Piri Keşif Aracı

Ek Bilgiler

Kaydet

Kaynak 1:

http://dx.doi.org/10.1016/j.copbio.2011.05.108 arayıcılığı görüntüle.
Kaynak 2:

https://aperta.ulakbim.gov.tr/record/92432 arayıcılığı görüntüle.

DOI : 10.1016/j.copbio.2011.05.108

ISSN: 0958-1669
Cilt: 22
Sayfa: S43

Sınıflandırma :

Biyotıp %62

Antikorlar %97

2

Prenatal alcohol–induced neuroapoptosis in rat brain cerebral cortex: protective effect of folic acid and betaine

Dergi Makalesi

Kaynak :

Springer Link

Dergi:

Child's Nervous System

Yazar(lar) :

Ibrahim Sogut , Onur Uysal , Aysegul Oglakci , Ferruh Yucel , Kazim Kartkaya , Gungor Kanbak

Konu :

Clinical Neurology, General Medicine, Pediatrics, Perinatology, and Child Health

Yıl :

2017

Ibrahim Sogut

Özet Ek Bilgiler

Kaydet

Alcohol consumption in pregnancy may cause fetal alcohol syndrome (FAS) in the infant. This study aims to investigate prenatal alcohol exposure related neuroapoptosis on the cerebral cortex tissues of newborn rats and possible neuroprotective effects of betaine, folic acid, and combined therapy. Pregnant rats were divided into five experimental groups: control, ethanol, ethanol + betaine, ethanol + folic acid, and ethanol + betaine + folic acid combined therapy groups. We measured cytochrome c release, caspase-3, calpain and cathepsin B and L. enzyme activities. In order to observe apoptotic cells in the early stages, TUNEL method was chosen together with histologic methods such as assessing the diameters of the apoptotic cells, their distribution in unit volume and volume proportion of cortical intact neuron nuclei. Calpain, caspase-3 activities, and cytochrome c levels were significantly increased in alcohol group while cathepsin B and L. activities were also found to be elevated albeit not statistically significant. These increases were significantly reversed by folic acid and betaine + folic acid treatments. While ethanol increased the number of apoptotic cells, this increase was prevented in ethanol + betaine and ethanol + betaine + folic acid groups. Morphometric examination showed that the mean diameter of apoptotic cells was increased with ethanol administration while this increase was reduced by betaine and betaine + folic acid treatments. We observed that ethanol is capable of triggering apoptotic cell death in the newborn rat brains. Furthermore, folic acid, betaine, and combined therapy of these supplements may reduce neuroapoptosis related to prenatal alcohol consumption, and might be effective on preventing fetal alcohol syndrome in infants.

DOI : 10.1007/s00381-016-3309-6

Anahtar Kelimeler : Betaine, Calpain, Caspase-3, Cytochrome c, Folic acid, Neuroapoptosis

ISSN: 0256-7040 1433-0350
Sayı: 3
Cilt: 33
Sayfa: 407-417

3

Biological assays on the effects of Acra3 peptide from Turkish scorpion Androctonus crassicauda venom on a mouse brain tumor cell line (BC3H1) and production of specific monoclonal antibodies

Dergi Makalesi

Kaynak 1:

Science Direct

Kaynak 2:

Aperta - TÜBİTAK Açık Arşivi

Dergi:

Toxicon

Yayıncı:

Elsevier

Yazar(lar) :

Figen Caliskan , Emel Ergene , Ibrahim Sogut , Ibrahim Hatipoglu , Aynur Basalp , Hulya Sivas , Gungor Kanbak

Yıl :

2013

Ibrahim Sogut

Özet Ek Bilgiler

Kaydet

Constitutes of the venom scorpion are a rich source of low molecular mass peptides which are toxic to various organisms, including man. Androctonus crassicauda is one of the scorpions from the Southeastern Anatolia of Turkey with public health importance. This work is focused on the investigation of biological effects of Acra3 peptide from Androctonus crassicauda. For this purpose, Acra3 isolated from crude venoms was tested for its cytotoxicity on BC3H1 mouse brain tumor cells using tetrazolium salt cleavage and lactate dehydrogenase activity assays. To determine whether the cytotoxic effects of Acra3 was related to the induction of apoptosis, the morphology of the cells and the nuclear fragmentation was examined by using Acridin Orange staining and DNA fragmentation assay, respectively. Caspase 3 and caspase 9 activities were measured spectrophotometrically and flow cytometric assay was performed using Annexin-V FITC and Propidium Iodide staining. Furthermore toxic peptide Acra3 was used as an antigen for immunological studies. Results showed that Acra3 exerted very strong cytotoxic effect on BC3H1 cells with an IC50 value of 5 μg/ml. Exposure of the cells to 0.1 and 0.5 μg/ml was resulted in very strong appearance of the apoptotic morphology in a dose dependent manner. On the other side, not any DNA fragmentation was observed after treatment of the cells. Caspase 3 and 9 activities were slightly decreased with Acra3. Results from flow cytometry and lactate dehydrogenase activity assays indicate that Acra3 exerts its effects by inducing a stronger necrosis than apoptosis in BC3H1 cells. To evaluate its immunogenicity, monoclonal antibody (MAb) specific for Acra3 antigen (5B9) was developed by hybridoma technology using spleen and lymph nodes of mice and immunoglobulin type of antibody was found to be IgM. We suggest that Acra3 may exert its effects by inducing both necrotic and apoptotic pathway in some way on mouse brain tumor cells. These findings will be useful for understanding the mechanism of cell death caused by venom in vitro. Anti-Acra3 monoclonal antibody can be further used as a bioactive tools for exploring the structure/function relationship and the pharmacological mechanism of scorpion peptide neurotoxins.

Kaynak 1:

http://dx.doi.org/10.1016/j.toxicon.2013.09.009 arayıcılığı görüntüle.
Kaynak 2:

https://aperta.ulakbim.gov.tr/record/89302 arayıcılığı görüntüle.

DOI : 10.1016/j.toxicon.2013.09.009

Anahtar Kelimeler : Scorpion toxin, Androctonus crassicauda, Cytotoxicity, Apoptosis, Immunization, Monoclonal antibodies

ISSN: 0041-0101
Cilt: 76
Sayfa: 350-361

Sınıflandırma :

Biyotıp %99

Antikorlar %100

4

Mammalian cell expression of Hepatitis B virus S antigen and immunization studies

Dergi Makalesi

Kaynak :

Science Direct

Dergi:

New Biotechnology

Yayıncı:

Elsevier

Yazar(lar) :

Aylin Ozdemir Bahadir , Duygu Hinc , Ibrahim Hatipoglu , B. Koray Balcioglu , Ibrahim Sogut , Aynur Basalp , Berrin Erdag

Yıl :

2012

Ibrahim Sogut

Ek Bilgiler

Kaydet

DOI : 10.1016/j.nbt.2012.08.425

ISSN: 1871-6784
Cilt: 29
Sayfa: S153

Sınıflandırma :

Biyotıp %100

İnsan Genetiği %40

Viroloji %37

5

Phage Displayed HBV Core Antigen with Immunogenic Activity

Dergi Makalesi

Kaynak 1:

Springer Link

Kaynak 2:

Aperta - TÜBİTAK Açık Arşivi

Dergi:

Applied Biochemistry and Biotechnology

Yazar(lar) :

Aylin Ozdemir Bahadir , Bertan Koray Balcioglu , Kamil Serkan Uzyol , Ibrahim Hatipoglu , Ibrahim Sogut , Aynur Basalp , Berrin Erdag

Konu :

Molecular Biology, Applied Microbiology and Biotechnology, Biochemistry, General Medicine, Bioengineering, Biotechnology

Yıl :

2011

Ibrahim Sogut

Özet Ek Bilgiler

Kaydet

Hepatitis B is a major public health problem worldwide, which may lead to chronic liver diseases such as cirrhosis and hepatocellular carcinoma. The hepatitis B core antigen (HBcAg) is one of the major viral proteins, which forms the inner core of hepatitis B virus (HBV) particles. In this study, filamentous bacteriophage M13 was genetically modified to display the polypeptides of HBcAg in order to develop an alternative carrier system. HBcAg gene was inserted into the minor coat protein (pIII) gene of M13, and HBcAg was expressed on the phage surface as a whole protein. Antigenicity and immunogenicity of HBcAg were tested by immunizing BALB/c mice three times with HBcAg-displaying recombinant phages. After successful immunization, one of the mice with high antibody titer to HBcAg was selected for fusion, and four monoclonal antibodies specific for HBcAg were developed. This result showed that HBcAg-displaying recombinant bacteriophages are immunogenic and can potentially be used for the development of monoclonal antibodies.

Kaynak 1:

http://dx.doi.org/10.1007/s12010-011-9365-1 arayıcılığı görüntüle.
Kaynak 2:

https://aperta.ulakbim.gov.tr/record/89418 arayıcılığı görüntüle.

DOI : 10.1007/s12010-011-9365-1

Anahtar Kelimeler : HBcAg, Phage display, M13 phage, Immune response, ELISA, Monoclonal antibody

ISSN: 0273-2289 1559-0291
Sayı: 7-8
Cilt: 165
Sayfa: 1437-1447

6

Generation of new protective HBV vaccine using the phage display method

Dergi Makalesi

Kaynak 1:

Science Direct

Kaynak 2:

Aperta - TÜBİTAK Açık Arşivi

Dergi:

Current Opinion in Biotechnology

Yayıncı:

Elsevier

Yazar(lar) :

Ibrahim Hatipoglu , Ibrahim Sogut , Koray Bertan Balcioglu , Aylin Ozdemir Bahadir , Serkan Uzyol , Hulya Sivas , Berrin Erdag , Aynur Basalp

Yıl :

2011

Ibrahim Sogut

Ek Bilgiler

Kaydet

Kaynak 1:

http://dx.doi.org/10.1016/j.copbio.2011.05.107 arayıcılığı görüntüle.
Kaynak 2:

https://aperta.ulakbim.gov.tr/record/92362 arayıcılığı görüntüle.

DOI : 10.1016/j.copbio.2011.05.107

ISSN: 0958-1669
Cilt: 22
Sayfa: S43

Sınıflandırma :

Biyotıp %47

Antikorlar %95

Yaşam Bilimleri %44

Tıbbi Mikrobiyoloji %64

7

Effect of fulvic acid on gastric mucosa damage caused by chronic water avoidance stress

Dergi Makalesi

Kaynak :

Taylor and Francis

Dergi:

Biotechnic & Histochemistry

Yayıncı:

Informa UK Limited

Yazar(lar) :

Sezen Gizem Gurel , Ibrahim Sogut , Canan Hurdag , Ali Gurel , Ahmet Tutar , Esra Cikler-Dulger

Yıl :

2021

Ibrahim Sogut

Özet Ek Bilgiler

Kaydet

We investigated the antioxidant and anti-ulcerogenic effects of fulvic acid (FA) on oxidative damage caused by water avoidance stress (WAS) in rat gastrointestinal mucosa. Three experimental groups were established: control (C), chronic stress (CS), and chronic stress + FA (CS + FA). After WAS, a single dose of FA was administered for 10 days to the CS + FA group. Samples of the pyloric region of the stomach were stained with hematoxylin and eosin (H & E) and periodic acid-Schiff (PAS). Immunohistochemical staining was performed for inducible nitric oxide synthase (iNOS) and endothelial nitric oxide synthase (eNOS). Total antioxidant status (TAS), total oxidant status (TOS), oxidative stress index (OSI), superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAT) levels were measured biochemically. By light microscopy, we observed loss of gastric epithelial cells and greater polymorphonuclear cell migration into the mucosa in the CS group compared to the C group. We found intact epithelial cell structure and a thick superficial mucus layer in the CS + FA group compared to the CS group. These findings in the CS + FA group were similar to those for group C. iNOS staining was stronger in the CS group compared to the C group. TOS and OSI levels in the CS + FA group were decreased compared to the CS group, but TAS, SOD, GPx and CAT levels were increased. We found that WAS caused damage to epithelium and connective tissue of the stomach mucosa and that this damage was prevented by FA. Therefore, administration of FA appears to prevent stress induced damage to rat stomach.

DOI : 10.1080/10520295.2021.1926541

Anahtar Kelimeler : Antioxidant, fulvic acid, gastric mucosa, inflammation, oxidative stress, rats, stomach, stress, water avoidance

ISSN: 1052-0295
Sayfa: 1-8

8

Monoclonal Antibodies Specific for Hepatitis B e Antigen and Hepatitis B Core Antigen

Araştırma Makalesi

Kaynak :

Aperta - TÜBİTAK Açık Arşivi

Dergi:

HYBRIDOMA

Yazar(lar) :

Aynur Basalp , Gungor Kanbak , Ibrahim Hatipoglu , Ibrahim Sogut

Yıl :

2011

Ibrahim Sogut

Piri Tam Metin Bulucu Özet Ek Bilgiler

Kaydet

Despite effective vaccination programs in many countries, HBV infection is still a serious health problem throughout the world; more than 2 billion people have been infected with Hepatitis B virus (HBV). The serologic markers are crucial indicators in clinical diagnosis of HBV infection. The persistent presence of anti-HBc is associated with chronicity, and anti-HBe is an indicator for active viral replication. In the present study, two different hybridoma clones, 12E5 and 16F8, secreting anti-HBeAg and anti-HBc antibody were developed using hybridoma technology. BALB/c mice were immunized with HBe antigen (HBeAg), and monoclonal antibodies were generated from the spleen and lymph nodes of mice. Immunoglobulin types of antibodies were found to be IgG2a and IgG1, respectively. Monoclonal antibodies (MAbs) were produced in large scale, purified with affinity chromatography, and epitope analysis was performed. The results have shown that 12E5 and 16F8 monoclonal antibodies can be used for detection of HBcAg and HBeAg, indicating that they have the potential for use in clinical diagnosis.

DOI : 10.1089/hyb.2011.0052

Sayi :30
issue :5
Sayfa :475-479

Sınıflandırma :

Biyotıp %100

Antikorlar %85

9

Effect of chronic alcohol consumption on myocardial apoptosis in the rat model of isoproterenol-induced myocardial injury and investigation on the cardioprotective role of calpain inhibitor 1

Dergi Makalesi

Kaynak :

Taylor and Francis

Dergi:

Drug and Chemical Toxicology

Yayıncı:

Informa UK Limited

Yazar(lar) :

Aysegul Oglakci-Ilhan , Kevser Kusat-Ol , Kubilay Uzuner , Onur Uysal , Ibrahim Sogut , Ferruh Yucel , Gungor Kanbak

Yıl :

2021

Ibrahim Sogut

Özet Ek Bilgiler

Kaydet

We investigated the presence of myocardial apoptosis on isoproterenol (ISO)-induced myocardial injury (MI) after long-term high dose alcohol consumption and examined the antiapoptotic role of calpain inhibitor 1. Male Wistar Albino rats (n = 108) were divided into six groups: Control, alcohol (ethanol was given during 30 days for chronic alcohol consumption), MI (150 mg/kg ISO injection at last two days of alcohol consumption), alcohol + MI, alcohol + MI + calpain inhibitor 1 (10 mg/kg inhibitor was injected at 15 min before ISO injections) and Dimethyl Sulfoxide (DMSO) groups. Biochemical, histological, and morphometric methods determined apoptosis levels in the heart tissue of rats. Cytochrome c, caspase 3, and calpain levels were significantly high in alcohol, MI, and alcohol + MI groups. In contrast, mitochondrial cardiolipin content was found to be low in alcohol, MI, and alcohol + MI groups. These parameters were close to the control group in the therapy group. Histological and morphometric data have supported biochemical results. As a result of our biochemical data, myocardial apoptosis was seen in the alcohol, MI, and especially alcohol after MI groups. Calpain inhibitor 1 reduced apoptotic cell death and prevented myocardial tissue injury in these groups. The efficiency of calpain inhibitor was very marked in MI after long-term high dose alcohol consumption.

DOI : 10.1080/01480545.2021.1985910

Anahtar Kelimeler : Alcohol, apoptosis, calpain inhibitor, myocardial injury, rat

ISSN: 0148-0545
Sayfa: 1-12

10

Stimulation of dendritic cells with vaccine and vaccine-antibody complex and effect on immune response

Araştırma Makalesi

Kaynak :

Aperta - TÜBİTAK Açık Arşivi

Dergi:

TURKISH JOURNAL OF BIOLOGY

Yazar(lar) :

Aynur Basalp , Hulya Sivas , Soner Aksu , Duygu Ercan , Ibrahim Sogut , Ibrahim Hatipoglu

Yıl :

2013

Ibrahim Sogut

Özet Ek Bilgiler

Kaydet

Dendritic cell (DC) vaccines are a promising and potent therapeutic tool for chronic diseases, autoimmune diseases, and cancer because of the unique ability of DCs to stimulate T cells. The challenge of DC vaccines is to find an effective form for antigen presentation. Although pure antigens, antigen complexes, plasmids, and mRNA have been used in different studies, no proper application to overcome this problem has been found yet. In this study, we investigated the eligibility of a commercial hepatitis B virus (HBV) vaccine or a vaccine-monoclonal antibody complex for antigen loading of DCs for a therapeutic purpose. DCs were derived from the bone marrow of transgenic hepatitis B (HBV-tg) mice using a granulocyte macrophage-colony stimulating factor and interleukin-4, and then loaded with a commercial HBV vaccine (containing hepatitis B virus surface antigens and aluminum hydroxide adjuvant) or a vaccine-antibody complex. HBV-tg mice were immunized with the vaccine and vaccine-antibody loaded DCs. Optimum HBV vaccine concentration and loading time were determined by 2-(4-iodophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium (WST-1) methods. Therapeutic effects of vaccine-antibody loaded DCs were determined by the evaluation of antibody response and hepatitis B surface expression levels in HBV-tg mice. Our results showed that commercial HBV vaccine loaded DCs induced humoral response in HBV-tg mice but had no effect on cellular immunity.

DOI : 10.3906/biy-1209-36

Sayi :37
issue :4
Sayfa :457-463

Sınıflandırma :

Biyotıp %100

Viroloji %96

11

CRYOPRESERVATION OF SPLEEN AND LYMPH NODES AS A SOURCE OF MONONUCLEAR CELLS TO BE USED FOR THE DEVELOPMENT OF MONOCLONAL ANTIBODY PRODUCING HYBRIDOMA CELLS

Araştırma Makalesi

Kaynak :

Aperta - TÜBİTAK Açık Arşivi

Dergi:

CRYOLETTERS

Yazar(lar) :

Aynur Basalp , Deniz Durali , Safak Isil Cevik , Muge Serhatli , Ibrahim Hatipoglu , Ibrahim Sogut

Yıl :

2011

Ibrahim Sogut

Piri Tam Metin Bulucu Özet Ek Bilgiler

Kaydet

In the present study, spleen and lymph nodes of mice were cryopreserved as a whole tissue and after thawing, membrane integrity of mononuclear cells was determined by trypan blue exclusion and PI staining. T and B lymphocytes, macrophages and dendritic cells have been isolated from both cryopreserved tissue and analyzed by Flow cytometry. BALB/c mice were immunized with Hepatitis e antigen (HBeAg) and spleen and lymph nodes of mice were cryopreserved for 3 to 10 months. The cells obtained from both tissue were applied to hybridoma technology to understand if the cells keep their viability and functionality. The cells were isolated and fused with F0 mouse myeloma cells and several antibody producing hybrid cells were developed. Results have shown that cryopreserved spleen and lymph nodes of mice can be efficiently used in hybridoma technology for the successful generation of monoclonal antibody producing hybrid cells.

DOI : 10.81043/aperta.89559

Sayi :32
issue :3
Sayfa :266-274

Sınıflandırma :

Biyotıp %100

Antikorlar %88

12

The antioxidant and antiapoptotic effect of boric acid on hepatoxicity in chronic alcohol-fed rats

Araştırma Makalesi

Kaynak :

Aperta - TÜBİTAK Açık Arşivi

Dergi:

CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY

Yazar(lar) :

Canan Hurdag , Melike Ersoz , Matem Tuncdemir , Sila Ozlem Paltun , Ibrahim Sogut

Yıl :

2018

Ibrahim Sogut

Piri Tam Metin Bulucu Özet Ek Bilgiler

Kaydet

The harmful use of alcohol is a worldwide problem involving all ages. This study aims to investigate chronic alcohol exposure related hepatotoxicity on the rat liver and possible hepatoprotective effects of boric acid. Rats were separated into 4 different groups: control, ethanol, ethanol+boric acid, and boric acid. We measured (i) malondialdehyde (MDA), total sialic acid (TSA), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) levels, which are known to be the markers of alcohol damage; and also (ii) caspase-3, tumor necrosis factor-alpha (TNF-alpha), and the terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) as the markers of apoptosis. In the ethanol group, MDA, TSA, and TNF-alpha levels increased whereas SOD and CAT levels decreased compared with the control group. Ethanol+boric acid group MDA, TSA, caspase-3, and TNF-alpha levels decreased whereas SOD and CAT levels increased compared with the ethanol group. Using histopathological evaluation of light microscope images, immunohistochemical caspase-3 and TNF-alpha activity in the ethanol+boric acid group were shown to be decreased compared with that in the ethanol group. Our results revealed that ethanol is capable of triggering oxidative stress and apoptosis in the rat liver. We propose that boric acid is an effective compound in protecting the rat liver against ethanol.

DOI : 10.1139/cjpp-2017-0487

Sayi :96
issue :4
Sayfa :404-411

Sınıflandırma :

Biyotıp %100

Biyotıp %93

13

Prenatal alcohol-induced neuroapoptosis in rat brain cerebral cortex: protective effect of folic acid and betaine

Araştırma Makalesi

Kaynak :

Aperta - TÜBİTAK Açık Arşivi

Dergi:

CHILDS NERVOUS SYSTEM

Yazar(lar) :

Gungor Kanbak , Kazim Kartkaya , Ferruh Yucel , Aysegul Oglakci , Onur Uysal , Ibrahim Sogut

Yıl :

2017

Ibrahim Sogut