Piri Keşif Aracı

Özet

Kaydet

More electrically powered aircraft reveals some significant advantages such as weight decrease, reduced maintenance requirements and increased reliability and passenger comfort. However, the power transformers as essential components in the future aircraft can be suffering from transient inrush currents which are several times of the rated value. Inrush current test is becoming an indispensable task in the validation/verification phase of More Electric Aircraft design process. A physical modeling approach to describe the inrush current characteristics of power transformers have been proposed in this paper. Based on the physical models of power transformers, comprehensive studies of inrush current such as sympathetic phenomenon can be conveniently performed by using simulation frameworks like Dymola.

DOI : 10.1109/ifeec.2013.6687597

Anahtar Kelimeler : Surges, Aircraft, Atmospheric modeling, Load modeling, Power transformers, Equations, Mathematical model, aircraft power systems, maintenance engineering, power transformers, reliability, Dymola, simulation frameworks, sympathetic phenomenon, inrush current characteristics, physical modeling approach, more-electric aircraft design process, validation-verification phase, inrush current test, transient inrush current, passenger comfort, reliability, maintenance requirement, power transformers

2

Time-related Histopathologic Changes in Fresh Frozen Carotid Xenografts in a Pig-to-Goat Implantation Model

Dergi Makalesi

Kaynak :

Wiley Online Library

Dergi:

Artificial Organs

Yazar(lar) :

Ji M. Chang , Won G. Kim

Konu :

Medicine (miscellaneous), Bioengineering, Biomaterials, Biomedical Engineering, General Medicine

Yıl :

2009

Ji M. Chang

Özet Ek Bilgiler

Kaydet

We performed an animal experiment with an emphasis on time‐related histopathologic changes to evaluate the clinical feasibility of immunologically nontreated xenogenic vascular grafts. Bilateral porcine carotid arteries were harvested, and then, after short‐term freezing at −70°C, interposed into goats' carotid arteries. An antiplatelet agent was administered orally for 3 months postoperatively. The goats were randomly assigned to five periods of observation (1 week, and 1, 3, 6, and 12 months after implantation); two animals were observed at each of these times. Doppler ultrasonography was performed periodically during the observation period. At predetermined times, grafts were explanted and examined using hematoxylin and eosin, and Masson's trichrome stains. Immunohistochemical evaluations were conducted with T‐lymphocyte indicator and von Willebrand factor. Two goats died prematurely, one from respiratory problems related to anesthesia and the other from pneumonia. A total of 16 grafts from the remaining eight animals were evaluated. Grafts were all patent except one at 3 months after transplantation. Histologically, xenogenic arterial grafts showed early endothelial cell loss at 1 week. This was followed by a progressive spread of recipient endothelial cells from the anastomotic site, and re‐endothelialization was complete at 1 month. The degree of neointimal and medial thickening increased until 3 months, and then decreased. At 12 months, no additional growth of the intimal or medial layers was observed. Adventitial inflammation became severe at 3 months, but was reduced at 6–12 months. The proportions of CD3‐positive T‐lymphocytes among inflammatory cell infiltrations were very low. Fresh frozen xenogenic arterial grafts showed acceptable patency throughout the 12‐month period and showed no evidence of being unduly influenced by rejection reactions.

DOI : 10.1111/j.1525-1594.2009.00819.x

ISSN: 0160-564X
Sayı: 10
Cilt: 33
Sayfa: 827-834

3

Hepatic Arterial Infusion Combined with Systemic Chemotherapy for Patients with Extensive Liver Metastases from Gastric Cancer

Dergi Makalesi

Kaynak :

Directory of Open Access Journals (DOAJ)

Dergi:

Cancer Management and Research

Yazar(lar) :

Qiang W , Shi H , Wu J , Ji M , Wu C

Yıl :

2020

Ji M

Özet Ek Bilgiler

Kaydet

Weiguang Qiang,1 Hongbing Shi,1 Jun Wu,1 Mei Ji,1 Changping Wu1,2 1Department of Oncology, The Third Affiliated Hospital of Soochow University, Changzhou, People’s Republic of China; 2Department of Tumor Biological Treatment, The Third Affiliated Hospital of Soochow University, Changzhou, People’s Republic of ChinaCorrespondence: Changping Wu; Weiguang QiangDepartment of Oncology, The Third Affiliated Hospital of Soochow University, Changzhou, Jiangsu Province, People’s Republic of ChinaEmail wcpjjt@163.com; qwgwutongyu@163.comPurpose: Liver metastases in patients with gastric cancer often indicate poor prognosis. Once liver metastases are extensive, it is difficult to achieve disease control by using systemic chemotherapy alone. The purpose of this study was to evaluate the effect and safety of hepatic arterial infusion (HAI) combined with systemic chemotherapy on extensive liver metastases from gastric cancer.Patients and Methods: Between 2012 and 2019, 21 patients with extensive liver metastases from gastric cancer (LMGC) were enrolled in our study. Liver metastases were identified as unresectable and a major factor affecting prognosis mainly based on size and number of intrahepatic lesions. All patients received systemic chemotherapy with S-1 and HAI oxaliplatin plus floxuridine (FUDR).Results: Liver metastases in 16 patients (76.2%) were evaluated as H3. The overall response rate was 76.2% (9.5% complete response). Intrahepatic and extrahepatic median progression-free survival times were 9.5 and 5.2 months, respectively. Median survival time (MST) was 12.3 months. All patients did not have the toxicity of grade 4. Grade 3 toxic effects included bone marrow suppression (14.3%) and diarrhea (9.5%). The other treatment-related toxicities were mild and reversible.Conclusion: HAI combined with systemic chemotherapy for extensive LMGC seems to be safe and effective, which achieves a high-local response and may contribute to long survival time for patients.Keywords: gastric cancer, liver metastases, hepatic arterial infusion, systemic chemotherapy

Anahtar Kelimeler : gastric cancer, liver metastases, hepatic arterial infusion, systemic chemotherapy

ISSN: 1179-1322
Cilt: 12
Sayfa: 2911 - 2916

Sınıflandırma :

Tıp ve Halk Sağlığı %98

Cerrahi Onkoloji %97

4

Mutational analysis on gastric, duodenal, bone, and mediastinal lymph node metastases and blood from a case of primary lung adenocarcinoma

Dergi Makalesi

Kaynak :

Directory of Open Access Journals (DOAJ)

Dergi:

OncoTargets and Therapy

Yazar(lar) :

Yang X , Chen R , Wu C , Zhao W , Ji M

Yıl :

2018

Ji M

Özet Ek Bilgiler

Kaydet

Xin Yang, Rui Chen, Chen Wu, Weiqing Zhao, Mei Ji Department of Oncology, The Third Affiliated Hospital of Soochow University, Changzhou 213003, China Abstract: Rapid metastasis contributes significantly to the high mortality rates in lung adenocarcinoma. The present study reports a rare case of primary lung adenocarcinoma with concomitant gastric, duodenal, bone, and mediastinal lymph node metastases. A large gene panel based on the next-generation sequencing was used to detect gene mutations in different metastatic sites and blood. The results showed that the gene mutation spectrums among different metastatic foci were roughly similar. The mutation abundance was highest in mediastinal lymph nodes. Unique mutation sites were detected only in mediastinal lymph nodes, bone, and gastric metastatic foci. Despite partial heterogeneity, there are currently no applicable targeted drugs to adopt. In addition, tumor mutation burden (TMB) showed that blood, gastric, bone, and mediastinal lymph node metastases were all TMB-High, while the duodenal metastasis was TMB-Low. This study is the first to report a rare case of newly diagnosed lung adenocarcinoma with concomitant gastrointestinal metastases and to perform mutation analyses on all metastatic foci. Large sample sizes with similar cases are required to gain deeper insights. Keywords: lung adenocarcinoma, gastric metastasis, duodenal metastasis, TMB

Anahtar Kelimeler : lung adenocarcinoma, gastric metastasis, duodenal metastasis

ISSN: 1178-6930
Cilt: 11
Sayfa: 4029 - 4034

Sınıflandırma :

Cerrahi Onkoloji %98

5

Therapeutic advantage from combining 5-fluorouracil with the hypoxia-selective cytotoxin NLCQ-1 in vivo; comparison with tirapazamine

Dergi Makalesi

Kaynak :

Springer Link

Dergi:

Cancer Chemotherapy and Pharmacology

Yazar(lar) :

Papadopoulou M. , Ji M. , Ji X. , Bloomer W.

Konu :

Pharmacology (medical), Cancer Research, Pharmacology, Toxicology, Oncology

Yıl :

2002

Ji M.

Özet Ek Bilgiler

Kaydet

Purpose. The antitumor effect and bone marrow toxicity of 5-fluorouracil (5FU) in combination with the hypoxia-selective cytotoxins NLCQ-1 or tirapazamine (TPZ) were investigated in vivo. Methods. Using appropriate intraperitoneal administration schedules for optimal synergistic interactions, the antitumor effect and the bone marrow toxicity of combinations of NLCQ-1 or TPZ and 5FU were determined in EMT6/BALB/c and SCCVII/C3H models in terms of dose modification factors (DMF) using the in vivo-in vitro clonogenic assay as endpoint. Bone marrow toxicity studies were performed in parallel using a modified CFU-GM assay. The antitumor efficacies of each combination treatment under optimal administration conditions were evaluated in the SCCVII/C3H model using also the tumor regrowth assay as endpoint. Results. A schedule-dependent and tumor-specific synergistic interaction was observed for NLCQ-1 plus 5FU and DMFs of 2.0–2.3 and 1.0 were obtained for the antitumor effect and bone marrow toxicity, respectively, in both tumor models. The antitumor effect of 5FU was slightly potentiated (DMF 1.2) by TPZ in the EMT6/BALB/c model but not in the SCCVII/C3H model when the in vivo-in vitro assay was used as the endpoint. Significant additional tumor regrowth delays (about 11 and 6 days for NLCQ-1 and TPZ, respectively) were observed, compared to the effect of 5FU alone, when an equitoxic dose of NLCQ-1 (10 mg/kg) or TPZ (23 mg/kg) was administered 1 h before 5FU (50 mg/kg) twice a day at 4-h intervals on days 0 and 9. Conclusions. These results corroborate the therapeutic advantage of combining hypoxia-selective cytotoxins such as NLCQ-1 and TPZ with chemotherapy.

DOI : 10.1007/s00280-002-0491-x

Anahtar Kelimeler : NLCQ-1 5-Fluorouracil Tirapazamine Potentiation Hypoxic cytotoxins

ISSN: 0344-5704 1432-0843
Sayı: 4
Cilt: 50
Sayfa: 291-298

6

LXY6090 – a novel manassantin A derivative – limits breast cancer growth through hypoxia-inducible factor-1 inhibition

Dergi Makalesi

Kaynak :

Directory of Open Access Journals (DOAJ)

Dergi:

OncoTargets and Therapy

Yazar(lar) :

Lai F , Liu Q , Liu X , Ji M , Xie P , Chen X

Yıl :

2016

Ji M

Özet Ek Bilgiler

Kaydet

Fangfang Lai,1 Qian Liu,2 Xiaoyu Liu,3 Ming Ji,1 Ping Xie,3 Xiaoguang Chen1 1Department of Pharmacology, State Key Laboratory of Bioactive Substances and Functions of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, 2Department of Pharmacology, National Institutes for Food and Drug Control, 3Department of Pharmacochemistry, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, People’s Republic of China Abstract: Hypoxia-inducible factor-1 (HIF-1) represents a novel antitumor target owing to its involvement in vital processes considered hallmarks of cancer phenotypes. Manassantin A (MA) derived from Saururus cernuus has been reported as a selective HIF-1 inhibitor. Herein, the structure of MA was optimized to achieve new derivatives with simple chemical properties while retaining its activity. LXY6090 was designed to replace the central tetrahydrofuran moiety of MA with a cyclopentane ring and was identified as a potent HIF-1 inhibitor with an IC50 value of 4.11 nM. It not only inhibited the activity of HIF-1 in breast cancer cells but also downregulated the protein level of HIF-1α, which depended on von Hippel–Lindau for proteasome degradation. The related biological evaluation showed that the activity of HIF-1 target genes, VEGF and IGF-2, was decreased by LXY6090 in breast cancer cell lines. LXY6090 presented potent antitumor activity in vitro. Furthermore, LXY6090 showed in vivo anticancer efficacy by decreasing the HIF-1α expression in nude mice bearing MX-1 tumor xenografts. In conclusion, our data provide a basis for the future development of the novel compound LXY6090 as a potential therapeutic agent for breast cancer. Keywords: hypoxia-inducible factor-1, manassantin A derivative, antitumor, breast cancer, LXY6090

Anahtar Kelimeler : Hypoxia-inducible factor 1 (HIF-1), Manassantin A derivative, anti-tumor, breast cancer, LXY6090

ISSN: 1178-6930
Cilt: 2016
Sayı: 1
Sayfa: 3829 - 3840

Sınıflandırma :

Biyotıp %98

Farmakoloji ve Toksikoloji %79

7

Endometrial carcinoma with yolk sac tumor-like differentiation and elevated serum ß-hCG: a case report and literature review

Dergi Makalesi

Kaynak :

Directory of Open Access Journals (DOAJ)

Dergi:

OncoTargets and Therapy

Yazar(lar) :

Ji M , Lu Y , Guo L , Feng F , Wan X , Xiang Y

Yıl :

2013

Ji M

Özet Ek Bilgiler

Kaydet

Mingliang Ji,1 Yan Lu,1 Lina Guo,2 Fengzhi Feng,1 Xirun Wan,1 Yang Xiang1 1Department of Obstetrics and Gynecology, 2Department of Pathology, Peking Union Medical College Hospital, Beijing, People's Republic of China Abstract: Endometrial carcinoma with a germ cell tumor component is a rare event. Here we report a uterine neoplasm with a unique combination of endometrioid adenocarcinoma and mixed germ cell malignant elements. A 28-year-old woman with abnormal vaginal bleeding, an abdominal mass, and elevated alfa-fetoprotein and beta-human chorionic gonadotropin (ß-hCG) levels had a history of biopsy of an omental mass and chemotherapy in another hospital one month before her referral to our department. Histologic examination of the mass removed from the omentum revealed an endometrioid adenocarcinoma with yolk sac tumor-like differentiation. Total abdominal hysterectomy, bilateral salpingo-oophorectomy, infracolic omentectomy, and removal of metastatic disease were then undertaken at our hospital. Postoperative chemotherapy was given. Eight months postoperatively, serum alfa-fetoprotein and ß-hCG rose again. Cases with primary yolk sac tumors of the endometrium or endometrial carcinoma with trophoblastic differentiation in the literature were reviewed. Keywords: endometrial carcinoma, yolk sac tumor, trophoblastic differentiation

ISSN: 1178-6930
Cilt: 2013
Sayı: default
Sayfa: 1515 - 1522

Sınıflandırma :

Jinekoloji %99

8

The Role of Methyltransferase NSD2 as a Potential Oncogene in Human Solid Tumors

Dergi Makalesi

Kaynak :

Directory of Open Access Journals (DOAJ)

Dergi:

OncoTargets and Therapy

Yazar(lar) :

Chen R , Chen Y , Zhao W , Fang C , Zhou W , Yang X , Ji M

Yıl :

2020

Ji M

Özet Ek Bilgiler

Kaydet

Rui Chen, Yan Chen, Weiqing Zhao, Cheng Fang, Wenjie Zhou, Xin Yang, Mei Ji Department of Oncology, The Third Affiliated Hospital of Soochow University, The First People’s Hospital of Changzhou, Changzhou 213003, People’s Republic of ChinaCorrespondence: Xin Yang; Mei Ji Email yangxindoctor@163.com; zlkjimei@163.comAbstract: Malignant solid tumors are the leading cause of death in humans, and epigenetic regulation plays a significant role in studying the mechanism of human solid tumors. Recently, histone lysine methylation has been demonstrated to be involved in the development of human solid tumors due to its epigenetic stability and some other advantages. The 90-kb protein methyltransferase nuclear receptor SET domain-containing 2 (NSD2) is a member of nuclear receptor SET domain-containing (NSD) protein lysine methyltransferase (KMT) family, which can cause epigenomic aberrations via altering the methylation states. Studies have shown that NSD2 is frequently over-expressed in multiple types of aggressive solid tumors, including breast cancer, renal cancer, prostate cancer, cervical cancer, and osteosarcoma, and such up-regulation has been linked to poor prognosis and recurrence. Further studies have identified that over-expression of NSD2 promotes cell proliferation, migration, invasion, and epithelial–mesenchymal transformation (EMT), suggesting its potential oncogenic role in solid tumors. Moreover, Gene Expression Profiling Interactive Analysis (GEPIA) was searched for validation of prognostic value of NSD2 in human solid tumors. However, the underlying specific mechanism remains unclear. In our present work, we summarized the latest advances in NSD2 expression and clinical applications in solid tumors, and our findings provided valuable insights into the targeted therapeutic regimens of solid tumors.Keywords: NSD2, proliferation, migration, invasion, EMT, oncogene

Anahtar Kelimeler : proliferation, migration, invasion, oncogene

ISSN: 1178-6930
Cilt: 13
Sayfa: 6837 - 6846

Sınıflandırma :

Biyotıp %100

Translasyon Sonrası Değişiklik %75

9

The Effects of Type 2 Diabetes and Postoperative Pneumonia on the Mortality in Inpatients with Surgery

Dergi Makalesi

Kaynak :

Directory of Open Access Journals (DOAJ)

Dergi:

Diabetes, Metabolic Syndrome and Obesity : Targets and Therapy

Yazar(lar) :

Ma C , Liu Q , Li M , Ji M , Zhang J , Zhang B , Yin FZ

Yıl :

2019

Ji M

Özet Ek Bilgiler

Kaydet

Chun-ming Ma, Qin Liu, Ming-li Li, Mei-jing Ji, Jian-dong Zhang, Bo-hua Zhang, Fu-Zai Yin Department of Endocrinology, The First Hospital of Qinhuangdao, Qinhuangdao, Hebei 066000, People’s Republic of ChinaCorrespondence: Fu-Zai YinDepartment of Endocrinology, The First Hospital of Qinhuangdao, Qinhuangdao, Hebei 066000, People’s Republic of ChinaTel +86-335-5908368Fax +86-335-3032042Email yinfuzai62@163.comObjective: The aim of the study was to explore the relationship between type 2 diabetes (T2DM) and postoperative pneumonia, and the effects of T2DM and postoperative pneumonia on the mortality in inpatients with surgery.Methods: A retrospective study was conducted on 43,174 inpatients with surgery in The First Hospital of Qinhuangdao. These patients were divided into four groups according to T2DM and postoperative pneumonia, Group A subjects without T2DM and postoperative pneumonia, Group B subjects with T2DM only, Group C subjects with postoperative pneumonia only and Group D subjects with T2DM and postoperative pneumonia. In-hospital mortality was collected.Results: The incidences of postoperative pneumonia were higher in patients with T2DM than patients without T2DM (T2DM 3.2% vs Non-diabetes 1.7%, χ2=36.219, P<0.001). The mortalities were 0.3% in Group A, 0.3% in Group B, 4.6% in Group C and 8.6% in Group D. In multiple logistic regression analysis, adjusted for sex, age, emergency admissions, coronary heart disease, heart failure, chronic kidney disease, hypoproteinemia, stroke and transient ischemic attack, the mortalities of Group C and Group D were 4.515 (95% CI: 2.779～7.336, P<0.001) times and 8.468 (95% CI: 3.567～20.099, P<0.001) times than the mortality of Group A.Conclusion: T2DM is susceptible to postoperative pneumonia. The mortality increased in patients with postoperative pneumonia. When patients with T2DM and postoperative pneumonia at the same time, the mortality increased further. In T2DM patients with postoperative pneumonia, perioperative management should be improved for patient safety.Keywords: type 2 diabetes, postoperative pneumonia, mortality

Anahtar Kelimeler : type 2 diabetes；postoperative pneumonia；mortality.

ISSN: 1178-7007
Cilt: 12
Sayfa: 2507 - 2513

10

TMEM119 promotes gastric cancer cell migration and invasion through STAT3 signaling pathway

Dergi Makalesi

Kaynak :

Directory of Open Access Journals (DOAJ)

Dergi:

OncoTargets and Therapy

Yazar(lar) :

Zheng P , Wang W , Ji M , Zhu Q , Feng Y , Zhou F , He Q

Yıl :

2018

Ji M

Özet Ek Bilgiler

Kaydet

Peifen Zheng, Weifeng Wang, Muxi Ji, Qin Zhu, Yuliang Feng, Feng Zhou, Qiaona He Department of Gastroenterology, Zhejiang Hospital, Hangzhou, People’s Republic of China Objective: TMEM119 is a member of transmembrane proteins family, which is abnormally expressed in human cancers and associated with tumorigenesis. In this study, we focused on the expression of TMEM119 and its role in cell invasion and migration in gastric cancer. Methods: Real-time polymerase chain reaction, Western blotting, and immunohistochemistry were performed to examine the expression of TMEM119 in gastric cancer tissues and cell lines. After transfection with TMEM119 siRNA or recombined TMEM119-expressing vector, the invasion and migration ability of MKN45 and SGC-7901 cells was measured by transwell assay. The expression of TMEM119, p-STAT3, STAT3, VEGF, MMP2, and MMP9 proteins in SGC-7901 and MKN45 cells treated with TMEM119 siRNA, TMEM119-expressing vector, or AG490 was measured by Western blotting. Results: We found that higher TMEM119 expression was found in gastric cancer tissues and cell lines and was associated with lower survival rate. TMEM119 knockdown inhibited SGC-7901 cell invasion and migration, along with the expression of p-STAT3, VEGF, MMP2, and MMP9. TMEM119 overexpression promoted MKN45 cell invasion and migration, along with the expression of p-STAT3, VEGF, MMP2, and MMP9. Additionally, AG490 treatment significantly corrected TMEM119-induced MKN45 cell migration and invasion and expression of p-STAT3, VEGF, MMP9, and MMP2 proteins. Conclusion: The results indicated that TMEM119 promotes gastric cancer cell migration and invasion through activation of STAT3 signaling pathway, and TMEM119 may therefore act as a novel therapeutic target for gastric cancer. Keywords: gastric cancer, TMEM119, migration, invasion, STAT3, VEGF, MMP2, MMP9

Anahtar Kelimeler : gastric cancer, TMEM119, migration, invasion, STAT3

ISSN: 1178-6930
Cilt: 11
Sayfa: 5835 - 5844

Sınıflandırma :

Biyotıp %100

Hücre Biyolojisi %94

11

Mupirocin resistance among MRSA surveillance isolates from neonatal intensive care unit under 10 year-search and destroy policy compared to

Dergi Makalesi

Kaynak :

Directory of Open Access Journals (DOAJ)

Dergi:

BMC Proceedings

Yazar(lar) :

Kim M-N , Kim H , An D , Ji M , Lee M , Kim A-R , Lee SJ

Yıl :

2011

Ji M

Piri Tam Metin Bulucu Ek Bilgiler

Kaydet

DOI : 10.1186/1753-6561-5-S6-P171

ISSN: 1753-6561
Cilt: 5
Sayı: Suppl 6
Sayfa: P171

Sınıflandırma :

Yaşam Bilimleri %95

Tıbbi Mikrobiyoloji %58

12

PTU-056 Loss of cathelicidin (LL-37) is associated with colorectal cancer progression

Konferans Bildirileri

Kaynak :

The BMJ

Dergi:

Colon and Anorectum

Yazar(lar) :

Ross J Porter , Graeme I Murray , Ji M Wang , Teizo Yoshimura , Mairi H McLean

Yıl :

2018

Ji M Wang

Ek Bilgiler

Kaydet

DOI : 10.1136/gutjnl-2018-bsgabstracts.397

Doküman etkinliği ;
Etkinlik adı:British Society of Gastroenterology, Annual General Meeting, 4–7 June 2018, Abstracts

Sınıflandırma :

Tıp ve Halk Sağlığı %99

Veterinerlik ve Veterinerlik Bilimi %100

13

Silencing CDR1as inhibits colorectal cancer progression through regulating microRNA-7

Dergi Makalesi

Kaynak :

Directory of Open Access Journals (DOAJ)

Dergi:

OncoTargets and Therapy

Yazar(lar) :

Tang W , Ji M , He G , Yang L , Niu Z , Jian M , Wei Y , Ren L , Xu J

Yıl :

2017

Ji M

Özet Ek Bilgiler

Kaydet

Wentao Tang,* Meiling Ji,* Guodong He,* Liangliang Yang, Zhengchuan Niu, Mi Jian, Ye Wei, Li Ren, Jianmin Xu Department of General Surgery, Zhongshan Hospital, Fudan University, Shanghai, People’s Republic of China *These authors contributed equally to this work Abstract: An increasing number of studies have demonstrated that circular RNAs (circRNAs) can regulate gene expression through interacting with microRNAs. In this study, we analyzed the expression of antisense to CDR1as in colorectal cancer (CRC). CDR1as had a higher expression in CRC tissues compared to adjacent, normal mucosa and was positively associated with tumor size, T stage, lymph node metastasis, and poor overall survival (OS). Downregulation of CDR1as suppressed CRC cell proliferation and invasion and increased microRNA-7 (miR-7) expression. Intriguingly, ectopic expression of miR-7 in CRC cells consistently inhibited proliferation and invasion, and the miR-7 inhibitor was able to rescue the function of CDR1as knockdown. Mechanistic studies demonstrated that CDR1as silencing suppressed EGFR and IGF-1R expression, which could be partially blocked by the miR-7 inhibitor. Finally, positive correlations between CDR1as expression and EGFR and IGF-1R expression were observed in CRC samples. Thus, given the importance of CDR1as in blocking miR-7 and positively regulating EGFR and IGF-1R, dysregulated CDR1as expression may play an important role in CRC progression. Keywords: CDR1as, colorectal cancer, microRNA-7, proliferation

Anahtar Kelimeler : CDR1as, Colorectal cancer, microRNA-7, proliferation

ISSN: 1178-6930
Cilt: 10
Sayfa: 2045 - 2056

Sınıflandırma :

Biyotıp %100

Hücre Biyolojisi %77

14

Long-Term Survival After Nasopharyngeal Carcinoma Treatment in a Local Prefecture-Level Hospital in Southern China

Dergi Makalesi

Kaynak :

Directory of Open Access Journals (DOAJ)

Dergi:

Cancer Management and Research

Yazar(lar) :

Du Y , Zhang W , Lei F , Yu X , Li Z , Liu X , Ni Y , Deng L , Ji M

Yıl :

2020

Ji M

Özet Ek Bilgiler

Kaydet

Yun Du,1,2 Wentong Zhang,3 Feng Lei,4 Xia Yu,1 Zhuming Li,1 Xiaodong Liu,1 Yanan Ni,1 Li Deng,4 Mingfang Ji1 1Cancer Research Institute of Zhongshan City, Zhongshan City People’s Hospital, Zhongshan 528400, People’s Republic of China; 2Department of Medical Epidemiology and Biostatistics, Karolinska Institutet, Solna, Stockholm, Sweden; 3Department of Chinese Medicine, Southern Medical University, Guangzhou, People’s Republic of China; 4Department of Radiotherapy, Zhongshan City People’s Hospital, Zhongshan, People’s Republic of ChinaCorrespondence: Mingfang JiCancer Research Institute of Zhongshan City, Zhongshan City People’s Hospital, No. 2. Sunwen Road East, Zhongshan 528400, Guangdong, People’s Republic of ChinaTel +86 135 3206 2222Fax +86 076488822698Email jmftbh@sina.comPurpose: NPC is a malignant and invasive tumor with the incidence rate of 19/100,000 per year in Zhongshan City, a prefecture city in southern China. Long-term survival analysis on intensity-modulated radiotherapy (IMRT)-based treatment in local prefecture-level hospitals have not been investigated. We aimed to evaluate the 5-year clinical outcomes and prognostic factors of NPC treated with IMRT in Zhongshan City People’s Hospital (ZSPH), a prefecture-level hospital in South China.Patients and Methods: The number of 149 newly diagnosed non-metastatic NPC cases treated with IMRT were included from Zhongshan City People’s Hospital between January 2010 and December 2011. The survival outcomes, treatment toxicities and prognostic factors were analyzed by Kaplan-Meier method and Cox proportional hazards model.Results: With a median follow-up period of 65 months for the cohort, the 5-year local recurrence-free survival (LRFS), regional recurrence-free survival (RRFS) and distant metastasis-free survival (DMFS) and overall survival (OS) were 86.80%, 94.80%, 86.10% and 80.50%, respectively. The 5-year OS rates were 100%, 95.2%, 87% and 67.2% for stage I, II, II and IVa-b, respectively (P=0.004). The 5-year LRFS rates were 97.2%, 96.0%, 90.4% and 72.0% for T1, T2, T3 and T4, respectively (P=0.001); the 5-year DMFS rates were 100% for T1, 96.8% for T2, 81.9% for T3 and 74.6% for T4 (P=0.022). A multivariate analysis revealed tumor stage as an independent prognostic factor for LRFS, DMFS and OS. No patients died from acute toxicities. Late toxicities were observed for 130 (87.2%) patients, and most late toxicities were graded I/II.Conclusion: NPC treatment effect in a prefecture-level hospital in South China was comparable to international results and toxicities were tolerable. Tumour stage was an independent prognostic factor for survival outcome. More NPC survival data from local and remote places are needed.Keywords: nasopharyngeal carcinoma, intensity-modulated, radiotherapy, prognosis, long-term survival results

Anahtar Kelimeler : nasopharyngeal carcinoma, intensity-modulated, radiotherapy, prognosis, long-term survival results.

ISSN: 1179-1322
Cilt: 12
Sayfa: 1329 - 1338

Sınıflandırma :

Biyotıp %62

Epidemiyoloji %59

Tropik ilaç %39

Tıp ve Halk Sağlığı %31

Kültürel çalışmalar %100

15

Novel lipophilic SN38 prodrug forming stable liposomes for colorectal carcinoma therapy

Dergi Makalesi

Kaynak :

Directory of Open Access Journals (DOAJ)

Dergi:

International Journal of Nanomedicine

Yazar(lar) :

Xing J , Zhang X , Wang Z , Zhang H , Chen P , Zhou G , Sun C , Gu N , Ji M

Yıl :

2019

Ji M

Özet Ek Bilgiler

Kaydet

Jing Xing,*,1,2 Xiquan Zhang,*,3 Zhe Wang,4 Huanqing Zhang,3 Peng Chen,1,2 Gaoxin Zhou,5 Chunlong Sun,6 Ning Gu,1,2 Min Ji1,21School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, People’s Republic of China; 2School of Biological Science and Medical Engineering and Collaborative Innovation Center of Suzhou Nano Science and Technology, Southeast University, Suzhou 215123, People’s Republic of China; 3Nanjing Institute of Pharmaceutical Research and Development, Chia-Tai Tianqing Pharmaceutical Group Co. Ltd, Nanjing 210023, People’s Republic of China; 4Emergency Department, The Second Affiliated Hospital of Nanjing Medical University, Nanjing 210029, People’s Republic of China; 5School of Biomedical Engineering, Shenzhen University, Shenzhen 518071, People’s Republic of China; 6College of Biological and Environmental Engineering, Binzhou University, Binzhou 256603, People’s Republic of China*These authors contributed equally to this workBackground: SN38 (7-ethyl-10-hydroxy camptothecin), as a potent metabolite of irinotecan, is highly efficacious in cancer treatment. However, the clinical utility of SN38 has been greatly limited due to its undesirable properties, such as poor solubility and low stability.Materials and methods: In order to overcome these weaknesses, moeixitecan, a lipophilic SN38 prodrug containing a SN-38, a trolox, a succinic acid linker, and a hexadecanol chain, was loaded into liposomal nanoparticles by ethanol injection method.Results: Experiments showed that the moeixitecan-loaded liposomal nanoparticles (MLP) with a diameter of 105.10±1.49 nm have a satisfactory drug loading rate (90.54±0.41%), high solubility and stability, and showed sustained release of SN38. Notably, MLP exhibited better antitumor activity against human colon adenocarcinoma cells than irinotecan, a FDA-approved drug for the treatment of advanced colorectal cancer. Furthermore, xenograft model results showed that MLP outperformed irinotecan in terms of pharmacokinetics, in vivo therapeutic efficacy and safety. Finally, we used molecular dynamic simulations to explore the association between the structure of MLP and the physical and functional properties of MLP, moeixitecan molecules in MLP folded themselves inside the hydrocarbon chain of the lipid bilayer, which led an increased acyl chain order of the lipid bilayer, and therefore enhanced the lactone ring stability protecting it from hydrolysis.Conclusion: Our MLP constructing strategy by liposome engineering technology may serve a promising universal approach for the effective and safe delivery of lipophilic prodrug.Keywords: SN38, lipophilic prodrug, liposomes, molecular dynamic simulations, cancer therapy

Anahtar Kelimeler : lipophilic prodrug, liposomes, Molecular dynamic simulations, Cancer therapy

Cilt: 14
Sayfa: 5201 - 5213

Sınıflandırma :

Biyotıp %96

Farmakoloji ve Toksikoloji %73

16

CAT3, a prodrug of 13a(S)-3-hydroxyl-6,7-dimethoxyphenanthro9,10-b-indolizidine, circumvents temozolomide-resistant glioblastoma via the Hedgehog signaling pathway, independently of O6-methylguanine DNA methyltransferase expression

Dergi Makalesi

Kaynak :

Directory of Open Access Journals (DOAJ)

Dergi:

OncoTargets and Therapy

Yazar(lar) :

Ji M , Wang L , Chen J , Xue N , Wang C , Lai F , Wang R , Yu SS , Jin J , Chen X

Yıl :

2018

Ji M

Özet Ek Bilgiler

Kaydet

Ming Ji,1 Liyuan Wang,1 Ju Chen,1 Nina Xue,2 Chunyang Wang,2 Fangfang Lai,2 Rubing Wang,1 Shishan Yu,1 Jing Jin,1,2 Xiaoguang Chen1,2 1State Key Laboratory of Bioactive Substances and Functions of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, People’s Republic of China; 2Beijing Key Laboratory of New Drug Mechanisms and Pharmacological Evaluation Study, Institute of Materia Medica, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing 100050, People’s Republic of China Purpose: Glioblastoma multiforme (GBM) is a malignant high-grade glioma with a poor clinical outcome. Temozolomide (TMZ) is the first-line GBM chemotherapy; however, patients commonly develop resistance to its effects. Materials and methods: We investigated the antitumor activity of CAT3 in TMZ-resistant glioblastoma cell lines U251/TMZ and T98G. Orthotopic and subcutaneous mice tumor models were used to investigate the effects of various treatment regimes. Results: We found that PF403, the active metabolite of CAT3, inhibited proliferation of both cell lines. PF403 repressed the Hedgehog signaling pathway in the U251/TMZ cell line, reduced O6-methylguanine DNA methyltransferase (MGMT) expression, and abolished the effects of the Shh pathway. Moreover, PF403 blocked the Hedgehog signaling pathway in T98G MGMT-expressing cells and downregulated the expression of MGMT. CAT3 suppressed growth in the U251/TMZ orthotopic and T98G subcutaneous xenograft tumor models in vivo. We also demonstrated that inhibition of the Hedgehog pathway by PF403 counteracted TMZ resistance and enhanced the antitumor activity of TMZ in vitro and in vivo. Conclusion: These results indicate that CAT3 is a potential therapeutic agent for TMZ-resistant GBM. Keywords: Gli inhibitor, chemotherapy, lomeguatrib, xenograft tumor model

Anahtar Kelimeler : Glioblastoma, Hedgehog, Gli inhibitor

ISSN: 1178-6930
Cilt: 11
Sayfa: 3671 - 3684

Sınıflandırma :

Biyotıp %100

Apoptoz %29

17

Controlled-releasing hydrogen sulfide donor based on dual-modal iron oxide nanoparticles protects myocardial tissue from ischemia–reperfusion injury

Dergi Makalesi

Kaynak :

Directory of Open Access Journals (DOAJ)

Dergi:

International Journal of Nanomedicine

Yazar(lar) :

Wang W , Liu H , Lu YT , Wang X , Zhang B , Cong S , Zhao Y , Ji M , Tao H , Wei L

Yıl :

2019

Ji M

Özet Ek Bilgiler

Kaydet

Wenshuo Wang,1,* Huan Liu,1,* Yuntao Lu,1,* Xiaole Wang,2,* Bohan Zhang,3 Shuo Cong,1 Yun Zhao,1 Minbiao Ji,3 Hongyue Tao,4 Lai Wei1,5 1Department of Cardiac Surgery, Zhongshan Hospital, Fudan University, Shanghai 200030, China; 2Department of Radiology, Second People’s Hospital of Nantong City, Nantong 226002, Jiangsu, China; 3State Key Laboratory of Surface Physics and Department of Physics, Fudan University, Shanghai 200433, China; 4Department of Radiology, Zhongshan Hospital, Fudan University, Shanghai 200040, China; 5Department of Cardiac Surgery, Shanghai Public Health Clincal Center, Shanghai 201508, China *These authors contributed equally to this work Background: Hydrogen sulfide (H2S) has shown promising therapeutic benefits in reversing a variety of pathophysiological processes in cardiovascular system, including myocardial ischemia–reperfusion (IR) injury. However, the achievement of controlled and sustained release of H2S has been a technical bottleneck that limits the clinical application of the gas molecule.Methods: The current study describes the development of mesoporous iron oxide nanoparticles (MIONs) which were loaded with diallyl trisulfide (DATS), a H2S donor compound, and calibrated by stimulated Raman scattering/transient absorption.Results: The synthesized MIONs were characterized with excellent mesoporosity and a narrow size distribution, which enabled them to slow down the release of H2S to a suitable rate and prolong the plateau period. The controlled-release feature of DATS-MIONs resulted in little adverse effect both in vitro and in vivo, and their protective effect on the heart tissue that underwent IR injury was observed in the mouse model of myocardial ischemia. The rapid biodegradation of DATS-MIONs was induced by Kupffer cells, which were specialized macrophages located in the liver and caused limited hepatic metabolic burden.Conclusion: The sustained-release pattern and excellent biocompatibility make DATS-MIONs a promising H2S donor for research and medical purposes. Keywords: steady release, porous structure, biocompatibility, biodegeneration

Anahtar Kelimeler : steady release, porous structure, biocompatibility, biodegeneration.

Cilt: 14
Sayfa: 875 - 888

Sınıflandırma :

Kimya %35

Nükleer Kimya %65

Biyotıp %30

Metabolik Hastalıklar %86

18

Tumor deposit is a poor prognostic indicator in patients who underwent simultaneous resection for synchronous colorectal liver metastases

Dergi Makalesi

Kaynak :

Directory of Open Access Journals (DOAJ)

Dergi:

OncoTargets and Therapy

Yazar(lar) :

Lin Q , Wei Y , Ren L , Zhong Y , Qin C , Zheng P , Xu P , Zhu D , Ji M , Xu J

Yıl :

2015

Ji M

Özet Ek Bilgiler

Kaydet

Qi Lin,# Ye Wei,# Li Ren,# Yunshi Zhong,# Chunzhi Qin, Peng Zheng, Pingping Xu, Dexiang Zhu, Meiling Ji, Jianmin XuDepartment of General Surgery, Zhongshan Hospital, Fudan University, Shanghai, People's Republic of China#These authors contributed equally to this workBackground: Tumor deposits are one of the important influencing factors among the different editions of Tumor, Node, Metastasis classification. Incidence and prognosis of tumor deposits in stage I, II, and III colorectal cancer patients has been explored. The aim of this study was to determine the prognostic value of tumor deposits in stage IV colorectal cancer patients who underwent simultaneous resection for synchronous colorectal liver metastases (SCRLM).Methods: Clinicopathological and outcome data of 146 consecutive SCRLM patients who underwent simultaneous R0 resection between July 2003 and July 2013 were collected from our prospectively established SCRLM database. The prognostic value of tumor deposits was evaluated by Kaplan–Meier and Cox regression analysis.Results: Tumor deposits were detected in 41.8% (61/146) of these SCRLM patients. Tumor deposits were significantly correlated with lymph node metastasis and nerve invasion of the primary tumors (P=0.002, P=0.041; respectively). The Kaplan–Meier survival analysis revealed that the overall survival (OS) and disease-free survival (DFS) of SCRLM patients with tumor deposits were significantly poorer than those with no tumor deposits (P=0.039, P=0.001; respectively). And with multivariate analysis, we found that positive tumor deposits were significantly associated with shorter DFS independent of lymph node status (P=0.002). Subgroup analysis found that of the 57 SCRLM patients with negative lymph node status, the OS and DFS of patients with positive tumor deposits were significantly shorter than those with negative tumor deposits (P=0.002 and P=0.031, respectively). Of the 89 patients with positive lymph node status, the OS of patients with tumor deposits was not significantly different than those without tumor deposits (P=0.965); however, the DFS of patients with tumor deposits was significantly shorter than those with no tumor deposits (P=0.034).Conclusion: Tumor deposits may be an independent adverse prognostic factor in SCRLM patients who underwent simultaneous R0 resection.Keywords: tumor deposits, synchronous colorectal liver metastases, prognostic factors

ISSN: 1178-6930
Cilt: 2015
Sayı: default
Sayfa: 233 - 240

Sınıflandırma :

Cerrahi Onkoloji %42

Kanser Araştırmaları %40

19

CDKL1 promotes tumor proliferation and invasion in colorectal cancer

Dergi Makalesi

Kaynak :

Directory of Open Access Journals (DOAJ)

Dergi:

OncoTargets and Therapy

Yazar(lar) :

Qin C , Ren L , Ji M , Lv S , Wei Y , Zhu D , Lin Q , Xu P , Chang W , Xu J

Yıl :

2017

Ji M

Özet Ek Bilgiler

Kaydet

Chunzhi Qin,1,* Li Ren,1,* Meiling Ji,1,* Shixu Lv,2,* Ye Wei,1 Dexiang Zhu,1 Qi Lin,1 Pingping Xu,1 Wenju Chang,1 Jianmin Xu1 1Department of General Surgery, Zhongshan Hospital, Fudan University, Shanghai, 2Department of Surgical Oncology, First Affiliated Hospital of Wenzhou Medical University, Wenzhou, People’s Republic of China *These authors contributed equally to this work Background: CDKL1 is a member of the cell division cycle 2 (CDC2)-related serine threonine protein kinase family and is overexpressed in malignant tumors such as melanoma, breast cancer, and gastric cancer.Objective: This study aimed to evaluate whether CDKL1 can serve as a potential molecular target for colorectal cancer therapy.Materials and methods: Expression of CDKL1 in colorectal cancer tissues and cell lines was measured by immunohistochemistry and Western blot, respectively. To investigate the role of CDKL1 in colorectal cancer, CDKL1-small hairpin RNA-expressing lentivirus was constructed and infected into HCT116 and Caco2 cells. The effects of RNA interference (RNAi)-mediated CDKL1 downregulation on cell proliferation and invasion were assessed by CCK-8, colony formation, transwell, and tumorigenicity assays in nude mice. The effects of CDKL1 downregulation on cell cycle and apoptosis were analyzed by flow cytometry. Furthermore, microarray method and data analysis elucidated the molecular mechanisms underlying the phenomenon.Results: CDKL1 protein was overexpressed in colorectal cancer tissues compared with paired normal tissues. Knockdown of CDKL1 in HCT116 and Caco2 significantly inhibited cell growth, colony formation ability, tumor invasion, and G1–S phase transition of the cell cycle. The knockdown of CDKL1 stimulated the upregulation of p15 and retinoblastoma protein.Conclusion: CDKL1 plays a vital role in tumor proliferation and invasion in colorectal cancer in vitro and in vivo and, thus, may be considered as a valuable target for therapeutic intervention. Keywords: CDKL1, cell cycle, protein kinases, colorectal cancer

Anahtar Kelimeler : CDKL1, Cell cycle, Protein Kinases, Colorectal cancer

ISSN: 1178-6930
Cilt: 10
Sayfa: 1613 - 1624

Sınıflandırma :

Biyotıp %100

Hücre Biyolojisi %61

20

PRKD2 Promotes Progression and Chemoresistance of AML via Regulating Notch1 Pathway

Dergi Makalesi

Kaynak :

Directory of Open Access Journals (DOAJ)

Dergi:

OncoTargets and Therapy

Yazar(lar) :

Liu Q , Li W , Zhou Y , Jian J , Han S , Liu C , Li W , Zhu X , Ma D , Ji M , Ji C

Yıl :

2019

Ji M

Özet Ek Bilgiler

Kaydet

Qian Liu,1,2 Wei Li,1 Ying Zhou,1 Jimo Jian,1,3 Shijie Han,4 Chao Liu,5 Wei Li,1 Xunxun Zhu,6 Daoxin Ma,1,7 Min Ji,1 Chunyan Ji1 1Department of Hematology, Qilu Hospital, Shandong University, Jinan 250012, People’s Republic of China; 2Department of Pain, Qilu Hospital, Shandong University, Jinan 250012, People’s Republic of China; 3Department of Hematology, Qilu Hospital, Shandong University (Qingdao), Qingdao 266000, People’s Republic of China; 4Department of Orthopedics, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, People’s Republic of China; 5Department of Oral and Maxillofacial Surgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250012, People’s Republic of China; 6Department of Hematology, Tengzhou Central People’s Hospital, Tengzhou 277599, People’s Republic of China; 7Shandong Provincial Key Laboratory of Immunohematology, Qilu Hospital, Shandong University, Jinan 250012, People’s Republic of ChinaCorrespondence: Min JiDepartment of Hematology, Qilu Hospital, Shandong University, West Wenhua Road, Jinan 250012, People’s Republic of ChinaEmail jimin@sdu.edu.cnIntroduction: Progression and chemoresistance of acute myeloid leukemia (AML) contribute to most of the treatment failure. Notch pathway has been proven to be involved in many biological processes and diseases, especially AML. In this study, we aimed to explore genes correlated with Notch1 pathway in AML and determine their roles in the regulation of AML progression and chemoresistance.Methods: TCGA database was used to explore Notch1 associated genes. Kaplan–Meier survival analysis was performed to evaluate the prognostic significance of genes. Quantitative RT-PCR (qRT-PCR) and Western blot were performed to examine the expression of genes. The expression of PRKD2 was up-regulated or knocked down in AML cell lines by lentivirus or siRNAs. CCK-8 and flow cytometry were used to analyze the effect of PRKD2 on cell proliferation and chemoresistance.Results: Based on TCGA database, PRKD2 was found to be positively correlated with Notch1 expression, cytogenetic risk status and poorer prognosis in AML. Moreover, the expression level of PRKD2 was higher in AML chemo-resistant cells than in chemo-sensitive cells. Functionally, knockdown of PRKD2-induced apoptosis and increased chemosensitivity of AML cells. PRKD2 overexpression promoted proliferation and chemoresistance of AML cells. Furthermore, we found PRKD2 could regulate Notch1 pathway. Besides, high PRKD2 expression was correlated with higher risk group of AML patients which indicated that PRKD2 was an independent prognostic marker for AML.Conclusion: Taken together, our results showed that PRKD2 could promote the proliferation and chemoresistance of AML cells by regulating Notch1 pathway. The study broadened our insights into the underlying mechanisms in chemoresistance and proliferation of AML, and provided a new prognostic marker and treatment target for AML.Keywords: PRDK2, AML, progression, chemoresistance, Notch1

Anahtar Kelimeler : prdk2, progression, chemoresistance, notch1

ISSN: 1178-6930
Cilt: 12
Sayfa: 10931 - 10941

Sınıflandırma :

Biyotıp %70

Tropik ilaç %39

Hematoloji %34

Tıp ve Halk Sağlığı %26

Adli Psikiyatri %100

21

Differences in clinical characteristics and mutational pattern between synchronous and metachronous colorectal liver metastases

Dergi Makalesi

Kaynak :

Directory of Open Access Journals (DOAJ)

Dergi:

Cancer Management and Research

Yazar(lar) :

Zheng P , Ren L , Feng Q , Zhu D , Chang W , He G , Ji M , Jian M , Lin Q , Yi T , Wei Y , Xu J

Yıl :

2018

Ji M

Özet Ek Bilgiler

Kaydet

Peng Zheng,* Li Ren,* Qingyang Feng,* Dexiang Zhu,* Wenju Chang, Guodong He, Meiling Ji, Mi Jian, Qi Lin, Tuo Yi, Ye Wei, Jianmin Xu Department of General Surgery, Zhongshan Hospital, Fudan University, Shanghai, People’s Republic of China *These authors contributed equally to this work Purpose: To investigate differences in clinical characteristics and mutational patterns between synchronous and metachronous colorectal liver metastases (CLMs). Patients and methods: From June 2008 to December 2014, patients with RAS wild-type CLMs treated at Zhongshan Hospital, Fudan University were included. DNA extracted from formalin-fixed paraffin-embedded tissue of primary tumors was sequenced with next-generation sequencing for single-nucleotide polymorphism of 96 genes according to custom panel. Mutations were compared between synchronous and metachronous liver metastases and correlated with clinical characteristics. Results: A total of 161 patients were included: 93 patients with synchronous CLM and 68 patients with metachronous CLM. Patients with metachronous CLM were obviously elder. For pathology of primary tumors, synchronous CLMs were larger in size, poorly differentiated, and more frequently local advanced and lymph node positive. For evaluation of liver metastases, synchronous CLM had more and larger metastatic lesions. The median number of mutations in synchronous CLMs was significantly higher than in metachronous group (22 vs. 18, p<0.001). EGFR rs2227983 is the most prevalent mutation in both groups and only a part of prevalent mutations is shared in both groups. Prevalent mutations were correlated with many clinical characteristics. EGFR rs2227983, RBMXL3 rs12399211, and PTCH1 rs357564 were prognostic for latency of metachronous CLM. Conclusion: Clinically, synchronous CLMs, compared with metachronous CLMs, were younger and showed heavier tumor burden for both primaries and liver metastases. Genetically, we identified different mutational patterns between synchronous and metachronous CLMs and several correlations between mutations and clinical characteristics. Further researches were needed to confirm these potential key mutations of CLMs. Keywords: colorectal liver metastases, next-generation sequencing, metachronous, synchronous

Anahtar Kelimeler : colorectal liver metastases, next-generation sequencing, metachronous, synchronous

ISSN: 1179-1322
Cilt: 10
Sayfa: 2871 - 2881

22

Low tumor purity is associated with poor prognosis, heavy mutation burden, and intense immune phenotype in colon cancer

Dergi Makalesi

Kaynak :

Directory of Open Access Journals (DOAJ)

Dergi:

Cancer Management and Research

Yazar(lar) :

Mao Y , Feng Q , Zheng P , Yang L , Liu T , Xu Y , Zhu D , Chang W , Ji M , Ren L , Wei Y , He G , Xu J

Yıl :

2018

Ji M

Özet Ek Bilgiler

Kaydet

Yihao Mao,1,* Qingyang Feng,1,2,* Peng Zheng,1,* Liangliang Yang,1,* Tianyu Liu,1 Yuqiu Xu,1 Dexiang Zhu,1,2 Wenju Chang,1,2 Meiling Ji,1,2 Li Ren,1,2 Ye Wei,1,2 Guodong He,1,2 Jianmin Xu1,2 1Department of General Surgery, Zhongshan Hospital, Fudan University, Shanghai 200030, China; 2Shanghai Engineering Research Center of Colorectal Cancer Minimally Invasive, Shanghai 200030, China *These authors contributed equally to this work Purpose: Tumor purity is defined as the proportion of cancer cells in the tumor tissue. The impact of tumor purity on colon cancer (CC) prognosis, genetic profile, and microenvironment has not been thoroughly accessed.Materials and methods: Clinical and transcriptomic data from three public datasets, GSE17536/17537, GSE39582, and TCGA, were retrospectively collected (n=1,248). Tumor purity of each sample was inferred by a computational method based on transcriptomic data. Survival-related analyses were performed on microarray dataset containing GSE17536/17537 and GSE39582 (n=794), whereas TCGA dataset was utilized for subsequent genomic analysis (n=454).Results: Right-sided CC patients showed a significantly lower tumor purity. Low purity CC conferred worse survival, and tumor purity was identified as an independent prognostic factor. Moreover, high tumor purity CC patients benefited more from adjuvant chemotherapy. Subsequent genomic analysis found that the mutation burden was negatively associated with tumor purity, with only APC and KRAS significantly more mutated in high purity CC. However, no somatic copy number alteration event was correlated with tumor purity. Furthermore, immune-related pathways and immunotherapy-associated markers (programmed cell death protein 1 [PD-1], programmed death-ligand 1 [PD-L1], cytotoxic T-lymphocyte-associated protein 4 [CTLA-4], Lymphocyte-activation gene 3 [LAG-3] and T-cell immunoglobulin and mucin-domain containing-3 [TIM-3]) were highly enriched in low purity samples. Notably, the relative proportion of M2 macrophages and neutrophils, which indicated worse survival in CC, was negatively associated with tumor purity.Conclusion: Tumor purity exhibited potential value for CC prognostic stratification as well as adjuvant chemotherapy benefit prediction. The relative worse survival in low purity CC may attribute to higher mutation frequency in key pathways and purity-related microenvironmental changing. Keywords: adjuvant chemotherapy, colon cancer, prognosis, tumor microenvironment, tumor purity

Anahtar Kelimeler : adjuvant chemotherapy, colon cancer, prognosis, tumor microenvironment, tumor purity

ISSN: 1179-1322
Cilt: 10
Sayfa: 3569 - 3577

Sınıflandırma :

Biyotıp %100

Metabolik Hastalıklar %42

Biyotıp %29

23

Drug-free mannosylated liposomes inhibit tumor growth by promoting the polarization of tumor-associated macrophages

Dergi Makalesi

Kaynak :

Directory of Open Access Journals (DOAJ)

Dergi:

International Journal of Nanomedicine

Yazar(lar) :

Ye J , Yang Y , Dong W , Gao Y , Meng Y , Wang H , Li L , Jin J , Ji M , Xia X , Chen X , Jin Y , Liu Y

Yıl :

2019

Ji M

Özet Ek Bilgiler

Kaydet

Jun Ye,1–3 Yanfang Yang,1,2 Wujun Dong,1,2 Yue Gao,1,2 Yingying Meng,1,2 Hongliang Wang,1,2 Lin Li,1,2 Jing Jin,1 Ming Ji,1 Xuejun Xia,1,2 Xiaoguang Chen,1 Yiqun Jin,3 Yuling Liu1,21State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, People’s Republic of China; 2Beijing Key Laboratory of Drug Delivery Technology and Novel Formulation, Institute of Materia Medica, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100050, People’s Republic of China; 3Research and Development Department, Beijing Wehand-bio Pharmaceutical Co. Ltd, Beijing, 102600, People’s Republic of ChinaBackground: Tumor-associated macrophages (TAMs) are critical in tumor progression and metastasis. Selective targeting of TAMs holds great potential to ameliorate the immunosuppressive tumor microenvironment and enhance the efficacy of antitumor therapy. Various liposomes have been developed to target TAMs via cell-specific surface receptors either to deplete or re-educate TAMs. Since immuno-stimulation often initiates with the interaction of nanocarriers with the innate immunity cells such as macrophages, the intrinsic impact of drug-free liposomes on macrophage activation and polarization via cell interaction is one of the most critical issues in nanomedicine for promoting effective immunotherapy.Methods: In this study, conventional bare liposomes, PEGylated liposomes, and mannosylated liposomes were developed and the cytotoxicity, cellular internalization, immunostimulatory activity, targeting efficiency, antitumor efficacy, and mechanism were evaluated in vitro and in vivo.Results: All liposomes displayed an ideal particle size, good biocompatibility, and controlled release behavior. Mannosylated liposomes exhibited superior in vitro cellular internalization and tumor spheroid penetration with the aid of the mannose receptor-mediated TAMs-targeting effects. In particular, mannosylated liposomes promoted the polarization of both M0 and M2 to the M1 phenotype by enhancing the expression ratio of CD86/CD206 in vitro. Of note, mannosylated liposomes could inhibit G422 glioma tumor growth, which may be attributed to the polarization of TAMs, as evidenced by the reduction in expression level of the TAMs surface marker.Conclusion: These results indicate the potential value of mannosylated liposomes in the design of a rational delivery system to enhance the antitumor immune efficacy of immunomodulators by inducing a shift from the M2 to the M1 phenotype.Keywords: liposomes, cancer immunotherapy, tumor-associated macrophages, mannose receptor, drug delivery

Anahtar Kelimeler : liposomes, cancer immunotherapy, tumor-associated macrophages, mannose receptor, drug delivery

Cilt: 14
Sayfa: 3203 - 3220

24

Pharmacokinetics of DA-6034, an agent for inflammatory bowel disease, in rats and dogs: Contribution of intestinal first-pass effect to low bioavailability in rats

Dergi Makalesi

Kaynak :

Science Direct

Dergi:

European Journal of Pharmaceutical Sciences

Yayıncı:

Elsevier

Yazar(lar) :

Hye J. Chung , Young H. Choi , Hye D. Choi , Ji M. Jang , Hyun J. Shim , Moohi Yoo , Jong W. Kwon , Myung G. Lee

Yıl :

2006

Ji M. Jang

Özet Ek Bilgiler

Kaydet

The pharmacokinetics of DA-6034 in rats and dogs and first-pass effect in rats were examined. After intravenous administration, the dose-normalized AUC0–∞ values at 25 and 50 mg/kg were significantly smaller than that at 10 mg/kg. This could be due to significantly slower Clr values than that at 10 mg/kg, possibly due to saturated renal secretion at doses of 25 and 50 mg/kg. After oral administration, the dose-normalized AUC0–12 h values at 50 and 100 mg/kg were significantly smaller than that at 25 mg/kg, possibly due to poor water solubility of the drug. The low F-value (approximately 0.136%) of DA-6034 at a dose of 50 mg/kg in rats could be due to considerable intestinal first-pass effect (approximately 69% of oral dose) and unabsorbed fraction from the gastrointestinal tract (approximately 30.5%). The effect of cola beverage, cimetidine, or omeprazole on the AUC0–24 h of DA-6034 was almost negligible in rats. Pharmacokinetic parameters of DA-6034 after intravenous and oral administration at various doses were dose-independent in dogs. DA-6034 was not accumulated in rats and dogs after consecutive 7 and 28 days oral administration, respectively. The stability, blood partition, and protein binding of DA-6034 were also discussed.

DOI : 10.1016/j.ejps.2005.11.008

Anahtar Kelimeler : DA-6034, Dose-dependent AUC (or AUC0u201312 h), Intestinal first-pass effects, Unabsorbed fractions, Rats

ISSN: 0928-0987
Sayı: 4
Cilt: 27
Sayfa: 363-374

Sınıflandırma :

Biyotıp %100

Farmakoloji ve Toksikoloji %96

25

Protective effects of triamcinolone acetonide upon the upregulation and phosphorylation of GAP 43 in an animal model of retinopathy of prematurity

Dergi Makalesi

Kaynak :

Wiley Online Library

Dergi:

Acta Ophthalmologica

Yazar(lar) :

In Y. Chung , Young H. Kim , Jong M. Park , Seong W. Seo , Wan S. Choi , Gyeong J. Cho , Ji M. Yoo

Konu :

Ophthalmology, General Medicine

Yıl :

2010

Ji M. Yoo

Ek Bilgiler

Kaydet

DOI : 10.1111/j.1755-3768.2010.01951.x

ISSN: 1755-375X
Sayı: 6
Cilt: 88
Sayfa: e217-e221

26

Alpha-synuclein fibrils recruit TBK1 and OPTN to lysosomal damage sites and induce autophagy in microglial cells.

Dergi Makalesi

Kaynak :

PubMed

Dergi:

J. Cell. Sci.

Yazar(lar) :

Claudio Bussi , Javier M Peralta Ramos , Daniela S Arroyo , Jose I Gallea , Paolo Ronchi , Androniki Kolovou , Ji M Wang , Oliver Florey , Maria S Celej , Yannick Schwab , Nicholas T Ktistakis , Pablo Iribarren

Yıl :

2018

Ji M Wang

Piri Tam Metin Bulucu Özet Ek Bilgiler

Kaydet

Autophagic dysfunction and protein aggregation have been linked to several neurodegenerative disorders, but the exact mechanisms and causal connections are not clear and most previous work was done in neurons and not in microglial cells. Here, we report that exogenous fibrillary, but not monomeric, alpha-synuclein (AS, also known as SNCA) induces autophagy in microglial cells. We extensively studied the dynamics of this response using both live-cell imaging and correlative light-electron microscopy (CLEM), and found that it correlates with lysosomal damage and is characterised by the recruitment of the selective autophagy-associated proteins TANK-binding kinase 1 (TBK1) and optineurin (OPTN) to ubiquitylated lysosomes. In addition, we observed that LC3 (MAP1LC3B) recruitment to damaged lysosomes was dependent on TBK1 activity. In these fibrillar AS-treated cells, autophagy inhibition impairs mitochondrial function and leads to microglial cell death. Our results suggest that microglial autophagy is induced in response to lysosomal damage caused by persistent accumulation of AS fibrils. Importantly, triggering of the autophagic response appears to be an attempt at lysosomal quality control and not for engulfment of fibrillar AS.This article has an associated First Person interview with the first author of the paper.

DOI : 10.1242/jcs.226241

Anahtar Kelimeler : Alpha-synuclein, Autophagy, Cell death, Lysosomes, Microglia

Sınıflandırma :

Biyotıp %100

Hücre Biyolojisi %47

Apoptoz %28

Nörokimya %21

27

Biodegradation of benzene and its derivatives by a psychrotolerant and moderately haloalkaliphilic Planococcus sp. strain ZD22

Dergi Makalesi

Kaynak :

Science Direct

Dergi:

Research in Microbiology

Yayıncı:

Elsevier

Yazar(lar) :

He Li , Yu H. Liu , Na Luo , Xiao Y. Zhang , Tian G. Luan , Ji M. Hu , Zhuo Y. Wang , Pei C. Wu , Min J. Chen , Jia Q. Lu

Yıl :

2006

Ji M. Hu

Özet Ek Bilgiler

Kaydet

The potential for biodegradation of aromatic hydrocarbons simultaneously at low temperatures and under saline and alkaline conditions is not well understood, but such biodegradation would be useful for remediation of polluted sites. A psychrotolerant, moderately haloalkaliphilic pure culture using benzene as a sole source of carbon and energy was isolated by selective enrichment from alkaline and saline soils in the vicinity of the Daqing oil field in China. An analysis of the 16S rDNA gene sequence and morphological and physiological characteristics showed that this strain is a member of the genus Planococcus, and it was designated as strain ZD22. Strain ZD22 could grow at temperatures between 2 and 36 °C (pH 7.5–11) and salt concentrations from 0.5 to 25%. Its optimal conditions for biodegradation of benzene were 20 °C (pH 9.5) and 10% salt concentration. Strain ZD22 not only utilized benzene, toluene, ethylbenzene and o-xylene, but also degraded chlorobenzene, bromobenzene, iodobenzene and fluorobenzene. The kinetic model of strain ZD22 for benzene was solved to obtain μmax=0.34 h−1, Ks=0.041 mM, n=1.21, Sm=10.2 mM. To our knowledge, this is the first report of biodegradation of benzene and its derivatives simultaneously under multiple extreme conditions.

DOI : 10.1016/j.resmic.2006.01.002

ISSN: 0923-2508
Sayı: 7
Cilt: 157
Sayfa: 629-636

Sınıflandırma :

Yaşam Bilimleri %94

Mikoloji %59

28

A nomogram for endoscopic screening in a high esophageal squamous cell cancer risk area: results from a population-based study

Dergi Makalesi

Kaynak :

Directory of Open Access Journals (DOAJ)

Dergi:

Cancer Management and Research

Yazar(lar) :

Xing J , Min L , Zhang H , Li P , Li W , Lv F , Wang Y , Zhang Z , Li H , Guo Q , Wang S , Zhao Y , Wang J , Shi X , Wang A , Zhu S , Ji M , Wu Y , Zhang S

Yıl :

2019

Ji M

Özet Ek Bilgiler

Kaydet

Jie Xing,1 Li Min,1 Hao Zhang,2 Peng Li,1 Wei Li,1 Fujin Lv,1 Yongjun Wang,1 Zheng Zhang,1 Hengcun Li,1 Qingdong Guo,1 Siyi Wang,1 Yu Zhao,1 Junmin Wang,3 Xiaoyan Shi,4 Anxin Wang,5 Shengtao Zhu,1 Ming Ji,1 Yongdong Wu,1 Shutian Zhang1 On behalf of the NCECS Study Group 1Department of Gastroenterology, Beijing Friendship Hospital, Capital Medical University, National Clinical Research Center for Digestive Disease, Beijing Digestive Disease Center, Beijing Key Laboratory for Precancerous Lesion of Digestive Disease, Beijing 100050, People’s Republic of China; 2Department of Gastroenterology, Jizhong Energy Fengfeng Group Hospital, Handan, Hebei 056200, People’s Republic of China; 3Department of Gastroenterology, Handan Central Hospital, Handan, Hebei 056001, People’s Republic of China; 4Department of Pathology, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, People’s Republic of China; 5Department of Epidemiology, Beijing Tiantan Hospital, Capital Medical University, Beijing 100029, People’s Republic of China Background: Endoscopy is the main approach used for esophageal squamous cell carcinoma (ESCC) screening, especially in high-risk areas. However, little consensus has been achieved in recent ESCC screening programs, and endoscopists have selected patients only by age and family history. Patients and methods: To generate a proper strategy for selecting an eligible population for endoscopic screening based on demographic factors, lifestyle, and eating habits, a total of 7,830 residents in an area with a high risk of ESCC were recruited for this study. All participants underwent endoscopic examinations that were conducted by experienced endoscopists. Risk factors for ESCC and other lesions were selected by univariate and multivariate logistic regressions. A nomogram for the prediction of ESCC and premalignant lesions was constructed, which included information on age, sex, occupation, labor intensity, income, and mining exposure. Receiver operating characteristic (ROC) curve analysis was performed to present the predictive accuracy of the nomograms. Results: The area under the curve (95% CI) was 0.749 (0.711–0.788) for this nomogram. By applying this nomogram, we could exclude 60% (4704/7830) of patients before endoscopy screening and detect all ESCC cases as well as most esophageal lesions in the remaining population. Conclusion: In conclusion, we provided a ready-to-use preclinical tool with the potential to select eligible people with high risk of ESCC for endoscopy screening. Keywords: nomogram, endoscopy screening, ESCC, population-based study

Anahtar Kelimeler : Nomogram, Endoscopy Screening, Population-based Study

ISSN: 1179-1322
Cilt: 11
Sayfa: 431 - 442

Sınıflandırma :

Bulaşıcı Hastalıklar %30

Gastroenteroloji %22

29

Colonic epithelial cathelicidin ( LL ‐37) expression intensity is associated with progression of colorectal cancer and presence of CD8 + T cell infiltrate

Dergi Makalesi

Kaynak :

Wiley Online Library

Dergi:

The Journal of Pathology: Clinical Research

Yazar(lar) :

Ross J Porter , Graeme I Murray , Abdo Alnabulsi , Matthew P Humphries , Jacqueline A James , Manuel Saltou2010Tellez , Stephanie G Craig , Ji M Wang , Teizo Yoshimura , Mairi H McLean

Yıl :

2021

Ji M Wang

Özet Ek Bilgiler

Kaydet

Colorectal cancer (CRC) remains a leading cause of cancer mortality. Here, we define the colonic epithelial expression of cathelicidin (LL‐37) in CRC. Cathelicidin exerts pleotropic effects including anti‐microbial and immunoregulatory functions. Genetic knockout of cathelicidin led to increased size and number of colorectal tumours in the azoxymethane‐induced murine model of CRC. We aimed to translate this to human disease. The expression of LL‐37 in a large (n = 650) fully characterised cohort of treatment‐naïve primary human colorectal tumours and 50 matched normal mucosa samples with associated clinical and pathological data (patient age, gender, tumour site, tumour stage [UICC], presence or absence of extra‐mural vascular invasion, tumour differentiation, mismatch repair protein status, and survival to 18 years) was assessed by immunohistochemistry. The biological consequences of LL‐37 expression on the epithelial barrier and immune cell phenotype were assessed using targeted quantitative PCR gene expression of epithelial permeability (CLDN2, CLDN4, OCLN, CDH1, and TJP1) and cytokine (IL‐1β, IL‐18, IL‐33, IL‐10, IL‐22, and IL‐27) genes in a human colon organoid model, and CD3+, CD4+, and CD8+ lymphocyte phenotyping by immunohistochemistry, respectively. Our data reveal that loss of cathelicidin is associated with human CRC progression, with a switch in expression intensity an early feature of CRC. LL‐37 expression intensity is associated with CD8+ T cell infiltrate, influenced by tumour characteristics including mismatch repair protein status. There was no effect on epithelial barrier gene expression. These data offer novel insights into the contribution of LL‐37 to the pathogenesis of CRC and as a therapeutic molecule.

DOI : 10.1002/cjp2.222

ISSN: 2056-4538
Sayı: 5
Cilt: 7
Sayfa: 495-506

Sınıflandırma :

Biyotıp %100

İnsan Genetiği %99

30

Subacute toxicities and toxicokinetics of DA-8159, a new phosphodiesterase type V inhibitor, after single and 4-Week repeated oral administration in rats

Dergi Makalesi

Kaynak :

Wiley Online Library

Dergi:

Biopharmaceutics & Drug Disposition

Yazar(lar) :

Hyun J. Shim , Yu C. Kim , Ji M. Jang , Kyung J. Park , Dong H. Kim , Kyung K. Kang , Byoung O. Ahn , Jong W. Kwon , Won B. Kim , Myung G. Lee

Konu :

Pharmacology (medical), Pharmacology, Pharmaceutical Science, General Medicine

Yıl :

2003

Ji M. Jang