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Challenges in Laboratory Diagnosis of the Novel Coronavirus SARS-CoV-2

Dergi Makalesi

Kaynak 1:

Directory of Open Access Journals (DOAJ)

Kaynak 2:

MDPI

Dergi:

Viruses

Yazar(lar) :

Nadin Younes , Duaa W. Al-Sadeq , Hadeel AL-Jighefee , Salma Younes , Ola Al-Jamal , Hanin I. Daas , Hadi. M. Yassine , Gheyath K. Nasrallah

Yıl :

2020

Hadeel AL-Jighefee

Yayıncı Web Siteleri

Piri Tam Metin Bulucu Özet Ek Bilgiler

Kaydet

The recent outbreak of the Coronavirus disease 2019 (COVID-19) has quickly spread worldwide since its discovery in Wuhan city, China in December 2019. A comprehensive strategy, including surveillance, diagnostics, research, clinical treatment, and development of vaccines, is urgently needed to win the battle against COVID-19. The past three unprecedented outbreaks of emerging human coronavirus infections at the beginning of the 21st century have highlighted the importance of readily available, accurate, and rapid diagnostic technologies to contain emerging and re-emerging pandemics. Real-time reverse transcriptase-polymerase chain reaction (rRT-PCR) based assays performed on respiratory specimens remain the gold standard for COVID-19 diagnostics. However, point-of-care technologies and serologic immunoassays are rapidly emerging with high sensitivity and specificity as well. Even though excellent techniques are available for the diagnosis of symptomatic patients with COVID-19 in well-equipped laboratories; critical gaps still remain in screening asymptomatic people who are in the incubation phase of the virus, as well as in the accurate determination of live viral shedding during convalescence to inform decisions for ending isolation. This review article aims to discuss the currently available laboratory methods and surveillance technologies available for the detection of COVID-19, their performance characteristics and highlight the gaps in current diagnostic capacity, and finally, propose potential solutions. We also summarize the specifications of the majority of the available commercial kits (PCR, EIA, and POC) for laboratory diagnosis of COVID-19.

Kaynak 1: https://doi.org/10.3390/v12060582 arayıcılığı görüntüle.
Kaynak 2: http://dx.doi.org/10.3390/v12060582 arayıcılığı görüntüle.

DOI : 10.3390/v12060582

Anahtar Kelimeler : COVID-19, SARS-CoV-2, viruses, diagnostic challenges, molecular testing, serology

ISSN: 1999-4915
Cilt: 12
Sayı: 582
Sayfa: 582

Sınıflandırma :

Tıp ve Halk Sağlığı %87

Bulaşıcı Hastalıklar %93

Organ-specific toxicity evaluation of stearamidopropyl dimethylamine (SAPDMA) surfactant using zebrafish embryos

Dergi Makalesi

Kaynak :

Science Direct

Dergi:

Science of The Total Environment

Yayıncı:

Elsevier

Yazar(lar) :

Ola Al-Jamal , Hadeel Al-Jighefee , Nadin Younes , Roba Abdin , Maha A. Al-Asmakh , A. Bahgat Radwan , Mostafa H. Sliem , Amin F. Majdalawieh , Gianfranco Pintus , Hadi M. Yassine , Aboubakr M. Abdullah , Sahar I. Da'as , Gheyath K. Nasrallah

Yıl :

2020

Hadeel Al-Jighefee

Yayıncı Web Sitesi

Tam Metin

Ek Bilgiler

Kaydet

DOI : 10.1016/j.scitotenv.2020.140450

ISSN: 0048-9697
Cilt: 741
Sayfa: 140450

Sınıflandırma :

Çevre %71

Kirlilik %74

Kimya %25

Fiziksel kimya %50

Tıbbi Kimya %39

Analytic comparison between three high-throughput commercial SARS-CoV-2 antibody assays reveals minor discrepancies in a high-incidence population

Dergi Makalesi

Kaynak :

Springer Nature

Dergi:

Scientific Reports

Yayıncı:

Springer Science and Business Media LLC

Yazar(lar) :

Gheyath K. Nasrallah , Soha R. Dargham , Farah Shurrab , Duaa W. Al-Sadeq , Hadeel Al-Jighefee , Hiam Chemaitelly , Zaina Al Kanaani , Abdullatif Al Khal , Einas Al Kuwari , Peter Coyle , Andrew Jeremijenko , Anvar Hassan Kaleeckal , Ali Nizar Latif , Riyazuddin Mohammad Shaik , Hanan F. Abdul Rahim , Hadi M. Yassine , Mohamed G. Al Kuwari , Hamda Qotba , Hamad Eid Al Romaihi , Patrick Tang , Roberto Bertollini , Mohamed H. Al-Thani , Asmaa A. Althani , Laith J. Abu-RaddadGheyath K. Nasrallah , Soha R. Dargham , Farah Shurrab , Duaa W. Al-Sadeq , Hadeel Al-Jighefee , Hiam Chemaitelly , Zaina Al Kanaani , Abdullatif Al Khal , Einas Al Kuwari , Peter Coyle , Andrew Jeremijenko , Anvar Hassan Kaleeckal , Ali Nizar Latif , Riyazuddin Mohammad Shaik , Hanan F. Abdul Rahim , Hadi M. Yassine , Mohamed G. Al Kuwari , Hamda Qotba , Hamad Eid Al Romaihi , Patrick Tang , Roberto Bertollini , Mohamed H. Al-Thani , Asmaa A. Althani , Laith J. Abu-Raddad

Yıl :

2021

Hadeel Al-Jighefee Hadeel Al-Jighefee

Yayıncı Web Sitesi

Tam Metin

Özet Ek Bilgiler

Kaydet

AbstractPerformance of three automated commercial serological IgG-based assays was investigated for assessing SARS-CoV-2 “ever” (past or current) infection in a population-based sample in a high exposure setting. PCR and serological testing was performed on 394 individuals. SARS-CoV-2-IgG seroprevalence was 42.9% (95% CI 38.1–47.8%), 40.6% (95% CI 35.9–45.5%), and 42.4% (95% CI 37.6–47.3%) using the CL-900i, VidasIII, and Elecsys assays, respectively. Between the three assays, overall, positive, and negative percent agreements ranged between 93.2–95.7%, 89.3–92.8%, and 93.8–97.8%, respectively; Cohen’s kappa statistic ranged from 0.86 to 0.91; and 35 specimens (8.9%) showed discordant results. Among all individuals, 12.5% (95% CI 9.6–16.1%) had current infection, as assessed by PCR. Of these, only 34.7% (95% CI 22.9–48.7%) were seropositive by at least one assay. A total of 216 individuals (54.8%; 95% CI 49.9–59.7%) had evidence of ever infection using antibody testing and/or PCR during or prior to this study. Of these, only 78.2%, 74.1%, and 77.3% were seropositive in the CL-900i, VidasIII, and Elecsys assays, respectively. All three assays had comparable performance and excellent agreement, but missed at least 20% of individuals with past or current infection. Commercial antibody assays can substantially underestimate ever infection, more so when infection rates are high.

DOI : 10.1038/s41598-021-91235-x

ISSN: 2045-2322
Sayı: 1
Cilt: 11

Performance evaluation of five ELISA kits for detecting anti-SARS-COV-2 IgG antibodies

Dergi Makalesi

Kaynak :

Science Direct

Dergi:

International Journal of Infectious Diseases

Yayıncı:

Elsevier

Yazar(lar) :

Hadi M. Yassine , Hadeel Al-Jighefee , Duaa W. Al-Sadeq , Soha R. Dargham , Salma N. Younes , Farah Shurrab , Reham M. Marei , Ali Ait Hssain , Sara Taleb , Hashim A. Alhussain , Maryiam A. Al-Nesf , Abdullatif Al-Khal , Hamda Qotba , Asmaa A. Althani , Patrick Tang , Laith J. Abu-Raddad , Gheyath K. Nasrallah

Yıl :

2020

Hadeel Al-Jighefee

Yayıncı Web Sitesi

Tam Metin

Ek Bilgiler

Kaydet

DOI : 10.1016/j.ijid.2020.10.042

ISSN: 1201-9712

Sınıflandırma :

Biyotıp %100

Parazitoloji ve Enfeksiyon Hastalıkları %54