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Editorial: Arterial Aging and Age-Associated Arterial Diseases
Dergi Makalesi

Dergi Makalesi
Open Access
Kaynak :
Directory of Open Access Journals (DOAJ)
Dergi:
Frontiers in Genetics
Yazar(lar) :
Mingyi Wang , Jiguang Wang , Cuntai Zhang , Gianfranco Pintus , Gianfranco Pintus
Yıl :
2018
Gianfranco Pintus Gianfranco Pintus
Piri Tam Metin Bulucu
Kaydet Alıntıla
DOI : 10.3389/fgene.2018.00444
Anahtar Kelimeler : aging, arterial remodeling, proinflammation, hypertension, atherosclerosis
ISSN: 1664-8021
Cilt: 9
Sınıflandırma :
2
D-Dimer Concentrations and COVID-19 Severity: A Systematic Review and Meta-Analysis
Dergi Makalesi

Dergi Makalesi
Open Access
Kaynak :
Directory of Open Access Journals (DOAJ)
Dergi:
Frontiers in Public Health
Yazar(lar) :
Panagiotis Paliogiannis , Arduino Aleksander Mangoni , Paola Dettori , Gheyath K. Nasrallah , Gheyath K. Nasrallah , Gianfranco Pintus , Gianfranco Pintus , Angelo Zinellu
Yıl :
2020
Gianfranco Pintus Gianfranco Pintus
Yayıncı Web Sitesi
 Piri Tam Metin Bulucu
Kaydet Alıntıla
Coronavirus disease 2019 (COVID-19) is a recently described infectious disease caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Since late 2019, COVID-19 has rapidly spread in virtually all countries, imposing the adoption of significant lockdown and social distancing measures. The activation of the coagulation cascade is a common feature of disseminated intravascular coagulation and adverse clinical outcomes in COVID-19 patients. In this study, we conducted a meta-analysis aiming to investigate differences in serum D-dimer concentrations in patients with and without severe COVID-19 disease. An electronic search in Medline (PubMed), Scopus and Web of Science was performed with no language restrictions, and 13 articles were reporting on 1,807 patients (585, 32.4% with severe disease) were finally identified and included in the meta-analysis. The pooled results of all studies revealed that the D-dimer concentrations were significantly higher in patients with more severe COVID-19 (SMD: 0.91 mg/L; 95% CI, 0.75 to 1.07 mg/L, p < 0.0001). The heterogeneity was moderate (I2 = 46.5%; p = 0.033). Sensitivity analysis showed that the effect size was not modified when any single study was in turn removed (effect size range, 0.87 mg/L to 0.93 mg/L). The Begg's (p = 0.76) and Egger's tests (p = 0.38) showed no publication bias. In conclusion, our systematic review and meta-analysis showed that serum D-dimer concentrations in patients with severe COVID-19 are significantly higher when compared to those with non-severe forms.
DOI : 10.3389/fpubh.2020.00432
Anahtar Kelimeler : D-dimer, coagulation, thrombosis, COVID-19, SARS-CoV-2
ISSN: 2296-2565
Cilt: 8
3
Redox Status and Proteostasis in Ageing and Disease
Dergi Makalesi

Dergi Makalesi
Open Access
Kaynak :
Directory of Open Access Journals (DOAJ)
Dergi:
Oxidative Medicine and Cellular Longevity
Yazar(lar) :
Federica Rizzi , Ioannis P. Trougakos , Gianfranco Pintus , Gerasimos P. Sykiotis
Yıl :
2016
Gianfranco Pintus
Piri Tam Metin Bulucu
Kaydet Alıntıla
DOI : 10.1155/2016/7476241
Cilt: 2016
Sınıflandırma :
4
Crosstalk Between Oxidative Stress and Endoplasmic Reticulum (ER) Stress in Endothelial Dysfunction and Aberrant Angiogenesis Associated With Diabetes: A Focus on the Protective Roles of Heme Oxygenase (HO)-1
Dergi Makalesi

Dergi Makalesi
Open Access
Kaynak :
Directory of Open Access Journals (DOAJ)
Dergi:
Frontiers in Physiology
Yazar(lar) :
Hatem Maamoun , Tarek Benameur , Gianfranco Pintus , Shankar Munusamy , Abdelali Agouni
Yıl :
2019
Gianfranco Pintus
Yayıncı Web Sitesi
 Piri Tam Metin Bulucu
Kaydet Alıntıla
Type-2 diabetes prevalence is continuing to rise worldwide due to physical inactivity and obesity epidemic. Diabetes and fluctuations of blood sugar are related to multiple micro- and macrovascular complications, that are attributed to oxidative stress, endoplasmic reticulum (ER) activation and inflammatory processes, which lead to endothelial dysfunction characterized, among other features, by reduced availability of nitric oxide (NO) and aberrant angiogenic capacity. Several enzymatic anti-oxidant and anti-inflammatory agents have been found to play protective roles against oxidative stress and its downstream signaling pathways. Of particular interest, heme oxygenase (HO) isoforms, specifically HO-1, have attracted much attention as major cytoprotective players in conditions associated with inflammation and oxidative stress. HO operates as a key rate-limiting enzyme in the process of degradation of the iron-containing molecule, heme, yielding the following byproducts: carbon monoxide (CO), iron, and biliverdin. Because HO-1 induction was linked to pro-oxidant states, it has been regarded as a marker of oxidative stress; however, accumulating evidence has established multiple cytoprotective roles of the enzyme in metabolic and cardiovascular disorders. The cytoprotective effects of HO-1 depend on several cellular mechanisms including the generation of bilirubin, an anti-oxidant molecule, from the degradation of heme; the induction of ferritin, a strong chelator of free iron; and the release of CO, that displays multiple anti-inflammatory and anti-apoptotic actions. The current review article describes the major molecular mechanisms contributing to endothelial dysfunction and altered angiogenesis in diabetes with a special focus on the interplay between oxidative stress and ER stress response. The review summarizes the key cytoprotective roles of HO-1 against hyperglycemia-induced endothelial dysfunction and aberrant angiogenesis and discusses the major underlying cellular mechanisms associated with its protective effects.
DOI : 10.3389/fphys.2019.00070
Anahtar Kelimeler : heme oxygenase-1 (Ho-1), endothelial dysfunction, angiogenesis, oxidative stress, ER stress, diabetes
ISSN: 1664-042X
Cilt: 10
Sınıflandırma :
5
Simultaneous determination of citrulline and arginine in human blood plasma by capillary electrophoresis with ultraviolet absorption detection
Dergi Makalesi

Dergi Makalesi
Kaynak :
Wiley Online Library
Dergi:
Journal of Separation Science
Yazar(lar) :
Mauro Forteschi , Salvatore Sotgia , Gianfranco Pintus , Angelo Zinellu , Ciriaco Carru
Konu :
Filtration and Separation, Analytical Chemistry
Yıl :
2014
Gianfranco Pintus
Yayıncı Web Sitesi
Tam Metin
Kaydet Alıntıla
A new capillary electrophoresis method to measure human blood plasma arginine and citrulline levels in a single run without derivatization was established. After adding homoarginine as internal standard, plasma proteins were removed by a 90:10 v/v acetonitrile/ammonia mixture. Arginine and citrulline were detected by an ultraviolet detector at 190 nm and separated in 11.65 and 20.43 min, respectively, by using a 75 mmol/L Tris phosphate solution at pH 1.2 as a background electrolyte. Limits of detection were 0.8 and 5 μmol/L for arginine and citrulline, respectively. Precision tests indicated a good repeatability of migration times and of peak area both for citrulline (CV% = 0.82 and 3.19) and arginine (CV% = 0.65 and 2.79). The CV% for intra‐ and interassay tests were, respectively, 1.84 and 3.23 for citrulline and 1.25 and 1.50 for arginine. Mean recovery was 101.5 and 98.5% for citrulline and arginine, respectively. The performance of the developed method was assessed by measuring plasma arginine levels in 52 subjects and the data were compared with those obtained by our previous assay. The new method was then applied to assess plasma citrulline and arginine in ten chronic kidney disease patients under hypolipidemic therapy with statin.
DOI : 10.1002/jssc.201400177
ISSN: 1615-9306
Sayı: 17
Cilt: 37
Sayfa: 2418-2423
6
Clinical and Biochemical Correlates of Serum L-Ergothioneine Concentrations in Community-Dwelling Middle-Aged and Older Adults
Araştırma Makalesi

Araştırma Makalesi
Open Access
Kaynak :
PLOS - Public Library of Science
Dergi:
Public Library of Science
Yazar(lar) :
Mark McEvoy , Ciriaco Carru , John Attia , Gianfranco Pintus , Arduino A. Mangoni , Angelo Zinellu , Salvatore Sotgia
Yıl :
2014
Gianfranco Pintus
Tam Metin
Kaydet Alıntıla
Background Despite the increasing interest towards the biological role of L-ergothioneine, little is known about the serum concentrations of this unusual aminothiol in older adults. We addressed this issue in a representative sample of community-dwelling middle-aged and older adults. Methods Body mass index, estimated glomerular filtration rate, serum concentrations of L-ergothioneine, taurine, homocysteine, cysteine, glutathione, cysteinylglycine, and glutamylcysteine were evaluated in 439 subjects (age 55–85 years) randomly selected from the Hunter Community Study. Results Median L-ergothioneine concentration in the entire cohort was 1.01 IQR 0.78–1.33 µmol/L. Concentrations were not affected by gender (P = 0.41) or by presence of chronic medical conditions (P = 0.15). By considering only healthy subjects, we defined a reference interval for L-ergothioneine serum concentrations from 0.36 (90% CI 0.31–0.44) to 3.08 (90% CI 2.45–3.76) µmol/L. Using stepwise multiple linear regression analysis L-ergothioneine was negatively correlated with age (rpartial = −0.15; P = 0.0018) and with glutamylcysteine concentrations (rpartial = −0.13; P = 0.0063). Conclusions A thorough analysis of serum L-ergothioneine concentrations was performed in a large group of community-dwelling middle-aged and older adults. Reference intervals were established. Age and glutamylcysteine were independently negatively associated with L-ergothioneine serum concentration.
DOI : 10.1371/journal.pone.0084918
Anahtar Kelimeler : Antioxidants, Thiols, Red blood cells, Elderly, Glutathione, Body mass index, Cysteine, Oxidative stress
Sınıflandırma :
7
Resveratrol Inhibits Oxidative Stress and Prevents Mitochondrial Damage Induced by Zinc Oxide Nanoparticles in Zebrafish (Danio rerio)
Dergi Makalesi

Dergi Makalesi
Open Access
Kaynak :
Directory of Open Access Journals (DOAJ)
Dergi:
International Journal of Molecular Sciences
Yazar(lar) :
Roberta Giordo , Gheyath K. Nasrallah , Ola Al-Jamal , Panagiotis Paliogiannis , Gianfranco Pintus
Yıl :
2020
Gianfranco Pintus
Piri Tam Metin Bulucu
Kaydet Alıntıla
Despite their wide industrial use, Zinc oxide (ZnO) nanoparticles (NPs) exhibit a high toxic potential while concerns of their health-related risks are still present, urging additional in vivo clarification studies. Oxidative stress is recognized as the primary trigger of NP-associated toxicity, suggesting antioxidants as a promising counteractive approach. Here, we investigated the protective effect of the natural antioxidant resveratrol against ZnO NP-induced toxicity in vivo using the zebrafish model. Our findings demonstrate that resveratrol counteracts ZnO NP-induced zebrafish lethality preventing cardiac morphological and functional damage. NP-induced vascular structural abnormalities during embryonic fish development were significantly counteracted by resveratrol treatment. Mechanistically, we further showed that resveratrol inhibits ROS increase, prevents mitochondrial membrane potential dysfunction, and counteracts cell apoptosis/necrosis elicited by ZnO NP. Overall, our data provide further evidence demonstrating the primary role of oxidative stress in NP-induced damage, and highlight new insights concerning the protective mechanism of antioxidants against nanomaterial toxicity.
DOI : 10.3390/ijms21113838
Anahtar Kelimeler : resveratrol, nanoparticles, oxidative stress, antioxidants, mitochondria
Cilt: 21
Sayı: 3838
Sayfa: 3838
Sınıflandırma :
Apoptoz %87
8
A Potential Link Between Oxidative Stress and Endothelial-to-Mesenchymal Transition in Systemic Sclerosis
Dergi Makalesi

Dergi Makalesi
Open Access
Kaynak :
Directory of Open Access Journals (DOAJ)
Dergi:
Frontiers in Immunology
Yazar(lar) :
Duong Thi Bich Thuan , Hatem Zayed , Ali H. Eid , Ali H. Eid , Ali H. Eid , Haissam Abou-Saleh , Gheyath K. Nasrallah , Gheyath K. Nasrallah , Arduino A. Mangoni , Gianfranco Pintus , Gianfranco Pintus
Yıl :
2018
Gianfranco Pintus Gianfranco Pintus
Piri Tam Metin Bulucu
Kaydet Alıntıla
Systemic sclerosis (SSc), an autoimmune disease that is associated with a number of genetic and environmental risk factors, is characterized by progressive fibrosis and microvasculature damage in the skin, lungs, heart, digestive system, kidneys, muscles, joints, and nervous system. These abnormalities are associated with altered secretion of growth factor and profibrotic cytokines, such as transforming growth factor-beta (TGF-β), interleukin-4 (IL-4), platelet-derived growth factor (PDGF), and connective-tissue growth factor (CTGF). Among the cellular responses to this proinflammatory environment, the endothelial cells phenotypic conversion into activated myofibroblasts, a process known as endothelial to mesenchymal transition (EndMT), has been postulated. Reactive oxygen species (ROS) might play a key role in SSs-associated fibrosis and vascular damage by mediating and/or activating TGF-β-induced EndMT, a phenomenon that has been observed in other disease models. In this review, we identified and critically appraised published studies investigating associations ROS and EndMT and the presence of EndMT in SSc, highlighting a potential link between oxidative stress and EndMT in this condition.
DOI : 10.3389/fimmu.2018.01985
Anahtar Kelimeler : Endothelial-to-Mesenchymal Transition, oxidative stress, reactive oxygen species, scleroderma, systemic sclerosis
ISSN: 1664-3224
Cilt: 9
Sınıflandırma :
Biyotıp %100
9
Human Serum Albumin Increases the Stability of Green Tea Catechins in Aqueous Physiological Conditions
Araştırma Makalesi

Araştırma Makalesi
Open Access
Kaynak :
PLOS - Public Library of Science
Dergi:
Public Library of Science
Yazar(lar) :
Gianfranco Pintus , Ciriaco Carru , Anna Maria Posadino , Annalisa Cossu , Roberta Giordo , Mauro Forteschi , Bastianina Scanu , Salvatore Sotgia , Angelo Zinellu
Yıl :
2015
Gianfranco Pintus
Yayıncı Web Sitesi
Tam Metin
Kaydet Alıntıla
Epicatechin (EC), epigallocatechin (EGC), epicatechingallate (ECG) and epigallocatechingallate (EGCG) are antioxidants present in the green tea, a widely used beverage whose health benefits are largely recognized. Nevertheless, major physicochemical limitations, such as the high instability of catechins, pose important questions concerning their potential pharmacological use. Recent studies indicate that binding of catechins with plasmatic proteins may modulate their plasma concentration, tissue delivery and biological activity. After 5 minutes of incubation with HSA both ECG and EGCG were fully bound to HSA, while after 48h incubation only 41% of EC and 70% of EGC resulted linked. HSA had a strong stabilizing effect on all catechins, which could be found in solution between 29 and 85% even after 48h of incubation. In the absence of HSA, EGC and EGCG disappeared in less than 24h, while ECG and EC were found after 48h at 5 and 50%, respectively. The stabilizing effect of HSA toward EGCG, obtained in aqueous physiological conditions, resulted stronger in comparison to cysteine and HCl, previously reported to stabilize this polyphenol. Because of the multitude of contradictory data concerning in vivo and in vitro antioxidant-based experimentations, we believe our work may shed some light on this debated field of research.
DOI : 10.1371/journal.pone.0134690
Anahtar Kelimeler : Electrocardiography, Cysteine, Serum albumin, Albumins, Capillary electrophoresis, Tea, Antioxidants, Blood plasma
Sınıflandırma :
10
Increased Aortic Calpain-1 Activity Mediates Age-Associated Angiotensin II Signaling of Vascular Smooth Muscle Cells
Araştırma Makalesi

Araştırma Makalesi
Open Access
Kaynak :
PLOS - Public Library of Science
Dergi:
Public Library of Science
Yazar(lar) :
Edward G. Lakatta , Gianfranco Pintus , Gaia Spinetti , Richard Telljohann , Robert E. Monticone , Jing Zhang , Mingyi Wang , Liqun Jiang
Yıl :
2008
Gianfranco Pintus
Yayıncı Web Sitesi
Tam Metin
Kaydet Alıntıla
BackgroundAngiotensin II (Ang II) signaling, including matrix metalloproteinase type II (MMP2) activation, has been linked to an age-associated increase in migration capacity of vascular smooth muscle cells (VSMC), and to other proinflammatory features of arterial aging. Calpain-1 activation is required for MMP2 expression in fibroblasts and is induced in cardiomyocytes by Ang II. The consequences of engagement of calpain-1 with its substrates, however, in governing the age-associated proinflammatory status within the arterial wall, remains unknown.Methodology/Principal FindingsThe present findings demonstrate that transcription, translation, and activity of calpain-1 are significantly up-regulated in rat aortae or early-passage aortic VSMC from old (30-mo) rats compared to young (8-mo). Dual immunolabeling of the arterial wall indicates that colocalization of calpain-1 and Ang II increases within the aged arterial wall. To further explore the relationship of calpain-1 to Ang II, we chronically infused Ang II into young rats, and treated cultured aortic rings or VSMC with Ang II. We also constructed adenoviruses harboring calpain-1 (CANP1) or its endogenous inhibitor calpastatin (CAST) and infected these into VSMC. Ang II induces calpain-1 expression in the aortic walls in vivo and ex vivo and VSMC in vitro. The Ang II mediated, age-associated increased MMP2 activity and migration in VSMC are both blocked by calpain inhibitor 1 or CAST. Over-expression of calpain-1 in young VSMC results in cleavage of intact vimentin, and an increased migratory capacity mimicking that of old VSMC, which is blocked by the MMP inhibitor, GM6001.Conclusions/SignificanceCalpain-1 activation is a pivotal molecular event in the age-associated arterial Ang II/MMP2 signaling cascade that is linked to cytoskeleton protein restructuring, and VSMC migration. Therefore, targeting calpain-1 has the potential to delay or reverse the arterial remodeling that underlies age-associated diseases i.e. atherosclerosis.
DOI : 10.1371/journal.pone.0002231
Anahtar Kelimeler : Spectrins, Vimentin, Adenoviruses, Aging, Calpain inhibitors, Polymerase chain reaction, Fibroblasts, Cytoskeleton
Sınıflandırma :
11
Flavonoids in hypertension: a brief review of the underlying mechanisms
Dergi Makalesi

Dergi Makalesi
Kaynak :
Science Direct
Dergi:
Current Opinion in Pharmacology
Yayıncı:
Elsevier
Yazar(lar) :
Dina Maaliki , Abdullah A Shaito , Gianfranco Pintus , Ahmed El-Yazbi , Ali H Eid
Yıl :
2019
Gianfranco Pintus
Yayıncı Web Sitesi
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Kaydet Alıntıla
Flavonoids are a diverse group of bioactive polyphenolic compounds abundant in dietary plants and herbs. Regular consumption of flavonoids exerts cardio-vasculoprotective effects and may reduce the onset or progression of many cardiovascular diseases, particularly hypertension. Observational studies suggest inverse associations among either of these three combinations: a) anthocyanin intake and risk of myocardial infarction (MI), b) flavanone intake and risk of ischemic stroke and c) flavonol intake and risk of type 2 diabetes mellitus. Human randomized controlled trials (RCTs) show that catechins and quercetin impart significant blood pressure lowering effects. Mechanistically, flavonoids mediate their antihypertensive effects through increasing nitric oxide (NO) bioavailability, reducing endothelial cell oxidative stress or modulating vascular ion channel activity. In this review, we focus on the six main subgroups of flavonoids, namely flavones, flavonols, flavanols, flavanones, anthocyanins, and isoflavones. We further discuss their antihypertensive effects, and their possible mechanisms of regulating blood pressure. We conclude by addressing the safety of these compounds as well as their potential use in hypertension management and treatment.
DOI : 10.1016/j.coph.2019.04.014
ISSN: 1471-4892
Cilt: 45
Sayfa: 57-65
Sınıflandırma :
12
MicroRNAs in Cardiac Hypertrophy
Dergi Makalesi

Dergi Makalesi
Open Access
Kaynak :
Directory of Open Access Journals (DOAJ)
Dergi:
International Journal of Molecular Sciences
Yazar(lar) :
Nadine Wehbe , Suzanne Awani Nasser , Gianfranco Pintus , Adnan Badran , Ali H. Eid , Elias Baydoun
Yıl :
2019
Gianfranco Pintus
Piri Tam Metin Bulucu
Kaydet Alıntıla
Like other organs, the heart undergoes normal adaptive remodeling, such as cardiac hypertrophy, with age. This remodeling, however, is intensified under stress and pathological conditions. Cardiac remodeling could be beneficial for a short period of time, to maintain a normal cardiac output in times of need; however, chronic cardiac hypertrophy may lead to heart failure and death. MicroRNAs (miRNAs) are known to have a role in the regulation of cardiac hypertrophy. This paper reviews recent advances in the field of miRNAs and cardiac hypertrophy, highlighting the latest findings for targeted genes and involved signaling pathways. By targeting pro-hypertrophic genes and signaling pathways, some of these miRNAs alleviate cardiac hypertrophy, while others enhance it. Therefore, miRNAs represent very promising potential pharmacotherapeutic targets for the management and treatment of cardiac hypertrophy.
DOI : 10.3390/ijms20194714
Anahtar Kelimeler : cardiac remodeling, cardiac hypertrophy, MicroRNAs, therapeutic targets, cardiomyocyte
Cilt: 20
Sayı: 19
Sayfa: 4714
Sınıflandırma :
13
Epstein-Barr Virus Epidemiology, Serology, and Genetic Variability of LMP-1 Oncogene Among Healthy Population: An Update.
Dergi Makalesi

Dergi Makalesi
Open Access
Kaynak :
PubMed
Dergi:
Front Oncol
Yazar(lar) :
Maria K Smatti , Duaa W Al-Sadeq , Nadima H Ali , Gianfranco Pintus , Haissam Abou-Saleh , Gheyath K Nasrallah
Yıl :
2018
Gianfranco Pintus
Yayıncı Web Sitesi
 Piri Tam Metin Bulucu
Kaydet Alıntıla
The Epstein-Barr virus (EBV) is a DNA lymphotropic herpesvirus and the causative agent of infectious mononucleosis. EBV is highly prevalent since it affects more than 90% of individuals worldwide and has been linked to several malignancies including PTLDs, which are one of the most common malignancies following transplantation. Among all the EBV genes, most of the recent investigations focused on studying the LMP-1 oncogene because of its high degree of polymorphism and association with tumorigenic activity. There are two main EBV genotypes, Type 1 and 2, distinguished by the differences in the EBNA-2 gene. Further sub genotyping can be characterized by analyzing the LMP-1 gene variation. The virus primarily transmits through oral secretions and persists as a latent infection in human B-cells. However, it can be transmitted through organ transplantations and blood transfusions. In addition, symptoms of EBV infection are not distinguishable from other viral infections, and therefore, it remains questionable whether there is a need to screen for EBV prior to blood transfusion. Although the process of leukoreduction decreases the viral copies present in the leukocytes, it does not eliminate the risk of EBV transmission through blood products. Here, we provide a review of the EBV epidemiology and the genetic variability of the oncogene LMP-1. Then, we underscore the findings of recent EBV seroprevalence and viremia studies among blood donors as a highly prevalent transfusion transmissible oncovirus.
DOI : 10.3389/fonc.2018.00211
Anahtar Kelimeler : Epsteinu2013Barr virus, LMP-1 oncogene, blood donors, seroprevalence, transfusion, viremia
Sınıflandırma :
14
Herbal Medicine for Cardiovascular Diseases: Efficacy, Mechanisms, and Safety
Dergi Makalesi

Dergi Makalesi
Open Access
Kaynak :
Directory of Open Access Journals (DOAJ)
Dergi:
Frontiers in Pharmacology
Yazar(lar) :
Abdullah Shaito , Duong Thi Bich Thuan , Hoa Thi Phu , Thi Hieu Dung Nguyen , Hiba Hasan , Sarah Halabi , Samar Abdelhady , Gheyath K. Nasrallah , Ali H. Eid , Ali H. Eid , Gianfranco Pintus , Gianfranco Pintus
Yıl :
2020
Gianfranco Pintus Gianfranco Pintus
Yayıncı Web Sitesi
 Piri Tam Metin Bulucu
Kaydet Alıntıla
Cardiovascular diseases (CVDs) are a significant health burden with an ever-increasing prevalence. They remain the leading causes of morbidity and mortality worldwide. The use of medicinal herbs continues to be an alternative treatment approach for several diseases including CVDs. Currently, there is an unprecedented drive for the use of herbal preparations in modern medicinal systems. This drive is powered by several aspects, prime among which are their cost-effective therapeutic promise compared to standard modern therapies and the general belief that they are safe. Nonetheless, the claimed safety of herbal preparations yet remains to be properly tested. Consequently, public awareness should be raised regarding medicinal herbs safety, toxicity, potentially life-threatening adverse effects, and possible herb–drug interactions. Over the years, laboratory data have shown that medicinal herbs may have therapeutic value in CVDs as they can interfere with several CVD risk factors. Accordingly, there have been many attempts to move studies on medicinal herbs from the bench to the bedside, in order to effectively employ herbs in CVD treatments. In this review, we introduce CVDs and their risk factors. Then we overview the use of herbs for disease treatment in general and CVDs in particular. Further, data on the ethnopharmacological therapeutic potentials and medicinal properties against CVDs of four widely used plants, namely Ginseng, Ginkgo biloba, Ganoderma lucidum, and Gynostemma pentaphyllum, are gathered and reviewed. In particular, the employment of these four plants in the context of CVDs, such as myocardial infarction, hypertension, peripheral vascular diseases, coronary heart disease, cardiomyopathies, and dyslipidemias has been reviewed, analyzed, and critically discussed. We also endeavor to document the recent studies aimed to dissect the cellular and molecular cardio-protective mechanisms of the four plants, using recently reported in vitro and in vivo studies. Finally, we reviewed and reported the results of the recent clinical trials that have been conducted using these four medicinal herbs with special emphasis on their efficacy, safety, and toxicity.
DOI : 10.3389/fphar.2020.00422
Anahtar Kelimeler : herbal medicine, cardiovascular diseases, atherosclerosis, hypertension, medicinal plants, antioxidants
ISSN: 1663-9812
Cilt: 11
Sınıflandırma :
15
Ultra-Performance Liquid Chromatographic Determination of L-Ergothioneine in Commercially Available Classes of Cow Milk
Dergi Makalesi

Dergi Makalesi
Kaynak :
Wiley Online Library
Dergi:
Journal of Food Science
Yazar(lar) :
Salvatore Sotgia , Elisabetta Pisanu , Debora Cambedda , Gianfranco Pintus , Ciriaco Carru , Angelo Zinellu
Yıl :
2014
Gianfranco Pintus
Yayıncı Web Sitesi
Tam Metin
Kaydet Alıntıla
A new efficient and sensitive precolumn hydrophilic interaction ultra‐performance liquid chromatography (HILIC‐UPLC) method was established for the quantitative determination of L‐ergothioneine (ERT) in milk. After derivatization of ERT with 7‐diethylamino‐3‐[4‐(iodoacetamido)phenyl]‐4‐methylcoumarin, chromatographic separation was achieved in a fairly short time, less than 5 min, on a 100 × 2.1 mm Waters Cortecs UPLC HILIC 1.6‐μm column, by using a mixture of 30 mmol/L ammonium acetate/acetonitrile (10:90, v/v) as a mobile phase flowing isocratically at 0.9 mL/min. Limit of detection and the limit of quantification were 0.03 and 0.10 μmol/L, respectively. The method exhibited linearity in a concentration range of 0.16 and 5.08 μmol/L. Mean recovery was 106.66%, whereas intra‐ and interassay precisions were determined to be within 6 RSD%. On average, ERT concentration in different commercially available classes of cow milk was found to be 0.442 ± 0.191 μmol/L, with the highest levels in the ultra‐high temperature milks and low values in the unprocessed and HTST whole milks. In this light, our experiments suggest that ERT could be used as a marker for the heat treatment of milk.
DOI : 10.1111/1750-3841.12564
ISSN: 0022-1147
Sayı: 9
Cilt: 79
Sayfa: C1683-C1687
16
Nano-targeting vascular remodeling in cancer: Recent developments and future directions
Dergi Makalesi

Dergi Makalesi
Kaynak :
Science Direct
Dergi:
Seminars in Cancer Biology
Yayıncı:
Elsevier BV
Yazar(lar) :
Roberta Giordo , Zena Wehbe , Panagiotis Paliogiannis , Ali H. Eid , Arduino A. Mangoni , Gianfranco Pintus
Yıl :
2022
Gianfranco Pintus
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Kaydet Alıntıla
DOI : 10.1016/j.semcancer.2022.03.001
ISSN: 1044-579X
17
Improved method for plasma ADMA, SDMA, and arginine quantification by field-amplified sample injection capillary electrophoresis UV detection
Dergi Makalesi

Dergi Makalesi
Kaynak :
Springer Link
Dergi:
Analytical and Bioanalytical Chemistry
Yazar(lar) :
Angelo Zinellu , Salvatore Sotgia , Maria Franca Usai , Gianfranco Pintus , Luca Deiana , Ciriaco Carru
Konu :
Biochemistry, Analytical Chemistry
Yıl :
2010
Gianfranco Pintus
Yayıncı Web Sitesi
Tam Metin
Kaydet Alıntıla
Here, we describe an easy field-amplified sample injection capillary electrophoresis method with UV detection for the separation and detection of free plasma arginine and dimethylated arginines. The analytes were baseline-separated within 22 min by using 50 mmol/L Tris phosphate pH 2.3 as running buffer. The plasma samples were treated with acetonitrile/ammonia for protein elimination, the supernatants were dried, re-swollen in water and directly injected in the capillary without complex cleanup by solid phase extraction and/or tedious sample derivatization procedures. Due to the stacking effects of the electrokinetic injection, it was possible to operate a consistent on-line pre-concentration of the analytes before running the electrophoresis. This procedure allowed to reach a detection limit in the real sample of 10 nmol/L for dimethylated arginines and 20 nmol/L for arginine, thus improving about threefold our previous method, that required a more complicated pre-analytical procedure to concentrate samples. The recovery of plasma ADMA was 99–104% and inter-day CV was less than 3%. The assay performance was evaluated measuring the levels of arginine and its dimethyl derivatives in 50 subjects. The statistical tests for the methods comparison suggest that the data obtained by our new method and by our previous CE assay are similar.
DOI : 10.1007/s00216-010-4580-0
Anahtar Kelimeler : ADMA, SDMA, Capillary electrophoresis, FASI
ISSN: 1618-2642 1618-2650
Sayı: 5
Cilt: 399
Sayfa: 1815-1821
18
Nox2 Activity Is Required in Obesity-Mediated Alteration of Bone Remodeling
Dergi Makalesi

Dergi Makalesi
Open Access
Kaynak :
Directory of Open Access Journals (DOAJ)
Dergi:
Oxidative Medicine and Cellular Longevity
Yazar(lar) :
Md Mizanur Rahman , Amina El Jamali , Ganesh V. Halade , Allal Ouhtit , Haissam Abou-Saleh , Gianfranco Pintus
Yıl :
2018
Gianfranco Pintus
Piri Tam Metin Bulucu
Kaydet Alıntıla
Despite increasing evidence suggesting a role for NADPH oxidases (Nox) in bone pathophysiology, whether Nox enzymes contribute to obesity-mediated bone remodeling remains to be clearly elucidated. Nox2 is one of the predominant Nox enzymes expressed in the bone marrow microenvironment and is a major source of ROS generation during inflammatory processes. It is also well recognized that a high-fat diet (HFD) induces obesity, which negatively impacts bone remodeling. In this work, we investigated the effect of Nox2 loss of function on obesity-mediated alteration of bone remodeling using wild-type (WT) and Nox2-knockout (KO) mice fed with a standard lab chow diet (SD) as a control or a HFD as an obesity model. Bone mineral density (BMD) of mice was assessed at the beginning and after 3 months of feeding with SD or HFD. Our results show that HFD increased bone mineral density to a greater extent in KO mice than in WT mice without affecting the total body weight and fat mass. HFD also significantly increased the number of adipocytes in the bone marrow microenvironment of WT mice as compared to KO mice. The bone levels of proinflammatory cytokines and proosteoclastogenic factors were also significantly elevated in WT-HFD mice as compared to KO-HFD mice. Furthermore, the in vitro differentiation of bone marrow cells into osteoclasts was significantly increased when using bone marrow cells from WT-HFD mice as compared to KO-HFD mice. Our data collectively suggest that Nox2 is implicated in HFD-induced deleterious bone remodeling by enhancing bone marrow adipogenesis and osteoclastogenesis.
DOI : 10.1155/2018/6054361
Cilt: 2018
Sınıflandırma :
19
Heparin down-regulates the phorbol ester-induced protein kinase C gene expression in human endothelial cells: enzyme-mediated autoregulation of protein kinase C-α and -δ genes1
Dergi Makalesi

Dergi Makalesi
Kaynak :
Wiley Online Library
Dergi:
FEBS Letters
Yazar(lar) :
Gianfranco Pintus , Bruna Tadolini , Margherita Maioli , Anna M. Posadino , Leonardo Gaspa , Carlo Ventura
Yıl :
1999
Gianfranco Pintus
Yayıncı Web Sitesi
Tam Metin
Kaydet Alıntıla
Overexpression of protein kinase C‐α and protein kinase C‐δ has been shown to modulate a number of biological effects, including the cell growth and differentiation. We hypothesized that heparin, a potent antimitogenic drug, could affect the cell proliferation by inhibiting the expression of specific protein kinase C genes. Heparin, markedly but not completely, inhibited the serum‐stimulated protein kinase C‐α and ‐δ mRNA expression. Protein kinase C inhibition or down‐regulation significantly decreased the serum‐induced protein kinase C isoenzyme gene expression. Heparin failed to inhibit the residual effect of serum that was resistant to the above‐mentioned treatments. Phorbol 12‐myristate 13‐acetate elicited an increase of protein kinase C isoenzyme gene expression that was completely prevented by protein kinase C inhibition or down‐regulation. Heparin dose‐dependently counteracted and ultimately abolished the increase in the protein kinase C isoenzyme gene expression elicited by phorbol 12‐myristate 13‐acetate. These results suggest that the inhibition of an autoregulatory role wielded by protein kinase C on the protein kinase C‐α and ‐δ gene expression might represent a possible mechanism by which glycosaminoglycans modulate the cell growth.
DOI : 10.1016/s0014-5793(99)00438-x
ISSN: 0014-5793
Sayı: 2-3
Cilt: 449
Sayfa: 135-140
20
Evaluation of Global Genomic DNA Methylation in Human Whole Blood by Capillary Electrophoresis UV Detection
Dergi Makalesi

Dergi Makalesi
Open Access
Kaynak :
Directory of Open Access Journals (DOAJ)
Dergi:
Journal of Analytical Methods in Chemistry
Yazar(lar) :
Angelo Zinellu , Elisabetta Sotgiu , Stefano Assaretti , Salvatore Sotgia , Panagiotis Paliogiannis , Gianfranco Pintus , Arduino A. Mangoni , Ciriaco Carru
Yıl :
2017
Gianfranco Pintus
Yayıncı Web Sitesi
 Piri Tam Metin Bulucu
Kaydet Alıntıla
Alterations in global DNA methylation are implicated in various pathophysiological processes. The development of simple and quick, yet robust, methods to assess DNA methylation is required to facilitate its measurement and interpretation in clinical practice. We describe a highly sensitive and reproducible capillary electrophoresis method with UV detection for the separation and detection of cytosine and methylcytosine, after formic acid hydrolysis of DNA extracted from human whole blood. Hydrolysed samples were dried and resuspended with water and directly injected into the capillary without sample derivatization procedures. The use of a run buffer containing 50 mmol/L BIS-TRIS propane (BTP) phosphate buffer at pH 3.25 and 60 mmol/L sodium acetate buffer at pH 3.60 (4 : 1, v/v) allowed full analyte identification within 11 min. Precision tests indicated an elevated reproducibility with an interassay CV of 1.98% when starting from 2 μg of the extracted DNA. The method was successfully tested by measuring the DNA methylation degree both in healthy volunteers and in reference calf thymus DNA.
DOI : 10.1155/2017/4065892
Cilt: 2017
Sınıflandırma :
21
Quantification of L-ergothioneine in whole blood by hydrophilic interaction ultra-performance liquid chromatography and UV-detection
Dergi Makalesi

Dergi Makalesi
Kaynak :
Wiley Online Library
Dergi:
Journal of Separation Science
Yazar(lar) :
Salvatore Sotgia , Angelo Zinellu , Gianfranco Pintus , Gerard Aime Pinna , Luca Deiana , Ciriaco Carru
Yıl :
2013
Gianfranco Pintus
Yayıncı Web Sitesi
Tam Metin
Kaydet Alıntıla
A new hydrophilic interaction ultra‐performance LC method was established for the whole blood measurement of L‐ergothioneine. Chromatographic separation was achieved in a fairly short time, less than 4 min, on a 100 × 2.1 mm Acquity UPLC BEH HILIC 1.7 μm column with a mobile phase consisting of a mixture of 100 mmol/L ammonium acetate/ACN/water (5:85:10, v/v/v) that flowed isocratically at 0.250 mL/min. The LOD and the limit of quantification were 3.85 and 11.67 μmol/L, respectively. The method exhibited linearity in a concentration range of 15.63–1000 μmol/L (R2 > 0.999). Mean recovery was 96.34% whereas intraassay and interassay precision were 1.52 and 1.82% RSD, respectively. On the whole, the developed method is simple, fast, precise, accurate, and sensitive and may be useful for routine analyses.
DOI : 10.1002/jssc.201201016
ISSN: 1615-9306
Sayı: 6
Cilt: 36
Sayfa: 1002-1006
22
Nose-to-brain delivery of BACE1 siRNA loaded in solid lipid nanoparticles for Alzheimer’s therapy
Dergi Makalesi

Dergi Makalesi
Kaynak :
Science Direct
Dergi:
Colloids and Surfaces B: Biointerfaces
Yayıncı:
Elsevier
Yazar(lar) :
Giovanna Rassu , Elena Soddu , Anna Maria Posadino , Gianfranco Pintus , Bruno Sarmento , Paolo Giunchedi , Elisabetta Gavini
Yıl :
2017
Gianfranco Pintus
Yayıncı Web Sitesi
Tam Metin
Kaydet Alıntıla
We designed a delivery system to obtain an efficient and optimal nose-to-brain transport of BACE1 siRNA, potentially useful in the treatment of Alzheimer's disease. We selected a cell-penetrating peptide, the short peptide derived from rabies virus glycoprotein known as RVG-9R, to increase the transcellular pathway in neuronal cells. The optimal molar ratio between RVG-9R and BACE1 siRNA was elucidated. The complex between the two was then encapsulated. We propose chitosan-coated and uncoated solid lipid nanoparticles (SLNs) as a nasal delivery system capable of exploiting both olfactory and trigeminal nerve pathways. The coating process had an effect on the zeta potential, obtaining positively-charged nanoparticles, and on siRNA protection. The positive charge of the coating formulation ensured mucoadhesiveness to the particles and also prolonged residence time in the nasal cavity. We studied the cellular transport of siRNA released from the SLNs using Caco-2 as a model of epithelial-like phenotypes. We found that siRNA permeates the monolayer to a greater extent when released from any of the studied formulations than from bare siRNA, and primarily from chitosan-coated SLNs.
DOI : 10.1016/j.colsurfb.2017.01.031
Anahtar Kelimeler : BACE1 β-secretase responsible for amyloid-β (Aβ) generation in the brain, β-secretase responsible for amyloid-β (Aβ) generation in the brain, BBB blood−brain barrier, blood−brain barrier, Caco-2 human epithelial colorectal adenocarcinoma cells, human epithelial colorectal adenocarcinoma cells, C chitosan coated nanoparticles, chitosan coated nanoparticles, CNS central nervous system, central nervous system, CPPs cell-penetrating peptides, cell-penetrating peptides, CS low molecular weight chitosan, low molecular weight chitosan, CSF cerebrospinal fluid, cerebrospinal fluid, 6-FAM 6-carboxyfluorescein, 6-carboxyfluorescein, NR uncoated nanoparticles, uncoated nanoparticles, OB olfactory bulb, olfactory bulb, PVA poly(vinyl alcohol), poly(vinyl alcohol), RVG-9R Chimeric Rabies Virus Glycoprotein fragment, Chimeric Rabies Virus Glycoprotein fragment, SLN solid lipid nanoparticles, solid lipid nanoparticles, Nose-to-brain transport, siRNA, Solid lipid nanoparticle, Chitosan, RVG-9R cell-penetrating peptide
ISSN: 0927-7765
Cilt: 152
Sayfa: 296-301
Sınıflandırma :
23
Plasma L-ergothioneine measurement by high-performance liquid chromatography and capillary electrophoresis after a pre-column derivatization with 5-iodoacetamidofluorescein (5-IAF) and fluorescence detection.
Dergi Makalesi

Dergi Makalesi
Open Access
Kaynak :
Directory of Open Access Journals (DOAJ)
Dergi:
PLoS ONE
Yazar(lar) :
Salvatore Sotgia , Elisabetta Pisanu , Gianfranco Pintus , Gian Luca Erre , Gerard Aime Pinna , Luca Deiana , Ciriaco Carru , Angelo Zinellu
Yıl :
2013
Gianfranco Pintus
Yayıncı Web Sitesi
Tam Metin
Kaydet Alıntıla
Two sensitive and reproducible capillary electrophoresis and high-performance liquid chromatography-fluorescence procedures were established for quantitative determination of L-egothioneine in plasma. After derivatization of L-ergothioneine with 5-iodoacetamidofluorescein, the separation was carried out by HPLC on an ODS-2 C-18 sperisorb column by using a linear gradient elution and by HPCE on an uncoated fused silica capillary, 50 µm id, and 60 cm length. The methods were validated and found to be linear in the range of 0.3 to 10 µmol/l. The limit of quantification was 0.27 µmol/l for HPCE and 0.15 µmol/l for HPLC. The variations for intra- and inter-assay precision were around 6 RSD%, and the mean recovery accuracy close to 100% (96.11%).
DOI : 10.1371/journal.pone.0070374
ISSN: 1932-6203
Cilt: 8
Sayı: 7
Sayfa: e70374
Sınıflandırma :
Kimya %100
Kromatografi %100

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