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Nicotinamide-Rich Diet in DBA/2J Mice Preserves Retinal Ganglion Cell Metabolic Function as Assessed by PERG Adaptation to Flicker
Dergi Makalesi

Dergi Makalesi
Open Access
Kaynak 1:
Directory of Open Access Journals (DOAJ)
Kaynak 2:
MDPI
Dergi:
Nutrients
Yazar(lar) :
Tsung-Han Chou , Giovanni Luca Romano , Rosario Amato , Vittorio Porciatti
Yıl :
2020
Giovanni Luca Romano
Piri Tam Metin Bulucu
Kaydet Alıntıla
Flickering light increases metabolic demand in the inner retina. Flicker may exacerbate defective mitochondrial function in glaucoma, which will be reflected in the pattern electroretinogram (PERG), a sensitive test of retinal ganglion cell (RGC) function. We tested whether flicker altered the PERG of DBA/2J (D2) glaucomatous mice and whether vitamin B3-rich diet contributed to the flicker effect. D2 mice fed with either standard chow (control, n = 10) or chow/water enriched with nicotinamide (NAM, 2000 mg/kg per day) (treated, n = 10) were monitored from 3 to 12 months. The PERG was recorded with superimposed flicker (F-PERG) at either 101 Hz (baseline) or 11 Hz (test), and baseline-test amplitude difference (adaptation) evaluated. At endpoint, flat-mounted retinas were immunostained (RBPMS and mito-tracker). F-PERG adaptation was 41% in 3-month-old D2 and decreased with age more in control D2 than in NAM-fed D2 (GEE, p < 0.01). At the endpoint, F-PERG adaptation was 0% in control D2 and 17.5% in NAM-fed D2, together with higher RGC density (2.4×), larger RGC soma size (2×), and greater intensity of mitochondrial staining (3.75×). F-PERG adaptation may provide a non-invasive tool to assess RGC autoregulation in response to increased metabolic demand and test the effect of dietary/pharmacological treatments on optic nerve disorders.
DOI : 10.3390/nu12071910
Anahtar Kelimeler : adaptation, retinal ganglion cell, pattern electroretinogram, nicotinamide, mitochondria
ISSN: 2072-6643
Cilt: 12
Sayı: 1910
Sayfa: 1910
2
Anesthetic Preconditioning as Endogenous Neuroprotection in Glaucoma
Dergi Makalesi

Dergi Makalesi
Open Access
Kaynak :
Directory of Open Access Journals (DOAJ)
Dergi:
International Journal of Molecular Sciences
Yazar(lar) :
Tsung-Han Chou , Ganeswara Rao Musada , Giovanni Luca Romano , Elizabeth Bolton , Vittorio Porciatti
Yıl :
2018
Giovanni Luca Romano
Yayıncı Web Sitesi
 Piri Tam Metin Bulucu
Kaydet Alıntıla
Blindness in glaucoma is the result of death of Retinal Ganglion Cells (RGCs) and their axons. RGC death is generally preceded by a stage of reversible dysfunction and structural remodeling. Current treatments aimed at reducing intraocular pressure (IOP) are ineffective or incompletely effective in management of the disease. IOP-independent neuroprotection or neuroprotection as adjuvant to IOP lowering in glaucoma remains a challenge as effective agents without side effects have not been identified yet. We show in DBA/2J mice with spontaneous IOP elevation and glaucoma that the lifespan of functional RGCs can be extended by preconditioning RGCs with retrobulbar lidocaine in one eye at four months of age that temporary blocks RGC axonal transport. The contralateral, PBS-injected eye served as control. Lidocaine-induced impairment of axonal transport to superior colliculi was assessed by intravitreal injection of cholera toxin B. Long-term (nine months) effect of lidocaine were assessed on RGC electrical responsiveness (PERG), IOP, expression of relevant protein (BDNF, TrkB, PSD95, GFAP, Synaptophysin, and GAPDH) and RGC density. While lidocaine treatment did not alter the age-related increase of IOP, TrkB expression was elevated, GFAP expression was decreased, RGC survival was improved by 35%, and PERG function was preserved. Results suggest that the lifespan of functional RGCs in mouse glaucoma can be extended by preconditioning RGCs in early stages of the disease using a minimally invasive treatment with retrobulbar lidocaine, a common ophthalmologic procedure. Lidocaine is inexpensive, safe and is approved by Food and Drug Administration (FDA) to be administered intravenously.
DOI : 10.3390/ijms19010237
Anahtar Kelimeler : glaucoma, mouse, retinal ganglion cells, lidocaine, neuroprotection
Cilt: 19
Sayı: 1
Sayfa: 237
Sınıflandırma :
Biyotıp %100
Biyotıp %88
3
P2X7 receptor antagonism preserves retinal ganglion cells in glaucomatous mice
Dergi Makalesi

Dergi Makalesi
Kaynak :
Science Direct
Dergi:
Biochemical Pharmacology
Yayıncı:
Elsevier
Yazar(lar) :
Giovanni Luca Romano , Rosario Amato , Francesca Lazzara , Vittorio Porciatti , Tsung-Han Chou , Filippo Drago , Claudio Bucolo
Yıl :
2020
Giovanni Luca Romano
Yayıncı Web Sitesi
Tam Metin
Kaydet Alıntıla
To investigate the role of P2X7 receptor to preserve retinal ganglion cells (RGCs) structure and function in a genetic mouse model (DBA/2J mouse) of age-related glaucomatous neurodegeneration. Chronic treatment with P2X7 receptor antagonist eye drops was carried out in order to assess RGCs function and density by pattern electroretinogram (PERG) and RBPMS immunostaining, respectively. Further, microglia activation was assessed in flat-mounted retina by using Iba-1 immunostaining. Untreated glaucomatous eyes displayed significant microglia activation, alteration of PERG signal, and RGCs loss. In the P2X7 receptor antagonist-treated eyes, the PERG signal was significantly (p < 0.05) improved compared to controls, along with a significant (p < 0.05) reduction in terms of retinal microglial activation, and remarkable preservation of RGCs density. Altogether, these findings demonstrated that topical treatment with a P2X7 receptor antagonist has a neuroprotective effect on RGCs in glaucomatous mice, suggesting an appealing pharmacological approach to prevent retinal degenerative damage in optic neuropathy.
DOI : 10.1016/j.bcp.2020.114199
Anahtar Kelimeler : P2X7 receptor, Retinal ganglion cells, Glaucoma, PERG
ISSN: 0006-2952
Cilt: 180
Sayfa: 114199
Sınıflandırma :
4
Curcumin prevents high glucose damage in retinal pigment epithelial cells through ERK1/2‐mediated activation of the Nrf2/HO‐1 pathway
Dergi Makalesi

Dergi Makalesi
Kaynak :
Wiley Online Library
Dergi:
Journal of Cellular Physiology
Yazar(lar) :
Claudio Bucolo , Filippo Drago , Rosa Maisto , Giovanni Luca Romano , Velia D'Agata , Grazia Maugeri , Salvatore Giunta
Konu :
Clinical Biochemistry, Cell Biology, Physiology
Yıl :
2019
Giovanni Luca Romano
Yayıncı Web Sitesi
Tam Metin
Kaydet Alıntıla
To study the effects of curcumin on human retinal pigment epithelial (RPE) cells exposed to high glucose (HG) insult, we performed in vitro studies on RPE cells cultured both in normal and HG conditions to assess the effects of curcumin on the cell viability, nuclear factor erythroid 2‐related factor 2 (Nrf2) expression, HO‐1 activity, and ERK1/2 expression. RPE cells exposed to HG insult were treated with curcumin. The cell viability, apoptosis, HO‐1 activity, ERK, and Nrf2 expression were evaluated. The data indicated that treatment with curcumin caused a significant decrease in terms of apoptosis. Further, curcumin was able to induce HO‐1 expression via Nrf2 activation and counteracts the damage elicited by HG. The present study demonstrated that curcumin provides protection against HG‐induced damage in RPE cells through the activation of Nrf2/HO‐1 signaling that involves the ERK pathway, suggesting that curcumin may have therapeutic value in the treatment of diabetic retinopathy.
DOI : 10.1002/jcp.28347
ISSN: 0021-9541
Sayı: 10
Cilt: 234
Sayfa: 17295-17304
5
Influence of different surfactants on the technological properties and in vivo ocular tolerability of lipid nanoparticles
Dergi Makalesi

Dergi Makalesi
Kaynak :
Science Direct
Dergi:
International Journal of Pharmaceutics
Yayıncı:
Elsevier
Yazar(lar) :
Antonio Leonardi , Claudio Bucolo , Giovanni Luca Romano , Chiara Bianca Maria Platania , Filippo Drago , Giovanni Puglisi , Rosario Pignatello
Yıl :
2014
Giovanni Luca Romano
Yayıncı Web Sitesi
Tam Metin
Kaydet Alıntıla
Addition of one or more surfactant agents is often necessary for the production of nanostructured lipid and polymeric systems. The removal of residual surfactants is a required step for technological and toxicological reasons, especially for peculiar applications, such as the ophthalmic field. This study was planned to assess the technological properties of some surfactants, commonly used for the production of lipid nanoparticles, as well as their ocular safety profile. Stable and small-size solid lipid nanoparticles were obtained using Dynasan® 114 as the lipid matrix and all the tested surfactants. However, from a toxicological point of view, the nanocarriers produced using Kolliphor® P188 were the most valuable, showing no irritant effect on the ocular surface up to the highest tested surfactant concentration (0.4%, w/v). The SLN produced using Cremophor® A25 and Lipoid® S100 were tolerated up to a surfactant concentration of 0.2% by weight, while for Tween® 80 and Kolliphor® HS 15 a maximum concentration of 0.05% can be considered totally not-irritant.
DOI : 10.1016/j.ijpharm.2014.04.061
Anahtar Kelimeler : Solid lipid nanoparticles, Ocular drug delivery systems, Surfactants, Ocular safety, Eye tolerability
ISSN: 0378-5173
Sayı: 1-2
Cilt: 470
Sayfa: 133-140
Sınıflandırma :
6
Effects of novel hybrids of caffeic acid phenethyl ester and NSAIDs on experimental ocular inflammation
Dergi Makalesi

Dergi Makalesi
Kaynak :
Science Direct
Dergi:
European Journal of Pharmacology
Yayıncı:
Elsevier
Yazar(lar) :
Valeria Pittalà , Loredana Salerno , Giuseppe Romeo , Maria Angela Siracusa , Maria Nunziata Modica , Giovanni Luca Romano , Salvatore Salomone , Filippo Drago , Claudio Bucolo
Yıl :
2015
Giovanni Luca Romano
Yayıncı Web Sitesi
Tam Metin
Kaydet Alıntıla
In this study, we report the design and synthesis of novel hybrids of caffeic acid phenetyl ester (CAPE) and non-steroidal anti-inflammatory drugs (NSAIDs). We assessed their effects on an experimental ocular inflammation in New Zealand rabbits. The formulations of CAPE–aspirin and CAPE–indomethacin hybrids were topical instilled in the rabbit׳s eye. Afterwards, the anti-inflammatory activity was evaluated by grading the clinical signs and by assessing the inflammatory cell count, protein, PGE2 and TNFα levels in the aqueous humor. Furthermore, ocular tolerability of hybrids formulations was evaluated in a separate set of animals by using a modified Draize test. The ocular inflammation in the control group was significantly higher than in both the hybrid-treated groups, as indicated by clinical grading and biomarkers assessment. However, only the CAPE–aspirin hybrid reduced, in a significant dose-dependent manner, the ocular inflammation elicited by paracentesis. CAPE–indomethacin hybrid was able to significantly attenuate the clinical grading and the PGE2 aqueous levels only at the highest dose (0.1%). CAPE–aspirin significantly reduced PGE2 and TNFα levels in the aqueous humor as well as proteins and PMNs. Finally, all formulations showed no ocular irritation compared with vehicle-treated group. In conclusion, CAPE–aspirin shows full anti-inflammatory efficacy in experimental model of ocular inflammation demonstrating an optimal pharmacological and safety profile. Taken together these data indicate that CAPE–aspirin hybrid represents a valid and safe new chemical entity potentially useful for the treatment of ocular inflammation.
DOI : 10.1016/j.ejphar.2015.02.012
Anahtar Kelimeler : Caffeic acid phenethyl ester, Aspirin, Indomethacin, Heme oxygenase-1 inducer, Inflammation, Eye
ISSN: 0014-2999
Cilt: 752
Sayfa: 78-83
Sınıflandırma :
7
TGF-β1 prevents rat retinal insult induced by amyloid-β (1–42) oligomers
Dergi Makalesi

Dergi Makalesi
Kaynak :
Science Direct
Dergi:
European Journal of Pharmacology
Yayıncı:
Elsevier
Yazar(lar) :
Vincenzo Fisichella , Giovanni Giurdanella , Chiara Bianca Maria Platania , Giovanni Luca Romano , Gian Marco Leggio , Salvatore Salomone , Filippo Drago , Filippo Caraci , Claudio Bucolo
Yıl :
2016
Giovanni Luca Romano
Yayıncı Web Sitesi
Tam Metin
Kaydet Alıntıla
To set up a retinal degenerative model in rat that mimics pathologic conditions such as age-related macular degeneration (AMD) using amyloid-β (Aβ) oligomers, and assess the effect of TGF-β1. Sprague-Dawley male rats were used. Human Aβ1–42 oligomers were intravitreally (ITV) injected (10 µM) in the presence or in the absence of recombinant human TGF-β1 (1 ng/μl ITV injected). After 48 h, the animals were sacrificed and the eyes removed and dissected. The apoptotic markers Bax and Bcl-2 were assessed by western blot analysis in retina lysates. Gene-pathway network analysis was carried out in order to identify pathways involved in AMD. Treatment with Aβ oligomers induced a strong increase in Bax protein level (about 4-fold; p<0.01) and a significant reduction in Bcl-2 protein level (about 2-fold; p<0.05). Co-injection of TGF-β1 triggered a significant reduction of Bax protein induced by Aβ oligomers. Bioinformatic analysis revealed that Bcl-2 and PI3K-Akt are the most connected nodes, for genes and pathways respectively, in the enriched gene-pathway network common to AMD and Alzheimer disease (AD). Overall, these data indicate that ITV injection of Aβ1–42 oligomers in rat induces molecular changes associated with apoptosis in rat retina, highlighting a potential pathogenetic role of Aβ oligomers in AMD. Bioinformatics analysis confirms that apoptosis pathways can take part in AMD. Furthermore, these findings suggest that human recombinant TGF-β1 can prevent retinal damage elicited by Aβ oligomers.
DOI : 10.1016/j.ejphar.2016.02.002
Anahtar Kelimeler : Macular degeneration, Retina, Alzheimer's disease, TGF-β1
ISSN: 0014-2999
Cilt: 787
Sayfa: 72-77
Sınıflandırma :
8
COVID-19 Surface Persistence: A Recent Data Summary and Its Importance for Medical and Dental Settings
Dergi Makalesi

Dergi Makalesi
Open Access
Kaynak 1:
Directory of Open Access Journals (DOAJ)
Kaynak 2:
MDPI
Dergi:
International Journal of Environmental Research and Public Health
Yazar(lar) :
Luca Fiorillo , Gabriele Cervino , Marco Matarese , Cesare Du2019Amico , Giovanni Surace , Valeria Paduano , Maria Teresa Fiorillo , Antonio Moschella , Alessia La Bruna , Giovanni Luca Romano , Riccardo Laudicella , Sergio Baldari , Marco Cicciù
Yıl :
2020
Giovanni Luca Romano
Piri Tam Metin Bulucu
Kaydet Alıntıla
Recently, due to the coronavirus pandemic, many guidelines and anti-contagion strategies continue to report unclear information about the persistence of coronavirus disease 2019 (COVID-19) in the environment. This certainly generates insecurity and fear in people, with an important psychological component that is not to be underestimated at this stage of the pandemic. The purpose of this article is to highlight all the sources currently present in the literature concerning the persistence of the different coronaviruses in the environment as well as in medical and dental settings. As this was a current study, there are still not many sources in the literature, and scientific strategies are moving towards therapy and diagnosis, rather than knowing the characteristics of the virus. Such an article could be an aid to summarize virus features and formulate new guidelines and anti-spread strategies.
DOI : 10.3390/ijerph17093132
Anahtar Kelimeler : COVID-19, virus, epidemiology, surfaces, infection risk
Cilt: 17
Sayı: 3132
Sayfa: 3132
Sınıflandırma :
9
Retinal biomarkers and pharmacological targets for Hermansky-Pudlak syndrome 7
Dergi Makalesi

Dergi Makalesi
Open Access
Kaynak :
Directory of Open Access Journals (DOAJ)
Dergi:
Scientific Reports
Yazar(lar) :
Giovanni Luca Romano , Chiara Bianca Maria Platania , Gian Marco Leggio , Sebastiano Alfio Torrisi , Salvatore Giunta , Salvatore Salomone , Michele Purrello , Marco Ragusa , Cristina Barbagallo , Frank J. Giblin , Rosa Mastrogiacomo , Francesca Managò , Maurizio Cammalleri , Francesco Papaleo , Filippo Drago , Claudio Bucolo
Yıl :
2020
Giovanni Luca Romano
Yayıncı Web Sitesi
 Piri Tam Metin Bulucu
Kaydet Alıntıla
Abstract Deletion of dystrobrevin binding protein 1 has been linked to Hermansky-Pudlak syndrome type 7 (HPS-7), a rare disease characterized by oculocutaneous albinism and retinal dysfunction. We studied dysbindin-1 null mutant mice (Dys−/−) to shed light on retinal neurodevelopment defects in HPS-7. We analyzed the expression of a focused set of miRNAs in retina of wild type (WT), Dys+/− and Dys−/− mice. We also investigated the retinal function of these mice through electroretinography (ERG). We found that miR-101-3p, miR-137, miR-186-5p, miR-326, miR-382-5p and miR-876-5p were up-regulated in Dys−/−mice retina. Dys−/− mice showed significant increased b-wave in ERG, compared to WT mice. Bioinformatic analysis highlighted that dysregulated miRNAs target synaptic plasticity and dopaminergic signaling pathways, affecting retinal functions of Dys−/− mice. Overall, the data indicate potential mechanisms in retinal neurodevelopment of Dys−/− mice, which may have translational significance in HSP-7 patients, both in terms of diagnostic/prognostic biomarkers and novel pharmacological targets.
DOI : 10.1038/s41598-020-60931-5
ISSN: 2045-2322
Cilt: 10
Sayı: 1
Sayfa: 1 - 12
Sınıflandırma :
10
Pegylated Asparaginase‐Induced Liver Injury: A Case‐Based Review and Data From Pharmacovigilance
Dergi Makalesi

Dergi Makalesi
Kaynak :
Wiley Online Library
Dergi:
The Journal of Clinical Pharmacology
Yazar(lar) :
Calogero Vetro , Andrea Duminuco , Lucia Gozzo , Cinzia Maugeri , Marina Parisi , Serena Brancati , Laura Longo , Daniela Cristina Vitale , Giovanni Luca Romano , Roberto Ciuni , Elisa Mauro , Paolo Fabio Fiumara , Giuseppe Alberto Maria Palumbo , Filippo Drago , Francesco Di Raimondo
Yıl :
2022
Giovanni Luca Romano
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DOI : 10.1002/jcph.2052
ISSN: 0091-2700

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