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Bronchodilator reversibility in patients with COPD revisited: short-term reproducibility
Dergi Makalesi

Dergi Makalesi
Open Access
Kaynak :
Directory of Open Access Journals (DOAJ)
Dergi:
International Journal of COPD
Yazar(lar) :
Pascoe S , Wu W , Zhu CQ , Singh D
Yıl :
2016
Wu W
Yayıncı Web Sitesi
Kaydet Alıntıla
Steven Pascoe,1 Wei Wu,2 Chang-Qing Zhu,3 Dave Singh4 1GSK, Respiratory Medicines Development Center, 2PAREXEL International, Research Triangle Park, NC, USA; 3GSK, Clinical Statistics (Respiratory), Middlesex, UK; 4Medicines Evaluation Unit, University Hospital of South Manchester NHS Foundation Trust, University of Manchester, Manchester, UK Abstract: Categorization of patients with COPD as reversible or nonreversible to a bronchodilator may change over time. This post hoc analysis aimed to determine if an individual’s reversibility, when treated as a continuous variable, could predict his/her future response to two short-acting bronchodilators: albuterol and ipratropium. The analysis was completed using data from a 4-week, randomized, open-label, two-period crossover study (NCT01691482; GSK study DB2114956). Patients received albuterol (doses: UK =4×100 µg/puff; US =4×90 µg/puff) followed 1 hour later by ipratropium (4×20 µg/puff) or vice versa during treatment Period 1. The order of treatments was reversed during Period 2. Predefined efficacy end points included pre- and post-bronchodilator forced expiratory volume in 1 second. The correlation coefficient between bronchodilator response on Days 1 and 10 was investigated, as well as the correlation between treatment response on Day 1 and the mean treatment response on Days 5–10, for each individual patient. Bronchodilator response to albuterol on Day 1 was strongly correlated with that on Day 10 (r=0.64; n=53). The correlation coefficient of bronchodilator treatment response on Day 1 and Days 5–10 was 0.78 (P<0.001; n=53) and 0.76 (P<0.001; n=54) for albuterol and ipratropium, respectively. A single measurement of the initial bronchodilator response to albuterol or ipratropium was, therefore, highly correlated with the subsequent mean bronchodilator response over 5–10 days, demonstrating its potential usefulness for future treatment decisions. Keywords: bronchodilator responsiveness, FEV1, correlation, short-acting bronchodilators, spirometry
Anahtar Kelimeler : bronchodilator responsiveness, reversibility, short-acting bronchodilators, spirometry
Cilt: 11
Sayfa: 2035 - 2040
Sınıflandırma :
2
Comparison of prognostic values of high-sensitivity cardiac troponin T and N-terminal prohormone brain natriuretic peptide to assess mortality in elderly inpatients
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Dergi Makalesi
Open Access
Kaynak :
Directory of Open Access Journals (DOAJ)
Dergi:
Clinical Interventions in Aging
Yazar(lar) :
Chen JR , Wang Q , Wu W , Zhang SJ
Yıl :
2018
Wu W
Yayıncı Web Sitesi
Kaydet Alıntıla
Jie-Ruo Chen,1 Qing Wang,1 Wei Wu,2 Shao-Jing Zhang1 1Department of Geriatrics, Fuxing Hospital, affiliated to Capital Medical University, Beijing, China; 2General Practice Ward, Fuxing Hospital, affiliated to Capital Medical University, Beijing, China Background: Studies have shown that increases in low-level high-sensitivity cardiac troponin T (hs-cTnT) and N-terminal prohormone brain natriuretic peptide (NT-proBNP) in the elderly population lead to high risk of adverse clinical outcomes, such as mortality. In this study, associations between the two biomarkers and long-term mortality in elderly patients hospitalized for medical conditions other than acute cardiovascular events were investigated. A comparison of the predictive value of hs-cTnT and NT-proBNP for all-cause mortality was conducted. Methods: A cohort of 715 elderly inpatients free of acute cardiovascular events was initially recruited. Based on tertiles of baseline hs-cTnT and NT-proBNP levels, the elderly were arranged into low, middle, and high groups. The mortality of each group was observed and the predictive values of hs-cTnT and NT-proBNP compared. Additionally, all-cause mortality was analyzed for hs-cTnT and NT-proBNP combined. Results: There were 135 (18.9%) all-cause mortality cases identified during follow-up (median 47 months). The upper tertile of hs-cTnT was significantly associated with an increased risk of mortality (HR 3.29, 95% CI 1.85–5.85), even after adjustment for potential confounders. However, there were no significant differences observed in mortality rates among the three NT-proBNP groups after adjustment for potential confounders (HR 1.77, 95% CI 0.83–3.17). Compared to NT-proBNP, hs-cTnT was a better predictor of mortality, as area under curves for hs-cTnT and NT-proBNP at 60 months were 0.712 (95% CI 0.616–0.809) and 0.585 (95% CI 0.483–0.6871), respectively. Also, the combination of the two cardiac biomarkers did not render a better predictive value for mortality than hs-cTnT alone. Conclusion: Unlike hs-cTnT, baseline NT-proBNP levels failed to show an independent association with all-cause mortality in hospitalized elderly without acute cardiovascular events. hs-cTnT provided significant prognostic value for mortality in the study cohort. Keywords: high-sensitivity cardiac troponin T (hs-cTnT), N-terminal prohormone brain natriuretic peptide (NT-proBNP), nonacute cardiovascular events, elderly patients
Anahtar Kelimeler : High-sensitivity cardiac troponin T (hs-TnT), N-terminal proB-type natriuretic peptide (NT-proBNP), Non-acute cardiovascular events, In-hospital elderly patients
Cilt: 14
Sayfa: 81 - 90
Sınıflandırma :
3
Midkine Is a Potential Urinary Biomarker for Non-Invasive Detection of Bladder Cancer with Microscopic Hematuria
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Dergi Makalesi
Open Access
Kaynak :
Directory of Open Access Journals (DOAJ)
Dergi:
OncoTargets and Therapy
Yazar(lar) :
Lin H , Zhou Q , Wu W , Ma Y
Yıl :
2020
Wu W
Yayıncı Web Sitesi
Kaydet Alıntıla
Hao Lin,1 Qingwen Zhou,1 Weichu Wu,1 Yulin Ma1,2 1Department of Urology, The Second Affiliated Hospital of Shantou University Medical College, Shantou, Guangdong 515041, People’s Republic of China; 2Department of Urology, Hongsen Hospital of Harbin Medical University, Sanya, Hainan 572000, People’s Republic of ChinaCorrespondence: Yulin Ma Email mayulin2017@163.com Background: To determine the role of Midkine (MDK) in non-invasive detection of bladder cancer (Bca) and the relationship with Ki67.Methods: Sixty-five Bca patients and 55 non-Bca patients or healthy volunteers were enrolled and voided urine samples were prospectively obtained on the first day of enrollment. Tissue samples were collected by surgery. MDK and Ki67 expressions were analyzed by immunohistochemistry and Western Blot (WB). Specificity and sensitivity of MDK mRNA testing in the detection of Bca were determined by Receiver Operating Characteristic curve (ROC). The relationship between MDK and Ki67 was also assessed.Results: MDK was overexpressed in Bca tissues than that in the non-cancer tissues. The specificity and sensitivity for MDK mRNA testing in urine in the identification of Bca was 80% and 72.3%. MDK detected 85.7% of high-grade tumors, 87.5% of muscle-invasive tumors and 79.4% of tumors larger than 3 cm in patients without gross hematuria. Microscopic hematuria may even increase the detection rate of Bca by MDK testing. Furthermore, the correlation of MDK and Ki67 was found positive.Conclusion: MDK was overexpressed in Bca tissues and positively correlated with Ki67. MDK might be a potential biomarker for the detection of Bca, especially for those without gross hematuria but with microscopic hematuria.Keywords: Midkine, non-invasive detection, bladder cancer, biomarker, Ki67
Anahtar Kelimeler : midkine, non-invasive detection, bladder cancer, biomarker
ISSN: 1178-6930
Cilt: 12
Sayfa: 11765 - 11775
Sınıflandırma :
4
Liposomes containing glycocholate as potential oral insulin delivery systems: preparation, in vitro characterization, and improved protection against enzymatic degradation
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Dergi Makalesi
Open Access
Kaynak :
Directory of Open Access Journals (DOAJ)
Dergi:
International Journal of Nanomedicine
Yazar(lar) :
Niu M , Lu Y , Hovgaard L , Wu W
Yıl :
2011
Wu W
Yayıncı Web Sitesi
Kaydet Alıntıla
Mengmeng Niu1, Yi Lu1, Lars Hovgaard2, Wei Wu11School of Pharmacy, Fudan University, Shanghai, People's Republic of China; 2Oral Formulation Development, Novo Nordisk A/S, Maalov, DenmarkBackground: Oral delivery of insulin is challenging and must overcome the barriers of gastric and enzymatic degradation as well as low permeation across the intestinal epithelium. The present study aimed to develop a liposomal delivery system containing glycocholate as an enzyme inhibitor and permeation enhancer for oral insulin delivery.Methods: Liposomes containing sodium glycocholate were prepared by a reversed-phase evaporation method followed by homogenization. The particle size and entrapment efficiency of recombinant human insulin (rhINS)-loaded sodium glycocholate liposomes can be easily adjusted by tuning the homogenization parameters, phospholipid:sodium glycocholate ratio, insulin:phospholipid ratio, water:ether volume ratio, interior water phase pH, and the hydration buffer pH.Results: The optimal formulation showed an insulin entrapment efficiency of 30% ± 2% and a particle size of 154 ± 18 nm. A conformational study by circular dichroism spectroscopy and a bioactivity study confirmed the preserved integrity of rhINS against preparative stress. Transmission electron micrographs revealed a nearly spherical and deformed structure with discernable lamella for sodium glycocholate liposomes. Sodium glycocholate liposomes showed better protection of insulin against enzymatic degradation by pepsin, trypsin, and a-chymotrypsin than liposomes containing the bile salt counterparts of sodium taurocholate and sodium deoxycholate.Conclusion: Sodium glycocholate liposomes showed promising in vitro characteristics and have the potential to be able to deliver insulin orally.Keywords: liposomes, glycocholate, insulin, enzymatic degradation, oral
Cilt: 2011
Sayı: default
Sayfa: 1155 - 1166
Sınıflandırma :
5
Evaluation of the clinical effect of small-volume resuscitation on uncontrolled hemorrhagic shock in emergency
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Dergi Makalesi
Open Access
Kaynak :
Directory of Open Access Journals (DOAJ)
Dergi:
Therapeutics and Clinical Risk Management
Yazar(lar) :
Zhao G , Wu W , Feng QM , Sun J
Yıl :
2017
Wu W
Yayıncı Web Sitesi
Kaydet Alıntıla
Gang Zhao, Wei Wu, Qi-ming Feng, Jian Sun Department of Emergency, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, Shanghai, People’s Republic of China Objective: The objective of the present study was to explore the resuscitative effect of small-volume resuscitation on uncontrolled hemorrhagic shock in emergency. Methods: In this study, the resuscitative effects in 200 trauma patients with uncontrolled hemorrhagic shock in emergency were studied. Half of these patients were infused with hypertonic/hyperoncotic fluid (small-volume resuscitation group, n=100), whereas the rest were infused with Hespan and lactated Ringer’s solution (conventional fluid resuscitation group, n=100). The changes in hemodynamics, coagulation function, blood biochemistry, blood hematology, and the average infusion volume in both the groups were comparatively studied. Results: It was found that the hemodynamics were improved in both the groups after resuscitation. Interestingly, compared with trauma patients infused with Hespan and lactated Ringer’s solution, the growth rate, range, and time duration of the mean arterial pressure of the patients in small-volume resuscitation group increased significantly, and the shock index decreased progressively; in the 60th min after the resuscitation, blood index including hemoglobin, hematocrit, red blood cells, white blood cells, and platelet declined, whereas prothrombin time and activated partial thromboplastin time were prolonged in both the groups, but these changes were less obvious in the small-volume group. In addition, the average infusion volume of patients in the small-volume group was less than that of patients in conventional fluid resuscitation group.Conclusion: Featured with small infusion volume and less influence to coagulation function and homeostasis of human body, small-volume resuscitation possesses a significantly higher resuscitative effect. Therefore, trauma patients may have a better chance to maintain the hemodynamic stability and the survival rate, or recovery speed will be increased when traditional aggressive fluid resuscitation is replaced by small-volume resuscitation. Keywords: small-volume resuscitation, shock, hemorrhage, emergency
Anahtar Kelimeler : Small volume resuscitation, shock, hemorrhage, emergency
Cilt: 13
Sayfa: 387 - 392
Sınıflandırma :
6
The clinical significance of γ-catenin in acute myeloid leukemia
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Dergi Makalesi
Open Access
Kaynak :
Directory of Open Access Journals (DOAJ)
Dergi:
OncoTargets and Therapy
Yazar(lar) :
Xu J , Wu W , Shen W , Liu P
Yıl :
2016
Wu W
Yayıncı Web Sitesi
Kaydet Alıntıla
Jiadai Xu,1 Wei Wu,1 Wenyi Shen,1 Peng Liu2 1Department of Hematology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, 2Department of Hematology, Zhongshan Hospital, Fudan University, Shanghai, People’s Republic of China Abstract: Dysregulation of γ-catenin may function as an oncogenic factor in various malignancies. We investigated γ-catenin expression in acute myeloid leukemia (AML) and explored its role in the pathogenesis of AML. γ-Catenin was significantly overexpressed in AML patients compared to healthy donors. The γ-catenin expression in AML patients with lower white blood cells (<30×109/L) was significantly higher than those with higher white blood cells (≥30×109/L). The expression levels of γ-catenin in AML patients with mutated CEBPα were significantly higher than those with unmutated CEBPα. AML patients with lower γ-catenin levels were more likely to achieve complete remission compared with patients who have higher γ-catenin levels. In K562 cells, γ-catenin knockdown suppressed cellular proliferation, while the cellular migration was greatly enhanced. Moreover, knocking down of γ-catenin enhanced the cytotoxicity of decitabine in K562 cells. Our investigation has indicated a potential role of γ-catenin in the pathogenesis of AML. Keywords: γ-catenin, acute myeloid leukemia, AML, decitabine, prognosis, bone marrow, shRNA
Anahtar Kelimeler : γ-catenin, Acute Myeloid Leukemia (AML), Decitabine
ISSN: 1178-6930
Cilt: 2016
Sayı: 1
Sayfa: 3861 - 3871
7
Inhibition of Skp2 Sensitizes Chronic Myeloid Leukemia Cells to Imatinib
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Dergi Makalesi
Open Access
Kaynak :
Directory of Open Access Journals (DOAJ)
Dergi:
Cancer Management and Research
Yazar(lar) :
Chen X , Huang Z , Wu W , Xia R
Yıl :
2020
Wu W
Yayıncı Web Sitesi
Kaydet Alıntıla
Xiaowen Chen, Zhenqi Huang, Wei Wu, Ruixiang Xia Department of Hematology, The First Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230022, People’s Republic of ChinaCorrespondence: Ruixiang Xia Email xiaruixiang@ahmu.edu.cnIntroduction: Skp2 is an E3 ubiquitin ligase that plays an important role in modulating tumor progression. The mechanisms underlying Skp2 in the promotion of proliferation and its function in the primary resistance to tyrosine kinase inhibitors (TKIs) in human CML remain to be determined. This study aimed to investigate the function of Skp2 in CML progression as well as its effects on TKI sensitivity.Methods: Expression of Skp2 in leukocytes from patients with CML and normal blood samples was analyzed by qRT-PCR. Cell proliferation was analyzed by EdU incorporation and cell counting assays. Luciferase reporter and chromatin immunoprecipitation assays were used for examination of the effects of CREB on Skp2 expression. The apoptosis in vitro of K562 cells was analyzed by MTT and caspase 3/7 activity assays.Results: The present study demonstrates that Skp2 was expressed at a higher level in patients with CML compared with healthy donors, and the elevated expression of Skp2 is critical for CML cell proliferation. Mechanistically, Skp2 was transcriptionally upregulated by CREB responsive to the PI3K/Akt signaling pathway. Furthermore, inhibition of Skp2 expression by shRNAs or blocking the PI3K/Akt/CREB pathway greatly enhances the sensitivity of CML cells to Imatinib treatment.Conclusion: We conclude that the PI3K/Akt/CREB axis regulates the sensitivity of K562 cells to Imatinib via mediating Skp2 expression. The present study revealed an unknown role of Skp2 in CML progression and provided new aspects on the Skp2-modulated TKI sensitivity in CML, contributing to the development of potential therapeutic anticancer drugs.Keywords: Skp2, PI3K/Akt, CREB, Imatinib
Anahtar Kelimeler : pi3k/akt, imatinib
ISSN: 1179-1322
Cilt: 12
Sayfa: 4777 - 4787
Sınıflandırma :
8
01 Adsorption and wetting of protein affected by bionic surface texture desig nfor artificial organs application
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Dergi Makalesi
Kaynak :
The BMJ
Dergi:
Journal of Investigative Medicine
Yazar(lar) :
Wu W , Fan BX , Chen GM
Yıl :
2017
Wu W
Yayıncı Web Sitesi
Tam Metin
Kaydet Alıntıla
DOI : 10.1136/jim-2017-mebabstracts.1
ISSN: 1081-5589
Sayı: 7
Cilt: 65
Sayfa: A1.1-A1
Sınıflandırma :
9
Effectiveness of water-based Liuzijue exercise on respiratory muscle strength and peripheral skeletal muscle function in patients with COPD
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Dergi Makalesi
Open Access
Kaynak :
Directory of Open Access Journals (DOAJ)
Dergi:
International Journal of COPD
Yazar(lar) :
Wu W , Liu X , Liu J , Li P , Wang Z
Yıl :
2018
Wu W
Yayıncı Web Sitesi
Kaydet Alıntıla
Weibing Wu,1,* Xiaodan Liu,2,* Jingxin Liu,1 Peijun Li,1 Zhenwei Wang3 1Department of Sports Medicine, Shanghai University of Sport, 2School of Rehabilitation Medicine, Shanghai University of Traditional Chinese Medicine, 3Department of Respiratory Medicine, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai, People’s Republic of China *These authors contributed equally to this work Objects: The purpose of this study was to quantitatively assess the effects of water-based Liuzijue exercise on patients with COPD and compare it with land-based Liuzijue exercise.Materials and methods: Participants were randomly allocated to one of three groups: the water-based Liuzijue exercise group (WG), the land-based Liuzijue exercise group (LG), and the control group (CG). CG participants accepted no exercise intervention, while training groups performed Liuzijue exercise according to Health Qigong Liuzijue (People’s Republic of China) in different environments for 60-min sessions twice a week for 3 months.Results: Of the 50 patients enrolled, 45 (90%) completed the 3-month intervention. The CG showed decreased expiratory muscle strength, extensor and flexor endurance ratio (ER) of the elbow joints and flexor peak torque (PT), total work (TW), and ER of the knee joints (p<0.05). Both training groups showed improved respiratory muscle strength, which differed from the CG (p<0.001). In addition, extensor and flexor TW of the elbow joints in the training groups were increased (p<0.01), and the WG differed from the CG in extensor TW and ER and flexor TW (p<0.01), while the LG differed from the CG in flexor TW and extensor ER (p<0.05). PT, PT/body weight (BW), and TW in the knee joint extensor in the training groups were increased as well (PT and PT/BW: p<0.05, TW: p<0.01), and the WG differed from the CG in terms of knee joints outcomes, while the LG differed from the CG in flexor TW only (p<0.05).Conclusion: Water-based Liuzijue exercise has beneficial effects on COPD patients’ respiratory muscle strength and peripheral skeletal muscle function, and additional benefits may exist in endurance of upper limbs and strength and endurance of lower limbs when compared with land-based Liuzijue exercise. Keywords: COPD, Liuzijue exercise, water-based exercise, respiratory muscle strength, isokinetic muscle strength, quantitative assessment
Anahtar Kelimeler : chronic obstructive pulmonary disease, Liuzijue exercise, water-based exercise, respiratory muscle strength, isokinetic muscle strength, quantitative assessment
Cilt: 13
Sayfa: 1713 - 1726
10
Minichromosome maintenance protein 2 correlates with the malignant status and regulates proliferation and cell cycle in lung squamous cell carcinoma
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Dergi Makalesi
Open Access
Kaynak :
Directory of Open Access Journals (DOAJ)
Dergi:
OncoTargets and Therapy
Yazar(lar) :
Wu W , Wang X , Shan C , Li Y , Li F
Yıl :
2018
Wu W
Yayıncı Web Sitesi
Kaydet Alıntıla
Wei Wu,1,* Xianwei Wang,1,* Changting Shan,1 Yong Li,2 Fengzhu Li3 1Department of Respiratory Medicine, Affiliated Hospital of Jining Medical University, Jining, Shandong 272029, People’s Republic of China; 2Department of Emergency, Affiliated Hospital of Jining Medical University, Jining, Shandong 272029, People’s Republic of China; 3Department of Paediatric Surgery, Jining No 1 People’s Hospital, Jining, Shandong 272011, People’s Republic of China *These authors contributed equally to this work Background: Minichromosome maintenance protein 2 (MCM2), which is a member of MCM family, has been found to be a relevant marker for progression and prognosis in a variety of human cancers. Due to lack of effective therapeutic target in lung squamous cell carcinoma (LUSC) patients, the aim of our study was to screen and identify biomarkers which are associated to clinicopathological characteristics including prognosis in LUSC patients. Methods: The expression status of MCM2 in lung cancer was analyzed using the publicly available Gene Expression Omnibus databases (GSE3268 and GSE10245). The mRNA and protein expression of MCM2 in lung cancer tissues and cell lines was detected by quantitative real-time PCR and Western blot, and the association between MCM2 expression and clinicopathological factors was analyzed by immunohistochemistry. The loss-of-function study of MCM2 was conducted in LUSC cell lines. Results: In our study, we found MCM2 expression was increased in LUSC tissues compared with paired adjacent normal lung tissues or lung adenocarcinoma tissues through analyzing microarray data sets (GSE3268 and GSE10245), which confirmed that MCM2 mRNA and protein were overexpressed in LUSC tissues and cell lines. Meanwhile, we analyzed the association between MCM2 protein expression and clinicopathological characteristics of LUSC patients, and found high expression of MCM2 protein was obviously associated with malign differentiated degree, advanced clinical stage, large tumor size, more lymph node metastasis and present distant metastasis. The survival analysis showed MCM2 overexpression was an independent unfavorable prognostic factor for LUSC patients. Conclusion: MCM2 is involved in the development and progression of LUSC as an oncogene, and thereby may act as a potential therapeutic target for LUSC patients. Keywords: MCM2, lung cancer, lung squamous cell carcinoma, biomarker, proliferation, cell cycle
Anahtar Kelimeler : lung cancer, lung squamous cell carcinoma, biomarker, proliferation, cell-cycle
ISSN: 1178-6930
Cilt: 11
Sayfa: 5025 - 5034
Sınıflandırma :
11
Effects of marital status on survival of hepatocellular carcinoma by race/ethnicity and gender
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Dergi Makalesi
Open Access
Kaynak :
Directory of Open Access Journals (DOAJ)
Dergi:
Cancer Management and Research
Yazar(lar) :
Wu W , Fang D , Shi D , Bian X , Li L
Yıl :
2018
Wu W
Yayıncı Web Sitesi
Kaydet Alıntıla
Wenrui Wu,1,2 Daiqiong Fang,1,2 Ding Shi,1,2 Xiaoyuan Bian,1,2 Lanjuan Li1,2 1State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, The First Affiliated Hospital, School of Medicine, Zhejiang University, 2Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, Hangzhou, People’s Republic of China Purpose: It is well demonstrated that being married is associated with a better prognosis in multiple types of cancer. However, whether the protective effect of marital status varied across race/ethnicity and gender in patients with hepatocellular carcinoma remains unclear. Therefore, we aimed to evaluate the roles of race/ethnicity and gender in this relationship.Patients and methods: We identified eligible patients from Surveillance, Epidemiology and End Results (SEER) database during 2004–2012. Overall and cancer-specific survival differences across marital status were compared by Kaplan–Meier curves. We also estimated crude hazard ratios (CHRs) and adjusted hazard ratios (AHRs) with 95% confidence intervals (CIs) for marital status associated with survival by race/ethnicity and gender in Cox proportional hazard models.Results: A total of 12,168 eligible patients diagnosed with hepatocellular carcinoma were included. We observed that married status was an independent protective prognostic factor for overall and cancer-specific survival. In stratified analyses by race/ethnicity, the AHR of overall mortality (unmarried vs married) was highest for Hispanic (AHR =1.25, 95% CI, 1.13–1.39; P<0.001) and lowest for Asian or Pacific Islander (AHR =1.13; 95% CI, 1.00–1.28; P=0.042). Stratified by gender, the AHR was higher in males (AHR =1.27; 95% CI, 1.20–1.33; P<0.001).Conclusion: We demonstrated that married patients obtained better survival advantages. Race/ethnicity and gender could influence the magnitude of associations between marital status and risk of mortality. Keywords: primary hepatocellular carcinoma, SEER, being married, race, gender, prognosis
Anahtar Kelimeler : Primary hepatocellular carcinoma, being married, Gender, Prognosis.
ISSN: 1179-1322
Cilt: 10
Sayfa: 23 - 32
Sınıflandırma :
12
Relationship between high-sensitivity cardiac troponin T and the prognosis of elderly inpatients with non-acute coronary syndromes
Dergi Makalesi

Dergi Makalesi
Open Access
Kaynak :
Directory of Open Access Journals (DOAJ)
Dergi:
Clinical Interventions in Aging
Yazar(lar) :
Wu W , Li DX , Wang Q , Xu Y , Cui YJ
Yıl :
2018
Wu W
Yayıncı Web Sitesi
Kaydet Alıntıla
Wei Wu,1 Dong-Xia Li,1 Qing Wang,2 Ying Xu,2 Yun-Jing Cui2 1General Practice Wards, Fuxing Hospital Affiliated to Capital Medical University, Beijing, People’s Republic of China; 2Department of Geriatrics, Fuxing Hospital Affiliated to Capital Medical University, Beijing, People’s Republic of China Objective: Low-level high-sensitivity cardiac troponin T (hs-cTnT) increases in elderly population. In this study, the relationship between hs-cTnT level and all-cause death of elderly inpatients with non-acute coronary syndrome (non-ACS) after discharge from the hospital was investigated. Materials and methods: Non-ACS patients aged >65 years admitted in the General Practice Wards and Department of Geriatrics of Fuxing Hospital Affiliated to Capital Medical University were enrolled in the study. The patients were grouped according to the tertiles of hs-cTnT levels. Biochemical markers, hs-cTnT, and amino-terminal pro-brain natriuretic peptide were measured. The median follow-up period was 47 months, and all-cause deaths of the patients were observed. Results: A total of 722 patients, including 473 males and 249 females, aged 65–98 (82.43±5.98) years were enrolled in the study. The level of hs-cTnT was found to be higher in males, and increased with age and comorbidities (P<0.01). Compared with low-level group, NT-proBNP level of patients in high-level group was higher, while hemoglobin (Hb), estimated glomerular filtration rate (eGFR), and left ventricular ejection fraction (LVEF) levels were lower (P<0.001). The mortality rate increased significantly with increased hs-cTnT levels (P<0.001). The total number of deaths was 136 (18.8%), and of these, 108 (79.4%) were noncardiac deaths. Risk of all-cause deaths in the highest hs-cTnT level group was 7.3 times higher than that of the lowest hs-cTnT level group (95% CI: 4.29–12.51, P<0.001). After adjusting for gender, age, comorbidities, NT-proBNP, Hb, eGFR, and LVEF, hs-cTnT level still affected the patient’s survival time (HR: 3.01, 95% CI: 1.67–5.43, P<0.001). Conclusion: These findings suggest that low-level hs-cTnT was increased in elderly inpatients without ACS. They further highlight that baseline hs-cTnT level was associated with increased risk of all-cause deaths among patients after their discharge, and most deaths were from non-cardiovascular diseases. Keywords: high-sensitivity cardiac troponin T, elderly inpatients, non-acute coronary syndrome, prognosis
Anahtar Kelimeler : High-sensitivity cardiac troponin T, Elderly inpatients, Non-acute coronary syndrome, Prognosis
Cilt: 13
Sayfa: 1091 - 1098
13
Prognostic Significance of Rab27A and Rab27B Expression in Esophageal Squamous Cell Cancer
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Dergi Makalesi
Open Access
Kaynak :
Directory of Open Access Journals (DOAJ)
Dergi:
Cancer Management and Research
Yazar(lar) :
Yu F , Wu W , Liang M , Huang Y , Chen C
Yıl :
2020
Wu W
Yayıncı Web Sitesi
Kaydet Alıntıla
Fengqiang Yu,* Weihan Wu,* Mingqiang Liang,* Yu Huang, Chun Chen Department of Thoracic Surgery, Fujian Medical University Union Hospital, Fuzhou, Fujian, People’s Republic of China*These authors contributed equally to this workCorrespondence: Chun ChenDepartment of Thoracic Surgery, Fujian Medical University Union Hospital, #29 Xinquan Road, Fuzhou, Fujian 350001, People’s Republic of ChinaTel +86 13365910325Fax +86 591-83357896-8408Email chenchun0209@163.comPurpose: Rab27A and Rab27B, members of the Rab family of small GTPases, have aberrant expression and exert different roles in various cancers. However, their expression and potential prognostic values in esophageal squamous cell cancer (ESCC) still remain elusive. In the present study, we explored the association of Rab27A and Rab27B expression with clinical significance and prognosis in ESCC.Patients and Methods: A total of 100 surgically resected ESCC tissues were examined to evaluate Rab27A and Rab27B expression levels using the immunohistochemistry method. The relationship of Rab27A and Rab27B with clinicopathological features and prognosis was analyzed. We also investigated the correlation between Rab27A and Rab27B through external and internal validation.Results: High-expression Rab27A was found to be significantly correlated with N (p=0.045) and TNM (p=0.005) stage, while up-regulated Rab27B was remarkably associated with N stage (p=0.033), TNM stage (p=0.009), and differentiation (p=0.013). High expression of both Rab27A and Rab27B had a worse overall survival (OS) rate. In addition, multivariate Cox regression analyses were utilized to validate that Rab27B expression is an independent prognostic factor for unfavorable OS. Further combined analyses showed that the Rab27Alow/Blow group had a superior OS rate than the Rab27Ahigh/Blow group, Rab27Alow/Bhigh group, and Rab27Ahigh/Bhigh group. Nevertheless, the latter three groups displayed rare significance between each two comparisons. Furthermore, our data demonstrated that Rab27A expression was positively correlated with Rab27B expression, which were also verified in TCGA datasets.Conclusion: Rab27A and Rab27B expression levels could be potentially novel prognostic biomarkers in ESCC.Keywords: Rab27A, Rab27B, esophageal squamous cell cancer, prognosis, survival analysis
Anahtar Kelimeler : rab27a, rab27b, esophageal squamous cell cancer, prognosis, survival analysis
ISSN: 1179-1322
Cilt: 12
Sayfa: 6353 - 6361
Sınıflandırma :
14
lncRNA GAS5/miR-452-5p Reduces Oxidative Stress and Pyroptosis of High-Glucose-Stimulated Renal Tubular Cells
Dergi Makalesi

Dergi Makalesi
Open Access
Kaynak :
Directory of Open Access Journals (DOAJ)
Dergi:
Diabetes, Metabolic Syndrome and Obesity : Targets and Therapy
Yazar(lar) :
Xie C , Wu W , Tang A , Luo N , Tan Y
Yıl :
2019
Wu W
Yayıncı Web Sitesi
Kaydet Alıntıla
Cuisong Xie,1 Weiling Wu,1 Ainan Tang,2 Ning Luo,1 Yanfei Tan1 1Department of Endocrinology, Chenzhou No.1 People’s Hospital, Chenzhou, Hunan 423000, People’s Republic of China; 2Department of Endocrinology, Chenzhou 3rd People’s Hospital, Chenzhou, Hunan 423000, People’s Republic of ChinaCorrespondence: Cuisong XieDepartment of Endocrinology, Chenzhou No.1 People’s Hospital, 102 Xijie Street, Beihu District, Chenzhou, Hunan 423000, People’s Republic of ChinaEmail xcszgr@163.comBackground: Diabetic nephropathy (DN) is the leading cause of end-stage renal failure worldwide. lncRNAs are demonstrated to improve the DN by changing the expression of miRNAs. This study was aimed to investigate the effect of lncRNA GAS5/miR-452-5p on the inflammation, oxidative stress and pyroptosis of high-glucose-induced renal tubular cells.Methods: HK-2 cells were induced by HG to simulate DN cells. RT-qPCR analysis confirmed the transfection effects and detected the expression of GAS5, NLRP3, caspase1, IL-1β, pro-caspase1, pro-IL-1β, GSDMD-N and miR-452-5p. Western blot analysis determined the protein expression of NLRP3, caspase1, IL-1β, pro-caspase1, pro-IL-1β and GSDMD-N. The expression of GSDMD-N was also verified by immunofluorescence. The levels of TNF-α, IL-6, MCP-1, ROS, MDA and SOD were measured by commercial assay kits, respectively. Dual-luciferase reporter assay indicated that GAS5 could combine with miR-452-5p.Results: GAS5 expression was decreased in HG-induced HK-2 cells. GAS5 overexpression could decrease the levels of TNF-α, IL-6, MCP-1, ROS and MDA and increase the levels of SOD. Moreover, GAS5 overexpression suppressed the expression of NLRP3, caspase1, IL-1β and GSDMD-N, and the results of immunofluorescence verified the above results. miR-452-5p interference could cause the same changes as GAS5 overexpression for HG-induced HK-2 cells, and GAS5 inhibition could reverse the effect of miR-452-5p interference.Conclusion: GAS5 overexpression inhibited the inflammation, oxidative stress and pyroptosis of HG-induced renal tubular cells by downregulating the expression of miR-452-5p.Keywords: lncRNA GAS5, miR-452-5p, oxidative stress, pyroptosis, high glucose, renal tubular cells
Anahtar Kelimeler : lncrna gas5, mir-452-5p, oxidative stress, pyroptosis, high glucose, renal tubular cells
ISSN: 1178-7007
Cilt: 12
Sayfa: 2609 - 2617
Sınıflandırma :
15
Osteoblast/bone-tissue responses to porous surface of polyetheretherketone–nanoporous lithium-doped magnesium silicate blends’ integration with polyetheretherketone
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Open Access
Kaynak :
Directory of Open Access Journals (DOAJ)
Dergi:
International Journal of Nanomedicine
Yazar(lar) :
Wang L , Zhang K , Hao Y , Liu M , Wu W
Yıl :
2019
Wu W
Yayıncı Web Sitesi
Kaydet Alıntıla
Lei Wang, Kai Zhang, Yongqiang Hao, Ming Liu, Wen WuDepartment of Orthopaedics, Shanghai Key Laboratory of Orthopaedic Implants, Shanghai Ninth People’s Hospital, Shanghai Jiaotong University School of Medicine, Shanghai 200011, People’s Republic of ChinaAbstract: The porous surface of a polyetheretherketone (PK)–nanoporous lithium-doped magnesium silicate (NLS) blend (PKNLS) was fabricated on a PK surface by layer-by-layer pressuring, sintering, and salt-leaching. As controls, porous surfaces of a PK/lithium-doped magnesium silicate blend (PKLS) and PK were fabricated using the same method. The results revealed that porosity, water absorption, and protein absorption of the porous surface of PKNLS containing macropores and nanopores were obviously enhanced compared to PKLS and PK containing macropores without nanopores. In addition, PKNLS, with both macroporostiy and nanoporosity, displayed the highest ability of apatite mineralization in simulated body liquid, indicating excellent bioactivity. In vitro responses (including adhesion, proliferation, and differentiation) of MC3T3E1 cells to PKNLS were significantly enhanced compared to PKLS and PK. In vivo implantation results showed that new bone grew into the macroporous surface of PKNLS, and the amount of new bone for PKNLS was the highest. In short, PKNLS integration with PK significantly promoted cells/bone-tissue responses and exhibited excellent osteogenesis in vivo, which might have great potential for bone repair.Keywords: polyetheretherketone, nanoporous lithium doped magnesium silicate, porously bioactive surface, cells responses, osteogenesis
Anahtar Kelimeler : polyetheretherketone, nanoporous lithium doped magnesium silicate, porously bioactive surface, cells responses, osteogenesis
Cilt: 14
Sayfa: 4975 - 4989
Sınıflandırma :
16
Microporous elastomeric membranes fabricated with polyglycerol sebacate improved guided bone regeneration in a rabbit model
Dergi Makalesi

Dergi Makalesi
Open Access
Kaynak :
Directory of Open Access Journals (DOAJ)
Dergi:
International Journal of Nanomedicine
Yazar(lar) :
Jian B , Wu W , Song Y , Tan N , Ma C
Yıl :
2019
Wu W
Yayıncı Web Sitesi
Kaydet Alıntıla
Bo Jian,1,2 Wei Wu,2 Yingliang Song,1 Naiwen Tan,3 Chao Ma1 1State Key Laboratory of Military Stomatology, Department of Implant Dentistry, School of Stomatology, The Fourth Military Medical University, Xi’an, Shaanxi, 710032, China; 2Department of Oral & Maxillofacial Surgery, School of Stomatology, The Fourth Military Medical University, Xi’an, Shaanxi, China; 3Department of Stomatology, Hospital 463 of PLA, Shenyang, Liaoning, People’s Republic of China Purpose: We aimed to fabricate guided bone regeneration (GBR) membrane using polyglycerol sebacate (PGS) and investigate the impact of scaffold pore size on osteogenesis.Materials and methods: PGS microporous membrane was fabricated by salt-leaching technique with various pore sizes. Twenty-eight male New Zealand rabbits were randomly divided into four groups: 25 µm PGS membrane, 53 µm PGS membrane, collagen membrane, and blank control group. Subsequently, standardized and critical-sized tibia defects were made in rabbits and the defective regions were covered with the specifically prepared membranes. After 4 and 12 weeks of in vivo incubation, bone samples were harvested from tibia. Micro-computed tomography scanning was performed on all bone samples. A three-dimensional visible representation of the constructs was obtained and used to compare the ratios of the ossifying volume to total construct volume (bone volume to tissue volume [BV/TV]) of each sample in different groups; then, bone samples were stained with H&E and Masson’s trichrome stain for general histology.Results: At 4 weeks, the BV/TV in the 25 µm PGS group was found higher than that in the 53 µm PGS and collagen groups. At 12 weeks, the bone defect site guided by the 25 µm PGS membrane was almost completely covered by the new bone. However, the site guided by the 53 µm PGS membrane or collagen membrane was covered only most of the defects and the left part of the defect was unoccupied. Histological observation further verified these findings.Conclusion: We thus concluded that the 25 µm PGS membrane played an advantageous role during 4–12 weeks as compared with those earlier degraded counterparts. Keywords: bone defect, biological membrane, degradation, salt-leaching technique, tibia
Anahtar Kelimeler : bone defect, biological membrane, degradation, salt-leaching technique, tibia
Cilt: 14
Sayfa: 2683 - 2692
17
Anticancer potential of Salvia miltiorrhiza and its tanshinones: an efficacy perspective
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Open Access
Kaynak :
Directory of Open Access Journals (DOAJ)
Dergi:
Botanics : Targets and Therapy
Yazar(lar) :
Wu W , Zhang Y , Tang SN , Ye M , Lü J , Jiang C
Yıl :
2016
Wu W
Yayıncı Web Sitesi
Kaydet Alıntıla
Wei Wu,1 Yong Zhang,1 Suni Tang,1 Min Ye,2 Junxuan Lü,1 Cheng Jiang1 1Department of Biomedical Sciences, Texas Tech University Health Sciences Center School of Pharmacy, Amarillo, TX, USA; 2State Key Laboratory of Natural and Biomimetic Drugs, School of Pharmaceutical Sciences, Peking University, Beijing, People’s Republic of China Abstract: Salvia miltiorrhiza Bunge (Danshen in Chinese or Tanshen in Anglicized literature) is a well-known Traditional Chinese Medicine herbal remedy for treating cardiovascular- and cerebrovascular-related disorders. To date, >40 hydrophorbic tanshinones and structurally related compounds have been isolated from the Danshen root, as have some 50 hydrophilic phenolics and other minor components. In the past 2 decades, a large quantity of literature has reported inhibitory activities of tanshinones against cancers of various organ sites in cell culture models, and in some cases with efficacy confirmation in preclinical animal cancer models. This study follows up on a 2012 review we published on the sources, pharmacokinetics, and anticancer activities of tanshinones. Here, we update on the recent progress in understanding the anticancer potential of tanshinones and derivatives and critically assess merits of these entities for future research and development. Overall, potency data from in vivo efficacy assessment experiments in preclinical models varied from nil for chemoprevention of a prostate carcinogenesis model to strong inhibition of some xenograft or allograft models. Lack of uniformity of excipients, doses, and routes of administration aside, we caution that the reviewed data should be appreciated in balance of publication bias exemplified by our own data from primary carcinogenesis study and false positivity. Novel formulations and chemical modifications had been made to improve the poor solubility and bioavailability of tanshinones. Human clinical studies so far dealt with case reports of tanshinone IIA use and small-scale trials on Danshen-containing formulas with chemotherapy for cancers of multiple organ sites in People’s Republic of China. Available human data are not sufficient for supporting any anticancer indication of tanshinones. Keywords: human studies, cancer therapy, cancer chemoprevention, preclinical efficacy
Anahtar Kelimeler : tanshinones, pharmacokinetics, cancer therapy, cancer chemoprevention, preclinical efficacy, cellular and molecular targets
ISSN: 1179-9897
Cilt: 2016
Sayı: 1
Sayfa: 45 - 58
Sınıflandırma :
18
Risk Stratification Based on Metastatic Pelvic Lymph Node Status in Stage IIIC1p Cervical Cancer
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Dergi Makalesi
Open Access
Kaynak :
Directory of Open Access Journals (DOAJ)
Dergi:
Cancer Management and Research
Yazar(lar) :
Li A , Wang L , Jiang Q , Wu W , Huang B , Zhu H
Yıl :
2020
Wu W
Yayıncı Web Sitesi
Kaydet Alıntıla
Anyang Li,1,2 Luhui Wang,2 Qi Jiang,2 Wenlie Wu,2 Baoyou Huang,2 Haiyan Zhu1,2 1Department of Gynecology, Shanghai First Maternity and Infant Hospital, Tongji University School of Medicine, Shanghai 200126, People’s Republic of China; 2Department of Gynecology, The First Affiliated Hospital of Wenzhou Medical University, Wenzhou 325027, People’s Republic of ChinaCorrespondence: Haiyan ZhuDepartment of Gynecology, Shanghai First Maternity and Infant Hospital, Tongji University School of Medicine, Shanghai 200126, People’s Republic of ChinaTel +86 57755069162Email zhuhaiyandoc@sina.comPurpose: Stage IIIC1 cervical cancer showed heterogeneous in oncologic outcomes with highly variable survival rates. Our objective was to determine the prognostic significance of removed and metastatic pelvic lymph node status and further perform risk stratification in patients with stage IIIC1p cervical cancer.Patients and Methods: Patients with stage IIIC1p cervical cancer and undergoing radical hysterectomy with lymphadenectomy in 2008– 2018 were retrospectively analyzed. Patients’ stage was classified using the revised 2018 International Federation of Gynecology and Obstetrics (FIGO) staging schema. Univariate and multivariable models were used to examine the association between removed and metastatic lymph node status and recurrence-free survival/overall survival.Results: During a median follow-up of 34 months, 73 relapses and 44 deaths were observed among 273 patients with stage IIIC1p cervical cancer. Parametrial involvement and metastatic lymph node ratio (mLNR) were identified as independent predictors for recurrence-free survival. Parametrial involvement and mLNR were independent predictors for overall survival. A stratification system was then created based on parametrial involvement and mLNR. A total of 123 (45.1%), 127 (46.5%) and 23 (8.4%) patients were classified into the low-risk, intermediate-risk, and high-risk groups, with as a 5-year recurrence-free survival of 81.7%, 51.1%, 38%, respectively. Compared to the low-risk group, the intermediate- and high-risk groups had a significantly greater risk of recurrence and death.Conclusion: The prognosis of stage IIIC1p patients varied significantly. A risk stratification system based on parametrial involvement and mLNR successfully separated patients into low, intermediate, and high-risk group. Our findings could facilitate the practical use of further stratification in Stage IIIC1p cervical cancer.Keywords: cervical cancer, stage IIIC1p, pelvic lymph node, prognosis
Anahtar Kelimeler : cervical cancer, stage iiic1p, pelvic lymph node, prognosis;
ISSN: 1179-1322
Cilt: 12
Sayfa: 6431 - 6439
Sınıflandırma :
19
Formulating food protein-stabilized indomethacin nanosuspensions into pellets by fluid-bed coating technology: physical characterization, redispersibility, and dissolution
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Dergi Makalesi
Open Access
Kaynak :
Directory of Open Access Journals (DOAJ)
Dergi:
International Journal of Nanomedicine
Yazar(lar) :
He W , Lu Y , Qi J , Chen L , Yin L , Wu W
Yıl :
2013
Wu W
Yayıncı Web Sitesi
Kaydet Alıntıla
Wei He,1,2 Yi Lu,1 Jianping Qi,1 Lingyun Chen,3 Lifang Yin,2 Wei Wu1 1School of Pharmacy, Fudan University, Key Laboratory of Smart Drug Delivery of Ministry of Education and PLA, Shanghai, 2Department of Pharmaceutics, School of Pharmacy, China Pharmaceutical University, Nanjing, Jiangsu, People's Republic of China; 3Department of Agricultural, Food and Nutritional Sciences, University of Alberta, Edmonton, AB, Canada Background: Drug nanosuspensions are very promising for enhancing the dissolution and bioavailability of drugs that are poorly soluble in water. However, the poor stability of nanosuspensions, reflected in particle growth, aggregation/agglomeration, and change in crystallinity state greatly limits their applications. Solidification of nanosuspensions is an ideal strategy for addressing this problem. Hence, the present work aimed to convert drug nanosuspensions into pellets using fluid-bed coating technology. Methods: Indomethacin nanosuspensions were prepared by the precipitation-ultrasonication method using food proteins (soybean protein isolate, whey protein isolate, ß-lactoglobulin) as stabilizers. Dried nanosuspensions were prepared by coating the nanosuspensions onto pellets. The redispersibility, drug dissolution, solid-state forms, and morphology of the dried nanosuspensions were evaluated. Results: The mean particle size for the nanosuspensions stabilized using soybean protein isolate, whey protein isolate, and β-lactoglobulin was 588 nm, 320 nm, and 243 nm, respectively. The nanosuspensions could be successfully layered onto pellets with high coating efficiency. Both the dried nanosuspensions and nanosuspensions in their original amorphous state and not influenced by the fluid-bed coating drying process could be redispersed in water, maintaining their original particle size and size distribution. Both the dried nanosuspensions and the original drug nanosuspensions showed similar dissolution profiles, which were both much faster than that of the raw crystals. Conclusion: Fluid-bed coating technology has potential for use in the solidification of drug nanosuspensions. Keywords: nanocrystals, nanosuspensions, food proteins, poorly water-soluble drugs, indomethacin, fluid-bed coating
Cilt: 2013
Sayı: default
Sayfa: 3119 - 3128
Sınıflandırma :
20
Influencing Factors and Exercise Intervention of Cognitive Impairment in Elderly Patients with Chronic Obstructive Pulmonary Disease
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Dergi Makalesi
Open Access
Kaynak :
Directory of Open Access Journals (DOAJ)
Dergi:
Clinical Interventions in Aging
Yazar(lar) :
Wang T , Mao L , Wang J , Li P , Liu X , Wu W
Yıl :
2020
Wu W
Yayıncı Web Sitesi
Kaydet Alıntıla
Ting Wang,1 Lijuan Mao,1 Jihong Wang,1 Peijun Li,1 Xiaodan Liu,2,3 Weibing Wu1 1Department of Sports Medicine, Shanghai University of Sport, Shanghai 200438, People’s Republic of China; 2School of Rehabilitation Science, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, People’s Republic of China; 3Institute of Rehabilitation Medicine, Shanghai Academy of Traditional Chinese Medicine, Shanghai 201203, People’s Republic of ChinaCorrespondence: Weibing WuDepartment of Sports Medicine, Shanghai University of Sport, Shanghai 200438, People’s Republic of ChinaTel +86 21 5125 3319Fax +86 21 5125 3380Email wwb75@126.comXiaodan LiuSchool of Rehabilitation Science, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, People’s Republic of ChinaTel +86 15800668700Email hzhp403@126.comAbstract: Chronic obstructive pulmonary disease (COPD) is a common respiratory condition characterized by airflow limitation in the elderly. Airflow limitation is partially reversible and progressive. COPD not only causes a gradual decline in lung function but also affects the function of other systems throughout the body; it also has adverse effects on the central nervous system that can lead to cognitive impairment, especially in elderly patients. Therefore, understanding the influencing factors of cognitive impairment in elderly patients with COPD and applying early intervention are crucial in improving the quality of life of patients and reducing the burden on their families and society. This article mainly discusses the related factors of cognitive impairment in elderly patients with COPD and expands the possible mechanism of exercise in improving cognitive impairment in patients with COPD to provide a reference for the clinical prevention and treatment of cognitive impairment in elderly patients with COPD.Keywords: chronic obstructive pulmonary disease, elderly people, cognitive function, influencing factors, exercise training
Anahtar Kelimeler : chronic obstructive pulmonary disease, elderly people, cognitive function, influencing factors, exercise training
Cilt: 15
Sayfa: 557 - 566
Sınıflandırma :
21
Effects of long-term home-based Liuzijue exercise combined with clinical guidance in elderly patients with chronic obstructive pulmonary disease
Dergi Makalesi

Dergi Makalesi
Open Access
Kaynak :
Directory of Open Access Journals (DOAJ)
Dergi:
Clinical Interventions in Aging
Yazar(lar) :
Li P , Liu J , Lu Y , Liu X , Wang Z , Wu W
Yıl :
2018
Wu W
Yayıncı Web Sitesi
Kaydet Alıntıla
Peijun Li,1,* Jingxin Liu,1,* Yufan Lu,1 Xiaodan Liu,2 Zhenwei Wang,3 Weibing Wu1 1Department of Sports Medicine, Shanghai University of Sport, Shanghai, People’s Republic of China; 2School of Rehabilitation Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai, People’s Republic of China; 3Department of Respiratory Medicine, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai, People’s Republic of China *These authors contributed equally to this work Purpose: This study was designed to investigate the effects of long-term home-based Liuzijue exercise combined with clinical guidance in elderly patients with chronic obstructive pulmonary disease (COPD). Methods: Forty patients with COPD at stages II–III of the Global Initiative for Chronic Obstructive Lung Disease were enrolled. The subjects were randomly allocated to the Liuzijue exercise group (LG) or control group (CG) in a 1:1 ratio. Participants in the LG performed six Liuzijue training sessions, including 4 days at home and 2 days in the hospital with clinical guidance for 60 minutes/day for 6 months. Participants in the CG conducted no exercise intervention. In addition, lung function test, 6-minute walking test (6MWT), 30-second sit-to-stand test (30 s SST), and the St George’s Respiratory Questionnaire (SGRQ) were conducted at the baseline and at the end of the intervention. Results: Thirty-six patients completed the study. The patients’ lung function improved significantly (p < 0.05) in the LG as well as the 6MWT, 30 s SST, and SGRQ score (p < 0.01). While the SGRQ total score, activity, and impact scores increased significantly (p < 0.05) in the CG. In addition, there were significant differences between the groups (p < 0.01) in regard to the values of forced expiratory volume in 1 second as a percentage of the predicted volume, 6MWT, 30 s SST, and SGRQ. Conclusions: Long-term home-based Liuzijue exercise combined with clinical guidance can effectively improve the pulmonary function, exercise capacity, and quality of life of elderly patients with moderate to severe COPD. Keywords: chronic obstructive pulmonary disease, elderly patients, Liuzijue exercise, pulmonary function, exercise capacity, quality of life
Anahtar Kelimeler : chronic obstructive pulmonary disease, elderly patients, Liuzijue exercise, pulmonary function, exercise capacity, quality of life
Cilt: 13
Sayfa: 1391 - 1399
Sınıflandırma :
22
Effects of Tai Chi on exercise capacity and health-related quality of life in patients with chronic obstructive pulmonary disease: a systematic review and meta-analysis
Dergi Makalesi

Dergi Makalesi
Open Access
Kaynak :
Directory of Open Access Journals (DOAJ)
Dergi:
International Journal of COPD
Yazar(lar) :
Wu W , Liu X , Wang L , Wang Z , Hu J , Yan J
Yıl :
2014
Wu W
Yayıncı Web Sitesi
Kaydet Alıntıla
Weibing Wu,1 Xiaodan Liu,2 Longbing Wang,1 Zhenwei Wang,3 Jun Hu,2 Juntao Yan41Department of Sports Medicine, Shanghai University of Sport, 2School of Rehabilitation Medicine, Shanghai University of Traditional Chinese Medicine, 3Department of Respiratory Medicine, 4Department of Rehabilitation Medicine, Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai, People's Republic of ChinaBackground: Tai Chi is a traditional Chinese mind-body exercise that has been widely practiced in the People's Republic of China for many centuries. This exercise has also been applied as a training modality in pulmonary rehabilitation programs for stable chronic obstructive pulmonary disease (COPD). This systematic review and meta-analysis aimed to assess the effects of Tai Chi on exercise capacity and health-related quality of life (HRQoL) in COPD patients.Methods: Electronic databases (PubMed, Embase, Web of Science, The Cochrane Library, Cumulative Index to Nursing and Allied Health Literature, ClinicalTrials.gov, China National Knowledge Infrastructure, and China Biology Medicine disc) were searched. Entries published from January 1980 to March 2014 were included in the search. Eligible studies included those that involved randomized controlled trials and those that lasted for at least 12 weeks. The primary outcome measures were six-minute walking distance (6MWD), St George's Respiratory Questionnaire (SGRQ), and Chronic Respiratory Disease Questionnaire (CRQ). Effect estimates were pooled with random-effects meta-analysis.Results: Eleven articles involving 824 patients met the inclusion criteria. All included articles compared COPD patients in a Tai Chi group versus COPD patients in nonexercise and/or physical exercise groups. The meta-analysis showed that compared with the nonexercise group, the COPD patients practicing Tai Chi demonstrated significantly enhanced 6MWD (mean difference 35.99, 95% confidence interval [CI] 15.63–56.35, P=0.0005), decreased SGRQ total score (mean difference -10.02, 95% CI -17.59, -2.45, P=0.009), and increased CRQ total score (mean difference 0.95, 95% CI 0.22–1.67, P=0.01). Compared with the physical exercise group, the Tai Chi group showed significantly reduced SGRQ total score (mean difference -3.52, 95% CI -6.07, -0.97, P=0.007), but no statistical significance was found for 6MWD between the two groups (mean difference 13.65, 95% CI -1.06, 28.37, P=0.07) in COPD patients.Conclusion: Preliminary evidence suggests that Tai Chi has beneficial effects on exercise capacity and HRQoL in COPD patients. This exercise can be recommended as an effective alternative training modality in pulmonary rehabilitation programs. Further studies are required to support the preliminary evidence and to observe the long-term effects of Tai Chi.Keywords: traditional Chinese exercise, chronic obstructive pulmonary disease, six-minute walking distance, quality of life
Cilt: 2014
Sayı: default
Sayfa: 1253 - 1263
Sınıflandırma :
23
Preparation, Characterization, and in vivo Evaluation of NK4-Conjugated Hydroxycamptothecin-Loaded Liposomes
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Dergi Makalesi
Open Access
Kaynak :
Directory of Open Access Journals (DOAJ)
Dergi:
International Journal of Nanomedicine
Yazar(lar) :
Zhou T , Zhang W , Cheng D , Tang X , Feng J , Wu W
Yıl :
2020
Wu W
Yayıncı Web Sitesi
Kaydet Alıntıla
Ting Zhou,1,* Wei Zhang,1,* Dongliang Cheng,1 Xin Tang,2 Jianfang Feng,3 Wei Wu1 1School of Pharmacy, Guilin Medical University, Guilin 541004, People’s Republic of China; 2School of Public Health, Guilin Medical University, Guilin 541004, People’s Republic of China; 3School of Pharmacy, Guangxi University of Chinese Medicine, Nanning 530200, People’s Republic of China*These authors contributed equally to this workCorrespondence: Wei WuSchool of Pharmacy, Guilin Medical University, 109 North 2nd Huancheng Road, Guilin 541004, People’s Republic of ChinaEmail wuwei@glmc.edu.cnJianfang FengSchool of Pharmacy, Guangxi University of Chinese Medicine, 13 Wuhe Avenue, Nanning 530200, People’s Republic of ChinaEmail fengjianfang@vip.163.comPurpose: In this study, NK4-conjugated hydroxycamptothecin liposomes (NK4-HCPT-Lips) were prepared with the aim of improving drug targeting to the liver.Methods: NK4-HCPT-Lips were prepared using the thin-film dispersion method. In vitro antitumor activities were evaluated by MTT assay. HCPT levels in plasma and tissues were determined via high-performance liquid chromatography (HPLC) with camptothecin as the internal standard, and the characteristics, pharmacokinetics, and bio-distribution of NK4-HCPT-Lips were evaluated.Results: The liposomes showed a regular spherical-shaped morphology, and the entrapment efficiency and drug loading capacity reached 82.5 ± 2.4% and 3.01 ± 0.23%, respectively, with a particle size of 155.6 ± 2.6 nm and a zeta potential of − 24.8 ± 3.3 mV. Inhibition effect experiments found that NK4-HCPT-Lips had a good inhibition on the HepG2 cells. Pharmacokinetic studies revealed an increase in the area under the curve and mean residence time as well as a decrease in plasma clearance (p < 0.05) of the NK4-HCPT-Lips compared to those of HCPT liposomes and a commercial HCPT injection. Tissue distribution studies showed that NK4-HCPT-Lips were present at high levels in the liver but were cleared from the kidneys.Conclusion: These results demonstrate that NK4-HCPT-Lips possess excellent liver-targeting attributes, which could enhance the therapeutic effects of drug treatments for hepatic diseases.Keywords: hydroxycamptothecin, NK4, liposomes, pharmacokinetics, bio-distribution
Anahtar Kelimeler : hydroxycamptothecin, liposomes, pharmacokinetics, bio-distribution
Cilt: 15
Sayfa: 2277 - 2286
Sınıflandırma :
24
Liposomes containing bile salts as novel ocular delivery systems for tacrolimus (FK506): in vitro characterization and improved corneal permeation
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Dergi Makalesi
Open Access
Kaynak :
Directory of Open Access Journals (DOAJ)
Dergi:
International Journal of Nanomedicine
Yazar(lar) :
Dai Y , Zhou R , Liu L , Lu Y , Qi J , Wu W
Yıl :
2013
Wu W
Yayıncı Web Sitesi
Kaydet Alıntıla
Yikang Dai,1 Rui Zhou,2 Lin Liu,1 Yi Lu,2 Jianping Qi,2 Wei Wu21Department of Ophthalmology, Renji Hospital, School of Medicine, Shanghai Jiaotong University, 2Key Laboratory of Smart Drug Delivery, Ministry of Education and PLA, School of Pharmacy, Fudan University, Shanghai, People's Republic of ChinaAbstract: The objective of this study was to investigate the potential of liposomes containing bile salts as an ophthalmic delivery system for tacrolimus to improve corneal permeability. Liposomes containing bile salts, including sodium taurocholate, sodium deoxycholate, and sodium glycocholate, were produced by the thin-film dispersion method with a particle size of approximately 100 nm and an entrapment efficiency of more than 90%. Less than 5% tacrolimus was released from conventional liposomes and from liposomes containing sodium taurocholate, sodium deoxycholate, or sodium glycocholate over 12 hours. The cellular uptake of conventional liposomes was significantly higher than that of liposomes containing bile salts. However, liposomes containing bile salts exerted a 3–4-fold increase of tacrolimus in ex vivo corneal transport of tacrolimus compared with conventional liposomes. When rabbit eyes were treated with a DiI perchlorate-loaded liposome suspension, liposomes containing bile salts showed fast and sustained penetration across the cornea. Unfortunately, liposomes containing sodium deoxycholate caused toxicity or irritation to both spontaneously derived human corneal epithelial cells and the rabbit cornea. Therefore, liposomes containing sodium taurocholate and sodium glycocholate are potential carriers in ocular drug delivery systems, given their low toxicity and vastly improved permeability.Keywords: liposomes, bile salt, tacrolimus, cornea, sodium taurocholate, sodium deoxycholate, sodium glycocholate
Cilt: 2013
Sayı: default
Sayfa: 1921 - 1933
Sınıflandırma :
25
Comparison of KRAS and PIK3CA gene status between primary tumors and paired metastases in colorectal cancer
Dergi Makalesi

Dergi Makalesi
Open Access
Kaynak :
Directory of Open Access Journals (DOAJ)
Dergi:
OncoTargets and Therapy
Yazar(lar) :
He Q , Xu Q , Wu W , Chen L , Sun W , Ying JE
Yıl :
2016
Wu W
Yayıncı Web Sitesi
Kaydet Alıntıla
Qiong He,1 Qi Xu,1 Wei Wu,1 Lei Chen,1 Weijing Sun,2 Jieer Ying11Department of Chemotherapy, Zhejiang Cancer Hospital, Hangzhou, People’s Republic of China; 2Department of Gastrointestinal Medical Oncology, School of Medicine, University of Pittsburgh, Pittsburgh, PA, USAPurpose: In metastatic or recurrent colorectal cancer (MRCRC), the concordance of Kirsten rat sarcoma viral oncogene homolog (KRAS) and phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit alpha (PIK3CA) mutation status between the primary tumors and metastases is still controversial. The purpose of this study was to evaluate the association between KRAS and PIK3CA mutational status and various clinicopathologic features, and compare their genotype in primary tumors with that of the paired metastatic tumors.Method: We compared the mutation status of KRAS and PIK3CA between the primary tumors and the paired metastases of 59 MRCRC patients with available tissues (resection or biopsy). The presence of KRAS and PIK3CA mutations were determined by direct sequencing analysis.Results: Seventeen patients (28.8%) had the KRAS mutation and 46 patients (80.0%) had the PIK3CA mutation when considering both the primary and metastatic sites. KRAS mutation was observed in ten primary tumors and eleven related metastases (16.9% vs 18.6%), while PIK3CA mutation was found in 26 primary tumors and 32 related metastases (44.1% vs 54.2%). KRAS status was concordant between primary and metastatic sites in 45 patients (76.3%, kappa =0.157), while the concordance of PIK3CA status was only found in 25 patients (42.4%, kappa =−0.141). The PIK3CA status discordance rate was significantly higher in 40 patients undergoing metachronous resection of primary tumor or metastasis, compared with that in 19 patients with synchronous resection of primary tumor or metastasis (67.5% [27/40] vs 36.8% [7/19]; P=0.026).Conclusion: Our results demonstrate that low concordance of KRAS and high discordance of PIK3CA mutational status exist between the primary tumors and paired metastasis, and these findings remind us to have second thoughts about the need to evaluate metastatic tumors separately rather than only based on the primary tumor data when targeted therapy is considered. Keywords: KRAS, PIK3CA, colorectal cancer, primary tumor, metastatic site
Anahtar Kelimeler : PIK3CA, Colorectal Cancer, primary tumor, metastasis
ISSN: 1178-6930
Cilt: 2016
Sayı: 1
Sayfa: 2329 - 2335
Sınıflandırma :
26
circZNF609 promotes the proliferation and migration of gastric cancer by sponging miR-483-3p and regulating CDK6
Dergi Makalesi

Dergi Makalesi
Open Access
Kaynak :
Directory of Open Access Journals (DOAJ)
Dergi:
OncoTargets and Therapy
Yazar(lar) :
Wu W , Wei N , Shao G , Jiang C , Zhang S , Wang L
Yıl :
2019
Wu W
Yayıncı Web Sitesi
Kaydet Alıntıla
Weidong Wu,1 Ningxian Wei,1 Gang Shao,1 Chunnan Jiang,1 Shaoru Zhang,2 Lihui Wang2 1Anesthesiology Department, Danyang People’s Hospital of Jiangsu Province & Danyang Hospital affiliated to Nantong University, Danyang, Jiangsu 212300, People’s Republic of China; 2Central Laboratory, Danyang People’s Hospital of Jiangsu Province & Danyang Hospital affiliated to Nantong University, Danyang, Jiangsu 212300, People’s Republic of ChinaCorrespondence: Lihui Wang; Shaoru ZhangDanyang People’s Hospital of Jiangsu Province, 2 Xinmin West Road, Danyang 212300, People’s Republic of ChinaEmail njuwanglihui@163.com; zhangshaoru@126.comObjective: To explore the regulatory effects of circZNF609 on proliferative and migratory capacities of gastric cancer (GC) and its underlying mechanism.Methods: Expression level of circZNF609, CDK6 and miR-483-3p in GC tissues and cells were detected qRT-PCR verification. CCK-8 and transwell assay were conducted the cell viability and migratory capacities of GC cells. Dual luciferase assay was enrolled to confirm the interaction among circZNF609, CDK6 and miR-483-3p. Western blot was used to detect the protein level of CDK6.Results: Expression levels of circZNF609 were higher in GC patients by qRT-PCR.GC patients with higher expression of circZNF609 were expected to have a higher TNM stage and lower 5-year survival than those with lower expression. ROC curves showed a well diagnostic value of circZNF609 in GC. Treatment of RNase R in GC cells downregulated the expression of ZNF609, whereas circZNF609 expression did not change. Furthermore, cytoplasmic expression of circZNF609 was higher than those of nuclear expression. Besides, biological experiments indicated that overexpression of circZNF609 promoted the proliferative and migratory capacities of GC cells. To demonstrate the underlying mechanism of circZNF609, we found that circZNF609 bound to miR-483-3p, which presented a lower expression in GC tissues than that of paracancerous tissues. Both circZNF609 and miR-483-3p could bind to Ago2, suggesting that circZNF609 may act as a sponge of miR-483-3p. In addition, the effect of overexpressed circZNF609 on cellular behaviors of GC cells were partly reversed by overexpression of miR-483-3p. Bioinformatics suggested that CDK6 has a potential binding site with miR-483-3p. The expression of CDK6 markedly increased in GC tissues and cells, which was negatively correlated with miR-483-3p expression. Dual-luciferase reporter gene results indicated that miR-483-3p could bind to the 3’-UTR of CDK6. Moreover, miR-483-3p downregulated CDK6 at both mRNA and protein levels. Overexpression of miR-483-3p inhibited proliferative and migratory capacities of GC cells, which were reversed by CDK6 overexpression.Conclusion: In summary, the expression of circZNF609 is upregulated in GC. CircZNF609 can be used as the sponge of miR-483-3p to regulate the expression level of CDK6, thus participating in the progression of GC by regulating the proliferative and migratory capacities of GC cells.Keywords: circZNF609, MiR-483-3p, CDK6, proliferation, migration, gastric cancer
Anahtar Kelimeler : CircZNF609, MiR-483-3p, Proliferation, Migration, Gastric cancer
ISSN: 1178-6930
Cilt: 12
Sayfa: 8197 - 8205
27
Use of clinical characteristics to predict spirometric classification of obstructive lung disease
Dergi Makalesi

Dergi Makalesi
Open Access
Kaynak :
Directory of Open Access Journals (DOAJ)
Dergi:
International Journal of COPD
Yazar(lar) :
Pascoe SJ , Wu W , Collison KA , Nelsen LM , Wurst KE , Lee LA
Yıl :
2018
Wu W
Yayıncı Web Sitesi
Kaydet Alıntıla
Steven J Pascoe,1 Wei Wu,2,3 Kathryn A Collison,1 Linda M Nelsen,4 Keele E Wurst,5 Laurie A Lee6 1Respiratory Medicines Development Center, GSK, Research Triangle Park, NC, USA; 2Biostatistics, PAREXEL International, Research Triangle Park, NC, USA; 3Clinical Statistics, GSK, Research Triangle Park, NC, USA; 4Value Evidence and Outcomes, GSK, Collegeville, PA, USA; 5Epidemiology, GSK, Collegeville, PA, USA; 6Research and Development, GSK, Stevenage, UK Background: There is no consensus on how to define patients with symptoms of asthma and chronic obstructive pulmonary disease (COPD). A diagnosis of asthma–COPD overlap (ACO) syndrome has been proposed, but its value is debated. This study (GSK Study 201703 [NCT02302417]) investigated the ability of statistical modeling approaches to define distinct disease groups in patients with obstructive lung disease (OLD) using medical history and spirometric data.Methods: Patients aged ≥18 years with diagnoses of asthma and/or COPD were categorized into three groups: 1) asthma (nonobstructive; reversible), 2) ACO (obstructive; reversible), and 3) COPD (obstructive; nonreversible). Obstruction was defined as a post-bronchodilator forced expiratory volume in 1 second (FEV1)/forced vital capacity <0.7, and reversibility as a post-albuterol increase in FEV1 ≥200 mL and ≥12%. A primary model (PM), based on patients’ responses to a health care practitioner-administered questionnaire, was developed using multinomial logistic regression modeling. Other multivariate statistical analysis models for identifying asthma and COPD as distinct entities were developed and assessed using receiver operating characteristic (ROC) analysis. Partial least squares discriminant analysis (PLS-DA) assessed the degree of overlap between groups.Results: The PM predicted spirometric classifications with modest sensitivity. Other analysis models performed with high discrimination (area under the ROC curve: asthma model, 0.94; COPD model, 0.87). PLS-DA identified distinct phenotypic groups corresponding to asthma and COPD.Conclusion: Within the OLD spectrum, patients with asthma or COPD can be identified as two distinct groups with a high degree of precision. Patients outside these classifications do not constitute a homogeneous group. Keywords: asthma–COPD overlap syndrome, asthma, COPD, differential diagnosis, surveys and questionnaires
Anahtar Kelimeler : Asthma-COPD overlap syndrome, Asthma, differential diagnosis, surveys and questionnaires
Cilt: 13
Sayfa: 889 - 902
28
Interactive effects of salinity and low potassium on growth, physiology response of Houttuynia cordata Thunb. W01-100
Dergi Makalesi

Dergi Makalesi
Open Access
Kaynak :
Directory of Open Access Journals (DOAJ)
Dergi:
Journal of Stress Physiology & Biochemistry
Yazar(lar) :
Zou Y. T. , Dai S. , Li J. Y. , Liu Z. Q. , Wu W.
Yıl :
2012
Wu W.
Tam Metin
Kaydet Alıntıla
Houttuynia cordata Thunb. is a plant enrichment in potassium in plant was reported. Salinity and low potassium availability are important environmental factors restricting plant growth and productivity throughout the world. The interactive effects of salinity and potassium on growth, water content, chlorophyll content, lipid peroxidation content, ion accumulations and K+/Na+ ratio, and organic accumulations as well as oxidative enzymes were investigated in Houttuynia cordata Thunb.. Plants of three-leaf-stage were selected for uniformity, then treated with four levels of Na+ (50, 100, 200 mmol/L) and K+(0, 0.6, 1.2, 2.4 mmol/L) for 20 days. Plant biomass production, ratio of root and shoot, root numbers, water content and MDA content significantly declined in the combined effect of salinity and K+ deprivation, and increased with salinity. However, salinity in conjunction with K+ deprivation led to an increase on leaf chlorophyll content, which even increased with increasing salinity levels. As expected, K+ content in plant was positive correlated with supplementary K+ concentrations, while Na+ was well correlated with salinity, especially enhanced by the interactive effects of salinity and K+ deprivation. Soluble sugar and proline contents remarkable increased by the highest salinity. SOD activity also substantial increased by the highest salinity, and increased with supplementary K+ concentrations. However, elevated CAT and POD activities were not accompanied with an increase in SOD activity.
Anahtar Kelimeler : salinity, potassium, ion contents, organic accumulation, antioxidant enzymes
ISSN: 1997-0838
Cilt: 8
Sayı: 4
Sayfa: 130 - 142
Sınıflandırma :
29
Zinc oxide nanoparticle-induced atherosclerotic alterations in vitro and in vivo
Dergi Makalesi

Dergi Makalesi
Open Access
Kaynak :
Directory of Open Access Journals (DOAJ)
Dergi:
International Journal of Nanomedicine
Yazar(lar) :
Yan Z , Wang W , Wu Y , Wang W , Li B , Liang N , Wu W
Yıl :
2017
Wu W
Yayıncı Web Sitesi
Kaydet Alıntıla
Zhen Yan,1,* Wenjun Wang,2,* Yongjun Wu,1,* Wei Wang,2 Bing Li,1 Ning Liang,1 Weidong Wu3 1College of Public Health, Zhengzhou University, Zhengzhou, 2School of Public Health, Jining Medical University, Jining, 3School of Public Health, Xinxiang Medical University, Xinxiang, People’s Republic of China *These authors contributed equally to this work Abstract: Engineered zinc oxide nanoparticles (ZnO-NPs) are currently being produced in high tonnage. Exposure to ZnO-NPs presents potential risks to cardiovascular system. Thus far, the toxicological effects of ZnO-NPs on cardiovascular system have not been well characterized. In this study, human coronary artery endothelial cells (HCAECs) were exposed to ZnO-NPs directly or indirectly using a transwell coculture system with human alveolar epithelial cell line A549 to mimic the lung/circulation interaction. It was shown that levels of proinflammatory mediators (interleukin-8 [IL-8] and tumor necrosis factor-α [TNF-α]) and biomarkers of atherosclerogenesis (heme oxygenase-1 [HO-1] and platelet endothelial cell adhesion molecules-1 [PECAM-1]) in the supernatants of culture media were significantly increased. Pretreatment of A549 cells on the apical side of the coculture system with the phagocytosis inhibitor cytochalasin B (CB) blocked ZnO-NP-induced HO-1 and PECAM-1 expression in HCAEC, indicating that endocytosis of ZnO-NPs by alveolar epithelial cells was involved in ZnO-NP-induced HO-1 or PECAM-1 expression in endothelial cells. Moreover, Wistar rats were intratracheally instilled with ZnO-NP suspension and high fat diet (positive control). ZnO-NP treatment induced lung and systemic inflammation, dyslipidemia, increased levels of serum HO-1 and PECAM-1, and aortic pathological damage. Taken together, exposure to ZnO-NPs could induce atherosclerotic alterations, which might involve phagocytosis of nanoparticles and inflammation in the lung. Keywords: zinc oxide nanoparticles, atherosclerosis, lung inflammation, heme oxygenase-1, platelet endothelial cell adhesion molecules-1
Anahtar Kelimeler : Zinc oxide nanoparticles, atherosclerosis, lung inflammation, heme oxygenase-1, platelet endothelial cell adhesion molecules-1
Cilt: 12
Sayfa: 4433 - 4442
Sınıflandırma :
30
Design and Analysis of a Micromechanical Three-Component Force Sensor for Characterizing and Quantifying Surface Roughness
Dergi Makalesi

Dergi Makalesi
Open Access
Kaynak :
Directory of Open Access Journals (DOAJ)
Dergi:
Measurement Science Review
Yazar(lar) :
Liang Q. , Wu W. , Zhang D. , Wei B. , Sun W. , Wang Y. , Ge Y.
Yıl :
2015
Wu W.
Yayıncı Web Sitesi
 Piri Tam Metin Bulucu
Kaydet Alıntıla
Roughness, which can represent the trade-off between manufacturing cost and performance of mechanical components, is a critical predictor of cracks, corrosion and fatigue damage. In order to measure polished or super-finished surfaces, a novel touch probe based on three-component force sensor for characterizing and quantifying surface roughness is proposed by using silicon micromachining technology. The sensor design is based on a cross-beam structure, which ensures that the system possesses high sensitivity and low coupling. The results show that the proposed sensor possesses high sensitivity, low coupling error, and temperature compensation function. The proposed system can be used to investigate micromechanical structures with nanometer accuracy.
DOI : 10.1515/msr-2015-0034
Anahtar Kelimeler : Surface roughness metrology, multi-component force sensor, Finite element analysis
ISSN: 1335-8871
Cilt: 15
Sayı: 5
Sayfa: 248 - 255
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