Abstract\n An automated colorimetric method for determination of ascorbic acid has been developed. This method utilizes diazotized 4-methoxy-2-nitroanaline and produces a stable color. It is suitable for direct measurement of ascorbic acid in serum and urine.
Abstract\n The stability of 18 chemical constituents of human serum was measured at various times and temperatures, the measurements being made with the Technicon AutoAnalyzer, by routine N-methods (Technicon laboratory method file number). Data are presented illustrating what happens to human serum under defined conditions of collection and storage. Analysis of the data indicates that under certain conditions the concentration or enzymatic activity of certain constituents may be significantly altered.