Piri Keşif Aracı

Özet Ek Bilgiler

Kaydet

Animal tissues frozen without cryoprotection are thought to be inappropriate for use as a donor for somatic cell nuclear transfer (SCNT) studies. Cells in tissues that have been frozen without a cryoprotectant are commonly thought to be dead or to have lost genomic integrity. However, in this study we show that the frozen auricular cartilage tissues of anatolian buffalo contain a considerable number of viable healthy cells. The cells in auricular cartilage tissues are resistant to cryo-injury at −80 °C. Primary cell cultures were established from defrosted ear tissues which were frozen without cryoprotectant. The growth and functional characteristics of primary cell cultures are characterized according to cell growth curve, cell cycle analysis, karyotype and GAG synthesis. The results indicate that frozen cartilage tissues could be valuable materials for the conservation of species and SCNT technology.

Kaynak 1:

http://dx.doi.org/10.1016/j.cryobiol.2013.11.008 arayıcılığı görüntüle.
Kaynak 2:

https://aperta.ulakbim.gov.tr/record/90526 arayıcılığı görüntüle.

DOI : 10.1016/j.cryobiol.2013.11.008

Anahtar Kelimeler : Biobanking, Conservation, Cryoinjury, Cartilage, Gene preservation

ISSN: 0011-2240
Sayı: 1
Cilt: 68
Sayfa: 65-70

Sınıflandırma :

Yaşam Bilimleri %93

Kök hücreler %42

2

Development and characterization of anti-HBe monoclonal antibody

Dergi Makalesi

Kaynak 1:

Science Direct

Kaynak 2:

Aperta - TÜBİTAK Açık Arşivi

Dergi:

Current Opinion in Biotechnology

Yayıncı:

Elsevier

Yazar(lar) :

Ibrahim Sogut , Ibrahim Hatipoglu , Gungor Kanbak , Aynur Basalp

Yıl :

2011

Ibrahim Hatipoglu

Ek Bilgiler

Kaydet

Kaynak 1:

http://dx.doi.org/10.1016/j.copbio.2011.05.108 arayıcılığı görüntüle.
Kaynak 2:

https://aperta.ulakbim.gov.tr/record/92432 arayıcılığı görüntüle.

DOI : 10.1016/j.copbio.2011.05.108

ISSN: 0958-1669
Cilt: 22
Sayfa: S43

Sınıflandırma :

Biyotıp %62

Antikorlar %97

3

Measuring the Engagement Level of Political Parties with Public on Facebook: The Case of Turkey

Konferans Bildirileri

Kaynak :

IEEE Xplore

Yazar(lar) :

Mehmet Zahid Sobaci , Ibrahim Hatipoglu

Konu :

Communication, Networking and Broadcast Technologies, Components, Circuits, Devices and Systems, Computing and Processing

Yıl :

2017

Ibrahim Hatipoglu

Özet

Kaydet

Political parties have not been indifferent to the opportunities offered by the internet-based technologies. Today, political parties use the social media tools to communicate, organize and mobilize. However, few studies focus on the use of social media by the political parties and these studies analyze the use of social media in election campaigns. Therefore, in the relevant literature, there is a need for empirical studies to analyze the use of social media for political communication by political parties during nonelection periods. In this context, this study, using a metrics set, aims to analyze the engagement level and engagement type of the political parties with public in Turkey on Facebook as a social media tool in a non-election period. The findings of our analysis show that the engagement level of the political parties with public on Facebook in Turkey is low. Moreover, political parties in Turkey fail to communicate with the public in a bidirectional way on Facebook.

DOI : 10.1109/cedem.2017.15

Anahtar Kelimeler : Facebook, Tools, Measurement, Voting, Fans, Web 2.0, Internet, politics, social networking (online), social sciences computing, public engagement level, political parties, Facebook, Turkey, Internet, social media tools, election campaigns, Social media, political parties, public engagement, Facebook, Turkey

4

IRF4-dependent dendritic cells regulate CD8 + T-cell differentiation and memory responses in influenza infection

Dergi Makalesi

Kaynak :

Springer Nature

Dergi:

Mucosal Immunology

Yayıncı:

Springer Nature

Yazar(lar) :

Erola Ainsua-Enrich , Ibrahim Hatipoglu , Sapana Kadel , Sean Turner , Jinny Paul , Simar Singh , Harini Bagavant , Susan Kovats

Konu :

Biomedicine, general, Immunology, Allergology, Antibodies, Gastroenterology

Yıl :

2019

Ibrahim Hatipoglu

Özet Ek Bilgiler

Kaydet

Acute respiratory disease caused by influenza viruses is imperfectly mitigated by annual vaccination to select strains. Development of vaccines that elicit lung-resident memory CD8+ T cells (TRM) would offer more universal protection to seasonal and emerging pandemic viruses. Understanding how lung-resident dendritic cells (DCs) regulate TRM differentiation would be an important step in this process. Here, we used CD11c-cre-Irf4f/f (KO) mice, which lack lung-resident IRF4-dependent CD11b+CD24hi DCs and show IRF4 deficiency in other lung cDC subsets, to determine if IRF4-expressing DCs regulate CD8+ memory precursor cells and TRM during influenza A virus (IAV) infection. KO mice showed defective CD8+ T-cell memory, stemming from a deficit of T regulatory cells and memory precursor cells with decreased Foxo1 expression. Transfer of wild-type CD11b+CD24hi DCs into KO mice restored CD8+ memory precursor cell numbers to wild-type levels. KO mice recovered from a primary infection harbored reduced numbers of CD8+ TRM and showed deficient expansion of IFNγ+CD8+ T cells and increased lung pathology upon challenge with heterosubtypic IAV. Thus, vaccination strategies that harness the function of IRF4-dependent DCs could promote the differentiation of CD8+ TRM during IAV infection.

DOI : 10.1038/s41385-019-0173-1

ISSN: 1935-3456
Cilt: 12
Sayı: 4
Sayfa: 1025-1037

Sınıflandırma :

Biyotıp %100

Viroloji %60

Hematoloji %34

5

A Major Population of Functional KLRG1- ILC2s in Female Lungs Contributes to a Sex Bias in ILC2 Numbers.

Dergi Makalesi

Kaynak :

PubMed

Dergi:

Immunohorizons

Yazar(lar) :

Sapana Kadel , Erola Ainsua-Enrich , Ibrahim Hatipoglu , Sean Turner , Simar Singh , Sohaib Khan , Susan Kovats

Yıl :

2018

Ibrahim Hatipoglu

Piri Tam Metin Bulucu Özet Ek Bilgiler

Kaydet

Humans show significant sex differences in the incidence and severity of respiratory diseases, including asthma and virus infection. Sex hormones contribute to the female sex bias in type 2 inflammation associated with respiratory diseases, consistent with recent reports that female lungs harbor greater numbers of GATA-3-dependent group 2 innate lymphoid cells (ILC2s). In this study, we determined whether sex hormone levels govern sex differences in the numbers, phenotype, and function of ILC2s in the murine lung and bone marrow (BM). Our data show that lungs of female mice harbor significantly greater ILC2 numbers in homeostasis, in part due to a major subset of ILC2s lacking killer-cell lectin like receptor G1 (KLRG1), a population largely absent in male lungs. The KLRG1- ILC2s were capable of type 2 cytokine production and increased with age after sexual maturity, suggesting that a unique functional subset exists in females. Experiments with gonadectomized mice or mice bearing either global or lymphocyte restricted estrogen receptor α (Esr1) deficiency showed that androgens rather than estrogens regulated numbers of the KLRG1- ILC2 subset and ILC2 functional capacity in the lung and BM, as well as levels of GATA-3 expression in BM ILC2s. Furthermore, the frequency of BM PLZF+ ILC precursors was higher in males and increased by excess androgens, suggesting that androgens act to inhibit the transition of ILC precursors to ILC2s. Taken together, these data show that a functional subset of KLRG1- ILC2s in females contributes to the sex bias in lung ILC2s that is observed after reproductive age.

DOI : 10.4049/immunohorizons.1800008

Sınıflandırma :

Biyotıp %100

İnsan Genetiği %99

6

Hepatitis B virus e antigen (HBeAg) may have a negative effect on dendritic cell generation

Dergi Makalesi

Kaynak 1:

Science Direct

Kaynak 2:

Aperta - TÜBİTAK Açık Arşivi

Dergi:

Immunobiology

Yayıncı:

Elsevier

Yazar(lar) :

Ibrahim Hatipoglu , Duygu Ercan , Ceyda Acilan , Aynur Basalp , Deniz Durali , Ahmet Tarik Baykal

Yıl :

2014

Ibrahim Hatipoglu

Özet Ek Bilgiler

Kaydet

Hepatitis B virus (HBV) continues to be a serious worldwide health problem despite the use of protective HBV vaccines and therapeutic regimens against chronic HBV infection. Chronic HBV patients cannot induce sufficient immune responses against the virus. HBV and its antigens are believed to suppress immune responses during chronic infection. Hence, studying the role of HBV in immune suppression is very important for the development of alternative therapeutic strategies for HBV infections. In the present study, we investigated the effect of Hepatitis B virus e antigen (HBeAg) on the generation of bone marrow derived dendritic cells (BMDCs) and the stimulation of plasmacytoid DCs (pDCs). In the presence of HBeAg, the ratio of BMDCs was decreased, but the ratio of CD11b+Ly6G+ immature myeloid cells was increased. The expression of 47 proteins was also changed during HBeAg treatment; however, CpG-induced MHC-II expression on pDCs was not affected. Our results indicate that HBeAg may have a negative effect on the generation of DCs from bone morrow precursors.

Kaynak 1:

http://dx.doi.org/10.1016/j.imbio.2014.07.020 arayıcılığı görüntüle.
Kaynak 2:

https://aperta.ulakbim.gov.tr/record/90488 arayıcılığı görüntüle.

DOI : 10.1016/j.imbio.2014.07.020

Anahtar Kelimeler : HBV Hepatitis B virus, Hepatitis B virus, HBeAg Hepatitis B virus e antigen, Hepatitis B virus e antigen, IFN interferon, interferon, DC dendritic cell, dendritic cell, LCu2013MS/MS high-performance liquid chromatography coupled with electrospray tandem mass spectrometry, high-performance liquid chromatography coupled with electrospray tandem mass spectrometry, MDSC myeloid-derived suppressor cells, myeloid-derived suppressor cells, MHC-II major histocompatibility complex class II, major histocompatibility complex class II, pDC plasmacytoid dendritic cell, plasmacytoid dendritic cell, PDCA-1 plasmacytoid dendritic cell antigen-1, plasmacytoid dendritic cell antigen-1, OVA ovalbumin, ovalbumin, Dendritic cell, HBeAg, Hepatitis B virus, LCu2013MS/MS, Proteomics

ISSN: 0171-2985
Sayı: 12
Cilt: 219
Sayfa: 944-949

Sınıflandırma :

Biyotıp %100

Viroloji %99

7

Biological assays on the effects of Acra3 peptide from Turkish scorpion Androctonus crassicauda venom on a mouse brain tumor cell line (BC3H1) and production of specific monoclonal antibodies

Dergi Makalesi

Kaynak 1:

Science Direct

Kaynak 2:

Aperta - TÜBİTAK Açık Arşivi

Dergi:

Toxicon

Yayıncı:

Elsevier

Yazar(lar) :

Figen Caliskan , Emel Ergene , Ibrahim Sogut , Ibrahim Hatipoglu , Aynur Basalp , Hulya Sivas , Gungor Kanbak

Yıl :

2013

Ibrahim Hatipoglu

Özet Ek Bilgiler

Kaydet

Constitutes of the venom scorpion are a rich source of low molecular mass peptides which are toxic to various organisms, including man. Androctonus crassicauda is one of the scorpions from the Southeastern Anatolia of Turkey with public health importance. This work is focused on the investigation of biological effects of Acra3 peptide from Androctonus crassicauda. For this purpose, Acra3 isolated from crude venoms was tested for its cytotoxicity on BC3H1 mouse brain tumor cells using tetrazolium salt cleavage and lactate dehydrogenase activity assays. To determine whether the cytotoxic effects of Acra3 was related to the induction of apoptosis, the morphology of the cells and the nuclear fragmentation was examined by using Acridin Orange staining and DNA fragmentation assay, respectively. Caspase 3 and caspase 9 activities were measured spectrophotometrically and flow cytometric assay was performed using Annexin-V FITC and Propidium Iodide staining. Furthermore toxic peptide Acra3 was used as an antigen for immunological studies. Results showed that Acra3 exerted very strong cytotoxic effect on BC3H1 cells with an IC50 value of 5 μg/ml. Exposure of the cells to 0.1 and 0.5 μg/ml was resulted in very strong appearance of the apoptotic morphology in a dose dependent manner. On the other side, not any DNA fragmentation was observed after treatment of the cells. Caspase 3 and 9 activities were slightly decreased with Acra3. Results from flow cytometry and lactate dehydrogenase activity assays indicate that Acra3 exerts its effects by inducing a stronger necrosis than apoptosis in BC3H1 cells. To evaluate its immunogenicity, monoclonal antibody (MAb) specific for Acra3 antigen (5B9) was developed by hybridoma technology using spleen and lymph nodes of mice and immunoglobulin type of antibody was found to be IgM. We suggest that Acra3 may exert its effects by inducing both necrotic and apoptotic pathway in some way on mouse brain tumor cells. These findings will be useful for understanding the mechanism of cell death caused by venom in vitro. Anti-Acra3 monoclonal antibody can be further used as a bioactive tools for exploring the structure/function relationship and the pharmacological mechanism of scorpion peptide neurotoxins.

Kaynak 1:

http://dx.doi.org/10.1016/j.toxicon.2013.09.009 arayıcılığı görüntüle.
Kaynak 2:

https://aperta.ulakbim.gov.tr/record/89302 arayıcılığı görüntüle.

DOI : 10.1016/j.toxicon.2013.09.009

Anahtar Kelimeler : Scorpion toxin, Androctonus crassicauda, Cytotoxicity, Apoptosis, Immunization, Monoclonal antibodies

ISSN: 0041-0101
Cilt: 76
Sayfa: 350-361

Sınıflandırma :

Biyotıp %99

Antikorlar %100

8

The role of cell cycle progression for the apoptosis of cancer cells induced by palladium(II)-saccharinate complexes of terpyridine

Dergi Makalesi

Kaynak 1:

Science Direct

Kaynak 2:

Aperta - TÜBİTAK Açık Arşivi

Dergi:

Bioorganic & Medicinal Chemistry

Yayıncı:

Elsevier

Yazar(lar) :

Omer Kacar , Buse Cevatemre , Ibrahim Hatipoglu , Nazli Arda , Engin Ulukaya , Veysel T. Yilmaz , Ceyda Acilan

Yıl :

2017

Ibrahim Hatipoglu

Özet Ek Bilgiler

Kaydet

Palladium complexes are potent and less toxic molecules in comparison to other metal based agents. Here, we characterized two palladium(II) saccharinate complexes with terpyridine for their cell cycle specificity. Materials and methods Cells were arrested at G1, G1/S boundary or mitosis using mimosine, double-Thymidine block, aphidicolin, nocodazole or colcemid, and evaluated based on morphology and flow cytometry. Synchronized cells were treated with the Pd(II) complexes, and viability was measured via MTT assay. Results While treatment of arrested cells with the Pd(II) complexes resulted in no significant change in cell death in HCT-116 and MDA-MB-231 cells, HeLa cells were more sensitive in S/G1. The main form of cell death was found to be apoptosis. Conclusions Pd(II) complexes appear to be cell-cycle non-specific, while cell line dependent differences may be observed. Cells die through apoptosis regardless of the cell cycle stage, which makes these complexes more promising as anti-cancer agents.

Kaynak 1:

http://dx.doi.org/10.1016/j.bmc.2017.01.033 arayıcılığı görüntüle.
Kaynak 2:

https://aperta.ulakbim.gov.tr/record/98184 arayıcılığı görüntüle.

DOI : 10.1016/j.bmc.2017.01.033

Anahtar Kelimeler : Cancer, Metal based anticancer agents, Palladium(II) complex, Cell cycle specific, Apoptosis, Cytotoxicity, Cell cycle arrest

ISSN: 0968-0896
Sayı: 6
Cilt: 25
Sayfa: 1770-1777

9

Mammalian cell expression of Hepatitis B virus S antigen and immunization studies

Dergi Makalesi

Kaynak :

Science Direct

Dergi:

New Biotechnology

Yayıncı:

Elsevier

Yazar(lar) :

Aylin Ozdemir Bahadir , Duygu Hinc , Ibrahim Hatipoglu , B. Koray Balcioglu , Ibrahim Sogut , Aynur Basalp , Berrin Erdag

Yıl :

2012

Ibrahim Hatipoglu

Ek Bilgiler

Kaydet

DOI : 10.1016/j.nbt.2012.08.425

ISSN: 1871-6784
Cilt: 29
Sayfa: S153

Sınıflandırma :

Biyotıp %100

İnsan Genetiği %40

Viroloji %37

10

Generation of new protective HBV vaccine using the phage display method

Dergi Makalesi

Kaynak 1:

Science Direct

Kaynak 2:

Aperta - TÜBİTAK Açık Arşivi

Dergi:

Current Opinion in Biotechnology

Yayıncı:

Elsevier

Yazar(lar) :

Ibrahim Hatipoglu , Ibrahim Sogut , Koray Bertan Balcioglu , Aylin Ozdemir Bahadir , Serkan Uzyol , Hulya Sivas , Berrin Erdag , Aynur Basalp

Yıl :

2011

Ibrahim Hatipoglu

Ek Bilgiler

Kaydet

Kaynak 1:

http://dx.doi.org/10.1016/j.copbio.2011.05.107 arayıcılığı görüntüle.
Kaynak 2:

https://aperta.ulakbim.gov.tr/record/92362 arayıcılığı görüntüle.

DOI : 10.1016/j.copbio.2011.05.107

ISSN: 0958-1669
Cilt: 22
Sayfa: S43

Sınıflandırma :

Biyotıp %47

Antikorlar %95

Yaşam Bilimleri %44

Tıbbi Mikrobiyoloji %64

11

Antibody based systems for the determination of mycotoxins in food and feed

Dergi Makalesi

Kaynak 1:

Science Direct

Kaynak 2:

Aperta - TÜBİTAK Açık Arşivi

Dergi:

Current Opinion in Biotechnology

Yayıncı:

Elsevier

Yazar(lar) :

Ozlem Ertekin , Fatma Betul Guloglu , Seyda Pirincci , Sevecen Tuglu , Esin Akcael , Duygu Ercan , Ibrahim Hatipoglu , Meltem Goksel , Vefa Ahsen , Selma Ozturk

Yıl :

2013

Ibrahim Hatipoglu

Ek Bilgiler

Kaydet

Kaynak 1:

http://dx.doi.org/10.1016/j.copbio.2013.05.028 arayıcılığı görüntüle.
Kaynak 2:

https://aperta.ulakbim.gov.tr/record/91744 arayıcılığı görüntüle.

DOI : 10.1016/j.copbio.2013.05.028

ISSN: 0958-1669
Cilt: 24
Sayfa: S23

Sınıflandırma :

Kimya %85

Analitik Kimya %50

12

Phage Displayed HBV Core Antigen with Immunogenic Activity

Dergi Makalesi

Kaynak 1:

Springer Link

Kaynak 2:

Aperta - TÜBİTAK Açık Arşivi

Dergi:

Applied Biochemistry and Biotechnology

Yazar(lar) :

Aylin Ozdemir Bahadir , Bertan Koray Balcioglu , Kamil Serkan Uzyol , Ibrahim Hatipoglu , Ibrahim Sogut , Aynur Basalp , Berrin Erdag

Konu :

Molecular Biology, Applied Microbiology and Biotechnology, Biochemistry, General Medicine, Bioengineering, Biotechnology

Yıl :

2011

Ibrahim Hatipoglu

Özet Ek Bilgiler

Kaydet

Hepatitis B is a major public health problem worldwide, which may lead to chronic liver diseases such as cirrhosis and hepatocellular carcinoma. The hepatitis B core antigen (HBcAg) is one of the major viral proteins, which forms the inner core of hepatitis B virus (HBV) particles. In this study, filamentous bacteriophage M13 was genetically modified to display the polypeptides of HBcAg in order to develop an alternative carrier system. HBcAg gene was inserted into the minor coat protein (pIII) gene of M13, and HBcAg was expressed on the phage surface as a whole protein. Antigenicity and immunogenicity of HBcAg were tested by immunizing BALB/c mice three times with HBcAg-displaying recombinant phages. After successful immunization, one of the mice with high antibody titer to HBcAg was selected for fusion, and four monoclonal antibodies specific for HBcAg were developed. This result showed that HBcAg-displaying recombinant bacteriophages are immunogenic and can potentially be used for the development of monoclonal antibodies.

Kaynak 1:

http://dx.doi.org/10.1007/s12010-011-9365-1 arayıcılığı görüntüle.
Kaynak 2:

https://aperta.ulakbim.gov.tr/record/89418 arayıcılığı görüntüle.

DOI : 10.1007/s12010-011-9365-1

Anahtar Kelimeler : HBcAg, Phage display, M13 phage, Immune response, ELISA, Monoclonal antibody

ISSN: 0273-2289 1559-0291
Sayı: 7-8
Cilt: 165
Sayfa: 1437-1447

13

Monoclonal Antibodies Specific for Hepatitis B e Antigen and Hepatitis B Core Antigen

Araştırma Makalesi

Kaynak :

Aperta - TÜBİTAK Açık Arşivi

Dergi:

HYBRIDOMA

Yazar(lar) :

Aynur Basalp , Gungor Kanbak , Ibrahim Hatipoglu , Ibrahim Sogut

Yıl :

2011

Ibrahim Hatipoglu

Piri Tam Metin Bulucu Özet Ek Bilgiler

Kaydet

Despite effective vaccination programs in many countries, HBV infection is still a serious health problem throughout the world; more than 2 billion people have been infected with Hepatitis B virus (HBV). The serologic markers are crucial indicators in clinical diagnosis of HBV infection. The persistent presence of anti-HBc is associated with chronicity, and anti-HBe is an indicator for active viral replication. In the present study, two different hybridoma clones, 12E5 and 16F8, secreting anti-HBeAg and anti-HBc antibody were developed using hybridoma technology. BALB/c mice were immunized with HBe antigen (HBeAg), and monoclonal antibodies were generated from the spleen and lymph nodes of mice. Immunoglobulin types of antibodies were found to be IgG2a and IgG1, respectively. Monoclonal antibodies (MAbs) were produced in large scale, purified with affinity chromatography, and epitope analysis was performed. The results have shown that 12E5 and 16F8 monoclonal antibodies can be used for detection of HBcAg and HBeAg, indicating that they have the potential for use in clinical diagnosis.

DOI : 10.1089/hyb.2011.0052

Sayi :30
issue :5
Sayfa :475-479

Sınıflandırma :

Biyotıp %100

Antikorlar %85

14

Effects of fibronectin and type IV collagen on osteosarcoma cell apoptosis

Araştırma Makalesi

Kaynak :

Aperta - TÜBİTAK Açık Arşivi

Dergi:

INDIAN JOURNAL OF EXPERIMENTAL BIOLOGY

Yazar(lar) :

Gulsen Akalin Ciftci , Emel Ergene , Hulya Sivas , Ibrahim Hatipoglu , Zerrin Incesu

Yıl :

2013

Ibrahim Hatipoglu

Piri Tam Metin Bulucu Özet Ek Bilgiler

Kaydet

The aims of this study are the investigation of the effects of fibronectin and type IV collagen extracellular matrix proteins and the role of caspase-3 and -9 on cis-platin induced U2-OS apoptosis were studied. First the cytotoxic effects of cis-platin on cell system were investigated by colorimetric method and than morphological and ELISA analysis were used for determination of cell apoptosis when induced with dis-platin. In addition, after adhering the cells to fibronection or type IV collagen proteins, the apoptotic rate and the effects of caspase-3 and -9 were also investigated by ELISA in presence of specific inhibitors. U2-OS cells showed 20% cytotoxicity after treatment with 2.4 mu M of cis-platin for 48 h. Morphological and the numerical data showed that cis-platin was able to induced apoptosis on cells as a dose-dependent manner. Caspase-3 and -9 inhibitors inhibited cis-platin-induced apoptosis in U2-OS cells, respectively. The binding of cells to 10 mu g/mL of fibronectin but not type IV collagen enhanced the apoptosis about 2.5 fold that effects inhibited with caspase-3 inhibitor. The baspase-3 and -9 are involved in the apoptotic signals induced by cis-platin in U2-OS. The binding to fibronectin, but not type IV collagen enhanced the apoptotic response of U2-OS and fibronectin-dependent apoptosis was activated by caspase-3. These finding might be useful for patients to fight against osteosarcoma.

DOI : 10.81043/aperta.91769

Sayi :51
issue :10
Sayfa :789-796

Sınıflandırma :

Biyotıp %100

Apoptoz %91

15

Stimulation of dendritic cells with vaccine and vaccine-antibody complex and effect on immune response

Araştırma Makalesi

Kaynak :

Aperta - TÜBİTAK Açık Arşivi

Dergi:

TURKISH JOURNAL OF BIOLOGY

Yazar(lar) :

Aynur Basalp , Hulya Sivas , Soner Aksu , Duygu Ercan , Ibrahim Sogut , Ibrahim Hatipoglu

Yıl :

2013

Ibrahim Hatipoglu

Özet Ek Bilgiler

Kaydet

Dendritic cell (DC) vaccines are a promising and potent therapeutic tool for chronic diseases, autoimmune diseases, and cancer because of the unique ability of DCs to stimulate T cells. The challenge of DC vaccines is to find an effective form for antigen presentation. Although pure antigens, antigen complexes, plasmids, and mRNA have been used in different studies, no proper application to overcome this problem has been found yet. In this study, we investigated the eligibility of a commercial hepatitis B virus (HBV) vaccine or a vaccine-monoclonal antibody complex for antigen loading of DCs for a therapeutic purpose. DCs were derived from the bone marrow of transgenic hepatitis B (HBV-tg) mice using a granulocyte macrophage-colony stimulating factor and interleukin-4, and then loaded with a commercial HBV vaccine (containing hepatitis B virus surface antigens and aluminum hydroxide adjuvant) or a vaccine-antibody complex. HBV-tg mice were immunized with the vaccine and vaccine-antibody loaded DCs. Optimum HBV vaccine concentration and loading time were determined by 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium (WST-1) methods. Therapeutic effects of vaccine-antibody loaded DCs were determined by the evaluation of antibody response and hepatitis B surface expression levels in HBV-tg mice. Our results showed that commercial HBV vaccine loaded DCs induced humoral response in HBV-tg mice but had no effect on cellular immunity.

DOI : 10.3906/biy-1209-36

Sayi :37
issue :4
Sayfa :457-463

Sınıflandırma :

Biyotıp %100

Viroloji %96

16

CRYOPRESERVATION OF SPLEEN AND LYMPH NODES AS A SOURCE OF MONONUCLEAR CELLS TO BE USED FOR THE DEVELOPMENT OF MONOCLONAL ANTIBODY PRODUCING HYBRIDOMA CELLS

Araştırma Makalesi

Kaynak :

Aperta - TÜBİTAK Açık Arşivi

Dergi:

CRYOLETTERS

Yazar(lar) :

Aynur Basalp , Deniz Durali , Safak Isil Cevik , Muge Serhatli , Ibrahim Hatipoglu , Ibrahim Sogut

Yıl :

2011

Ibrahim Hatipoglu

Piri Tam Metin Bulucu Özet Ek Bilgiler

Kaydet

In the present study, spleen and lymph nodes of mice were cryopreserved as a whole tissue and after thawing, membrane integrity of mononuclear cells was determined by trypan blue exclusion and PI staining. T and B lymphocytes, macrophages and dendritic cells have been isolated from both cryopreserved tissue and analyzed by Flow cytometry. BALB/c mice were immunized with Hepatitis e antigen (HBeAg) and spleen and lymph nodes of mice were cryopreserved for 3 to 10 months. The cells obtained from both tissue were applied to hybridoma technology to understand if the cells keep their viability and functionality. The cells were isolated and fused with F0 mouse myeloma cells and several antibody producing hybrid cells were developed. Results have shown that cryopreserved spleen and lymph nodes of mice can be efficiently used in hybridoma technology for the successful generation of monoclonal antibody producing hybrid cells.

DOI : 10.81043/aperta.89559

Sayi :32
issue :3
Sayfa :266-274

Sınıflandırma :

Biyotıp %100

Antikorlar %88

17

Human blood myeloid and plasmacytoid dendritic cells cross activate each other and synergize in inducing NK cell cytotoxicity

Dergi Makalesi

Kaynak :

Taylor and Francis

Dergi:

OncoImmunology

Yayıncı:

Informa UK Limited

Yazar(lar) :

Jasper J. P. van Beek , Mark A. J. Gorris , Annette E. Sköld , Ibrahim Hatipoglu , Heleen H. Van Acker , Evelien L. Smits , I. Jolanda M. de Vries , Ghaith Bakdash

Yıl :

2016

Ibrahim Hatipoglu