Two common urban greening trees, ash (Fraxinus chinensis Roxb.) and maple (Acer truncatum Bunge.), were planted in arranged pervious and impervious land pavements to clarify the response in the photosynthetic processes of the urban tree under different types of pavement. Leaf light and CO2 response curves of the net photosynthetic rate were constructed based on in situ measurements in the 4th year after planting, and additional photosynthetic parameters were obtained. The surface temperature and soil temperature significantly increased while the soil moisture significantly decreased in the land pavement, and these changes varied with types of pavement. The light-saturated net photosynthetic rates of both ash and maple, the saturated intercellular CO2 concentration of ash, and the light saturation point, CO2-saturated net photosynthetic rate and maximum carboxylation rate of maple significantly decreased in impervious pavement, indicating that both the capacity of leaf photosynthesis and utilization of high light and CO2 concentrations were significantly reduced by land pavement. The down-regulation of photosynthesis in the impervious pavement was mainly due to the reduction of available soil water. Photosynthetic parameters of maple showed more sensitivity to the land pavement than those of ash. There was less impact from pervious pavement than impervious pavement on the photosynthetic parameters of ash and maple.
A tandem C(sp2)H and C(sp3)O bond formation catalyzed by iodine(III) reagents generated in situ has been developed. The method shows wide scope under mild conditions and exhibits an unprecedented selectivity profile that can be switched depending on the catalyst employed.
DOI : https://dx.doi.org/10.1002/anie.201407011
Tez türü: article
The main purpose of this paper is to seek a mechanical interpretation of electromagnetic phenomena. We suppose that vacuum is filled with a kind of continuously distributed material which may be called Omega(1) substratum. Further, we speculate that the Omega(1) substratum might behave like a fluid with respect to translational motion of large bod- ies through it, but would still posses elasticity to produce small transverse vibrations. Thus, we propose a visco-elastic constitutive relation of the Omega(1) substratum. Further- more, we speculate that electric charges are emitting or absorbing the Omega(1) substratum continuously and establish a fluidic source and sink model of electric charges. Thus, Maxwell’s equations in vacuum are derived by methods of continuum mechanics based on this mechanical model of vacuum and the singularity model of electric charges.
The tensile behaviour of polyethylene/polypropylene (PE/PP) bicompomnent fibre spun bonded nonwovens has been studied using an environmental scanning electron microscope (ESEM). A tensile stage, mounted in the ESEM, was used to examine the dynamic process of the PE/PP bicompomnent fibre spunbonded nonwovens at different stages of deformation. The visual information obtained through the ESEM provides clear evidence of relevant mechanism of nonwoven deformation. The study shows that the ESEM is a powerful tool for examining the dynamic tensile behaviour of different materials.
Xintong Wang,1,2 Dianzheng An,1 Xiaomeng Liu,3 Xinlei Wang,4 Baosheng Li11Department of Radiation Oncology, Shandong Cancer Hospital Affiliated to Shandong University, Shandong University, Jinan, Shandong, People’s Republic of China; 2Department of Oncology, The Affiliated Hospital of Qingdao University, Qingdao, Shandong, People’s Republic of China; 3University of Jinan, School of Medicine and Life Sciences, Shandong Academy of Medical Sciences, Jinan, Shandong, People’s Republic of China; 4Department of Gastroenterology, Qingdao Hiser Medical Center, Qingdao, Shandong, People’s Republic of ChinaPurpose: Accumulating evidence suggests that microRNAs (miRNAs) possess diverse cellular regulatory roles in radiation responses. In this study, we aimed to identify the role of miR-27a in esophageal squamous cell carcinoma (ESCC) radiosensitivity by exploring the relationship between miR-27a and heat shock protein 90 (Hsp90).Materials and methods: We performed quantitative real-time polymerase chain reaction (qRT-PCR) to detect miR-27a expression in the plasma of ESCC patients and healthy volunteers. The expression of Hsp90 and its key client proteins associated with radioresistance were analyzed by Western blotting. Then, the effects of miR-27a on proliferation, apoptosis, cell cycle and radiosensitivity in ESCC cell lines were determined by CCK-8, flow cytometry, and clonogenic survival assay. We also generated subcutaneous tumors to explore whether miR-27a enhanced radiosensitivity in vivo.Results: In our current study, we found that miR-27a expression was downregulated in the plasma of ESCC patients compared with that of healthy volunteers. Overexpression of miR-27a in ESCC cell lines caused a reduction of Hsp90 mRNA and protein. We also demonstrated that upregulation of miR-27a induced degradation of Hsp90 key client proteins associated with radioresistance. In related functional experiments, miR-27a significantly inhibited growth, increased radiation-induced apoptosis, induced cell cycle arrest in G0/G1 phase and enhanced ESCC radiosensitivity both in vitro and in vivo.Conclusion: From these findings, we concluded that miR-27a may contribute to radiosensitivity by modulating Hsp90 expression. Moreover, miR-27a-based therapy utilized to target Hsp90 could be contemplated as a compelling alternative for sensitize ESCC to radiotherapy with fewer side effects.Keywords: miR-27a, Hsp90, esophageal squamous cell carcinoma, radiosensitivity
Xintong Wang,1 Dianzheng An,1 Xinlei Wang,2 Xiaomeng Liu,3 Baosheng Li1 1Department of Radiation Oncology, Shandong Cancer Hospital Affiliated to Shandong University, Shandong University, Jinan, Shandong, People’s Republic of China; 2Department of Gastroenterology, Qingdao Hiser Medical Center, Qingdao, Shandong, People’s Republic of China; 3University of Jinan, School of Medicine and Life Sciences, Shandong Academy of Medical Sciences, Jinan, Shandong, People’s Republic of China Purpose: Extracellular Hsp90α (eHsp90α) is known to be involved in tumor invasiveness and metastasis, and its prognostic value in many kinds of tumors has been identified. We aimed to evaluate the clinical and functional role of eHsp90α in esophageal squamous cell carcinoma (ESCC). Patients and methods: A total of 193 patients with newly diagnosed ESCC were retrospectively evaluated. The relationship between serum Hsp90α levels before treatment and ESCC malignancy of the patients was analyzed. To test the role of eHsp90α in migration and invasion of ESCC cell lines, transwell assay was performed. Western blotting was used to explore the possible mechanism in which eHsp90α promotes ESCC migration and invasion. Results: We found that the serum Hsp90α level before treatment is positively correlated with ESCC malignancy. Moreover, high serum Hsp90α level before treatment was significantly correlated with positive lymph node (LN) metastasis, which is the main prognostic factor for ESCC patients. Meanwhile, we demonstrated that eHsp90α promoted migration and invasion of ECA109 and ECA9706 in vitro. Further investigations revealed that eHsp90α stabilized MMP-2 and promoted epithelial-to-mesenchymal transition evidenced by downregulation of E-cadherin and upregulation of N-cadherin. On the other hand, Hsp90α neutralizing antibody functionally blocked the secreted Hsp90α and reversed those effects. Conclusion: Our findings prove the critical role of eHsp90α in promoting ESCC migration and invasion, indicating it can be not only a promising predictor for ESCC LN status, but also an effective target in ESCC therapeutics, especially in preventing LN metastasis. Keywords: extracellular Hsp90α, esophageal squamous cell carcinoma, lymph node metastasis, migration, invasion, MMP-2
Shu Li, Xi-Peng Wang Shanghai First Maternity and Infant Hospital, Tongji University School of Medicine, Shanghai, People’s Republic of China Abstract: In this study, a novel NGR (Asn-Gly-Arg) peptide-modified liposomal brucine was prepared by using spray-drying method. The surface morphology of the liposomes, encapsulation efficiency and particle size were investigated. The data showed that the addition of NGR did not produce any significant influence on brucine liposomes in terms of particle size or zeta potential. In addition, after 3 months of storage, no dramatic change such as visible aggregation, drug content changes or precipitation in the appearance of NGR-brucine liposomes occurred. The in vitro release results indicated that the release of brucine from NGR liposomes was similar to that of liposomes, demonstrating that the NGR modification did not affect brucine release. The in vitro drug-release kinetic model of NGR-brucine liposomes fitted well with the Weibull’s equation. In vivo, NGR-brucine liposomes could significantly extend the bioavailability of brucine; however, there was no significant difference observed in the pharmacokinetic parameters between liposomes and NGR liposomes after intravenous administration. Antitumor activity results showed that NGR-modified liposomes exhibited less toxicity and much higher efficacy in HepG2-bearing mice compared with non-modified liposomes. The enhanced antitumor activity might have occurred because brucine was specifically recognized by NGR receptor on the surface of tumor cells, which enhanced the intracellular uptake of drugs. Keywords: brucine, liposome, NGR, HepG2, in vivo, in vitro
Xinjing Wang, Bilan Li Department of Gynecology, Shanghai First Maternity and Infant Hospital, Tongji Hospital, School of Medicine, Shanghai, People’s Republic of China Abstract: Endometrial carcinoma (EC) is the most common gynecologic malignancy, but the molecular events involved in the development and progression of EC remain unclear. This study aimed to investigate the role of DNA methyltransferase 1 (DNMT1), a member of DNA methyltransferases, in EC. AN3CA cells were transfected with DNMT1 siRNA. The proliferation, cell cycle, and apoptosis of AN3CA cells were evaluated by Cell Counting Kit-8 (CCK-8) assay and flow cytometry. The expression of related genes was detected by polymerase chain reaction and Western blot analysis. Knockdown of DNMT1 inhibited the proliferation, induced apoptosis, and G0/G1 phase arrest of AN3CA cells. Furthermore, knockdown of DNMT1 upregulated the expression of nuclear factor kappa-B-inhibitor alpha (NF-κBIA) and Bax and downregulated the expression of Bcl-2 and CCND1/2 in AN3CA cells. In conclusion, this study provides the first evidence that knockdown of DNMT1 affects the expression of cell cycle- and apoptosis-associated proteins in EC cells, suggesting the potential of DNMT1 in EC therapy. Keywords: DNMT1, endometrial carcinoma, NF-κB, Bcl-2, Bax, CCND1/2
Xiaohan Wang,1 Shuai Miao2 1Department of Anesthesiology, Xuzhou Medical University, Xuzhou, Jiangsu, People’s Republic of China; 2Department of Anesthesiology, The Affiliated Wuxi People’s Hospital of Nanjing Medical University, Wuxi, Jiangsu, People’s Republic of ChinaCorrespondence: Shuai MiaoDepartment of Anesthesiology, The Affiliated Wuxi People’s Hospital of Nanjing Medical University, Wuxi, Jiangsu 214023, People’s Republic of ChinaEmail firstname.lastname@example.orgWe read the observational study “Sufentanil Sublingual Tablet System (SSTS) for the management of postoperative pain after major abdominal and gynecological surgery within an ERAS protocol: an observational study” of Stefano Turi et al.1 published in August 2019, Volume 12, p2313–2319 with great pleasure. I think that the findings detected in this study will contribute to the clinical practice. However, the statistical analyses were not appropriate.View the original paper by Stefano Turi and colleagues
Huidong Wang,1 Xin Wang2 1Geriatric Department, The Fourth Clinical College of Harbin Medical University, Harbin, 2Geriatric Department, Daqing Oilfield General Hospital, Daqing, Heilongjiang, People’s Republic of China Objective: This study was designed to investigate the efficacy and safety outcomes of ticagrelor in comparison with clopidogrel on a background of aspirin in elderly Chinese patients with acute coronary syndrome (ACS).Patients and methods: A double-blinded, randomized controlled study was conducted, and 200 patients older than 65 years with the diagnosis of ACS were assigned 1:1 to take ticagrelor or clopidogrel. The course of treatment was required to continue for 12 months.Results: The median age of the whole cohort was 79 years (range: 65–93 years), and females accounted for 32.5% (65 patients). Baseline characteristics and clinical diagnosis had no significant difference between patients taking ticagrelor and clopidogrel; they were also balanced with respect to other treatments (P>0.05 for all). The risk of cardiovascular death was significantly lower in patients taking ticagrelor compared with clopidogrel, as was the risk of myocardial infarction (P<0.05 for all); there was no difference in the risk of stroke (P>0.05). Ticagrelor was more effective than clopidogrel in decreasing the primary efficacy end point (cardiovascular death, myocardial infarction, and stroke, P<0.05). The all-cause mortality was not significantly different between patients taking ticagrelor and clopidogrel (P>0.05). The difference in the risk of bleeding, platelet inhibition and patient outcomes major bleeding (life-threatening bleeding and others), and platelet inhibition and patient outcomes minor bleeding was not evident between patients taking ticagrelor and clopidogrel (P>0.05 for all).Conclusion: The current study in elderly Chinese patients with ACS demonstrated that ticagrelor reduced the primary efficacy end point at no expense of increased bleeding risk compared with clopidogrel, suggesting that ticagrelor is a suitable alternative for use in elderly Chinese patients with ACS. Keywords: acute coronary syndrome, Chinese elderly, clopidogrel, ticagrelor
Xin Wang,1,* Shikui Li2,* 1Intensive Care Unit, 2Cardiothoracic Surgery, Daqing Oilfield General Hospital, Daqing, Heilongjiang, People’s Republic of China *These authors contributed equally to this work Aim: As a primary cause of death not only in Western countries but also in the People’s Republic of China, sepsis is diagnosed as abnormal organ functions as a result of a disordered response to a severe infection. This study was designed to assess the effect of small-dose levosimendan without a loading dose on mortality rates and organ functions in Chinese elderly patients with sepsis.Methods: Following a prospective, randomized, and double-blinded design, 240 Chinese elderly patients with sepsis shock were admitted to the intensive care unit (ICU). All patients were randomly and evenly assigned into a levosimendan group (number of patients =120) and a control group (number of patients =120). The control group underwent standard care, and the levosimendan group was administered levosimendan in addition to standard care.Results: All participants, comprising 134 males (55.8%) and 106 females (44.2%), were 70 (67–73) years old. Baseline characteristics, preexisting illnesses, initial infections, organ failures, and additional agents and therapies showed no significant difference between the two groups (P>0.05 for all). There were no significant differences in mortality rates at 28 days, at ICU discharge, and at hospital discharge between the two groups (P>0.05 for all). The number of days of ICU and hospital stay in the levosimendan group was significantly less than for those in the control group (P<0.05 for all). Mean daily total sequential organ failure assessment score and all organ scores except the cardiovascular scores showed no significant difference between the two groups (P>0.05 for all). Cardiovascular scores in the levosimendan group were significantly higher than those in the control group (P<0.05 for all).Conclusion: Small-dose levosimendan could not reduce the mortality rates or enhance the respiratory, liver, renal, and coagulation functions, but could shorten the days of ICU and hospital stay, and improve the cardiovascular function, which suggests that small-dose levosimendan is valuable for Chinese elderly patients with sepsis. Keywords: Chinese elderly, levosimendan, mortality rate, organ function, sepsis, small-dose
Anahtar Kelimeler :
Chinese elderly, levosimendan, mortality rate, organ function, sepsis, small-dose.
Cilt: 12 Sayfa: 917 - 921
This paper uses dual-model observations of the 118 stations from October 11th to November 27th, 2016 to establish the quasi real time Beidou Regional Ionospheric Map (RIMC), GPS Regional Ionospheric Map (RIMG) and the combined Regional Ionospheric Map (RIMM). The precise weights of each Beidou and GPS observations are determined by using the Helmert variance component estimation method. Then the DCBs of GPS satellites are compared with that published by CODE. The grid points of the areas where the IPPs covers are selected to further analyze the accuracy of the RIMM by comparing with the RIMC, RIMG and CODE GIM of DoY 287-332, 2016. The experimental results show that the accuracy of the RIMM improves about 0.3 TECU after adding the GPS observations.
Anahtar Kelimeler :
Beidou, Global Ionosphere Map, GPS, Total Electron Content, Article
Sayfa: 1125-1131 ISSN: 0975-1033 (Online); 0379-5136 (Print)
Xi Wang, Fan Wang, Minzhi Chen, Xiaona Wang, Jin Zheng, Aimei Qin Department of Geriatric Cardiology, Chinese PLA General Hospital, Beijing, China Purpose: Increased variability in blood pressure (BP) is known to be closely associated with the development, progression and severity of renal damage in patients with chronic kidney disease. However, little is known about the association of BP variability (BPV) with the decline of renal function in elderly hypertensive patients with well-controlled BP. We, therefore, aimed to investigate the association between BPV and glomerular filtration rate in hypertensive elderly (age >60 years) and very elderly (age >80 years) male patients with BP controlled within the normal range by antihypertensive therapy.Patients and methods: This study involved 484 hospitalized elderly male hypertensive patients with BP controlled within the normal range by antihypertensive therapy. BPV was defined as the SD from mean BP over a 24 h period. Renal function was estimated by estimated glomerular filtration rate (eGFR) which was calculated by the Chinese modified Modification of Diet in Renal Disease Equation. Participants were divided into three groups according to their eGFR data. Multivariate linear regression was then used to analyze the correlation between eGFR and BPV.Results: The 24 h systolic BP (SBP) variability increased as eGFR decreased. There was no significant difference in 24 h SBP variability when compared between elderly and very elderly hypertensive patients. Multivariate linear regression analysis showed that SBP variability demonstrated a negative linear relationship with eGFR (P<0.05) after adjustment for potential confounding factors.Conclusion: Among the parameters of 24 h ambulatory BP monitoring, 24 h SBP variability is the only independent risk factor for a decline in renal function in elderly and very elderly male hypertensive patients with well-controlled BP. Keywords: hypertension, elderly male, blood pressure variability, estimated glomerular filtration rate
Xingqiang Wang, Shanshi Wang, Xiaolong Li, Shigang Jin, Feng Xiong, Xin Wang Department of Neurosurgery, People’s Hospital of Rizhao, Jining Medical University, Rizhao, China Abstract: To date, β-catenin has been reported to be implicated in mediating the epithelial-mesenchymal transition (EMT) in a variety of human cancers, which can be triggered by EGF. However, the mechanisms underlying EGF-β-catenin pathway-induced EMT of glioblastoma multiforme (GBM) have not been reported previously. In the present study, immunohistochemistry, reverse transcription polymerase chain reaction, and Western blot were applied to investigate the effect of EGF-β-catenin pathway on EMT of GBM. Here, we identified that β-catenin mRNA and protein levels were up-regulated in GBM tissues and four kinds of glioblastoma cell lines, including T98G, A172, U87, and U251 cells, compared with normal brain tissue and astrocytes. In U87 cell line, inhibition of β-catenin by siRNA suppressed EGF-induced proliferation, migration, invasiveness, and the expression of EMT activators (Snail and Slug). In addition, the expression of epithelial markers (E-cadherin) was up-regulated and the expression of mesenchymal markers (N-cadherin and MMP9) was down-regulated. Finally, inhibitor of PI3K/Akt signaling pathways inactivated the EGF-β-catenin-induced EMT. In conclusion, β-catenin-EMT pathway induced by EGF is important for GBM progression by the PI3K/Akt pathways. Inhibition of β-catenin leads to suppression of EGF pathway-induced EMT, which provides a new way to treat GBM patients. Keywords: EGF, β-catenin, EMT, GBM
Yunjian Zhang,1 Ling Luo,1 Xiaofang Wang,1 Xiaoyang Liu,1 Xiaoyan Wang,1 Yi Ding2 1Division of Pulmonary and Critical Care Medicine, Department of Medicine, 2Department of Pathology, Jishuitan Hospital, Fourth Medical College of Peking University, Beijing, People’s Republic of China Abstract: Mesalazine-induced eosinophilic pneumonia has been rarely reported. We reported a case of mesalazine-induced eosinophilic pneumonia in a 56-year-old female who took mesalazine without a prescription for suspected ulcerative colitis. She had an elevated eosinophil count in peripheral blood and bronchoalveolar lavage fluid. Eosinophil infiltration was also noted in bone marrow aspirates. Chest radiograph and computed tomography demonstrated bilateral upper lung predominant infiltrates and spirometry showed a restrictive ventilatory defect with a reduced diffusion capacity. The patient recovered after cessation of mesalazine therapy. Mesalazine-induced lung damage should be considered in patients who develop unexplained respiratory symptoms while taking this agent. Keywords: mesalazine, pneumonia, eosinophil, colitis
YuanYing Ma,1 LiSan Zhang,2 Xian Wang,3 Liqian Qiu,1 Therese Hesketh,4,5 Xinyu Wang6 1Department of Women’s Healthcare, Women’s Hospital, Zhejiang University School of Medicine, Zhejiang Province, People’s Republic of China; 2Department of Neurology, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Zhejiang Province, People’s Republic of China; 3Department of Big Data Analysis, Hangzhou Maicim Medical Technology Co., Ltd, Zhejiang Province, People’s Republic of China; 4Centre for Global Health, Zhejiang University, Zhejiang Province, People’s Republic of China; 5Institute for Global Health, University College London; London, UK; 6Department of Obstetrics and Gynaecology, Women’s Hospital, Zhejiang University School of Medicine, People’s Republic of ChinaCorrespondence: Xinyu Wang; Therese Hesketh Email email@example.com; firstname.lastname@example.orgBackground and Aim: Maternal near-miss (MNM) surveillance has been developed to identify severe complications in pregnancy since 2011 in China. However, very little is known about MNM in China. This study aims to explore the prevalence of MNM, the risk factors, and perinatal outcomes using the WHO near-miss approach in a developed Chinese province.Materials and Methods: We used data from China’s National Maternity Near Miss Obstetrics Surveillance System for the period 2012 to 2017, which included 18 hospitals in Zhejiang Province. Chi-squared tests were used to compare the socio-demographic factors, obstetric complications and perinatal outcomes between women with and without MNM. Logistic regression was used to examine the independent risk factors for MNM.Results: A total of 612,264 pregnant women were recruited. There were 3208 MNM cases and 34 maternal deaths. The MNM incidence ratio was low at 5.9 per 1,000 live births, with an MNM mortality ratio of 98:1 and mortality index of 1.1%. Among 3208 women with MNM, postpartum hemorrhage was the commonest cause at 76.3% followed by severe anemia at 23.7% and placenta previa at 23.0%. Embolism was identified as having the highest risk for MNM (AOR 46.0; 95% CI 19.1– 110.7), followed by postpartum hemorrhage (AOR 41.0; 95% CI 35.7– 47.0), and severe anemia (AOR 36.6; 95% CI 16.0– 84.1). MNM cases were significantly associated with severe perinatal outcome, including premature birth, low birth weight, multiple fetuses, stillbirth rates and neonatal mortality.Conclusion: Overall near-miss indicators suggested a relatively high quality of maternal health care in a developed province of China. The identified risk factors may be helpful in developing targeted interventions for improving maternal safety.Keywords: maternal near-miss, adverse perinatal outcomes, clinical audit, high-risk pregnancy, pregnancy complication
Jiahui Wan,1,2 Daiqian Deng,3 Xiuli Wang,1,4 Xiaojin Wang,1 Shijun Jiang,1,5 Rongjun Cui11Department of Biochemistry and Molecular Biology, Mudanjiang Medical University, Mudanjiang, Heilongjiang, People’s Republic of China; 2Department of Clinical Laboratory, Harbin Public Security Hospital, Harbin, Heilongjiang, People’s Republic of China; 3Department of Biology, Mudanjiang Medical University, Mudanjiang, Heilongjiang, People’s Republic of China; 4Department of Clinical Laboratory, The Seventh Hospital in Qiqihar, Qiqihar, Heilongjiang, People’s Republic of China; 5Department of Clinical Laboratory, Daqing Medical College, Daqing, Heilongjiang, People’s Republic of ChinaBackground: Long non-coding RNAs (lncRNAs) play an important role in the pathogenesis of multiple tumors. However, the roles of lncRNAs during colon adenocarcinoma and cancer progression remain unclear. This study aimed identify new lncRNAs that act as molecular markers for the prevention and diagnosis of colon adenocarcinoma.Methods: RNA sequencing (RNA-Seq) data associated with colon adenocarcinoma were retrieved from the Cancer Genome Atlas (TCGA). Biological processes in Gene Ontology (Go) and the Kyoto Encyclopedia of Genomes (KEGG) were searched for pathways at the significance level. The expression of LINC00491 and its downstream targets were assessed by real-time PCR, Western blotting and dual-luciferase assays. Biological functions of LINC00491 during cell proliferation, migration and invasion were assessed using CCK-8, colony formation assays, wound healing, and transwell invasion assays in colon adenocarcinoma HT-29 and HCT116 cells.Results: Bioinformatics analysis with the TCGA colon adenocarcinoma dataset showed that LINC00491 was significantly up-regulated in colon adenocarcinoma. Furthermore, we found that LINC00491 positively regulates SERPINE1 expression through sponging miR-145 and promoting the proliferation, migration, and invasion of colon adenocarcinoma cells, thus playing an oncogenic role during colon adenocarcinoma pathogenesis.Conclusion: LINC00491 functions as a ceRNA to promote SERPINE1 expression by sponging miR-145. LINC00491 serves as a therapeutic target and prognostic biomarker in colon adenocarcinoma.Keywords: LINC00491, colon adenocarcinoma, competing endogenous RNA, cell proliferation, migration, invasion
Rong Qiang,1,2 Na Cai,2 Xiaobin Wang,2 Lin Wang,2 Ke Cui,2 Xiang Wang,1 Xu Li11Center for Translational Medicine, The First Affiliated Hospital of Xi’an Jiaotong University, 2Medical Heredity Research Center, Northwest Women’s and Children’s Hospital, Shaanxi, People’s Republic of ChinaAbstract: MLL protein genes encode a family of crucial transcription factors that play a key role in multiple cancer development. The functions of different MLL proteins have not been definitively studied. MLL1 is a histone methyltransferase that mediates histone H3 lysine 4, and it has been found to have aberrant expression in several tumors. However, the function of MLL1 in cervical carcinoma is little known. We used tissue analysis, cell culture experiments, and molecular profiling to investigate the mechanism of MLL1 in cervical carcinoma development. We report here that MLL1 is overexpressed in cervical carcinoma tissues and cell lines, and its overexpression is correlated with the tumor grade. Through FACScan flow cytometry assay, we found that MLL1 promotes cell proliferation by promoting the G1/S transition through transcriptional activation of CCND1 in cervical carcinoma cells. Furthermore, we utilized co-immunoprecipitation and glutathione S-transferase pull-down assays to identify β-catenin as the transcription partner for MLL1 and demonstrated that MLL1 and β-catenin act in synergy in the transcriptional activation of CCND1 in cervical carcinoma cells. In addition, transwell assay and anchorage-independent cell growth assay also revealed that MLL1 promotes metastasis of cervical carcinoma cells through interaction with β-catenin. Our study not only demonstrated a role for MLL1 in the proliferation and metastasis of cervical carcinoma cells but also revealed the interaction of MLL1 with β-catenin to play a different role. Keywords: MLL1, cell cycle, β-catenin, metastasis
Zhibo Dang,1,2,* Xiaoli Liu,1,* Xinhui Wang,1 Mengge Li,1,2 Yuyong Jiang,1 Xianbo Wang,1 Zhiyun Yang1 1Center of Integrative Medicine, Beijing Ditan Hospital, Capital Medical University, Chaoyang District, Beijing 100015, People’s Republic of China; 2First Clinical Medical College, Beijing University of Chinese Medicine, Chaoyang District, Beijing 100029, People’s Republic of China *These authors contributed equally to this work Abstract: A systematic review and meta-analysis of previous randomized controlled trials of traditional Chinese medicine (TCM) supporting Qi and enriching blood in the treatment of cancer related anemia (CRA) in patients not receiving chemoradiotherapy were conducted. A total of 13 randomized controlled trials were included. Compared with the control group, better improvement was found for the level of hemoglobin (mean difference=4.57, 95% CI [1.38, 7.76], P=0.005) and overall therapeutic effect (risk ratio [RR]=1.31, 95% CI [1.18, 1.46], P<0.000) in the TCM groups. The incidence of related adverse events was not increased in the TCM groups (RR=0.54, 95% CI [0.29, 0.99], P=0.05). However, due to the relatively low quality and the small sample sizes of the included studies, the results should be interpreted with a degree of caution. Nevertheless, TCM with the role of supporting Qi and enriching blood may be a safe and effective treatment for CRA in patients not receiving chemoradiotherapy and might be considered as an alternative treatment to conventional western medicine including iron supplements and erythropoietin. Keywords: traditional Chinese medicine, supporting Qi and enriching blood, cancer related anemia, randomized controlled trial, meta-analysis
Anahtar Kelimeler :
Traditional Chinese medicine (TCM), supporting Qi and enriching blood, cancer related anemia (CRA), randomized controlled trial, meta-analysis
ISSN: 1177-8881 Cilt: 13 Sayfa: 221 - 230
Mechanoreceptive neurons with or without taste responsiveness were recorded in the cortical taste area (CTA) of rats every 50 or 100 µm along an electrode track made as perpendicular to the surface as possible. Three groups of mechanoreceptive neurons were recognized based on the adequate stimulus, i.e., low-threshold mechanoreceptive (n=16), nociceptive-specific (n=48), and wide-dynamic range neurons (n=392). Except for nine neurons, almost all had receptive fields (RFs) in the oral cavity (n=447). They were categorized into three RF types: those with RFs only in the oral cavity (OC type; n=23), those with RFs both in the oral cavity and on the lip (OL type; n=44) and those with RFs in the oral cavity and on the external surface of the body (WB type; n=380), e.g., tail. Neurons with inhibitory RFs were often located in the infragranular layers. Several neurons with the same receptive features were sequentially recorded along the track, suggesting the presence of columnar organization. The diameter of the possible functional column was the largest (mean 113.85 µm) for WB type neurons, but smaller in the other two types (mean 85 µm for the OC type and 62.5 µm for the OL type). Neurons were segregated according to the adequate stimulus within the column for WB type neurons. Taste-responsive mechanoreceptive neurons (n=33) were recorded at 15 tracks, and two taste neurons were sequentially recorded in five cases, in three of which two successive neurons sharing the best stimulus were recorded. Taste neurons are possibly arranged in a column with a very small diameter within the large column of mechanoreceptive neurons.
Xiyi Wang,1 Jing Shao,2 Zhihong Ye1,2 1Department of Nursing, Zhejiang University School of Medicine Sir Run Run Shaw Hospital, Hangzhou, People’s Republic of China; 2Faculty of Nursing, Zhejiang University School of Medicine, Hangzhou, People’s Republic of ChinaCorrespondence: Zhihong YeDepartment of Nursing, Zhejiang University School of Medicine Sir Run Run Shaw Hospital, Hangzhou, Zhejiang 310020, People’s Republic of ChinaTel +86-0571-86006608Fax +86 0571-86044817Email email@example.comPurpose: This study aimed to translate the Roy adaptation model concept of adaptation level into measurable concepts when considering the health-related quality of life, social support, and self-efficacy of community-dwelling Chinese adults with metabolic syndrome.Patients and Methods: This was a descriptive cross-sectional study. Data were collected from 144 adults with metabolic syndrome in the suburban area of Hangzhou, China. Correlation analysis was performed to examine the relationships between environmental stimuli (sociodemographic characteristics), adaptation level (health-related quality of life and social support), and prerequisite of behavior (self-efficacy). Regression analysis was applied to test the effect of stimuli on adaptation level and their associations with behavior.Results: Adaptation level was potentially influenced by environmental stimuli and evaluated by the quality of life. Age and education level were related to physiological and psychological function. Besides, family income was related to physiological function. Thus, adaptation level may predict individuals’ behavior towards illness management, with a percentage classification accuracy of 76.40%. The vitality and social functioning of the quality of life were identified as significant correlates of self-efficacy.Conclusion: Our findings demonstrate that individuals’ internal and external resources should be considered when developing nursing interventions to improve patients’ adaptation level. Future research should explore whether facilitating self-efficacy among community-dwelling adults with metabolic syndrome may positively influence an individual’s adaptation process in health management.Keywords: chronic illness, physiological adaptation, psychosocial adaptation, quality of life, social support, self-efficacy
Man Meng,1 Lin Sang,2 Xiangyu Wang3 1Department of Oncology, The Second People’s Hospital of Hefei City Affiliated to Anhui Medical University, Hefei City, Anhui Province, 230000, People’s Republic of China; 2Department of Obstetrics and Gynecology, The Second People’s Hospital of Hefei City Affiliated to Anhui Medical University, Hefei City, Anhui Province, 230000, People’s Republic of China; 3Department of Obstetrics and Gynecology, The Affiliated Hospital of Qingdao University, Qingdao City, Shandong Province, 266003, People’s Republic of ChinaCorrespondence: Xiangyu WangDepartment of Obstetrics and Gynecology, The Affiliated Hospital of Qingdao University, No. 16, Jiangsu Road, Qingdao City, Shandong Province 266003, People’s Republic of ChinaTel +86-15863061517Email firstname.lastname@example.orgPurpose: This study is aimed to investigate the specific regulatory role of S100 calcium binding protein A11 (S100A11) on cervical cancer (CC), and reveal the potential mechanisms relating to Wnt/β-catenin signaling.Patients and methods: The expression of S100A11 in cervical squamous cell carcinoma (CSCC), adjacent non-cancerous, cervical intraepithelial neoplasia (CIN), and normal cervical tissues was detected by quantitative real-time PCR and/or immunohistochemistry. After transfection of pENTER-S100A11 or sh-S100A11-1/sh-S100A11-2, the viability, cell cycle, migration and invasion of C33A or SiHa cells were detected. The tumor volume and tumor weight were measured after injection of transfected C33A cells into mice. The expression of E-caherin (CDH2), N-caherin (CDH1), β-catenin (CTNNB1), and c-Myc (MYC) in C33A and SiHa cells was detected by Western blot.Results: The expression of S100A11 was significantly higher in CSCC tissues than in adjacent non-cancerous, CIN, and normal cervical tissues (P < 0.05). S100A11 expression was positively correlated with the FIGO stage and lymph node metastasis of CSCC patients (P < 0.05). The transfection of pENTER-S100A11 into C33A cells significantly increased the cell viability, the percentage of cells in G2/M phase, the numbers of migratory and invasive cells, as well as the tumor volume and weight in mice (P < 0.05). Overexpression of S100A11 also significantly downregulated E-caherin, and upregulated N-caherin, β-catenin, and c-Myc in C33A cells (P < 0.05). The transfection of sh-S100A11-1/sh-S100A11-2 exhibited the opposite results to that of pENTER-S100A11 on SiHa cells.Conclusion: Overexpression of S100A11 promotes the proliferation, migration, invasion, and epithelial-mesenchymal transition of CC cells, and activates Wnt/β-catenin signaling.Keywords: S100A11, cervical cancer, Wnt/β-catenin signaling, proliferation, migration, invasion
Guangjian Li, Xu Wang, Dihui Ma Department of Neurology and Neuroscience Center, First Hospital of Jilin University, Changchun, People’s Republic of China Background: Vortioxetine is an investigational multimodal antidepressant. We conducted this meta-analysis to assess the efficacy and safety of 10 mg vortioxetine in the treatment of major depressive disorder (MDD). Methods: Randomized controlled trials (RCTs) published in PubMed, Web of Science, Embase, and ClinicalTrials.gov were systematically reviewed to assess the treatment effects and safety profiles of patients with MDD who were treated with 10 mg vortioxetine. The outcome measures included response rate, remission rate, changes from baseline in Montgomery–Asberg Depression Rating Scale (MADRS), Hamilton Rating Scale for Depression (24-items) (HAM-D24), Clinical Global Impression-Severity (CGI-S), and Clinical Global Impression-Improvement (CGI-I) scores. Results were expressed with risk ratio or weighted mean difference with 95% confidence intervals. Pooled results were calculated using a fixed-effects model or a random-effects model according to the heterogeneity among included trials. Results: Six RCTs with a total of 1,801 patients met the inclusion criteria and were included in this meta-analysis. The 10 mg vortioxetine dose significantly increased the response rate and remission rate in the treatment of MDD compared with placebo. Moreover, there was a statistically significant reduction from baseline in the MADRS, HAM-D24, CGI-S, and CGI-I scores with 10 mg vortioxetine vs placebo. The incidence of treatment-emergent adverse events such as nausea, vomiting, constipation, and hyperhidrosis was higher in the 10 mg vortioxetine group than in the placebo group. Conclusion: Vortioxetine 10 mg can significantly increase the response rate and remission rate, and reduce the MADRS, HAM-D24, CGI-S, and CGI-I scores in patients with MDD with an acceptable risk of treatment-emergent adverse events. Further well-conducted, large-scale trials are needed to validate these findings. Keywords: vortioxetine, major depressive disorder, meta-analysis
Jiu-ling Zhao,1,2 Xin Wang,1 Yu-shui Wang1 1Department of Pediatrics, Tianjin Nankai Hospital, 2Nankai Clinical School, Tianjin Medical University, Nankai, Tianjin, People’s Republic of China Background: Mycoplasma pneumoniae pneumonia (MPP) is one of the most common childhood community-acquired pneumonias, and the chest radiograph usually shows bronchial pneumonia, segmental/lobar pneumonia, or segmental/lobar pneumonia with pleural effusion. The imbalance of Th1/Th2 function after Mycoplasma pneumoniae infection is an important immunological mechanism of MPP. In this study, we aimed to evaluate the correlations between Th1/Th2 cytokine profiles and chest radiographic manifestations in MPP children.Patients and methods: A total of 87 children with MPP were retrospectively reviewed in this study. According to the chest radiographic manifestations, they were divided into the following three groups: bronchial MPP group, segmental/lobar MPP group, and segmental/lobar MPP with pleural effusion group. Clinical features and changes in Th1/Th2 cytokines were further analyzed.Results: The incidence of tachypnea and cyanosis was higher in children with segmental/lobar MPP with pleural effusion than in those with segmental/lobar or bronchial MPP. The peak body temperature of segmental/lobar MPP was higher than that of bronchial MPP, and the duration of fever and hospitalization was positively correlated with the severity of MPP. MPP children’s chest radiograph showed a relationship with the changes in Th1/Th2 cytokines. Serum interleukin-4, interleukin-10 (IL-10), interferon-γ, and tumor necrosis factor-α (TNF-α) of segmental/lobar MPP were significantly higher than those of bronchial MPP, and serum IL-10 (cutoff value: 27.25 pg/mL) can be used as a diagnostic predictor for segmental/lobar MPP. Serum TNF-α and interleukin-6 of segmental/lobar MPP with pleural effusion were significantly higher than those of segmental/lobar MPP without pleural effusion. Serum TNF-α (cutoff value: 60.25 pg/mL) can be used as a diagnostic predictor for segmental/lobar MPP with pleural effusion.Conclusion: There were significant correlations between Th1/Th2 cytokine profiles and chest radiographic manifestations in MPP children. Serum IL-10 and TNF-α can be used as an optimal predictor for segmental/lobar MPP and segmental/lobar MPP with pleural effusion, respectively. Keywords: Mycoplasma pneumoniae pneumonia, Th1/Th2, cytokine, chest radiograph
Qinglin Li,1 Meng Zhao,2 Xiaodan Wang1 1Department of Health Care, Nanlou Division, Chinese PLA General Hospital, National Clinical Research Center for Geriatric Diseases, Beijing, China; 2Department of Clinical Data Repository, Chinese PLA General Hospital, Beijing, China Objectives: To compare the differences between the Kidney Disease Improving Global Outcomes (KDIGO) criteria of the 48-hour window and the 7-day window in the diagnosis of acute kidney injury (AKI) in very elderly patients, as well as the relationship between the 48-hour and 7-day windows for diagnosis and 90-day mortality. Patients and methods: We retrospectively enrolled very elderly patients (≥75 years old) from the geriatrics department of the Chinese PLA General Hospital between January 2007 and December 2015. AKI patients were divided into 48-hour and 7-day groups by their diagnosis criteria. AKI patients were divided into survivor and nonsurvivor groups by their outcomes within 90 days after diagnosis of AKI. Results: In total, 652 patients were included in the final analysis. The median age of the cohort was 87 (84–91) years, the majority (623, 95.6%) of whom were male. Of the 652 AKI patients, 334 cases (51.2%) were diagnosed with AKI by the 48-hour window for diagnosis, while 318 cases (48.8%) were by the 7-day window for diagnosis. The 90-day mortality was 42.5% in patients with 48-hour window AKI and 24.2% in patients with 7-day window AKI. Kaplan–Meier curves showed that 90-day mortality was lower in the 7-day window AKI group than in the 48-hour window AKI group (log rank: P<0.001). Multivariate analysis by the Cox model revealed that 48-hour window for diagnosis hazard ratio (HR=1.818; 95% CI: 1.256–2.631; P=0.002) was associated with higher 90-day mortality. Conclusion: The 90-day mortality was higher in 48-hour window AKI than in 7-day window AKI in very elderly patients. The 48-hour KDIGO window definition may be less sensitive. The 48-hour KDIGO window definition is significantly better correlated with subsequent mortality and is, therefore, still appropriate for clinical use. Finding early, sensitive biomarkers of kidney damage is a future direction of research. Keywords: acute kidney injury, AKI diagnosis time, very elderly, short-term mortality
Anahtar Kelimeler :
acute kidney injury, AKI diagnosis time, very elderly, short-term mortality
Cilt: 13 Sayfa: 1151 - 1160
Lingchuan Guo,1,* Shan Huang,1,* Xinwei Wang2 1Department of Pathology, Hospital of Suzhou University, Suzhou, 2Department of Oncology, Tumor Hospital of Jiangsu Province, Nanjing, Jiangsu, China *These authors contributed equally to this work Abstract: Osimertinib, an irreversible EGFR/HER2 inhibitor, has been found to be effective in the cancer cell with EGFR gene mutations in preclinical lung cancer models. However, the effect of osimertinib in colorectal cancer (CRC) cells is unclear. In the present study, we investigated how osimertinib suppresses CRC cells growth and potentiates effects of other chemotherapeutic drugs. We found that p73-mediated osimertinib-induced p53 upregulated modulator of apoptosis (PUMA) expression irrespective of p53 status following PI3K/AKT pathway inhibition in CRC cells. Furthermore, PUMA is required for osimertinib-induced apoptosis. In addition, osimertinib also synergized with 5-FU to induce significant apoptosis via PUMA in CRC cells. These results demonstrated a critical role of PUMA in mediating the anticancer effects of osimertinib and suggest that PUMA induction can be used as an indicator of osimertinib sensitivity. Keywords: PUMA, osimertinib, apoptosis, p73, CRC
Xia Wang,1 Qiongni Chen,2 Min Yang1 1Xiangya School of Nursing, 2Nursing Department, Second Xiangya Hospital, Central South University, Changsha, Hunan, People’s Republic of China Background: This study assessed the effect of expressed emotion (EE) among caregivers of schizophrenia patients on their care burden and the illness rehospitalization rate. Subjects and methods: A total of 64 schizophrenia patients hospitalized for the first time and their key caregivers were recruited. The Chinese version of the Camberwell Family Interview (CFI-CV) was used to evaluate the EE of the key caregivers. A family burden questionnaire was used to evaluate the care burden. The patients’ rehospitalization rate and medication compliance were evaluated by the self-designated criteria. The data collection was carried out at the first meeting in the hospital, at 6 months and 12 months after hospital discharge by using the same instruments. Results: The subjective stress burden and subjective demand burden scores were higher in caregivers before and after discharge with statistical difference between the various observation time points (P<0.05). Significant differences were observed in the rehospitalization rate between patients with high medication adherence and low medication adherence at 12 months (P<0.01) and between patients with high expressed emotion (HEE) and low expressed emotion (LEE; P<0.05). The rehospitalization rate in patients with HEE caregivers was higher than that in those with LEE caregivers. The subjective stress burden scores were statistically significant between HEE and LEE caregivers (P<0.05).Conclusion: HEE is a predictor of rehospitalization rate in schizophrenic patients. The burdens of care scores are high in caregivers of schizophrenic patients. The caregivers with HEE have a high score in burden of care compared with those with LEE. Keywords: expressed emotion, care burden, schizophrenia, rehospitalization rate, caregivers
Mingzhe Zhu,1,* Lei Yang,2,* Xin Wang3 1Department of Obstetrics and Gynecology, Jilin Medical College Affiliated Hospital, Jilin City, Jilin Province 132011, People’s Republic of China; 2Department of Medical Clinic, Yuhuangding Hospital, Yantai City, Shandong Province 264000, People’s Republic of China; 3Department of Obstetrics, Qianjiang Central Hospital of Chongqing, Chongqing 409000, People’s Republic of China*These authors contributed equally to this workCorrespondence: Xin Wang Tel +86- 23-79335226Email email@example.comBackground: Long noncoding RNAs play essential roles in regulating drug resistance in cancers. However, how and whether lncRNA nuclear paraspeckle assembly transcript 1 (NEAT1) could mediate cisplatin resistance in ovarian cancer remain poorly understood.Patients and Methods: Eighteen cisplatin-sensitive and 19 cisplatin-resistant patients with ovarian cancer were recruited. Cisplatin-resistant ovarian cancer cells were used for this study. The expression levels of NEAT1, microRNA (miR)-770-5p and poly adenosine diphosphate-ribose polymerase 1 (PARP1) were detected by quantitative real-time polymerase chain reaction or Western blot. Cisplatin resistance was assessed by the half-maximal inhibitory concentration (IC50) of cisplatin, cell viability and apoptosis using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide, flow cytometry and Western blot, respectively. The target association between miR-770-5p and NEAT1 or PARP1 was investigated by dual-luciferase reporter assay. The xenograft model was used to investigate cisplatin resistance in vivo.Results: NEAT1 expression is elevated in cisplatin-resistant ovarian cancer tissues and cells. Knockdown of NEAT1 repressed cisplatin resistance by decreasing the IC50 of cisplatin, cell viability and increasing apoptosis. MiR-770-5p was bound to NEAT1 and PARP1 was confirmed as a target of miR-770-5p. MiR-770-5p inhibition or PARP1 restoration could abate the effect of NEAT1 silencing on cisplatin resistance in cisplatin-resistant ovarian cancer cells. Moreover, NEAT1 knockdown reduced PARP1 expression by increasing miR-770-5p. Interference of NEAT1 decreased xenograft tumor growth by regulating miR-770-5p and PARP1.Conclusion: Knockdown of NEAT1 inhibited cisplatin resistance in ovarian cancer cells by up-regulating miR-770-5p and down-regulating PARP1, providing a new target for improving the efficacy of cisplatin-based therapy in ovarian cancer.Keywords: ovarian cancer, cisplatin resistance, NEAT1, miR-770-5p, PARP1