Piri Keşif Aracı

Ek Bilgiler

Kaydet

Kaynak 1:

http://dx.doi.org/10.1016/j.nbt.2012.08.439 arayıcılığı görüntüle.
Kaynak 2:

https://aperta.ulakbim.gov.tr/record/104676 arayıcılığı görüntüle.

DOI : 10.1016/j.nbt.2012.08.439

ISSN: 1871-6784
Cilt: 29
Sayfa: S158

Sınıflandırma :

Tıp ve Halk Sağlığı %100

Damar Ameliyatı %96

2

In vitro and in vivo evaluation of the toxicological and molecular effects of a novel Pd(II) complex

Dergi Makalesi

Kaynak 1:

Science Direct

Kaynak 2:

Aperta - TÜBİTAK Açık Arşivi

Dergi:

Toxicology Letters

Yayıncı:

Elsevier

Yazar(lar) :

Zelal Adiguzel , Tarik Baykal , Yuksel Cetin , Omer Kacar , Muge Serhatli , Hivda Polat , Engin Ulukaya , Veysel T. Yilmaz , Ceyda Acilan

Yıl :

2013

Muge Serhatli

Ek Bilgiler

Kaydet

Kaynak 1:

http://dx.doi.org/10.1016/j.toxlet.2013.05.646 arayıcılığı görüntüle.
Kaynak 2:

https://aperta.ulakbim.gov.tr/record/91512 arayıcılığı görüntüle.

DOI : 10.1016/j.toxlet.2013.05.646

ISSN: 0378-4274
Cilt: 221
Sayfa: S73-S74

Sınıflandırma :

Biyotıp %99

Nörokimya %81

3

Evaluation of apoptotic molecular pathways for smooth muscle cells isolated from thoracic aortic aneurysms in response to oxidized sterols

Dergi Makalesi

Kaynak 1:

Springer Link

Kaynak 2:

Aperta - TÜBİTAK Açık Arşivi

Dergi:

Molecular Biology Reports

Yazar(lar) :

Zelal Adiguzel , Nazli Arda , Omer Kacar , Muge Serhatli , Serpil Gezer Tas , Ahmet Tarik Baykal , Kemal Baysal , Ceyda Acilan

Konu :

Genetics, Molecular Biology, General Medicine

Yıl :

2014

Muge Serhatli

Özet Ek Bilgiler

Kaydet

Oxysterols, oxygenated derivatives of cholesterol, are found abundantly in the plasma and atherosclerotic plaques, a common risk factor for thoracic aortic aneurysms (TAAs). Among the oxysterols, namely 7-ketocholesterol (7-KC) and 25-hydroxycholesterol (25-OHC), lead both to induction of reactive oxygen species (ROS) in cells and to apoptosis in smooth muscle cells (SMCs) probably due to increased oxidative stress. Since loss of SMCs through apoptosis is a major event in TAA formation, it is important to understand the molecular pathways of apoptosis in response to ROS in TAAs. Very little is known about the effect of oxysterols on TAA SMCs. Therefore, we investigated molecular pathways participating in the oxysterol induced cell death of TAAs. Our results showed that TAA SMCs died mainly as a result of apoptosis as suggested by cellular shrinkage, blebbing, DNA condensation/fragmentation in response to oxysterol treatment. There was no significant difference in oxysterol induced cell death between TAA and control SMCs. Addition of antioxidant molecules prevented cell death, hence ROS appears to be involved in the apoptosis of these cells. While oxysterol treatment increased caspase 3 activity, cell death was not rescued in its absence. Efficient silencing of other targets including apoptotic proteins (p53, Bax), and survival proteins (Akt1, Akt2) showed that apoptosis can occur through p53, and Bax independent pathways. Silencing Akt1 or Akt2 did not lead to further cell death. These results indicate that oxysterols can induce several cell death pathways in TAA SMCs.

Kaynak 1:

http://dx.doi.org/10.1007/s11033-014-3681-9 arayıcılığı görüntüle.
Kaynak 2:

https://aperta.ulakbim.gov.tr/record/99570 arayıcılığı görüntüle.

DOI : 10.1007/s11033-014-3681-9

Anahtar Kelimeler : Thoracic aortic aneurysm, Oxysterols, Oxidative stress, Reactive oxygen species, Apoptosis

ISSN: 0301-4851 1573-4978
Sayı: 12
Cilt: 41
Sayfa: 7875-7884

Sınıflandırma :

Biyotıp %100

Kardiyovasküler Biyoloji %92

4

Preparation and in vitro characterization of vascular endothelial growth factor (VEGF)-loaded poly(D,L-lactic-co-glycolic acid) microspheres using a double emulsion/solvent evaporation technique

Araştırma Makalesi

Kaynak :

Aperta - TÜBİTAK Açık Arşivi

Dergi:

JOURNAL OF MICROENCAPSULATION

Yazar(lar) :

Bahattin M Baysal , Kemal Baysal , Muge Serhatli , Oksan Karal-Yilmaz

Yıl :

2011

Muge Serhatli

Piri Tam Metin Bulucu Özet Ek Bilgiler

Kaydet

Biodegradable Poly(lactic-co-glycolic acid; PLGA), microspheres encapsulating the angiogenic protein recombinant human vascular endothelial growth factor (rhVEGF) were formed to achieve VEGF release in a sustained manner. These microspheres are a promising delivery system which can be used for therapeutic angiogenesis. The PLGA microspheres incorporating two different initial loading amounts of rhVEGF have been prepared by a modified water-in-oil-in-water (w/o/w) double emulsion/solvent evaporation technique. The microspheres have been characterized by particle size distribution, environmental scanning electron microscopy (ESEM), light microscopy, encapsulation efficiency and their degradation was studied in vitro. The rhVEGF released from microspheres was quantified by the competitive enzyme-linked immunosorbent assay (ELISA) and human umbilical vein endothelial cell (HUVEC) proliferation assay was used to assess biological activity of the released VEGF. The microspheres were spherical with diameters of 10-60 mu m and the encapsulation efficiency was between 46% and 60%. The release kinetics of rhVEGF was studied for two different amounts: 5 mu g VEGF (V5) and 50 mu g VEGF (V50) per 500 mg starting polymer. The total protein (VEGF:BSA) release increased up to 4 weeks for two rhVEGF concentrations. The ELISA results showed that the burst release for V5 and V50 microspheres were 4 and 27 ng/mL, respectively. For V5, the microspheres showed an initial burst release, followed by a higher steady-state release until 14 days. VEGF release increased up to 2 weeks for V50 microsphere. HUVEC proliferation assay showed that endothelial cells responded to bioactive VEGF by proliferating and migrating.

DOI : 10.3109/02652048.2010.523795

Sayi :28
issue :1
Sayfa :46-54

Sınıflandırma :

Biyotıp %98

Farmakoloji ve Toksikoloji %71

Biyotıp %19

5

Proteomic Study of the Microdissected Aortic Media in Human Thoracic Aortic Aneurysms

Araştırma Makalesi

Kaynak :

Aperta - TÜBİTAK Açık Arşivi

Dergi:

JOURNAL OF PROTEOME RESEARCH

Yazar(lar) :

Ahmet Tarik Baykal , Seldag Bekpinar , Eylem Tuncer , Ceyda Acilan , Kemal Baysal , Muge Serhatli

Yıl :

2014

Muge Serhatli

Piri Tam Metin Bulucu Özet Ek Bilgiler

Kaydet

Aortic aneurysm is a complex multifactorial disease, and its molecular mechanism is not understood. In thoracic aortic aneurysm (TAA), the expansion of the aortic wall is lead by extracellular matrix (ECM) degeneration in the medial layer, which leads to weakening of the aortic wall. This dilatation may end in rupture andif untreateddeath. The aortic media is composed of vascular smooth muscle cells (VSMCs) and proteins involved in aortic elasticity and distensibility. Delineating their functional and quantitative decrease is critical in elucidating the disease causing mechanisms as well as the development of new preventive therapies. Laser microdissection (LMD) is an advanced technology that enables the isolation of the desired portion of tissue or cells for proteomics analysis, while preserving their integrity. In our study, the aortic media layers of 36 TAA patients and 8 controls were dissected using LMD technology. The proteins isolated from these tissue samples were subjected to comparative proteomic analysis by nano-LCMS/MS, which enabled the identification of 352 proteins in aortic media. Among these, 41 proteins were differentially expressed in the TAA group with respect to control group, and all were downregulated in the patients. Of these medial proteins, 25 are novel, and their association with TAA is reported for the first time in our study. Subsequent analysis of the data by ingenuity pathway analysis (IPA) shows that the majority of differentially expressed proteins were found to be cytoskeletal-associated proteins and components of the ECM which are critical in maintaining aortic integrity. Our results indicate that the protein expression profile in the aortic media from TAA patients differs significantly from controls. Further analysis of the mechanism points to markers of pathological ECM remodeling, which, in turn, affect VSMC cytosolic structure and architecture. In the future, the detailed investigation of the differentially expressed proteins may provide insight into the elucidation of the pathological processes underlying aneurysms.

DOI : 10.1021/pr5006586

Sayi :13
issue :11
Sayfa :5071-5080

Sınıflandırma :

Biyotıp %97

Hücre Biyolojisi %24

6

CRYOPRESERVATION OF SPLEEN AND LYMPH NODES AS A SOURCE OF MONONUCLEAR CELLS TO BE USED FOR THE DEVELOPMENT OF MONOCLONAL ANTIBODY PRODUCING HYBRIDOMA CELLS

Araştırma Makalesi

Kaynak :

Aperta - TÜBİTAK Açık Arşivi

Dergi:

CRYOLETTERS

Yazar(lar) :

Aynur Basalp , Deniz Durali , Safak Isil Cevik , Muge Serhatli , Ibrahim Hatipoglu , Ibrahim Sogut

Yıl :

2011

Muge Serhatli

Piri Tam Metin Bulucu Özet Ek Bilgiler

Kaydet

In the present study, spleen and lymph nodes of mice were cryopreserved as a whole tissue and after thawing, membrane integrity of mononuclear cells was determined by trypan blue exclusion and PI staining. T and B lymphocytes, macrophages and dendritic cells have been isolated from both cryopreserved tissue and analyzed by Flow cytometry. BALB/c mice were immunized with Hepatitis e antigen (HBeAg) and spleen and lymph nodes of mice were cryopreserved for 3 to 10 months. The cells obtained from both tissue were applied to hybridoma technology to understand if the cells keep their viability and functionality. The cells were isolated and fused with F0 mouse myeloma cells and several antibody producing hybrid cells were developed. Results have shown that cryopreserved spleen and lymph nodes of mice can be efficiently used in hybridoma technology for the successful generation of monoclonal antibody producing hybrid cells.

DOI : 10.81043/aperta.89559

Sayi :32
issue :3
Sayfa :266-274

Sınıflandırma :

Biyotıp %100

Antikorlar %88

7

Smooth Muscle Cells Isolated from Thoracic Aortic Aneurysms Exhibit Increased Genomic Damage, but Similar Tendency for Apoptosis

Araştırma Makalesi

Kaynak :

Aperta - TÜBİTAK Açık Arşivi

Dergi:

DNA AND CELL BIOLOGY

Yazar(lar) :

Kemal Baysal , Mutlu Hayran , Altug Tuncer , Zelal Adiguzel , Omer Kacar , Muge Serhatli , Ceyda Acilan

Yıl :

2012

Muge Serhatli

Piri Tam Metin Bulucu Özet Ek Bilgiler

Kaydet

Aortic aneurysms (AA) are characterized by structural deterioration leading to progressive dilation. During the development of AA, two key structural changes are pronounced, one being degradation of extracellular matrix and the other loss of smooth muscle cells (SMCs) through apoptosis. Reactive oxygen species (ROS) are produced above physiological levels in dilated (aneurismal) part of the aorta compared to the nondilated part and they are known to be associated with both the extracellular matrix degradation and the loss of SMCs. In this study, we hypothesized that aneurismal SMCs are more prone to apoptosis and that at least some cells undergo apoptosis due to elevated ROS in the aortic wall. To test this hypothesis, we first isolated SMCs from thoracic aneurismal tissue and compared their apoptotic tendency with normal SMCs in response to H2O2, oxidized sterol, or UV treatment. Exposed cells exhibited morphological changes characteristic of apoptosis, such as cell shrinkage, membrane blebbing, chromatin condensation, and DNA fragmentation. Terminal deoxynucleotidyl transferased UTP nick end labeling (TUNEL) further confirmed the fragmentation of nuclear DNA in these cells. Vascular SMCs were analyzed for their micronuclei (MN) and binucleate (BN) frequency as indicators of genomic abnormality. These data were then compared to patient parameters, including age, gender, hypertension, or aortic diameter for existing correlations. While the tendency for apoptosis was not significantly different compared to normal cells, both the %MN and %BN were higher in aneurismal SMCs. The data suggest that there is increased DNA damage in TAA samples, which might play a pivotal role in disease development.

DOI : 10.1089/dna.2012.1644

Sayi :31
issue :10
Sayfa :1523-1534

Sınıflandırma :

Biyotıp %97

Biyotıp %49

8

Identification of ApoA1, HPX and POTEE genes by omic analysis in breast cancer

Araştırma Makalesi

Kaynak :

Aperta - TÜBİTAK Açık Arşivi

Dergi:

ONCOLOGY REPORTS

Yazar(lar) :

Hakan Savli , Muge Serhatli , Zafer Canturk , Deniz Sunnetci , Ahiviet Tarik Baykal , Naci Cine

Yıl :

2014

Muge Serhatli

Piri Tam Metin Bulucu Özet Ek Bilgiler

Kaydet

Breast cancer is the most common cancer among women and accounts for 23% of all female types of cancers. It is well recognized that breast cancer represents a heterogeneous group of tumors, and the molecular events involved in the progression to cancer remain undetermined. Moreover, available prognostic and predictive markers are not sufficient for the accurate determination of the risk for many breast cancer patients. Thus, it is necessary to discover new molecular markers for accurate prediction of clinical outcome and individualized therapy. In the present study, we performed omics-based whole-genome trancriptomic and whole proteomic profiling with network and pathway analyses of breast tumors to identify gene expression patterns related to clinical outcome. A total of 20 samples from tumors and 14 normal appearing breast tissues were analyzed using both gene expression microarrays and LC-MS/MS. We identified 585 downregulated and 413 upregulated genes by gene expression microarrays. Among these genes, HPX, POTEE and ApoAl were the most significant genes correlated with the proteomic profile. Our data revealed that these identified genes are closely related to breast cancer and may be involved in robust detection of disease progression.

DOI : 10.3892/or.2014.3277

Sayi :32
issue :3
Sayfa :1078-1086

Sınıflandırma :

Biyotıp %99

İnsan Genetiği %31

9

Identification of novel neutralizing single-chain antibodies against vascular endothelial growth factor receptor 2

Dergi Makalesi

Kaynak 1:

Wiley Online Library

Kaynak 2:

Aperta - TÜBİTAK Açık Arşivi

Dergi:

Biotechnology and Applied Biochemistry

Yazar(lar) :

Berrin Erdag , B. Koray Balcioglu , Aylin Ozdemir Bahadir , Muge Serhatli , Omer Kacar , Aydin Bahar , Urartu O. S. Seker , Emel Akgun , Abdulkadir Ozkan , Turker Kilic , Candan Tamerler , Kemal Baysal

Konu :

Biotechnology, Process Chemistry and Technology, Molecular Medicine, Applied Microbiology and Biotechnology, Bioengineering, Drug Discovery, Biomedical Engineering, General Medicine

Yıl :

2011

Muge Serhatli

Özet Ek Bilgiler

Kaydet

Human vascular endothelial growth factor (VEGF) and its receptor (VEGFR‐2/kinase domain receptor [KDR]) play a crucial role in angiogenesis, which makes the VEGFR‐2 signaling pathway a major target for therapeutic applications. In this study, a single‐chain antibody phage display library was constructed from spleen cells of mice immunized with recombinant human soluble extracellular VEGFR‐2/KDR consisting of all seven extracellular domains (sKDR D1–7) to obtain antibodies that block VEGF binding to VEGFR‐2. Two specific single‐chain antibodies (KDR1.3 and KDR2.6) that recognized human VEGFR‐2 were selected; diversity analysis of the clones was performed by BstNI fingerprinting and nucleotide sequencing. The single‐chain variable fragments (scFvs) were expressed in soluble form and specificity of interactions between affinity purified scFvs and VEGFR‐2 was confirmed by ELISA. Binding of the recombinant antibodies for VEGFR‐2 receptors was investigated by surface plasmon resonance spectroscopy. In vitro cell culture assays showed that KDR1.3 and KDR2.6 scFvs significantly suppressed the mitogenic response of human umbilical vein endothelial cells to recombinant human VEGF165 in a dose‐dependent manner, and reduced VEGF‐dependent cell proliferation by 60% and 40%, respectively. In vivo analysis of these recombinant antibodies in a rat cornea angiogenesis model revealed that both antibodies suppressed the development of new corneal vessels (p < 0.05). Overall, in vitro and in vivo results disclose strong interactions of KDR1.3 and KDR2.6 scFvs with VEGFR‐2. These findings indicate that KDR1.3 and KDR2.6 scFvs are promising antiangiogenic therapeutic agents.

Kaynak 1:

https://onlinelibrary.wiley.com/doi/10.1002/bab.61 arayıcılığı görüntüle.
Kaynak 2:

https://aperta.ulakbim.gov.tr/record/89530 arayıcılığı görüntüle.

DOI : 10.1002/bab.61

ISSN: 0885-4513
Sayı: 6
Cilt: 58
Sayfa: 412-422

10

A preliminary proteomic evaluation of smooth muscle cells in thoracic aortic aneurysms

Araştırma Makalesi

Kaynak :

Aperta - TÜBİTAK Açık Arşivi

Dergi:

TURKISH JOURNAL OF BIOLOGY

Yazar(lar) :

Ahmet Tarik Baykal , Kemal Baysal , Serpil Tas , Zelal Adiguzel , Omer Kacar , Muge Serhatli , Betul Baykal , Ceyda Acilan Ayhan

Yıl :

2014

Muge Serhatli

Özet Ek Bilgiler

Kaydet

Aortic aneurysm is characterized as localized degeneration of the aorta leading to advanced weakening and widening of the vessel. While the exact mechanisms have yet to be determined, current studies indicate that the degradation of extracellular matrix (ECM) proteins and apoptosis of vascular smooth muscle cells (SMCs) may result in extendibility, dilation, and rupture of the vessel. Within the aortic wall, SMCs are implicated as key components involved in disease development, as numerous molecular changes have been reported to occur. Most current studies involve either investigation of proteins constituting a group or pathway in SMCs, or analyses of the whole aortic tissue. In order to determine which proteins are important in the development of thoracic aortic aneurysms (TAAs), we performed comparative proteomic analyses using cultured SMCs from TAAs versus controls. Label-free nano LC-MS/MS analysis of cell extracts resulted in the identification of 256 proteins, 26 of which were differentially regulated by >= 1.4-fold. Both previously described and new proteins were identified that were involved in oxidative stress, ECM formation, energy metabolism, or the 14-3-3 pathway. Among these, differential expression of SerpinH1, a protease inhibitor for collagenases, was further verified via immunoblotting. Here we have attempted to shed light on the cellular mechanisms of TAAs.

DOI : 10.3906/biy-1306-24

Sayi :38
issue :2
Sayfa :238-252

Sınıflandırma :

Biyotıp %100

Kardiyovasküler Biyoloji %43

Biyomedikal Mühendisliği ve Biyomühendislik %25

11

Proteomic evidence for the plasticity of cultured vascular smooth muscle cells

Araştırma Makalesi

Kaynak :

Aperta - TÜBİTAK Açık Arşivi

Dergi:

TURKISH JOURNAL OF BIOLOGY

Yazar(lar) :

Ceyda Acilan Ayhan , Kemal Baysal , Omer Kacar , Mehmet Altug Tuncer , Zelal Adiguzel , Muge Serhatli , Betul Baykal , Ahmet Tarik Baykal

Yıl :

2013

Muge Serhatli

Özet Ek Bilgiler

Kaydet

Morphological, functional, and gene expression studies have established the phenotypic plasticity of smooth muscle cells (SMCs). These cells have been shown to respond to environmental stimulants such as extracellular matrix and growth factors. Cellular changes can vary between extremes defined as the contractile and synthetic states. Various growth factors have been shown to have profound effects on the phenotype of these cells. In this study, we intended to investigate the effects of growth factor-rich medium on the protein expression of vascular SMCs in culture. Interestingly, transiently changing the type of medium did not result in any apparent morphological differences, yet we hypothesized that some cellular factors might still be altered. In order to understand what kind of intracellular molecular changes should be expected to occur during the medium change, we analyzed global protein expression changes using nano-LC-MS/MS in smooth muscle cell cultures that were isolated and grown in one medium formulation and temporarily switched to the other. Our data indicate that proteins playing a role in energy metabolism (glycolysis), translation, and folding of proteins are affected, along with regulatory molecules and cytoskeletal proteins. The individual proteins and their significance are discussed within the scope of this paper.

DOI : 10.3906/biy-1208-14

Sayi :37
issue :4
Sayfa :414-425

Sınıflandırma :

Biyotıp %98

Hücre Fizyolojisi %23

Hücre Biyolojisi %21

12

A Proteomic Analysis of Mitochondrial Complex III Inhibition in SH-SY5Y Human Neuroblastoma Cell Line

Araştırma Makalesi

Kaynak :

Aperta - TÜBİTAK Açık Arşivi

Dergi:

CURRENT PROTEOMICS

Yazar(lar) :

Ahmet Tarik Baykal , Busra Gurel , Zelal Adiguzel , Emel Akgun , Betul Sahin , Ceyda Acilan , Muge Serhatli , Mete Bora Tuzuner , Cigdem Acioglu

Yıl :

2019

Muge Serhatli

Piri Tam Metin Bulucu Özet Ek Bilgiler

Kaydet

Background and Objective: Antimycin A (AntA) is a potent Electron Transport System (ETS) inhibitor exerting its effect through inhibiting the transfer of the electrons by binding to the quinone reduction site of the cytochrome bcl complex (Complex III), which is known to be impaired in Huntington's Disease (HD). The current studies were undertaken to investigate the effect of complex III inhibition in the SH-SY5Y cell line to delineate the molecular and cellular processes, which may play a role in the pathogenesis of HD.

DOI : 10.2174/1570164615666180713110139

Sayi :16
issue :2
Sayfa :136-147

Sınıflandırma :

Biyotıp %100

Hücre Biyolojisi %38

Nörokimya %35

13

SARS-CoV-2 neutralizing antibody development strategies

Araştırma Makalesi

Kaynak :

Aperta - TÜBİTAK Açık Arşivi

Dergi:

TURKISH JOURNAL OF BIOLOGY

Yazar(lar) :

Aylin Ozdemir Bahadir , Saban Tekin , Hivda Ulbegi Polat , Muge Serhatli , Filiz Kaya , Fatima Yucel , Hasan Umit Ozturk , Melis Denizci Oncu , Bertan Koray Balcioglu

Yıl :

2020

Muge Serhatli

Özet Ek Bilgiler

Kaydet

In December 2019 a novel coronavirus was detected in Wuhan City of Hubei Province-China. Owing to a high rate of transmission from human to human, the new virus called SARS-CoV-2 differed from others by its unexpectedly rapid spread. The World Health Organization (WHO) described the most recent coronavirus epidemic as a global pandemic in March 2020. The virus spread triggered a health crisis (the COVID-19 disease) within three months, with socioeconomic implications. No approved targeted-therapies are available for COVID-19, yet. However, it is foreseen that antibody-based treatments may provide an immediate cure for patients. Current neutralizing antibody development studies primarily target the S protein among the structural elements of SARS-CoV-2, which mediates the cell entry of the virus through the angiotensin converting enzyme 2 (ACE2) receptor of host cells. This review aims to provide some of the neutralizing antibody development strategies for SARS-CoV-2 and in vitro and in vivo neutralization assays.

DOI : 10.3906/biy-2005-91

Sayi :44
issue :3
Sayfa :203-214

Sınıflandırma :

Biyotıp %100

Viroloji %100

14

mTor Is a Signaling Hub in Cell Survival: A Mass-Spectrometry-Based Proteomics Investigation

Araştırma Makalesi

Kaynak :

Aperta - TÜBİTAK Açık Arşivi

Dergi:

JOURNAL OF PROTEOME RESEARCH

Yazar(lar) :

Jin-Jing Pei , Bengt Winblad , Zhizhong Guan , Yan Zhang , Xinying Ji , Eniko Ioja , Shan Wang , Cigdem Acioglu , Betul Baykal , Muge Serhatli , Erika Bereczki , Haiyan Zhang , Hernan Concha Quezada , Hui Gao , Ahmet Tank Baykal , Zhi Tang

Yıl :

2014

Muge Serhatli