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Mesenchymal potentials of the trunk neural crest cells
Tez

Tez
Open Access
Kaynak :
Dissertation.com
Dergi:
Université Paris-Sud – Paris XI
Yayıncı:
Université Paris-Sud – Paris XI
Yazar(lar) :
Juliana De Mattos Coelho Aguiar
Konu :
Neural crest ; Osteoblasts ; Stem cells , Adipocytes ; Différentiation ; Culture in vitro
Yıl :
2012
Juliana De Mattos Coelho Aguiar
Tam Metin
Kaydet Alıntıla
La crête neurale (CN) dérive de la partie dorsale du tube neural des Vertébrés. Pendant le développement, ces cellules migrent et contribuent à la formation de différents tissus et organes. Le long de l'axe antéro-postérieur, la CN donne naissance aux neurones et cellules gliales du système nerveux périphérique, et aux mélanocytes. Par ailleurs, la CN céphalique est aussi à l’origine de tissus mésenchymateux qui constituent tous les os et cartilages de la face, la plus grande partie du crâne, le derme facial, et les adipocytes et cellules de muscles lisses dans la tête. Dans le tronc des Vertébrés amniotes, ces tissus dérivent du mésoderme. Chez les Vertébrés inférieurs, la CN troncale génère cependant des tissus mésenchymateux, comme les rayons des nageoires du poisson-zèbre. La question qui se pose est de savoir si la capacité de la CN à produire des cellules mésenchymateuses a été totalement perdue dans le tronc au cours de l’évolution, ou bien si elle peut encore se manifester chez les Amniotes dans des conditions spécifiques. Ce travail s’est intéressé à dévoiler le potentiel mésenchymateux de la CN troncale.Notre approche expérimentale a été d'examiner le potentiel de différenciation squelettogénique et adipogénique des cellules de la CN troncale de caille en culture in vitro, par hybridation in situ (HIS), immunocytochimie et RT-PCR. L’ostéogenèse a été initialement caractérisée par l'expression de Runx2, premier facteur de transcription des ostéoprogéniteurs, qui a été détectée par HIS à partir 5 jours de culture. Plus tard, nous avons observé la maturation des ostéoblastes, avec l’expression de la protéine collagen1, des ARNm de l'ostéopontine et de la phosphatase alcaline, jusqu’à l'étape de minéralisation de la matrice osseuse. Les cellules de CN troncale ont effectué également un processus de chondrogenèse, mis en évidence par l'expression des ARNm de Sox9, aggrecan et collagène10, et par la coloration au bleu Alcian. L'observation des zones minéralisées et des régions chondrogéniques suggère que les cellules de la CN troncale in vitro effectuent une ossification de types endochondral et intramembranaire. Dans les mêmes conditions de culture, les cellules se sont aussi différenciées en adipocytes, identifiés à partir de 10 jours de culture par le colorant Oil Red O. Les ARNm des facteurs de transcription CEBP et PPAR, essentiels pour l'adipogenèse, et de la protéine FABP4, ont été détectés par RT-PCR dès 3 jours de culture. Afin de caractériser les précurseurs des cellules osseuses et adipocytaires, nous avons examiné le potentiel de différenciation des cellules individuelles de la CN troncale. L'analyse des types cellulaires dans les cultures clonales a montré que 76% des clones contiennent des ostéoblastes Runx2-positifs. De plus, les cellules de CN troncale comprennent des progéniteurs multipotents dotés à la fois de potentiels neuraux et ostéogénique. Dans une autre condition de culture clonale, les adipocytes ont été trouvés dans la descendance de 35,3% des cellules, et environ la moitié de ces…
Tez türü: phdthesis
2
Laminin and Environmental Cues Act in the Inhibition of the Neuronal Differentiation of Enteric Glia in vitro
Dergi Makalesi

Dergi Makalesi
Open Access
Kaynak :
Directory of Open Access Journals (DOAJ)
Dergi:
Frontiers in Neuroscience
Yazar(lar) :
Carla Pires Veríssimo , Carla Pires Veríssimo , Carla Pires Veríssimo , Juliana da Silva Carvalho , Fábio Jorge Moreira da Silva , Loraine Campanati , Vivaldo Moura-Neto , Vivaldo Moura-Neto , Juliana de Mattos Coelho-Aguiar , Juliana de Mattos Coelho-Aguiar
Yıl :
2019
Juliana de Mattos Coelho-Aguiar Juliana de Mattos Coelho-Aguiar
Yayıncı Web Sitesi
 Piri Tam Metin Bulucu
Kaydet Alıntıla
The enteric glia, a neural crest-derived cell type that composes the Enteric Nervous System, is involved in controlling gut functions, including motility, gut permeability, and neuronal communication. Moreover this glial cell could to give rise to new neurons. It is believed that enteric neurons are generated up to 21 days postnatally; however, adult gut cells with glial characteristics can give rise to new enteric neurons under certain conditions. The factors that activate this capability of enteric glia to differentiate into neurons remain unknown. Here, we followed the progress of this neuronal differentiation and investigated this ability by challenging enteric glial cells with different culture conditions. We found that, in vitro, enteric glial cells from the gut of adult and neonate mice have a high capability to acquire neuronal markers and undergoing morphological changes. In a co-culture system with 3T3 fibroblasts, the number of glial cells expressing βIIItubulin decreased after 7 days. The effect of 3T3-conditioned medium on adult cells was not significant, and fewer enteric glial cells from neonate mice began the neurogenic process in this medium. Laminin, an extracellular matrix protein that is highly expressed by the niche of the enteric ganglia, seemed to have a large role in inhibiting the differentiation of enteric glia, at least in cells from the adult gut. Our results suggest that, in an in vitro approach that provides conditions more similar to those of enteric glial cells in vivo, these cells could, to some extent, retain their morphology and marker expression, with their neurogenic potential inhibited. Importantly, laminin seemed to inhibit differentiation of adult enteric glial cells. It is possible that the differentiation of enteric glia into neurons is related to severe changes in the microenvironment, leading to disruption of the basement membrane. In summary, our data indicated that the interaction between the enteric glial cells and their microenvironment molecules significantly affects the control of their behavior and functions.
DOI : 10.3389/fnins.2019.00914
Anahtar Kelimeler : enteric glia, neurogenesis, laminin, cell culture, microenvironment
Cilt: 13
Sınıflandırma :
3
The Enteric Glia: Identity and Functions
Dergi Makalesi

Dergi Makalesi
Kaynak :
Wiley Online Library
Yazar(lar) :
Juliana de Mattos Coelho-Aguiar , Ana Carina Bon-Frauches , Ana Lúcia Tavares Gomes , Carla Pires Veríssimo , Diego Pinheiro Aguiar , Diana Matias , Beatriz Bastos de Moraes Thomasi , Antoniella Souza Gomes , Gerly Anne de Castro Brito , Vivaldo Moura-Neto
Yıl :
2015
Juliana de Mattos Coelho-Aguiar
Yayıncı Web Sitesi
Tam Metin
Kaydet Alıntıla
Enteric glial cells were first described at the end of the 19th century, but they attracted more interest from researchers only in the last decades of the 20th. Although, they have a different embryological origin, the enteric GLIA share many characteristics with astrocytes, the main glial cell type of the central nervous system (CNS), such as in their expression of the same markers and in their functions. Here we review the construction of the enteric nervous system (ENS), with a focus on enteric glia, and also the main studies that have revealed the action of enteric glia in different aspects of gastrointestinal tract homeostasis, such as in the intestinal barrier, in communications with neurons, and in their action as progenitor cells. We also discuss recent discoveries about the roles of enteric glia in different disorders that affect the ENS, such as degenerative pathologies including Parkinson's and prion diseases, and in cases of intestinal diseases and injury. GLIA 2015;63:921–935
DOI : 10.1002/glia.22795
ISSN: 0894-1491
Sayı: 6
Cilt: 63
Sayfa: 921-935
4
New strategy to control cell migration and metastasis regulated by CCN2/CTGF.
e-Kitap

e-Kitap
Open Access
Kaynak :
Internet Archive Books
Dergi:
Cancer Cell International
Yayıncı:
Cancer Cell International
Yazar(lar) :
Jose Garcia Ribeiro Abreu , Maria Eugenia Leite Duarte , Priscila Ladeira Casado , Julie Calixto Lobo , Juliana de Mattos Coelho-Aguiar , Eduardo Branco de Sousa , Gabriel Correa de Farias , Diego Pinheiro Aguiar
Yıl :
2014
Juliana de Mattos Coelho-Aguiar
Yayıncı Web Sitesi
Tam Metin
Kaydet Alıntıla
This article is from Cancer Cell International, volume 14. Abstract Connective tissue growth factor (CTGF)/CCN family member 2 (CCN2) is a CCN family member of matricellular signaling modulators. It has been shown that CCN2/CTGF mediates cell adhesion, aggregation and migration in a large variety of cell types, including vascular endothelial cells, fibroblasts, epithelial cells, aortic smooth muscle and also pluripotent stem cells. Others matricellular proteins are capable of interacting with CCN2/CTGF to mediate its function. Cell migration is a key feature for tumor cell invasion and metastasis. CCN2/CTGF seems to be a prognostic marker for cancer. In addition, here we intend to discuss recent discoveries and a new strategy to develop therapies against CCN2/CTGF, in order to treat cancer metastasis.
DOI : doi:10.1186/1475-2867-14-61
Cilt :14
ISSN :1475-2867
Sınıflandırma :
5
The Enteric Glial Network Acts in the Maintenance of Intestinal Homeostasis and in Intestinal Disorders
e-Kitap

e-Kitap
Open Access
Kaynak :
IntechOpen
Yazar(lar) :
Juliana de Mattos Coelho-Aguiar , Carla Pires Veríssimo , Deiziane Viana da Silva Costa , Beatriz Bastos de Moraes Thomasi , Ana Carina Bon Frauches , Fabiana Pereira Ribeiro , Ana Lucia Tavares Gomes , Gerly Anne de Castro Brito , Vivaldo Moura-Neto
Yıl :
2019
Juliana de Mattos Coelho-Aguiar
Yayıncı Web Sitesi
Tam Metin
Kaydet Alıntıla
The enteric nervous system (ENS), also known as second brain, innervates our gastrointestinal tract controlling its functions, such as motility, fluid secretion, nutrient absorption, and even involvement in the control of immunity and inflammatory processes. In the gut, the gliocytes are known as enteric glial cells (EGCs). Enteric glial cells form a network that permeates the entire gut. Enteric glia express the cell surface hemichannel of connexin-43 (Cx43) necessary for the propagation of Ca2 + responses, necessary to maintain their functions. In this chapter, besides the development of ENS and its glial cells and the similarities with the astrocytes in the central nervous system, we approached the important role of the glial network in the control of gut homeostasis, in the interaction with the immune system, and its participation in pathological conditions. EGCs are even capable of replacing lost neurons. Thus the enteric glia is a multifunctional cell, which through its multiple interactions maintains the integrity of the ENS allowing it to be resistant to the different and constant aggressions suffered by the digestive system.
DOI : 10.5772/intechopen.89170
Anahtar Kelimeler : enteric neurodegeneration, gut inflammation, gut homeostasis, glial network, enteric glial cells
Sınıflandırma :
Biyotıp %100
Nörokimya %59
6
Neuromechanisms of SARS-CoV-2: A Review
Dergi Makalesi

Dergi Makalesi
Open Access
Kaynak :
Directory of Open Access Journals (DOAJ)
Dergi:
Frontiers in Neuroanatomy
Yazar(lar) :
Marcos F. DosSantos , Marcos F. DosSantos , Marcos F. DosSantos , Sylvie Devalle , Veronica Aran , Daniela Capra , Daniela Capra , Daniela Capra , Natália Roberta Roque , Juliana de Mattos Coelho-Aguiar , Tânia Cristina Leite de Sampaio e Spohr , Tânia Cristina Leite de Sampaio e Spohr , Janice Gonçalves Subilhaga , Cláudia Maria Pereira , Isabella D'Andrea Meira , Isabella D'Andrea Meira , Isabella D'Andrea Meira , Paulo Niemeyer Soares Filho , Vivaldo Moura-Neto , Vivaldo Moura-Neto , Vivaldo Moura-Neto , Vivaldo Moura-Neto , Vivaldo Moura-Neto
Yıl :
2020
Juliana de Mattos Coelho-Aguiar
Yayıncı Web Sitesi
 Piri Tam Metin Bulucu
Kaydet Alıntıla
Recent studies have suggested the neuroinvasive potential of severe acute respiratory coronavirus 2 (SARS-CoV-2). Notably, neuroinvasiveness might be involved in the pathophysiology of coronavirus disease 2019 (COVID-19). Some studies have demonstrated that synapse-connected routes may enable coronaviruses to access the central nervous system (CNS). However, evidence related to the presence of SARS-CoV-2 in the CNS, its direct impact on the CNS, and the contribution to symptoms suffered, remain sparse. Here, we review the current literature that indicates that SARS-CoV-2 can invade the nervous system. We also describe the neural circuits that are potentially affected by the virus and their possible role in the progress of COVID-19. In addition, we propose several strategies to understand, diagnose, and treat the neurological symptoms of COVID-19.
DOI : 10.3389/fnana.2020.00037
Anahtar Kelimeler : SARS-CoV-2, COVID-19, central nervous system, peripheral nervous system, anosmia, dysgeusia
ISSN: 1662-5129
Cilt: 14
Sınıflandırma :

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