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SARS-CoV-2: characteristics and current advances in research
Dergi Makalesi

Dergi Makalesi
Open Access
Kaynak :
Directory of Open Access Journals (DOAJ)
Dergi:
Virology Journal
Yazar(lar) :
Yicheng Yang , Zhiqiang Xiao , Kaiyan Ye , Xiaoen He , Bo Sun , Zhiran Qin , Jianghai Yu , Jinxiu Yao , Qinghua Wu , Zhang Bao , Wei Zhao
Yıl :
2020
Jinxiu Yao
Yayıncı Web Sitesi
 Piri Tam Metin Bulucu
Kaydet Alıntıla
Abstract Coronavirus disease 2019 (COVID-19) caused by SARS-CoV-2 infection has spread rapidly across the world and become an international public health emergency. Both SARS-CoV-2 and SARS-CoV belong to subfamily Coronavirinae in the family Coronaviridae of the order Nidovirales and they are classified as the SARS-like species while belong to different cluster. Besides, viral structure, epidemiology characteristics and pathological characteristics are also different. We present a comprehensive survey of the latest coronavirus—SARS-CoV-2—from investigating its origin and evolution alongside SARS-CoV. Meanwhile, pathogenesis, cardiovascular disease in COVID-19 patients, myocardial injury and venous thromboembolism induced by SARS-CoV-2 as well as the treatment methods are summarized in this review.
DOI : 10.1186/s12985-020-01369-z
Anahtar Kelimeler : SARS-CoV-2, COVID-19, Cytokine storm, Cardiovascular disease, Myocardial injury, Venous thromboembolism
ISSN: 1743-422X
Cilt: 17
Sayı: 1
Sayfa: 1 - 17
Sınıflandırma :
2
A signal amplification system on a lateral flow immunoassay detecting for hepatitis e‐antigen in human blood samples
Dergi Makalesi

Dergi Makalesi
Kaynak :
Wiley Online Library
Dergi:
Journal of Medical Virology
Yazar(lar) :
Jinhong Si , Jinfeng Li , Ling Zhang , Weiyun Zhang , Jinxiu Yao , Tingting Li , Wenjing Wang , Weihang Zhu , Jeanu2010Pierre Allain , Yongshui Fu , Chengyao Li
Konu :
Virology, Infectious Diseases
Yıl :
2019
Jinxiu Yao
Yayıncı Web Sitesi
Tam Metin
Kaydet Alıntıla
Hepatitis B e‐antigen (HBeAg) is the secretory form of the nucleocapsid of the hepatitis B virus (HBV), which is a marker of viral replication. In this study, a novel signal amplification system (SAS) based on the lateral flow immunoassay (LFIA) was used for rapid detection of HBeAg in blood samples from patients or blood donors. In this assay, the detection antibody was conjugated with gold nanoparticles (GNPs), and the capture antibody was labeled with biotin. The presence of targeting antigen HBeAg in blood sample would act as a bridge with biotinylated captured antibody and GNP‐conjugated detection antibody to form the dendritic nanoparticle complex. The dendritic complexes in the sample solution were migrated and immobilized on the testing line of strip coated with antibiotin antibodies. Signal intensity was massively amplified by the SAS, which was positively correlated with the concentration of targeting antigen in the blood sample and was assessed by eyes or strip scanner. The SAS worked only when targeting antigens were present in the sample. By using this SAS‐LFIA, we were able to detect a very low concentration of HBeAg (9 ng/mL), which was 27‐fold sensitive than that by conventional LFIA (cLFIA). A number of 420 blood samples were detested by this novel SAS‐LFIA, the results were in accordance with those of enzyme‐linked immunosorbent assay (ELISA) completely, while the cLFIA missed an HBeAg‐positive sample. In conclusion, the novel SAS has high specificity and sensitivity, which can be used to replace the conventional rapid test and ELISA in clinical diagnosis.
DOI : 10.1002/jmv.25452
ISSN: 0146-6615
Sayı: 7
Cilt: 91
Sayfa: 1301-1306
3
High-specificity targets in SARS-CoV-2 N protein for serological detection and distinction from SARS-CoV
Dergi Makalesi

Dergi Makalesi
Kaynak :
Science Direct
Dergi:
Computers in Biology and Medicine
Yayıncı:
Elsevier BV
Yazar(lar) :
Jianhai Yu , Zhiran Qin , Xuling Liu , Xiaoen He , Jinxiu Yao , Xuan Zhou , Kun Wen , Nan Yu , Qinghua Wu , Weiwei Xiao , Li Zhu , Chengsong Wan , Bao Zhang , Wei Zhao
Yıl :
2022
Jinxiu Yao
Yayıncı Web Sitesi
Tam Metin
Kaydet Alıntıla
Numerous serological detection kits are being rapidly developed and approved for screening and diagnosing suspected coronavirus disease 2019 (COVID-19) cases. However, cross-reactivity between pre-existing antibodies against other coronaviruses and the captured antigens in these kits can affect detection accuracy, emphasizing the necessity for identifying highly specific antigen fragments for antibody detection. Thus, we performed a conservation and specificity analysis of the novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) nucleocapsid (N) protein. We also integrated various B-cell epitope prediction methods to obtain possible dominant epitope regions for the N protein, analyzed the differences in serological antibody levels for different epitopes using ELISA, and identified N protein epitopes for IgG and IgM with high-specificity. The SARS-CoV-2 N protein showed low mutation rates and shared the highest amino acid similarity with SARS-CoV; however, it differed substantially from other coronaviruses. Tests targeting the SARS-CoV-2 N protein produce strong positive results in patients recovering from SARS-CoV. The N18–39 and N183-197 epitopes for IgG and IgM detection, respectively, can effectively overcome cross-reactivity, and even exhibit good specificity between SARS-CoV-2 and SARS-CoV. The antibody levels detected with these were consistent with those detected using the complete N protein. These findings provide a basis for serological diagnosis and determining the kinetics of SARS-CoV-2 antibody detection in patients.
DOI : 10.1016/j.compbiomed.2022.105272
Anahtar Kelimeler : Novel coronavirus (SARS-CoV-2), Serological detection, N protein, Cross-reactivity, Epitope
ISSN: 0010-4825
Cilt: 143
Sayfa: 105272
4
Photodynamic inactivation of Shigella flexneri by curcumin
Dergi Makalesi

Dergi Makalesi
Kaynak :
Science Direct
Yazar(lar) :
Zuxin Liang , Xuling Liu , Zhiran Qin , Jingshu Li , Jianhai Yu , Xiaoen He , Li Zhu , Hongying Fan , Yushan Hu , Jinxiu Yao , Chenguang Shen , Chengsong Wan , Bao Zhang , Wei Zhao
Yıl :
2022
Jinxiu Yao
Yayıncı Web Sitesi
Tam Metin
Kaydet Alıntıla
This study aimed to inactivate Shigella flexneri using curcumin-based photosensitization and determine the sensitivity of S. flexneri to the combination of epsilon (ε)-polylysine (ε-PL) and curcumin-based photosensitization for decontamination. Bacterial numbers were reduced by 4.65 log CFU/mL after incubation with 80 μM curcumin and illumination for 20 min. Photosensitization resulted in cell morphology changes and increased cell permeability and protein degradation. Oxidative stress gene (oxyR) and efflux pump genes (marA, acrA) were upregulated. Decontamination of S. flexneri after incubation with curcumin and ε-PL for 1 h followed by photoactivation for 60 s has been investigated. It was found that the growth of bacteria was completely inhibited. Additionally, the number of bacteria was reduced by > 6 log CFU/mL. Experiments on the uptake of curcumin molecules by bacteria showed that ε-PL promoted the penetration of bacterial cells by curcumin, thus enhancing the effects of photosensitization.
DOI : 10.1016/j.lwt.2021.112491
Anahtar Kelimeler : Photosensitization, Curcumin, Shigella flexneri, Epsilon (ε)-polylysine
ISSN: 0023-6438
Cilt: 153
Sayfa: 112491

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