1.07 mg/L, p < 0.0001). The heterogeneity was moderate (I2 = 46.5%; p = 0.033). Sensitivity analysis showed that the effect size was not modified when any single study was in turn removed (effect size range, 0.87 mg/L to 0.93 mg/L). The Begg's (p = 0.76) and Egger's tests (p = 0.38) showed no publication bias. In conclusion, our systematic review and meta-analysis showed that serum D-dimer concentrations in patients with severe COVID-19 are significantly higher when compared to those with non-severe forms.

DOI : 10.3389/fpubh.2020.00432

Anahtar Kelimeler : D-dimer, coagulation, thrombosis, COVID-19, SARS-CoV-2

ISSN: 2296-2565
Cilt: 8

6

Enhanced detection of respiratory syncytial virus by shell vial in children hospitalised with respiratory illnesses in Northern Jordan

Dergi Makalesi

Kaynak :

Wiley Online Library

Dergi:

Journal of Medical Virology

Yazar(lar) :

Mamdoh M. Meqdam , Gheyath K. Nasrallah

Konu :

Virology, Infectious Diseases

Yıl :

2000

Gheyath K. Nasrallah

Ek Bilgiler

DOI : 10.1002/1096-9071(200012)62:4<518::aid-jmv18>3.0.co;2-p

ISSN: 0146-6615
Sayı: 4
Cilt: 62
Sayfa: 518-523

7

A comprehensive review on the anti-cancer properties and mechanisms of action of sesamin, a lignan in sesame seeds (Sesamum indicum)

Dergi Makalesi

Kaynak :

Science Direct

Dergi:

European Journal of Pharmacology

Yayıncı:

Elsevier

Yazar(lar) :

Amin F. Majdalawieh , Mariam Massri , Gheyath K. Nasrallah

Yıl :

2017

Gheyath K. Nasrallah

Ek Bilgiler

DOI : 10.1016/j.ejphar.2017.10.020

ISSN: 0014-2999
Cilt: 815
Sayfa: 512-521

Sınıflandırma :

Kimya %95

Yemek Bilimi %100

8

Epstein–Barr Virus Epidemiology, Serology, and Genetic Variability of LMP-1 Oncogene Among Healthy Population: An Update

Dergi Makalesi

Kaynak :

Directory of Open Access Journals (DOAJ)

Dergi:

Frontiers in Oncology

Yazar(lar) :

Maria K. Smatti , Duaa W. Al-Sadeq , Nadima H. Ali , Gianfranco Pintus , Haissam Abou-Saleh , Gheyath K. Nasrallah , Gheyath K. Nasrallah

Yıl :

2018

Gheyath K. Nasrallah Gheyath K. Nasrallah

Piri Tam Metin Bulucu Özet Ek Bilgiler

The Epstein–Barr virus (EBV) is a DNA lymphotropic herpesvirus and the causative agent of infectious mononucleosis. EBV is highly prevalent since it affects more than 90% of individuals worldwide and has been linked to several malignancies including PTLDs, which are one of the most common malignancies following transplantation. Among all the EBV genes, most of the recent investigations focused on studying the LMP-1 oncogene because of its high degree of polymorphism and association with tumorigenic activity. There are two main EBV genotypes, Type 1 and 2, distinguished by the differences in the EBNA-2 gene. Further sub genotyping can be characterized by analyzing the LMP-1 gene variation. The virus primarily transmits through oral secretions and persists as a latent infection in human B-cells. However, it can be transmitted through organ transplantations and blood transfusions. In addition, symptoms of EBV infection are not distinguishable from other viral infections, and therefore, it remains questionable whether there is a need to screen for EBV prior to blood transfusion. Although the process of leukoreduction decreases the viral copies present in the leukocytes, it does not eliminate the risk of EBV transmission through blood products. Here, we provide a review of the EBV epidemiology and the genetic variability of the oncogene LMP-1. Then, we underscore the findings of recent EBV seroprevalence and viremia studies among blood donors as a highly prevalent transfusion transmissible oncovirus.

DOI : 10.3389/fonc.2018.00211

Anahtar Kelimeler : blood donors, Epsteinu2013Barr virus, LMP-1 oncogene, seroprevalence, transfusion, viremia

ISSN: 2234-943X
Cilt: 8

Sınıflandırma :

Biyotıp %100

Viroloji %67

Hematoloji %20

9

Negative epidemiological association between HSV-1 and HSV-2 infections

Dergi Makalesi

Kaynak 1:

Directory of Open Access Journals (DOAJ)

Kaynak 2:

Science Direct

Dergi:

Heliyon

Yazar(lar) :

Gheyath K. Nasrallah , Soha R. Dargham , Laith J. Abu-Raddad

Yıl :

2019

Gheyath K. Nasrallah

Özet Ek Bilgiler

Objectives: Existing evidence on an epidemiological association between herpes simplex virus (HSV) type 1 and type 2 infections remains conflicting and inconclusive. Using a multi-national database of HSV-1/2 serological testing, we aimed to assess the existence of an association between both infections. Design, Setting, and Participants: An HSV-1/2 cross-sectional serological testing database was assembled by merging databases of seroprevalence studies on men blood donors residing currently in Qatar, but from different countries. Specimens were tested for anti-HSV-1 IgG antibodies using HerpeSelect® 1 ELISA, and for anti-HSV-2 IgG antibodies following a two-test algorithm: HerpeSelect® 2 ELISA to test the sera, and Euroline-WB to confirm positive and equivocal specimens. Logistic regressions were conducted to estimate unadjusted and adjusted infection odds ratios. Results: Serological testing for HSV-1/2 was performed on 2522 specimens. Sero-positivity for HSV-1 and HSV-2 was identified in 2053 (81.5%) and 87 (3.5%) specimens, respectively. Univariable analyses estimated higher odds of HSV-2 infection with increasing age and increasing country income level, and an unadjusted odds ratio with HSV-1 sero-positivity of 0.71 (95% CI 0.43–1.17; p-value 0.172). Adjusting for age and country income level, the adjusted odds ratio of HSV-2 infection with HSV-1 sero-positivity was 0.51 (95% CI 0.30–0.87; p-value 0.013). Sensitivity analyses confirmed this association. Conclusions: There is a negative association between HSV-1 and HSV-2 infections, suggestive of a protective effect for HSV-1 sero-positivity against HSV-2 acquisition. This finding supports earlier pooled but inconclusive evidence from prospective studies, yet contrasts with pooled findings of earlier cross-sectional studies.

Kaynak 1: http://www.sciencedirect.com/science/article/pii/S2405844019362097 arayıcılığı görüntüle.
Kaynak 2: http://dx.doi.org/10.1016/j.heliyon.2019.e02549 arayıcılığı görüntüle.

Anahtar Kelimeler : Virology, Epidemiology, Infectious disease, Prevalence, Interaction

ISSN: 2405-8440
Cilt: 5
Sayı: 10
Sayfa: e02549

Sınıflandırma :

Biyotıp %97

Epidemiyoloji %99

10

Recent advances in functional nanostructures as cancer photothermal therapy.

Dergi Makalesi

Kaynak :

PubMed

Dergi:

Int J Nanomedicine

Yazar(lar) :

Essraa A Hussein , Moustafa M Zagho , Gheyath K Nasrallah , Ahmed A Elzatahry

Konu :

Animals, Gold, Humans, Hyperthermia, Induced, Infrared Rays, Magnetite Nanoparticles, Nanostructures, Neoplasms, Phototherapy

Yıl :

2018

Gheyath K Nasrallah

Piri Tam Metin Bulucu Özet Ek Bilgiler

Being a non-invasive and relatively safe technique, photothermal therapy has attracted a lot of interest in the cancer treatment field. Recently, nanostructure technology has entered the forefront of cancer therapy owing to its ability to absorb near-infrared radiation as well as efficient light to heat conversion. In this study, key nanostructures for cancer therapy including gold nanoparticles, magnetite iron oxide nanoparticles, organic nanomaterials, and novel two-dimensional nanoagents such as MXenes are discussed. Furthermore, we briefly discuss the characteristics of the nanostructures of these photothermal nanomaterial agents, while focusing on how nanostructures hold potential as cancer therapies. Finally, this review offers promising insight into new cancer therapy approaches, particularly in vivo and in vitro cancer treatments.

DOI : 10.2147/IJN.S161031

Anahtar Kelimeler : cancer therapy, nanostructures, near-infrared, photothermal therapy, plasmonic

Sınıflandırma :

Malzeme Bilimi %84

Nanoteknoloji %52

Doku Mühendisliği %45

11

Synthesis, Bioapplications, and Toxicity Evaluation of Chitosan-Based Nanoparticles

Dergi Makalesi

Kaynak 1:

Directory of Open Access Journals (DOAJ)

Kaynak 2:

MDPI

Dergi:

International Journal of Molecular Sciences

Yazar(lar) :

Balsam R. Rizeq , Nadin N. Younes , Kashif Rasool , Gheyath K. Nasrallah

Yıl :

2019

Gheyath K. Nasrallah

Piri Tam Metin Bulucu Özet Ek Bilgiler

The development of advanced nanomaterials and technologies is essential in biomedical engineering to improve the quality of life. Chitosan-based nanomaterials are on the forefront and attract wide interest due to their versatile physicochemical characteristics such as biodegradability, biocompatibility, and non-toxicity, which play a promising role in biological applications. Chitosan and its derivatives are employed in several applications including pharmaceuticals and biomedical engineering. This article presents a comprehensive overview of recent advances in chitosan derivatives and nanoparticle synthesis, as well as emerging applications in medicine, tissue engineering, drug delivery, gene therapy, and cancer therapy. In addition to the applications, we critically review the main concerns and mitigation strategies related to chitosan bactericidal properties, toxicity/safety using tissue cultures and animal models, and also their potential environmental impact. At the end of this review, we also provide some of future directions and conclusions that are important for expanding the field of biomedical applications of the chitosan nanoparticles.

Kaynak 1: https://doi.org/10.3390/ijms20225776 arayıcılığı görüntüle.
Kaynak 2: http://dx.doi.org/10.3390/ijms20225776 arayıcılığı görüntüle.

DOI : 10.3390/ijms20225776

Anahtar Kelimeler : chitosan, nanoparticles, biomedical, pharmaceuticals, toxicity

Cilt: 20
Sayı: 22
Sayfa: 5776

Sınıflandırma :

Kimya %79

Biyomedikal Mühendisliği ve Biyoteknoloji %93

12

Systematic Review of the Respiratory Syncytial Virus (RSV) Prevalence, Genotype Distribution, and Seasonality in Children from the Middle East and North Africa (MENA) Region

Dergi Makalesi

Kaynak 1:

Directory of Open Access Journals (DOAJ)

Kaynak 2:

MDPI

Dergi:

Microorganisms

Yazar(lar) :

Hadi M. Yassine , Muhammad U. Sohail , Nadin Younes , Gheyath K. Nasrallah

Yıl :

2020

Gheyath K. Nasrallah

Piri Tam Metin Bulucu Özet Ek Bilgiler

Respiratory syncytial virus (RSV) is one of the most common viruses to infect children worldwide and is the leading cause of lower respiratory tract illness (LRI) in infants. This study aimed to conduct a systematic review by collecting and reviewing all the published knowledge about the epidemiology of RSV in the Middle East and North Africa (MENA) region. Therefore, we systematically searched four databases; Embase, Medline, Scopus, and Cochrane databases from 2001 to 2019 to collect all the information related to the RSV prevalence, genotype distribution, and seasonality in children in MENA region. Our search strategy identified 598 studies, of which 83 met our inclusion criteria, which cover the past 19 years (2000–2019). Odds ratio (OR) and confidence interval (CI) were calculated to measure the association between RSV prevalence, gender, and age distribution. An overall prevalence of 24.4% (n = 17,106/69,981) of respiratory infections was recorded for RSV. The highest RSV prevalence was reported in Jordan (64%, during 2006–2007) and Israel (56%, 2005–2006). RSV A subgroup was more prevalent (62.9%; OR = 2.9, 95%CI = 2.64–3.13) than RSV B. RSV was most prevalent in children who were less than 12 months old (68.6%; OR = 4.7, 95%CI = 2.6–8.6) and was higher in males (59.6%; OR = 2.17, 95%CI = 1.2–3.8) than in female infants. Finally, the highest prevalence was recorded during winter seasons in all countries, except for Pakistan. RSV prevalence in the MENA region is comparable with the global one (25.5% vs. 22%). This first comprehensive report about RSV prevalence in the MENA region and our data should be important to guide vaccine introduction decisions and future evaluation.

Kaynak 1: https://doi.org/10.3390/microorganisms8050713 arayıcılığı görüntüle.
Kaynak 2: http://dx.doi.org/10.3390/microorganisms8050713 arayıcılığı görüntüle.

DOI : 10.3390/microorganisms8050713

Anahtar Kelimeler : prevalence, genotype, children age, season

ISSN: 2076-2607
Cilt: 8
Sayı: 713
Sayfa: 713

Sınıflandırma :

Biyotıp %97

Viroloji %100

13

Potential roles of the chaperonin (HtpB), polyamines, and the polyamine binding protein (PotD) in Legionella pneumophila pathogenesis /by Gheyath K. Nasrallah.

Tez

Kaynak 1:

Canada Theses

Kaynak 2:

Canada Theses

Dergi:

Halifax, NS :Dalhousie University

Yayıncı:

Halifax, NS :Dalhousie University

Yazar(lar) :

Gheyath K Nasrallah

Yıl :

2011

Gheyath K Nasrallah

Özet Ek Bilgiler

ABSTRACT: The intracellular pathogen Legionella pneumophila replicates in a membrane-bound compartment known as the Legionella-containing vacuole (LCV) where it abundantly releases its chaperonin HtpB, suggesting that HtpB may have virulence-related functions. To assess these functions, I attempted to construct an L. pneumophila?htpBmutant but was unable to do so, likely because htpB is essential. In the absence of genetic deletion, functional tests were used to study the released HtpB. A small portion of the HtpB in L. pneumophila-infected cells was found in the cytoplasm of the infected cells, as judged by the CyaA reporter assay. To identify potential functions of the HtpB present in the eukaryotic cytoplasm, htpB was ectopically expressed in Saccharomyces cerevisiae. HtpB induced pseudohyphal growth (PHG) in yeast, suggesting it interacts with eukaryotic targets. A yeast two-hybrid screen showed that HtpB interacted with SAMDC, an essential yeast enzyme encoded by SPE2 that is required for polyamine biosynthesis. Overexpression of SPE2 induced PHG in S. cerevisiae, suggesting that HtpB induces PHG by activating polyamine synthesis, and that L. pneumophila may require exogenous polyamines for growth. A pharmacological inhibitor of SAMDC reduced L. pneumophila replication in host cells, whereas exogenous polyamines enhanced intracellular growth. Bioinformatics revealed that most known enzymes required for polyamine biosynthesis in bacteria are absent in L. pneumophila, suggesting that L. pneumophila depends on exogenous polyamines transported from host cells. L. pneumophila possesses only one putative operon, potABCD, which encodes a polyamine transporter. Using GFP as a reporter of potABCD promoter (PpotA), we found that PpotA activity was turned on during exponential phase of growth in vitro. To test the potential function of this transporter in pathogenesis, potD was deleted.

Kaynak 1: https://www.bac-lac.gc.ca/eng/services/theses/Pages/item.aspx?idNumber=864662200 arayıcılığı görüntüle.
Kaynak 2: https://www.bac-lac.gc.ca/eng/services/theses/Pages/item.aspx?idNumber=864662200 arayıcılığı görüntüle.

Sınıflandırma :

Kimya %94

Fiziksel kimya %99

14

Severe acute respiratory syndrome coronavirus‐2 natural animal reservoirs and experimental models: systematic review

Dergi Makalesi

Kaynak :

Wiley Online Library

Dergi:

Reviews in Medical Virology

Yazar(lar) :

Salma Younes , Nadin Younes , Farah Shurrab , Gheyath K. Nasrallah

Konu :

Virology, Infectious Diseases

Yıl :

2020

Gheyath K. Nasrallah

Ek Bilgiler

DOI : 10.1002/rmv.2196

ISSN: 1052-9276

15

The Legionella pneumophila chaperonin – an unusual multifunctional protein in unusual locations

Dergi Makalesi

Kaynak :

Directory of Open Access Journals (DOAJ)

Dergi:

Frontiers in Cellular and Infection Microbiology

Yazar(lar) :

Rafael A. eGarduno , Audrey eChong , Gheyath K. Nasrallah , David S. Allan

Yıl :

2011

Gheyath K. Nasrallah

Piri Tam Metin Bulucu Özet Ek Bilgiler

The L. pneumophila chaperonin, HtpB, was discovered as a highly immunogenic antigen, only a few years after the identification of L. pneumophila as the causative agent of Legionnaires’ disease. As its counterparts in other bacterial pathogens, HtpB did not initially receive further attention, particularly because research was focused on a few model chaperonins that were used to demonstrate that chaperonins are essential stress proteins, present in all cellular forms of life and involved in helping other proteins to fold. However, chaperonins have recently attracted increasing interest, particularly after several reports confirmed their multifunctional nature and the presence of multiple chaperonin genes in numerous bacterial species. It is now accepted that bacterial chaperonins are capable of playing a variety of protein folding-independent roles. HtpB is clearly a multifunctional chaperonin that according to its location in the bacterial cell, or in the L. pneumophila-infected cell, plays different roles. HtpB exposed on the bacterial cell surface can act as an invasion factor for non-phagocytic cells, whereas the HtpB released in the host cell can act as an effector capable of altering organelle trafficking, the organization of actin microfilaments and cell signaling pathways. The road to discover the multifunctional nature of HtpB has been exciting and here we provide a historical perspective of the key findings linked to such discovery, as well as a summary of the experimental work (old and new) performed in our laboratory. Our current understanding has led us to propose that HtpB is an ancient protein that L. pneumophila uses as a key molecular tool important to the intracellular establishment of this fascinating pathogen.

DOI : 10.3389/fmicb.2011.00122

Anahtar Kelimeler : Mitochondria, Polyamines, Pathogenesis, microfilaments, HSP60, GroEL

ISSN: 2235-2988
Cilt: 2

Sınıflandırma :

Biyotıp %93

İlaç Direnci %60

İnsan Genetiği %20

16

Resveratrol Inhibits Oxidative Stress and Prevents Mitochondrial Damage Induced by Zinc Oxide Nanoparticles in Zebrafish (Danio rerio)

Dergi Makalesi

Kaynak 1:

Directory of Open Access Journals (DOAJ)

Kaynak 2:

MDPI

Dergi:

International Journal of Molecular Sciences

Yazar(lar) :

Roberta Giordo , Gheyath K. Nasrallah , Ola Al-Jamal , Panagiotis Paliogiannis , Gianfranco Pintus

Yıl :

2020

Gheyath K. Nasrallah

Piri Tam Metin Bulucu Özet Ek Bilgiler

Despite their wide industrial use, Zinc oxide (ZnO) nanoparticles (NPs) exhibit a high toxic potential while concerns of their health-related risks are still present, urging additional in vivo clarification studies. Oxidative stress is recognized as the primary trigger of NP-associated toxicity, suggesting antioxidants as a promising counteractive approach. Here, we investigated the protective effect of the natural antioxidant resveratrol against ZnO NP-induced toxicity in vivo using the zebrafish model. Our findings demonstrate that resveratrol counteracts ZnO NP-induced zebrafish lethality preventing cardiac morphological and functional damage. NP-induced vascular structural abnormalities during embryonic fish development were significantly counteracted by resveratrol treatment. Mechanistically, we further showed that resveratrol inhibits ROS increase, prevents mitochondrial membrane potential dysfunction, and counteracts cell apoptosis/necrosis elicited by ZnO NP. Overall, our data provide further evidence demonstrating the primary role of oxidative stress in NP-induced damage, and highlight new insights concerning the protective mechanism of antioxidants against nanomaterial toxicity.

Kaynak 1: https://doi.org/10.3390/ijms21113838 arayıcılığı görüntüle.
Kaynak 2: http://dx.doi.org/10.3390/ijms21113838 arayıcılığı görüntüle.

DOI : 10.3390/ijms21113838

Anahtar Kelimeler : resveratrol, nanoparticles, oxidative stress, antioxidants, mitochondria

Cilt: 21
Sayı: 3838
Sayfa: 3838

Sınıflandırma :

Biyotıp %99

Apoptoz %87

17

Hepatitis E Viruses

e-Kitap

Kaynak :

Springer Link

Dergi:

Emerging and Transboundary Animal Viruses

Yayıncı:

Springer Link

Yazar(lar) :

Harsh Kumar , Nassim Kamar , Gheyath K. Nasrallah , Dinesh Kumar

Yıl :

2020

Gheyath K. Nasrallah

Özet Ek Bilgiler

Hepatitis E virus (HEV), member of genus Orthohepevirus in the family Hepeviridae, is the leading cause of hepatitis E. More than 20 million cases of hepatitis E virus (HEV) infection have been reported globally, out of which 3.3 million suffered from symptomatic illness and 44,000 deaths were recorded. Hepatitis E is considered highly significant on account of its predominance in both developed and developing nations due to poor sanitation and low-grade drinking water. In India and Africa, the major cause of hepatitis E was water contaminated with faecal matter. Reports from East and Southeast Asia signified the role of animal reservoir, especially pigs, for HEV. In Western Asia, the major cases of hepatitis E were associated with blood transfusion. However, in Europe and South and North America, the significant causes of hepatitis E were associated with the consumption of pork products. Till date, major rely is on the enzyme immunoassays involving antibodies for detection and diagnosis purpose. Still, there is a need for the improvement and development of new approaches and methods for diagnosing hepatitis E virus as well as developing HEV vaccines.

DOI : 10.1007/978-981-15-0402-0_15

Anahtar Kelimeler : HEV, History, Genome and classification, Pathogenesis, Transmission and epidemiology, Diagnosis, Vaccines, Prevention, Zoonoses

ISBN: 978-981-15-0402-0
Sayfa: 357-377

Sınıflandırma :

Biyotıp %100

Parazitoloji ve Enfeksiyon Hastalıkları %100

18

Adipose tissue dysfunction in cancer cachexia

Dergi Makalesi

Kaynak :

Wiley Online Library

Dergi:

Journal of Cellular Physiology

Yazar(lar) :

Sahar I. Daas , Balsam R. Rizeq , Gheyath K. Nasrallah

Yıl :

2018

Gheyath K. Nasrallah

Ek Bilgiler

DOI : 10.1002/jcp.26811

ISSN: 0021-9541
Sayı: 1
Cilt: 234
Sayfa: 13-22

19

Novel role of BRCA1 interacting C‐terminal helicase 1 ( BRIP1) in breast tumour cell invasion

Dergi Makalesi

Kaynak :

Wiley Online Library

Dergi:

Journal of Cellular and Molecular Medicine

Yazar(lar) :

Balsam Rizeq , Saïd Sif , Gheyath K. Nasrallah , Allal Ouhtit

Konu :

Molecular Medicine, Cell Biology

Yıl :

2020

Gheyath K. Nasrallah

Ek Bilgiler

DOI : 10.1111/jcmm.15761

ISSN: 1582-1838
Sayı: 19
Cilt: 24
Sayfa: 11477-11488

20

Seroprevalence and incidence of hepatitis E virus among blood donors: A review

Dergi Makalesi

Kaynak :

Wiley Online Library

Dergi:

Reviews in Medical Virology

Yazar(lar) :

Duaa W. Al-Sadeq , Amin F. Majdalawieh , Gheyath K. Nasrallah

Yıl :

2017

Gheyath K. Nasrallah

Ek Bilgiler

DOI : 10.1002/rmv.1937

ISSN: 1052-9276
Sayı: 5
Cilt: 27
Sayfa: e1937

21

Anti-cancer properties and mechanisms of action of thymoquinone, the major active ingredient ofNigella sativa

Dergi Makalesi

Kaynak :

Taylor and Francis

Dergi:

Critical Reviews in Food Science and Nutrition

Yayıncı:

Informa UK Limited

Yazar(lar) :

Amin F. Majdalawieh , Muneera W. Fayyad , Gheyath K. Nasrallah

Yıl :

2017

Gheyath K. Nasrallah

Ek Bilgiler

DOI : 10.1080/10408398.2016.1277971

ISSN: 1040-8398
Sayı: 18
Cilt: 57
Sayfa: 3911-3928

22

A Potential Link Between Oxidative Stress and Endothelial-to-Mesenchymal Transition in Systemic Sclerosis

Dergi Makalesi

Kaynak :

Directory of Open Access Journals (DOAJ)

Dergi:

Frontiers in Immunology

Yazar(lar) :

Duong Thi Bich Thuan , Hatem Zayed , Ali H. Eid , Ali H. Eid , Ali H. Eid , Haissam Abou-Saleh , Gheyath K. Nasrallah , Gheyath K. Nasrallah , Arduino A. Mangoni , Gianfranco Pintus , Gianfranco Pintus

Yıl :

2018

Gheyath K. Nasrallah Gheyath K. Nasrallah

Piri Tam Metin Bulucu Özet Ek Bilgiler