• Piri Keşif Aracı

Arama ipuçları nelerdir?
1.  sayfa ve
1
Distribution of TT virus genomic groups 1-5 in Brazilian blood donors, HBV carriers, and HIV-1-infected patients
Dergi Makalesi

Dergi Makalesi
Kaynak :
Wiley Online Library
Dergi:
Journal of Medical Virology
Yazar(lar) :
Sylvie Devalle , Christian Niel
Konu :
Virology, Infectious Diseases
Yıl :
2003
Sylvie Devalle
Yayıncı Web Sitesi
Tam Metin
Kaydet Alıntıla
Human isolates of the highly prevalent TT virus (TTV) have been classified into five major genomic groups (1–5). The geographical distribution of the groups throughout the world is not well known. Five different PCR assays were developed in an attempt to amplify specifically TTV DNAs of each genomic group. Serum samples collected from 72 Brazilian adults (24 voluntary blood donors, 24 hepatitis B virus (HBV) carriers, and 24 human immunodeficiency virus type 1 (HIV‐1)‐infected patients) were tested. TTV DNA from at least one genomic group was detected in 11 (46%) blood donors, 13 (54%) HBV carriers, and 24 (100%) HIV‐1 patients. All five genomic groups were detected in the three populations, with the exception of group 2 in blood donors. Some samples, negative with all five specific assays, were positive with the commonly used untranslated region (UTR) PCR system. On the other hand, TTV DNA was detected in some samples by using specific assays but not with the UTR PCR. Mixed infections with 2–5 TTV isolates from different groups were detected in 21% blood donors, 29% HBV carriers, and 71% HIV‐1 patients. Fifteen PCR products (three obtained with each assay) were sequenced. Most sequences showed high (>86%) homology with those of TTV isolates belonging to their presumed groups. However, three sequences had low homology with all TTV sequences available from the DNA databanks. In conclusion, TTV isolates belonging to all five known genomic groups circulate in Brazil, and the results suggest the existence of new and as yet uncharacterised major genomic groups. J. Med. Virol. 72:166–173, 2004. © 2004 Wiley‐Liss, Inc.
DOI : 10.1002/jmv.10564
ISSN: 0146-6615
Sayı: 1
Cilt: 72
Sayfa: 166-173
2
Genomic characterization of a Brazilian TT Virus isolate closely related to SEN virus-F
Dergi Makalesi

Dergi Makalesi
Open Access
Kaynak :
Directory of Open Access Journals (DOAJ)
Dergi:
Memórias do Instituto Oswaldo Cruz.
Yazar(lar) :
Leonardo Diniz-Mendes , Sylvie Devalle , Christian Niel
Yıl :
2004
Sylvie Devalle
Yayıncı Web Sitesi
 Piri Tam Metin Bulucu
Kaydet Alıntıla
SEN virus (SENV) is a circular, single stranded DNA virus that has been first characterized in the serum of a human immunodeficiency virus type 1 (HIV-1)-infected patient. Eight genotypes of SENV (A-H) have been identified and further recognized as variants of TT virus (TTV) in the family Circoviridae. Here we describe the first genomic characterization of a SENV isolate (5-A) from South America. Using 'universal' primers, able to amplify most, if not all, TTV/SENV genotypes, a segment of > 3 kb was amplified by polymerase chain reaction from the serum of an HIV-1 infected patient. The amplicon was cloned and a 3087-nucleotide sequence was determined, that showed a high (85%) homology with the sequence of the Italian isolate SENV-F. Proteins encoded by open reading frames (ORFs) 1 to 4 consisted of 758, 129, 276, and 267 amino acids, respectively. By phylogenetic analysis, isolate 5-A was classified into TTV genotype 19 (phylogenetic group 3), together with SENV-F and TTV isolate SAa-38.
DOI : 10.1590/S0074-02762004000300011
Anahtar Kelimeler : hepatitis, human immunodeficiency virus type 1, nucleotide sequencing, open reading frames 1-4, SEN virus-F, TT virus
Cilt: 99
Sayı: 3
Sayfa: 301 - 306
Sınıflandırma :
Viroloji %100
3
Generation of urine iPS cell lines from patients with Attention Deficit Hyperactivity Disorder (ADHD) using a non-integrative method
Dergi Makalesi

Dergi Makalesi
Open Access
Kaynak :
Directory of Open Access Journals (DOAJ)
Dergi:
Stem Cell Research
Yazar(lar) :
Jaroslaw Sochacki , Sylvie Devalle , Marcelo Reis , Paulo Mattos , Stevens Rehen
Yıl :
2016
Sylvie Devalle
Yayıncı Web Sitesi
 Piri Tam Metin Bulucu
Kaydet Alıntıla
Urine samples were collected from three patients (2 males, 1 female) with clinically diagnosed Attention Deficit Hyperactivity Disorder (ADHD) according to DSM-5 criteria using semi-structured interviews (K-SADS adapted for adults) by a trained psychiatrist. Urine epithelial cell lines were established and expanded for subsequent reprogramming procedure. Induced pluripotent stem cells (iPSCs) were derived using integration-free CytoTune®-iPS 2.0 Sendai Reprogramming Kit, which includes Sendai virus particles of the four Yamanaka factors Oct3/4, Sox2, Klf4 and c-Myc.
DOI : 10.1016/j.scr.2016.05.015
Cilt: 17
Sayı: 1
Sayfa: 102 - 106
Sınıflandırma :
4
Generation of urine iPS cell line from a patient with obsessive-compulsive disorder using a non-integrative method
Dergi Makalesi

Dergi Makalesi
Open Access
Kaynak :
Directory of Open Access Journals (DOAJ)
Dergi:
Stem Cell Research
Yazar(lar) :
Jaroslaw Sochacki , Sylvie Devalle , Marcelo Reis , Leonardo F. Fontenelle , Stevens Rehen
Yıl :
2016
Sylvie Devalle
Yayıncı Web Sitesi
 Piri Tam Metin Bulucu
Kaydet Alıntıla
Urine sample was collected from a 29-year-old male patient with an early onset form of DSM-5 obsessive-compulsive disorder (OCD), comorbid body dysmorphic and excoriation (skin-picking) disorders, and a positive family history for OCD. Urine cell line was established and expanded for the reprogramming procedure. Induced pluripotent stem (iPS) cells were derived using the integration-free CytoTune®-iPS 2.0 Sendai Reprogramming Kit, which includes Sendai virus particles of the four Yamanaka factors Oct3/4, Sox2, Klf4 and c-Myc.
DOI : 10.1016/j.scr.2016.05.018
Cilt: 17
Sayı: 1
Sayfa: 107 - 110
Sınıflandırma :
5
Centrosomal localisation of the cancer/testis (CT) antigens NY-ESO-1 and MAGE-C1 is regulated by proteasome activity in tumour cells.
Dergi Makalesi

Dergi Makalesi
Open Access
Kaynak :
Directory of Open Access Journals (DOAJ)
Dergi:
PLoS ONE
Yazar(lar) :
Anna Pagotto , Otavia L Caballero , Norbert Volkmar , Sylvie Devalle , Andrew J G Simpson , Xin Lu , John C Christianson
Yıl :
2013
Sylvie Devalle
Yayıncı Web Sitesi
Tam Metin
Kaydet Alıntıla
The Cancer/Testis (CT) antigen family of genes are transcriptionally repressed in most human tissues but are atypically re-expressed in many malignant tumour types. Their restricted expression profile makes CT antigens ideal targets for cancer immunotherapy. As little is known about whether CT antigens may be regulated by post-translational processing, we investigated the mechanisms governing degradation of NY-ESO-1 and MAGE-C1 in selected cancer cell lines. Inhibitors of proteasome-mediated degradation induced the partitioning of NY-ESO-1 and MAGE-C1 into a detergent insoluble fraction. Moreover, this treatment also resulted in increased localisation of NY-ESO-1 and MAGE-C1 at the centrosome. Despite their interaction, relocation of either NY-ESO-1 or MAGE-C1 to the centrosome could occur independently of each other. Using a series of truncated fragments, the regions corresponding to NY-ESO-1(91-150) and MAGE-C1(900-1116) were established as important for controlling both stability and localisation of these CT antigens. Our findings demonstrate that the steady state levels of NY-ESO-1 and MAGE-C1 are regulated by proteasomal degradation and that both behave as aggregation-prone proteins upon accumulation. With proteasome inhibitors being increasingly used as front-line treatment in cancer, these data raise issues about CT antigen processing for antigenic presentation and therefore immunogenicity in cancer patients.
DOI : 10.1371/journal.pone.0083212
ISSN: 1932-6203
Cilt: 8
Sayı: 12
Sayfa: e83212
Sınıflandırma :
6
Prevalência e diversidade genética do torque teno vírus em pacientes com lúpus eritematoso sistêmico em serviço de referência no Mato Grosso do Sul Prevalence and genetic diversity of torque teno virus in patients with systemic lupus erythematosus in a reference service in Mato Grosso do Sul
Dergi Makalesi

Dergi Makalesi
Open Access
Kaynak :
Directory of Open Access Journals (DOAJ)
Dergi:
Revista Brasileira de Reumatologia
Yazar(lar) :
Márcio Reis da Costa , Izaias Pereira da Costa , Sylvie Devalle , Ana Rita Coimbra Motta de Castro , Solange Zacalusni Freitas
Yıl :
2012
Sylvie Devalle
Yayıncı Web Sitesi
 Piri Tam Metin Bulucu
Kaydet Alıntıla
Estudos recentes sobre o torque teno vírus (TTV), gênero Anellovirus, permitiram construir a hipótese de que esse vírus pode ser um desencadeante ou tenha algum papel patogênico nas doenças reumáticas autoimunes. OBJETIVOS: Verificar a frequência da infecção pelo TTV em pacientes com lúpus eritematoso sistêmico (LES), e sua diversidade gênica, a existência de correlação entre a infecção pelo TTV e as manifestações clínicas do LES, sua evolução clínica e o perfil sorológico. PACIENTES E MÉTODOS: Foram obtidas 46 amostras de soro de pacientes com LES atendidos no Ambulatório de Reumatologia do Hospital Universitário de Campo Grande (NHU/FAMED/UFMS). Para os controles, utilizaram-se 46 amostras de soro de doadores de sangue. O DNA viral foi extraído das amostras utilizando o QIAamp DNA Blood Mini Kit (QIAGEN, Hilden, Alemanha), e amplificado utilizando a técnica de nested PCR. RESULTADOS: Foi encontrada positividade para o TTV em 17 (37%) dos pacientes lúpicos, e em apenas sete (15,2%) dos controles (teste z, P = 0,03). Não houve correlação entre a infecção pelo TTV, as manifestações clínicas, o perfil sorológico e a evolução clínica dos pacientes avaliados neste estudo. CONCLUSÃO: A presença do TTV nos pacientes com LES necessita ser mais bem compreendida a partir deste estudo inicial Recent studies on the torque teno virus (TTV), genus Anellovirus, have allowed formulating the hypothesis that TTV may trigger autoimmune rheumatic diseases or have some pathogenic role in them. OBJECTIVES: To determine the frequency of TTV infection in patients with systemic lupus erythematosus (SLE), the genetic diversity of TTV, the correlation between TTV infection and SLE clinical manifestations, and SLE clinical course and serological profile. PATIENTS AND METHODS:Serum samples were obtained from 46 SLE patients treated at the University-Affiliated Hospital of Campo Grande (NHU/FAMED/UFMS), Brazil. For controls, serum samples were obtained from 46 healthy volunteer blood donors. Viral DNA was extracted from samples using the QIAamp DNA Blood Mini Kit (QIAGEN, Hilden, Germany) and amplified using nested PCR. RESULTS: Positivity for TTV was found in 17 (37%) of SLE patients and in only seven (15.2%) of the controls (z test, P = 0.03). There was no correlation between TTV infection, SLE clinical manifestations, SLE clinical course, and the serological profile of the patients evaluated. CONCLUSION: Further studies on the presence of TTV in SLE patients are required
DOI : 10.1590/S0482-50042012000100006
Anahtar Kelimeler : Anellovirus, doenças autoimunes, lúpus eritematoso sistêmico, torque teno vírus, Anellovirus, autoimmune diseases, systemic lupus erythematosus, torque teno virus
Cilt: 52
Sayı: 1
Sayfa: 49 - 54
7
Osteosarcoma, chondrosarcoma and Ewing sarcoma: Clinical aspects, biomarker discovery and liquid biopsy
Dergi Makalesi

Dergi Makalesi
Kaynak :
Science Direct
Dergi:
Critical Reviews in Oncology/Hematology
Yayıncı:
Elsevier BV
Yazar(lar) :
Veronica Aran , Sylvie Devalle , Walter Meohas , Manoela Heringer , Anabela Cunha Caruso , Diego Pinheiro Aguiar , Maria Eugênia Leite Duarte , Vivaldo Moura Neto
Yıl :
2021
Sylvie Devalle
Yayıncı Web Sitesi
Tam Metin
Kaydet Alıntıla
Bone sarcomas, although rare, are associated with significant morbidity and mortality. The most frequent primary bone cancers include osteosarcoma, chondrosarcoma and Ewing sarcoma. The treatment approaches are heterogeneous and mainly chosen based on precise tumour staging. Unfortunately, clinical outcome has not changed significantly in over 30 years and tumour grade is still the best prognosticator of metastatic disease and survival. An option to improve this scenario is to identify molecular biomarkers in the early stage of the disease, or even before the disease onset. Blood-based liquid biopsies are a promising, non-invasive way to achieve this goal and there are an increasing number of studies which investigate their potential application in bone cancer diagnosis, prognosis and personalised therapy. This review summarises the interplay between clinical and molecular aspects of the three main bone sarcomas, alongside biomarker discovery and promising applications of liquid biopsy in each tumour context.
DOI : 10.1016/j.critrevonc.2021.103340
Anahtar Kelimeler : Bone sarcomas, Osteosarcoma, Chondrosarcoma, Ewing sarcoma, Liquid biopsy
ISSN: 1040-8428
Cilt: 162
Sayfa: 103340
8
Generation of iPS cell lines from schizophrenia patients using a non-integrative method
Dergi Makalesi

Dergi Makalesi
Open Access
Kaynak :
Directory of Open Access Journals (DOAJ)
Dergi:
Stem Cell Research
Yazar(lar) :
Jaroslaw Sochacki , Sylvie Devalle , Marcelo Reis , Renata de Moraes Maciel , Bruna da Silveira Paulsen , Helena Brentani , Paulo Silva Belmonte-de-Abreu , Stevens Rehen
Yıl :
2016
Sylvie Devalle
Yayıncı Web Sitesi
 Piri Tam Metin Bulucu
Kaydet Alıntıla
Skin biopsies were collected from three male patients (age 45, 44 and 44) with clinically diagnosed schizophrenia. The patients were diagnosed according to DSM-5 criteria by a trained psychiatrist. Dermal fibroblast cell lines were established and expanded for subsequent reprogramming procedures. Induced pluripotent stem (iPS) cells were derived using the integration-free CytoTune®-iPS 2.0 Sendai Reprogramming Kit, containing Sendai virus particles of the four Yamanaka factors Oct3/4, Sox2, Klf4 and c-Myc.
DOI : 10.1016/j.scr.2016.05.017
Cilt: 17
Sayı: 1
Sayfa: 97 - 101
Sınıflandırma :
9
Characterization of a human induced Pluripotent Stem (iPS) cell line (INCABRi002-A) derived from a primary myelofibrosis patient harboring the 5-bp insertion in CALR and the p.W146X mutation in TP53.
Dergi Makalesi

Dergi Makalesi
Open Access
Kaynak :
PubMed
Dergi:
Stem Cell Res
Yazar(lar) :
Cintia E Gomez Limia , Sylvie Devalle , Marcelo Reis , Jaroslaw Sochacki , Rodrigo Madeiro da Costa , Mariana D'Andrea , Telma Padilha , Ilana R Zalcberg , Cristiana Solza , Adelmo Daumas , Stevens Rehen , Martín H Bonamino , Bárbara Monte-Mór
Yıl :
2018
Sylvie Devalle
Piri Tam Metin Bulucu
Kaydet Alıntıla
Primary myelofibrosis (PMF) is a hematological malignancy characterized by activation of the JAK/STAT pathway and risk of leukemic transformation. In this study, we generated an induced Pluripotent Stem (iPS) cell line derived from a 65-year old male PMF patient carrying the 5-pb insertion in the CALR gene (CALRins5) and the c.437 G > A mutation in the TP53 gene (p.W146X). The newly derived PMF3.17 iPS cell line harbors the original mutations and was characterized as bona fide iPS. Resource table.
DOI : 10.1016/j.scr.2018.09.012
Sınıflandırma :
10
Generation and characterization of a human induced pluripotent stem (iPS) cell line derived from an acute myeloid leukemia patient evolving from primary myelofibrosis carrying the CALR 52 bp deletion and the ASXL1 p.R693X mutation
Dergi Makalesi

Dergi Makalesi
Open Access
Kaynak :
Directory of Open Access Journals (DOAJ)
Dergi:
Stem Cell Research
Yazar(lar) :
Cintia E. Gomez Limia , Sylvie Devalle , Marcelo Reis , Jaroslaw Sochacki , Mayra Carneiro , Rodrigo Madeiro da Costa , Mariana D'Andrea , Telma Padilha , Ilana R. Zalcberg , Cristiana Solza , Adelmo Daumas , Stevens Rehen , Bárbara Monte-Mór , Martín H. Bonamino
Yıl :
2017
Sylvie Devalle
Yayıncı Web Sitesi
 Piri Tam Metin Bulucu
Kaydet Alıntıla
Peripheral blood sample was donated by a 61 years old female patient diagnosed with acute myeloid leukemia secondary to a primary myelofibrosis harboring the 52-bp deletion in the CALR gene (c.1092_1143del, p.L367fs*46) and the R693X mutation in the ASXL1 gene (c.2077C>T, p.R693X). CD34+ cells were isolated from the sample and subjected to the reprogramming procedure by using the Sendai virus carrying the reprogramming factors Oct3/4, Sox2, Klf4 and c-Myc. iPS colonies generated retained the original mutations and displayed all the features of bona fide iPS cells.
DOI : 10.1016/j.scr.2017.08.006
Cilt: 24
Sayı: C
Sayfa: 16 - 20
Sınıflandırma :
11
Correction: Frequent MAGE Mutations in Human Melanoma
Araştırma Makalesi

Araştırma Makalesi
Open Access
Kaynak :
PLOS - Public Library of Science
Dergi:
Public Library of Science
Yazar(lar) :
Andrew J. Simpson , Robert L. Strausberg , Jonathan Cebon , Xin Lu , Lloyd J. Old , Benoit J. Van den Eynde , Francis Brasseur , Kunle Odunsi , Tanja Pejovic , Cailian Liu , Jedd D. Wolchok , Daniel Speiser , Olivier Michielin , Anna Pagotto , Ute F. Röhrig , Sylvie Devalle , Suzanne Svobodová , Brian J. Stevenson , Donata Rimoldi , Qi Zhao , Otavia L. Caballero
Yıl :
2010
Sylvie Devalle
Piri Tam Metin Bulucu
Kaydet Alıntıla
DOI : 10.1371/annotation/3ee2788f-0c44-429f-85c3-d626c9fedc21
Sınıflandırma :
12
Frequent MAGE Mutations in Human Melanoma
Araştırma Makalesi

Araştırma Makalesi
Open Access
Kaynak :
PLOS - Public Library of Science
Dergi:
Public Library of Science
Yazar(lar) :
Andrew J. Simpson , Robert L. Strausberg , Jonathan Cebon , Xin Lu , Lloyd J. Old , Benoit J. Van den Eynde , Francis Brasseur , Kunle Odunsi , Tanja Pejovic , Cailian Liu , Jedd D. Wolchok , Daniel Speiser , Olivier Michielin , Anna Pagotto , Ute F. Röhrig , Sylvie Devalle , Suzanne Svobodová , Brian J. Stevenson , Donata Rimoldi , Qi Zhao , Otavia L. Caballero
Yıl :
2010
Sylvie Devalle
Tam Metin
Kaydet Alıntıla
Background Cancer/testis (CT) genes are expressed only in the germ line and certain tumors and are most frequently located on the X-chromosome (the CT-X genes). Amongst the best studied CT-X genes are those encoding several MAGE protein families. The function of MAGE proteins is not well understood, but several have been shown to potentially influence the tumorigenic phenotype. Methodology/Principal Findings We undertook a mutational analysis of coding regions of four CT-X MAGE genes, MAGEA1, MAGEA4, MAGEC1, MAGEC2 and the ubiquitously expressed MAGEE1 in human melanoma samples. We first examined cell lines established from tumors and matching blood samples from 27 melanoma patients. We found that melanoma cell lines from 37% of patients contained at least one mutated MAGE gene. The frequency of mutations in the coding regions of individual MAGE genes varied from 3.7% for MAGEA1 and MAGEA4 to 14.8% for MAGEC2. We also examined 111 fresh melanoma samples collected from 86 patients. In this case, samples from 32% of the patients exhibited mutations in one or more MAGE genes with the frequency of mutations in individual MAGE genes ranging from 6% in MAGEA1 to 16% in MAGEC1. Significance These results demonstrate for the first time that the MAGE gene family is frequently mutated in melanoma.
DOI : 10.1371/journal.pone.0012773
Anahtar Kelimeler : Melanomas, Nonsense mutation, Mutation, Somatic mutation, Mutation databases, Breast cancer, Polymerase chain reaction, Melanoma cells
Sınıflandırma :
13
Short and long TNF‐alpha exposure recapitulates canonical astrogliosis events in human‐induced pluripotent stem cells‐derived astrocytes
Dergi Makalesi

Dergi Makalesi
Kaynak :
Wiley Online Library
Yazar(lar) :
Pablo Trindade , Erick Correia Loiola , Juciano Gasparotto , Camila Tiefensee Ribeiro , Pablo Leal Cardozo , Sylvie Devalle , José Alexandre Salerno , Isis Moraes Ornelas , Pitia Flores Ledur , Fabiola Mara Ribeiro , Ana Lucia Marques Ventura , José Claudio Fonseca Moreira , Daniel Pens Gelain , Lisiane Oliveira Porciúncula , Stevens Kastrup Rehen
Konu :
Cellular and Molecular Neuroscience, Neurology
Yıl :
2020
Sylvie Devalle
Yayıncı Web Sitesi
Tam Metin
Kaydet Alıntıla
Astrogliosis comprises a variety of changes in astrocytes that occur in a context‐specific manner, triggered by temporally diverse signaling events that vary with the nature and severity of brain insults. However, most mechanisms underlying astrogliosis were described using animals, which fail to reproduce some aspects of human astroglial signaling. Here, we report an in vitro model to study astrogliosis using human‐induced pluripotent stem cells (iPSC)‐derived astrocytes which replicate temporally intertwined aspects of reactive astrocytes in vivo. We analyzed the time course of astrogliosis by measuring nuclear translocation of NF‐kB, production of cytokines, changes in morphology and function of iPSC‐derived astrocytes exposed to TNF‐α. We observed NF‐kB p65 subunit nuclear translocation and increased gene expression of IL‐1β, IL‐6, and TNF‐α in the first hours following TNF‐α stimulation. After 24 hr, conditioned media from iPSC‐derived astrocytes exposed to TNF‐α exhibited increased secretion of inflammation‐related cytokines. After 5 days, TNF‐α‐stimulated cells presented a typical phenotype of astrogliosis such as increased immunolabeling of Vimentin and GFAP and nuclei with elongated shape and shrinkage. Moreover, ~50% decrease in aspartate uptake was observed during the time course of astrogliosis with no evident cell damage, suggesting astroglial dysfunction. Together, our results indicate that human iPSC‐derived astrocytes reproduce canonical events associated with astrogliosis in a time dependent fashion. The approach described here may contribute to a better understanding of mechanisms governing human astrogliosis with potential applicability as a platform to uncover novel biomarkers and drug targets to prevent or mitigate astrogliosis associated with human brain disorders.
DOI : 10.1002/glia.23786
ISSN: 0894-1491
Sayı: 7
Cilt: 68
Sayfa: 1396-1409
14
A comprehensive promoter landscape identifies a novel promoter for CD133 in restricted tissues, cancers, and stem cells.
e-Kitap

e-Kitap
Open Access
Kaynak :
Internet Archive Books
Dergi:
Frontiers in Genetics
Yayıncı:
Frontiers in Genetics
Yazar(lar) :
Winston Hide , Jonathan Cebon , Elizabeth R Lawlor , Yoshihide Hayashizaki , Piero Carninci , Otavia L Caballero , Sylvie Devalle , Carsten O Daub , Morana Vitezic , Andreas Behren , Craig Gedye , Christopher A Maher , Oliver Hofmann , Ramakrishna Sompallae
Yıl :
2013
Sylvie Devalle
Yayıncı Web Sitesi
Tam Metin
Kaydet Alıntıla
This article is from Frontiers in Genetics, volume 4. Abstract PROM1 is the gene encoding prominin-1 or CD133, an important cell surface marker for the isolation of both normal and cancer stem cells. PROM1 transcripts initiate at a range of transcription start sites (TSS) associated with distinct tissue and cancer expression profiles. Using high resolution Cap Analysis of Gene Expression (CAGE) sequencing we characterize TSS utilization across a broad range of normal and developmental tissues. We identify a novel proximal promoter (P6) within CD133+ melanoma cell lines and stem cells. Additional exon array sampling finds P6 to be active in populations enriched for mesenchyme, neural stem cells and within CD133+ enriched Ewing sarcomas. The P6 promoter is enriched with respect to previously characterized PROM1 promoters for a HMGI/Y (HMGA1) family transcription factor binding site motif and exhibits different epigenetic modifications relative to the canonical promoter region of PROM1.
DOI : doi:10.3389/fgene.2013.00209
Cilt :4
ISSN :1664-8021
Sınıflandırma :
15
Neuromechanisms of SARS-CoV-2: A Review
Dergi Makalesi

Dergi Makalesi
Open Access
Kaynak :
Directory of Open Access Journals (DOAJ)
Dergi:
Frontiers in Neuroanatomy
Yazar(lar) :
Marcos F. DosSantos , Marcos F. DosSantos , Marcos F. DosSantos , Sylvie Devalle , Veronica Aran , Daniela Capra , Daniela Capra , Daniela Capra , Natália Roberta Roque , Juliana de Mattos Coelho-Aguiar , Tânia Cristina Leite de Sampaio e Spohr , Tânia Cristina Leite de Sampaio e Spohr , Janice Gonçalves Subilhaga , Cláudia Maria Pereira , Isabella D'Andrea Meira , Isabella D'Andrea Meira , Isabella D'Andrea Meira , Paulo Niemeyer Soares Filho , Vivaldo Moura-Neto , Vivaldo Moura-Neto , Vivaldo Moura-Neto , Vivaldo Moura-Neto , Vivaldo Moura-Neto
Yıl :
2020
Sylvie Devalle
Yayıncı Web Sitesi
 Piri Tam Metin Bulucu
Kaydet Alıntıla
Recent studies have suggested the neuroinvasive potential of severe acute respiratory coronavirus 2 (SARS-CoV-2). Notably, neuroinvasiveness might be involved in the pathophysiology of coronavirus disease 2019 (COVID-19). Some studies have demonstrated that synapse-connected routes may enable coronaviruses to access the central nervous system (CNS). However, evidence related to the presence of SARS-CoV-2 in the CNS, its direct impact on the CNS, and the contribution to symptoms suffered, remain sparse. Here, we review the current literature that indicates that SARS-CoV-2 can invade the nervous system. We also describe the neural circuits that are potentially affected by the virus and their possible role in the progress of COVID-19. In addition, we propose several strategies to understand, diagnose, and treat the neurological symptoms of COVID-19.
DOI : 10.3389/fnana.2020.00037
Anahtar Kelimeler : SARS-CoV-2, COVID-19, central nervous system, peripheral nervous system, anosmia, dysgeusia
ISSN: 1662-5129
Cilt: 14
Sınıflandırma :
16
Zika virus infection leads to mitochondrial failure, oxidative stress and DNA damage in human iPSC-derived astrocytes
Dergi Makalesi

Dergi Makalesi
Open Access
Kaynak :
Directory of Open Access Journals (DOAJ)
Dergi:
Scientific Reports
Yazar(lar) :
Pítia Flores Ledur , Karina Karmirian , Carolina da Silva Gouveia Pedrosa , Leticia Rocha Quintino Souza , Gabriela Assis-de-Lemos , Thiago Martino Martins , Jéssica de Cassia Cavalheiro Gomes Ferreira , Gabriel Ferreira de Azevedo Reis , Eduardo Santos Silva , Débora Silva , José Alexandre Salerno , Isis Moraes Ornelas , Sylvie Devalle , Rodrigo Furtado Madeiro da Costa , Livia Goto-Silva , Luiza Mendonça Higa , Adriana Melo , Amilcar Tanuri , Leila Chimelli , Marcos Massao Murata , Patrícia Pestana Garcez , Eduardo Cremonese Filippi-Chiela , Antonio Galina , Helena Lobo Borges , Stevens Kastrup Rehen
Yıl :
2020
Sylvie Devalle
Yayıncı Web Sitesi
 Piri Tam Metin Bulucu
Kaydet Alıntıla
Abstract Zika virus (ZIKV) has been extensively studied since it was linked to congenital malformations, and recent research has revealed that astrocytes are targets of ZIKV. However, the consequences of ZIKV infection, especially to this cell type, remain largely unknown, particularly considering integrative studies aiming to understand the crosstalk among key cellular mechanisms and fates involved in the neurotoxicity of the virus. Here, the consequences of ZIKV infection in iPSC-derived astrocytes are presented. Our results show ROS imbalance, mitochondrial defects and DNA breakage, which have been previously linked to neurological disorders. We have also detected glial reactivity, also present in mice and in post-mortem brains from infected neonates from the Northeast of Brazil. Given the role of glia in the developing brain, these findings may help to explain the observed effects in congenital Zika syndrome related to neuronal loss and motor deficit.
DOI : 10.1038/s41598-020-57914-x
ISSN: 2045-2322
Cilt: 10
Sayı: 1
Sayfa: 1 - 14
Sınıflandırma :
Biyotıp %100
Viroloji %100
17
Distinct patterns of somatic alterations in a lymphoblastoid and a tumor genome derived from the same individual.
e-Kitap

e-Kitap
Open Access
Kaynak :
Internet Archive Books
Dergi:
Nucleic Acids Research
Yayıncı:
Nucleic Acids Research
Yazar(lar) :
Anamaria A Camargo , Sandro J de Souza , Andrew J G Simpson , Robert L Strausberg , Marco Antonio Zago , Bing Ren , Ana Tereza R Vasconcelos , Wilson A da Silva , Samuel Levy , Ewen F Kirkness , Lucila Ohno-Machado , Marcelo G de Paula , Thiago Y K Oliveira , Rodrigo G M A de Sousa , Renato G Pedigoni , Israel Tojal , Daniel G Pinheiro , Samantha Kuan , Zhen Ye , Luiz G Almeida , Sylvie Devalle , Lee Edsall , Ana Paula M Silva , Anna Christina M Salim , Marisa F Nicolas , Alexandra L Gerber , Fabio C P Navarro , Jorge E de Souza , Otavia L Caballero , Qi Zhao , Raphael B Parmigiani , Pedro A F Galante
Yıl :
2011
Sylvie Devalle
Yayıncı Web Sitesi
Tam Metin
Kaydet Alıntıla
This article is from Nucleic Acids Research, volume 39. Abstract Although patterns of somatic alterations have been reported for tumor genomes, little is known on how they compare with alterations present in non-tumor genomes. A comparison of the two would be crucial to better characterize the genetic alterations driving tumorigenesis. We sequenced the genomes of a lymphoblastoid (HCC1954BL) and a breast tumor (HCC1954) cell line derived from the same patient and compared the somatic alterations present in both. The lymphoblastoid genome presents a comparable number and similar spectrum of nucleotide substitutions to that found in the tumor genome. However, a significant difference in the ratio of non-synonymous to synonymous substitutions was observed between both genomes (P = 0.031). Protein–protein interaction analysis revealed that mutations in the tumor genome preferentially affect hub-genes (P = 0.0017) and are co-selected to present synergistic functions (P < 0.0001). KEGG analysis showed that in the tumor genome most mutated genes were organized into signaling pathways related to tumorigenesis. No such organization or synergy was observed in the lymphoblastoid genome. Our results indicate that endogenous mutagens and replication errors can generate the overall number of mutations required to drive tumorigenesis and that it is the combination rather than the frequency of mutations that is crucial to complete tumorigenic transformation.
DOI : doi:10.1093/nar/gkr221
Cilt :39
ISSN :0305-1048

Yayın Yılı

Sınırla

Doküman Türü
Tümü

Veri Tabanı
Tümü

Dergi İsmi
Tümü

Disiplin
Tümü

Disipline Göre İlgililik

Alt Disiplin
Tümü

Konu
Tümü

Yayıncı
Tümü

Lisan
Tümü

X
Filtreler