The Siamese crocodile (Crocodylus siamensis), listed in CITES Appendix I, is considered one of the most critically endangered crocodilians in the world, and the reintroductions of it have been tried. Investigation of genetic variation for Siamese crocodile can help to conserve and improve this endangered species. Fourteen microsatellite loci were developed and twelve polymorphic loci were used to investigate the genetic variation and genetic bottleneck hypothesis on 48 captive individuals sampled in Guangdong Provincial Wildlife Rescue Center in Guangzhou. The allele number of polymorphic markers ranged from 2 to 10 per locus, with the average of 4.357. Observed heterozygosity ranged from 0.063 to 0.649 and expected values ranged from 0.259 to 0.844. The Shannon information index and Polymorphic Information Content showed that most of the loci were highly informative with an overall mean of 0.941 and 0.440, respectively. The bottleneck analysis provided evidence of a significant genetic signature of population decline.
The outbreak of a novel corona Virus Disease 2019 (COVID-19) in the city of Wuhan, China has resulted in more than 1.7 million laboratory confirmed cases all over the world. Recent studies showed that SARS-CoV-2 was likely originated from bats, but its intermediate hosts are still largely unknown. In this study, we assembled the complete genome of a coronavirus identified in 3 sick Malayan pangolins. The molecular and phylogenetic analyses showed that this pangolin coronavirus (pangolin-CoV-2020) is genetically related to the SARS-CoV-2 as well as a group of bat coronaviruses but do not support the SARS-CoV-2 emerged directly from the pangolin-CoV-2020. Our study suggests that pangolins are natural hosts of Betacoronaviruses. Large surveillance of coronaviruses in pangolins could improve our understanding of the spectrum of coronaviruses in pangolins. In addition to conservation of wildlife, minimizing the exposures of humans to wildlife will be important to reduce the spillover risks of coronaviruses from wild animals to humans.
DOI : 10.1371/journal.ppat.1008421
Cilt: 16 Sayı: 5 Sayfa: e1008421
Wild-caught snakes are a popular and traditional food in China. However, little known to the public, snakes are also intermediate hosts of Spirometra erinaceieuropaei, a food- and water-borne pathogen of sparganosis. Therefore, we investigated the prevalence of S. erinaceieuropaei in 10 popular species of wild-caught snakes in Guangzhou City (Guangdong Province) between July 2009 and July 2010. One hundred and twenty-four specimens of 10 species (including Enhydris plumbea, Zoacys dhumnades, Elaphe radiate, Elaphe taeniura, Elaphe carinata, Ptyas mucosus, Ptyas korros, Naja naja atra, Bungarus fasciatus, and Bungarus multicinctus) were randomly selected from a total of 1,160 wild-caught snakes. They were obtained from food markets in 5 representative districts (Huadou, Panyu, Tianhe, Haizhu, and Conghua). The specimens were killed, necropsied, and examined for parasitic helminths. Of the snakes examined, 29.8% were infected by spargana and the worm burden per infected snake ranged from 1 to 221. Most species were infected except for En. plumbea, B. fasciatus, and B. multicinctus. Prevalence even reached 100% in Zoacys dhumnades. More than half (53.5%) of the spargana were located in muscular tissue, 36.4% in subcutaneous tissue, and 10.1% in the coelomic cavity. The study revealed the potential risk for the zoonotic sparganosis by eating wild-caught snakes and will be helpful in arousing public health concern about the consumption of snake meat.
Cilt: 97 Sayı: 1 Sayfa: pp. 170-171 Yayın Tarihi: 2011-02-01 ISSN: 0022-3395