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Resveratrol Inhibits Oxidative Stress and Prevents Mitochondrial Damage Induced by Zinc Oxide Nanoparticles in Zebrafish (Danio rerio)
Dergi Makalesi

Dergi Makalesi
Open Access
Kaynak 1:
Directory of Open Access Journals (DOAJ)
Kaynak 2:
MDPI
Dergi:
International Journal of Molecular Sciences
Yazar(lar) :
Roberta Giordo , Gheyath K. Nasrallah , Ola Al-Jamal , Panagiotis Paliogiannis , Gianfranco Pintus
Yıl :
2020
Ola Al-Jamal
Piri Tam Metin Bulucu
Kaydet Alıntıla
Despite their wide industrial use, Zinc oxide (ZnO) nanoparticles (NPs) exhibit a high toxic potential while concerns of their health-related risks are still present, urging additional in vivo clarification studies. Oxidative stress is recognized as the primary trigger of NP-associated toxicity, suggesting antioxidants as a promising counteractive approach. Here, we investigated the protective effect of the natural antioxidant resveratrol against ZnO NP-induced toxicity in vivo using the zebrafish model. Our findings demonstrate that resveratrol counteracts ZnO NP-induced zebrafish lethality preventing cardiac morphological and functional damage. NP-induced vascular structural abnormalities during embryonic fish development were significantly counteracted by resveratrol treatment. Mechanistically, we further showed that resveratrol inhibits ROS increase, prevents mitochondrial membrane potential dysfunction, and counteracts cell apoptosis/necrosis elicited by ZnO NP. Overall, our data provide further evidence demonstrating the primary role of oxidative stress in NP-induced damage, and highlight new insights concerning the protective mechanism of antioxidants against nanomaterial toxicity.
DOI : 10.3390/ijms21113838
Anahtar Kelimeler : resveratrol, nanoparticles, oxidative stress, antioxidants, mitochondria
Cilt: 21
Sayı: 3838
Sayfa: 3838
Sınıflandırma :
Apoptoz %87
2
Challenges in Laboratory Diagnosis of the Novel Coronavirus SARS-CoV-2
Dergi Makalesi

Dergi Makalesi
Open Access
Kaynak 1:
Directory of Open Access Journals (DOAJ)
Kaynak 2:
MDPI
Dergi:
Viruses
Yazar(lar) :
Nadin Younes , Duaa W. Al-Sadeq , Hadeel AL-Jighefee , Salma Younes , Ola Al-Jamal , Hanin I. Daas , Hadi. M. Yassine , Gheyath K. Nasrallah
Yıl :
2020
Ola Al-Jamal
Piri Tam Metin Bulucu
Kaydet Alıntıla
The recent outbreak of the Coronavirus disease 2019 (COVID-19) has quickly spread worldwide since its discovery in Wuhan city, China in December 2019. A comprehensive strategy, including surveillance, diagnostics, research, clinical treatment, and development of vaccines, is urgently needed to win the battle against COVID-19. The past three unprecedented outbreaks of emerging human coronavirus infections at the beginning of the 21st century have highlighted the importance of readily available, accurate, and rapid diagnostic technologies to contain emerging and re-emerging pandemics. Real-time reverse transcriptase-polymerase chain reaction (rRT-PCR) based assays performed on respiratory specimens remain the gold standard for COVID-19 diagnostics. However, point-of-care technologies and serologic immunoassays are rapidly emerging with high sensitivity and specificity as well. Even though excellent techniques are available for the diagnosis of symptomatic patients with COVID-19 in well-equipped laboratories; critical gaps still remain in screening asymptomatic people who are in the incubation phase of the virus, as well as in the accurate determination of live viral shedding during convalescence to inform decisions for ending isolation. This review article aims to discuss the currently available laboratory methods and surveillance technologies available for the detection of COVID-19, their performance characteristics and highlight the gaps in current diagnostic capacity, and finally, propose potential solutions. We also summarize the specifications of the majority of the available commercial kits (PCR, EIA, and POC) for laboratory diagnosis of COVID-19.
DOI : 10.3390/v12060582
Anahtar Kelimeler : COVID-19, SARS-CoV-2, viruses, diagnostic challenges, molecular testing, serology
ISSN: 1999-4915
Cilt: 12
Sayı: 582
Sayfa: 582
Sınıflandırma :
3
Ecotoxicological Assessment of Thermally- and Hydrogen-Reduced Graphene Oxide/TiO2 Photocatalytic Nanocomposites Using the Zebrafish Embryo Model
Dergi Makalesi

Dergi Makalesi
Open Access
Kaynak 1:
MDPI
Kaynak 2:
Directory of Open Access Journals (DOAJ)
Dergi:
Nanomaterials
Yayıncı:
MDPI AG
Yazar(lar) :
Halema Al-Kandari , Nadin Younes , Ola Al-Jamal , Zain Z. Zakaria , Huda Najjar , Farah Alserr , Gianfranco Pintus , Maha A. Al-Asmakh , Aboubakr M. Abdullah , Gheyath K. Nasrallah
Yıl :
2019
Ola Al-Jamal
Tam Metin
Kaydet Alıntıla
Advanced oxidation processes (AOPs) have recently attracted great interest in water pollution management. Using the zebrafish embryo model, we investigated the environmental impacts of two thermally (RGOTi)- and hydrogen (H2RGOTi)-reduced graphene oxide/TiO2 semiconductor photocatalysts recently employed in AOPs. For this purpose, acutoxicity, cardiotoxicity, neurobehavioral toxicity, hematopoietic toxicity, and hatching rate were determinate. For the RGOTi, the no observed effect concentration (NOEC, mortality/teratogenicity score <20%) and the median lethal concentration (LC50) were <400 and 748.6 mg/L, respectively. H2RGOTi showed a NOEC similar to RGOTi. However, no significant mortality was detected at all concentrations used in the acutoxicity assay (up to1000 mg/L), thus indicating a hypothetical LC50 higher than 1000 mg/L. According to the Fish and Wildlife Service Acute Toxicity Rating Scale, RGOTi can be classified as “practically not toxic” and H2RGOTi as “relatively harmless”. However, both nanocomposites should be used with caution at concentration higher than the NOEC (400 mg/L), in particular RGOTi, which significantly (i) caused pericardial and yolk sac edema; (ii) decreased the hatching rate, locomotion, and hematopoietic activities; and (iii) affected the heart rate. Indeed, the aforementioned teratogenic phenotypes were less devastating in H2RGOTi-treated embryos, suggesting that the hydrogen-reduced graphene oxide/TiO2 photocatalysts may be more ecofriendly than the thermally-reduced ones.
DOI : 10.3390/nano9040488
ISSN: 2079-4991
Sayı: 4
Cilt: 9
Sayfa: 488
Sınıflandırma :
4
Organ-specific toxicity evaluation of stearamidopropyl dimethylamine (SAPDMA) surfactant using zebrafish embryos
Dergi Makalesi

Dergi Makalesi
Kaynak :
Science Direct
Dergi:
Science of The Total Environment
Yayıncı:
Elsevier
Yazar(lar) :
Ola Al-Jamal , Hadeel Al-Jighefee , Nadin Younes , Roba Abdin , Maha A. Al-Asmakh , A. Bahgat Radwan , Mostafa H. Sliem , Amin F. Majdalawieh , Gianfranco Pintus , Hadi M. Yassine , Aboubakr M. Abdullah , Sahar I. Da'as , Gheyath K. Nasrallah
Yıl :
2020
Ola Al-Jamal
Yayıncı Web Sitesi
Tam Metin
Kaydet Alıntıla
Surfactants are widely used in the industry of detergents, household products, and cosmetics. SAPDMA is a cationic surfactant that is used mostly in cosmetics, conditioning agents and has recently gained attention as a corrosion inhibitor in the sea pipelines industry. In this regard, literature concerning the ecotoxicological classification of SAPDMA on aquatic animals is lacking. This study aims to evaluate the potential ecotoxicity of SAPDMA using the aquatic zebrafish embryo model. The potential toxic effects of SAPDMA were assessed by different assays. This includes (i) mortality/survival assay to assess the median lethal concentration (LC50); (ii) teratogenicity assay to assess the no observed effect concentration (NOEC); (iii) organ-specific toxicity assays including cardiotoxicity, neurotoxicity (using locomotion assay), hematopoietic toxicity (hemoglobin synthesis using o-dianisidine staining), hepatotoxicity (liver steatosis and yolk retention using Oil Red O (ORO) stain); (iv) cellular cytotoxicity (mitochondrial membrane potential) by measuring the accumulation of JC-1 dye into mitochondria. Exposure of embryos to SAPDMA caused mortality in a dose-dependent manner with a calculated LC50 of 2.3 mg/L. Thus, based on the LC50 value and according to the Fish and Wildlife Service (FWS) Acute Toxicity Rating Scale, SAPDMA is classified as “moderately toxic”. The No Observed Effect Concentration (NOEC) concerning a set of parameters including scoliosis, changes in body length, yolk, and eye sizes was 0.1 mg/L. At the same NOEC concentration (0.1 mg/L), no organ-specific toxicity was detected in fish treated with SAPDMA, except hepatomegaly with no associated liver dysfunctions. However, higher SAPDMA concentrations (0.8 mg/L) have dramatic effects on zebrafish organ development (eye, heart, and liver development). Our data recommend a re-evaluation of the SAPDMA employment in the industry setting and its strictly monitoring by environmental and public health agencies.
DOI : 10.1016/j.scitotenv.2020.140450
Anahtar Kelimeler : Stearamidopropyl Dimethylamine (SAPDMA) surfactant, Zebrafish, Organ-specific toxicity, LC50, NOEC
ISSN: 0048-9697
Cilt: 741
Sayfa: 140450
Sınıflandırma :
Çevre %71
Kirlilik %74

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