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1
Simple reverse genetics systems for Asian and African Zika viruses
Dergi Makalesi

Dergi Makalesi
Kaynak :
Springer Nature
Dergi:
Scientific Reports
Yayıncı:
Springer Science and Business Media LLC
Yazar(lar) :
Thérèse Atieh , Cécile Baronti , Xavier de Lamballerie , Antoine NougairèdeThérèse Atieh , Cécile Baronti , Xavier de Lamballerie , Antoine Nougairède
Yıl :
2016
Xavier de Lamballerie Xavier de Lamballerie
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DOI : 10.1038/srep39384
ISSN: 2045-2322
Sayı: 1
Cilt: 6
Sınıflandırma :
2
Chikungunya disease and chloroquine treatment
Dergi Makalesi

Dergi Makalesi
Kaynak :
Wiley Online Library
Dergi:
Journal of Medical Virology
Yazar(lar) :
Ilenia Delogu , Xavier de Lamballerie
Konu :
Virology, Infectious Diseases
Yıl :
2011
Xavier de Lamballerie
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DOI : 10.1002/jmv.22019
ISSN: 0146-6615
Sayı: 6
Cilt: 83
Sayfa: 1058-1059
3
The 1932 Macau epidemic of cerebrospinal meningitis: A historical perspective and critical review of the data
Dergi Makalesi

Dergi Makalesi
Kaynak :
Science Direct
Dergi:
Infection, Genetics and Evolution
Yayıncı:
Elsevier
Yazar(lar) :
Dominique Buchillet , Xavier de Lamballerie
Yıl :
2010
Xavier de Lamballerie
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Since the first clinical description by Vieusseux (1805) of the epidemic form of meningitis known today as cerebrospinal meningitis, numerous epidemic outbreaks of the disease were reported globally during the nineteenth and early twentieth century. Historical medical data confirmed that clinical disease may occur either sporadically or in an epidemic form. Moreover, it may afflict children, young military recruits and/or populations living under crowded conditions. In 1932, an epidemic of meningitis occurred in Macau. The disease was sufficiently unusual to justify the publication of a special report by the Portuguese physician in charge of the control services of the epidemic. Here we present a critical review of the Macau epidemic data.
DOI : 10.1016/j.meegid.2010.05.012
Anahtar Kelimeler : Cerebrospinal meningitis, History of meningitis epidemics, Epidemiology, Macau, South China
ISSN: 1567-1348
Sayı: 7
Cilt: 10
Sayfa: 896-902
Sınıflandırma :
4
Of chloroquine and COVID-19
Dergi Makalesi

Dergi Makalesi
Kaynak :
Science Direct
Dergi:
Antiviral Research
Yayıncı:
Elsevier
Yazar(lar) :
Franck Touret , Xavier de Lamballerie
Yıl :
2020
Xavier de Lamballerie
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Recent publications have brought attention to the possible benefit of chloroquine, a broadly used antimalarial drug, in the treatment of patients infected by the novel emerged coronavirus (SARS-CoV-2). The scientific community should consider this information in light of previous experiments with chloroquine in the field of antiviral research.
DOI : 10.1016/j.antiviral.2020.104762
Anahtar Kelimeler : SARS-CoV-2, COVID-19, 2019-nCoV, Chloroquine, Antiviral
ISSN: 0166-3542
Cilt: 177
Sayfa: 104762
Sınıflandırma :
5
Arenaviruses other than Lassa virus
Dergi Makalesi

Dergi Makalesi
Kaynak :
Science Direct
Dergi:
Antiviral Research
Yayıncı:
Elsevier
Yazar(lar) :
Rémi N Charrel , Xavier de Lamballerie
Yıl :
2003
Xavier de Lamballerie
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The family Arenaviridae includes 23 viral species, of which 5 can cause viral hemorrhagic fevers with a case fatality rate of about 20%. These five viruses are Junin, Machupo, Guanarito, Sabia and Lassa virus, the manipulation of which requires biosafety level 4 facilities. They are included in the Category A Pathogen List established by the Center for Disease Control and Prevention that groups agents with the greatest potential for adverse public health impact and mass casualties whether a situation characterized by a ill-intentioned abuse of natural or engineered arenavirus would be encountered. The aims of this article are to (i) summarize the current situation; (ii) provide information to help anticipating the effects to be expected in such a situation; and to (iii) emphasize the need for fundamental research to allow the development of diagnostic, prevention and therapeutic tools as countermeasures to weaponized arenaviruses.
DOI : 10.1016/s0166-3542(02)00202-4
Anahtar Kelimeler : Arenavirus, Bioterrorism, Viral hemorrhagic fever, Lassa virus, Guanarito virus, Machupo virus, Junin virus, Sabia virus, Ribavirin
ISSN: 0166-3542
Sayı: 1-2
Cilt: 57
Sayfa: 89-100
Sınıflandırma :
6
With reference to Mexican flu
Dergi Makalesi

Dergi Makalesi
Kaynak :
Wiley Online Library
Dergi:
Influenza and Other Respiratory Viruses
Yazar(lar) :
Xavier de Lamballerie , Ernest A. Gould
Konu :
Public Health, Environmental and Occupational Health, Epidemiology, Pulmonary and Respiratory Medicine, Infectious Diseases
Yıl :
2009
Xavier de Lamballerie
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DOI : 10.1111/j.1750-2659.2009.00097.x
ISSN: 1750-2640
Sayı: 5
Cilt: 3
Sayfa: 203-203
7
Le virus West Nile, un arbovirus émergent
Dergi Makalesi

Dergi Makalesi
Kaynak :
Science Direct
Dergi:
La Presse Médicale
Yayıncı:
Elsevier
Yazar(lar) :
Rémi N. Charrel , Xavier de Lamballerie
Yıl :
2004
Xavier de Lamballerie
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Résumé Le virus West Nile (famille Flaviviridae, genre Flavivirus), isolé pour la première fois en Afrique en 1937, est un arbovirus transmis par la piqûre d’un moustique du genre Culex. Son cycle naturel comporte un vecteur (moustique du genre Culex), un réservoir (oiseaux de diverses espèces) ; les hommes et les grands mammifères représentent des impasses épidémiologiques. Une dissémination progressive Ce virus a attiré l’attention par plusieurs épidémies et épizooties en France, en Russie, en Roumanie, en Israël. L’événement le plus marquant est son introduction en 1999 dans le Nord-Est des États-Unis, territoire préalablement exempt du virus West Nile, et sa dissémination progressive suivant des gradients Nord-Sud et Ouest-Est pour réaliser fin 2002 une occupation de la quasi-totalité du territoire nord-américain (États-Unis, Canada et Mexique). L’année 2002 a été particulièrement favorable à la dissémination du virus West Nile aux États-Unis avec l’enregistrement de plus de 4 000 cas humains et de près de 300 morts. En 2003, près de 10 000 cas ont été enregistrés. De janvier à octobre 2004, près de 2 000 cas humains ont été enregistrés. Parmi les patients infectés par le virus, une minorité développera une forme symptomatique et seule une faible partie de ces derniers aura une forme neurologique. Pour le diagnostic et la surveillance Bien que la technique de référence soit toujours l’isolement du virus, les méthodes diagnostiques utilisées en routine reposent essentiellement sur les techniques sérologiques (détection d’une augmentation du taux des anticorps spécifiques sur 2 sérums prélevés à 21 jours d’intervalle, détection des IgM sur un sérum unique) et sur les techniques moléculaires (détection du génome du virus) ; ces dernières ont été développées dans des formats quantitatifs en temps réel permettant un rendu de résultat dans les 24 heures. Nous décrivons la situation en France à la suite de l’épizootie équine survenue en 2000 et les modalités de la surveillance mise en place à cette occasion. Summary The West Nile virus (family Flaviviridae, genus Flavivirus), first isolated in 1937 in Africa, is an arthropod-borne virus transmitted through Culex mosquitoes bite. Its natural cycle includes a vector (Culex mosquitoes), a reservoir (birds of various species) and humans and large mammals are epidemiological dead-ends. Progressive dissemination This virus recently drew attention after several epidemics and epizooties were identified in France, Russia, Romania, and Israel. However, the most striking fact is its introduction in 1999 into the north-eastern United States, a country previously West Nile virus-free, and its progressive spread according to North-South and West-East gradients to finally cover the near-totality of the north American territory (USA, Canada and Mexico), in 2002. The Year 2002 has been particularly prone to disseminating West Nile virus in the Unites States: more than 4 000 human cases have been recorded and resulted in the death of almost 300. In 2003, around 10 000 cases were recorded. From January to October 2004, around 2 000 human cases have been recorded in the USA. Among infected patients, few develop a symptomatic form, and only a small proportion of the latter will develop a neurological form. Regarding diagnosis and surveillance Although virus isolation remains the technique of reference, routine diagnosis basically relies on serology (detection of increased levels of specific antibodies on two blood tests at 21 days interval, detection of IgM on a single test) and on molecular techniques (detection of the viral genome); the latter have been developed in quantitative and real-time formats that provide results within 24 hours. We describe the situation in France following the equine epizooty that occurred in 2000, and the surveillance modalities initiated at that time.
DOI : 10.1016/s0755-4982(04)98977-4
ISSN: 0755-4982
Sayı: 21
Cilt: 33
Sayfa: 1521-1526
Sınıflandırma :
8
Biosafety and Biosecurity in European Containment Level 3 Laboratories: Focus on French Recent Progress and Essential Requirements
Dergi Makalesi

Dergi Makalesi
Open Access
Kaynak :
Directory of Open Access Journals (DOAJ)
Dergi:
Frontiers in Public Health
Yazar(lar) :
Boris Pastorino , Boris Pastorino , Xavier de Lamballerie , Xavier de Lamballerie , Rémi Charrel , Rémi Charrel
Yıl :
2017
Xavier de Lamballerie Xavier de Lamballerie
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Kaydet Alıntıla
Even if European Union (EU) Member States are obliged to implement EU Directives 2000/54/EC on the protection of workers from risks related to exposure to biological agents at work, national biosafety regulations and practices varied from country to country. In fact, EU legislation on biological agents and genetically modified microorganisms is often not specific enough to ensure harmonization leading to difficulties in implementation for most laboratories. In the same way, biosecurity is a relatively new concept and a few EU Member States are known to have introduced national laboratory biosecurity legislation. In France, recent regulations have reinforced biosafety/biosecurity in containment level 3 (CL-3) laboratories but they concern a specific list of pathogens with no correlation in other European Members States. The objective of this review was to summarize European biosafety/biosecurity measures concerning CL-3 facilities focusing on French specificities. Essential requirements needed to preserve efficient biosafety measures when manipulating risk group 3 biological agents are highlighted. In addition, International, European and French standards related to containment laboratory planning, operation or biosafety equipment are described to clarify optimal biosafety and biosecurity requirements.
DOI : 10.3389/fpubh.2017.00121
Anahtar Kelimeler : biosafety, BSL-3, European union, regulations, infectious disease transmission, vertical
ISSN: 2296-2565
Cilt: 5
Sınıflandırma :
9
Evolution of Chikungunya virus in mosquito cells.
Dergi Makalesi

Dergi Makalesi
Open Access
Kaynak :
PubMed
Dergi:
Sci Rep
Yazar(lar) :
Souand Mohamed Ali , Abdennour Amroun , Xavier de Lamballerie , Antoine Nougairède
Yıl :
2018
Xavier de Lamballerie
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Kaydet Alıntıla
It has been observed that replication of Chikungunya virus (CHIKV) in C6/36 Aedes albopictus cells has little effect on virus evolution. To characterize evolutionary patterns associated with CHIKV replication in mosquito cells, we performed serial passages of the LR2006 strain in Ae. albopictus cells (75 and 30 passages in C6/36 and U4.4 respectively) and Ae. aegypti cells (100 passages in AA-A20 and in AE) and studied genotypic changes accompanying adaptation during this evolutionary process. Quantitative analysis revealed cell specific patterns: low mutation rates in C6/36 cells except when a CHIKV strain pre-adapted to mammalian was used and typical features of adaptation to cell culture conditions with a high number of fixed mutations in AE and AA-A20 cells probably due to the weak permissiveness of these latter cell lines. Altogether, these results suggested that both cell line and viral strain influence rates of viral evolution. In contrast, characteristics and distribution of mutations were qualitatively very similar in all mosquito cells with a high level of parallel evolution including 4 deletion mutations. Serial passage in mammalian cells of viruses pre-adapted to mosquito cells revealed disappearance of almost all shared mutations suggesting that many of these mutational patterns are vector-specific.
DOI : 10.1038/s41598-018-34561-x
Sınıflandırma :
Viroloji %100
10
Effective detection of highly divergent viral genomes in infected cell lines using a new subtraction strategy (primer extension enrichment reaction—PEER)
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Dergi Makalesi
Kaynak :
Science Direct
Dergi:
Journal of Virological Methods
Yayıncı:
Elsevier
Yazar(lar) :
Philippe Biagini , Xavier de Lamballerie , Philippe de Micco
Yıl :
2007
Xavier de Lamballerie
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The development of new molecular approaches for identification or discovery of known and unknown pathogens is essential. The purpose of this study was to evaluate the utility of a new method designated primer extension enrichment reaction (PEER) in detecting viral genomes from highly divergent viral families (ssDNA, dsDNA, ssRNA, dsRNA) in infected cell lines. Findings indicate that the PEER methodology is a powerful tool since it permitted successful ex vivo detection of infectious agents from the Circoviridae, Herpesviridae, Paramyxoviridae, Picornaviridae and Reoviridae families. The great potential of PEER was further confirmed in tests using serial dilutions of West Nile virus (Flaviviridae) and in a “blind” test in which a member of the Poxviridae family was successfully identified. The technique was shown to be useful for identifying causative viral pathogens, following inoculation of susceptible cell lines with clinical biological samples.
DOI : 10.1016/j.jviromet.2006.09.010
Anahtar Kelimeler : Subtraction technique, Nucleic acids, PEER, Virus, Cell line
ISSN: 0166-0934
Sayı: 1
Cilt: 139
Sayfa: 106-110
Sınıflandırma :
Viroloji %89
11
G + C content differs in conserved and variable amino acid residues of flaviviruses and other evolutionary groups
Dergi Makalesi

Dergi Makalesi
Kaynak :
Science Direct
Dergi:
Infection, Genetics and Evolution
Yayıncı:
Elsevier
Yazar(lar) :
Raphaëlle Klitting , Ernest Andrew Gould , Xavier de Lamballerie
Yıl :
2016
Xavier de Lamballerie
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Flaviviruses are small RNA viruses that exhibit genetic and ecological diversity and a wide range of G + C content (GC%). We discovered that, amongst flaviviruses, the GC% of nucleotides encoding conserved amino acid (AA) residues was consistently higher than that of nucleotides encoding variable AAs. This intriguing phenomenon was also identified for a wide range of other viruses, and some non-viral evolutionary groups. Here, we analyse the possible mechanisms underlying this imbalanced nucleotide content (in particular the role of the specific G content and the AA composition in flaviviral genomes) and discuss its evolutionary implications. Our findings suggest that one of the most simple characteristics of the genetic code (i.e., the G or G + C content of codons) is linked with the evolutionary behavior of the corresponding encoded AAs.
DOI : 10.1016/j.meegid.2016.09.017
Anahtar Kelimeler : AA Amino Acid, Amino Acid, APOIV Apoi virus, Apoi virus, CA-Cons Complete Alignment Conserved, Complete Alignment Conserved, CA-Var Complete Alignment Variable, Complete Alignment Variable, CDS complete coding sequences, complete coding sequences, CXFV Culex-borne flavivirus, Culex-borne flavivirus, DENV dengue virus, dengue virus, GC% G + C content, G + C content, ISFV insect specific flaviviruses, insect specific flaviviruses, JEV Japanese encephalitis virus, Japanese encephalitis virus, MBFV mosquito-borne flaviviruses, mosquito-borne flaviviruses, MODV Modoc virus, Modoc virus, MMLV Montana Myotis Leukoencephalitis virus, Montana Myotis Leukoencephalitis virus, NKV viruses with no known vector, viruses with no known vector, ORF open reading frame, open reading frame, PW pairwise, pairwise, RBV Rio Bravo virus, Rio Bravo virus, TBEV tick-borne encephalitis virus, tick-borne encephalitis virus, TBFV tick-borne flaviviruses, tick-borne flaviviruses, WNV West Nile virus, West Nile virus, YFV yellow fever virus, yellow fever virus, Flavivirus, Evolution, G + C content
ISSN: 1567-1348
Cilt: 45
Sayfa: 332-340
Sınıflandırma :
12
Molecular determinants of Yellow Fever Virus pathogenicity in Syrian Golden Hamsters: one mutation away from virulence.
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Dergi Makalesi
Open Access
Kaynak :
PubMed
Dergi:
Emerg Microbes Infect
Yazar(lar) :
Raphaëlle Klitting , Laura Roth , Félix A Rey , Xavier de Lamballerie
Konu :
Animals, Cricetinae, Humans, Liver, Mutation, Reverse Genetics, Viral Envelope Proteins, Viral Load, Virulence, Virus Replication, Yellow Fever, Yellow fever virus
Yıl :
2018
Xavier de Lamballerie
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Yellow fever virus (Flavivirus genus) is an arthropod-borne pathogen, which can infect humans, causing a severe viscerotropic disease with a high mortality rate. Adapted viral strains allow the reproduction of yellow fever disease in hamsters with features similar to the human disease. Here, we used the Infectious Subgenomic Amplicons reverse genetics method to produce an equivalent to the hamster-virulent strain, Yellow Fever Ap7, by introducing a set of four synonymous and six nonsynonymous mutations into a single subgenomic amplicon, derived from the sequence of the Asibi strain. The resulting strain, Yellow Fever Ap7M, induced a disease similar to that described for Ap7 in terms of symptoms, weight evolution, viral loads in the liver and lethality. Using the same methodology, we produced mutant strains derived from either Ap7M or Asibi viruses and investigated the role of each of Ap7M nonsynonymous mutations in its in vivo phenotype. This allowed identifying key components of the virulence mechanism in hamsters. In Ap7M virus, the reversion of either E/Q27H or E/D155A mutations led to an important reduction of both virulence and in vivo replicative fitness. In addition, the introduction of the single D155A Ap7M mutation within the E protein of the Asibi virus was sufficient to drastically modify its phenotype in hamsters toward both a greater replication efficiency and virulence. Finally, inspection of the Asibi strain E protein structure combined to in vivo testing revealed the importance of an exposed α-helix in domain I, containing residues 154 and 155, for Ap7M virulence in hamsters.
DOI : 10.1038/s41426-018-0053-x
Sınıflandırma :
Biyotıp %100
Viroloji %100
13
What Does the Future Hold for Yellow Fever Virus? (I).
Dergi Makalesi

Dergi Makalesi
Open Access
Kaynak 1:
PubMed
Kaynak 2:
MDPI
Kaynak 3:
Directory of Open Access Journals (DOAJ)
Dergi:
Genes (Basel)
Yazar(lar) :
Raphaëlle Klitting , Ernest A Gould , Christophe Paupy , Xavier de Lamballerie
Yıl :
2018
Xavier de Lamballerie
Piri Tam Metin Bulucu
Kaydet Alıntıla
The recent resurgence of yellow fever virus (YFV) activity in the tropical regions of Africa and South America has sparked renewed interest in this infamous arboviral disease. Yellow fever virus had been a human plague for centuries prior to the identification of its urban transmission vector, the Aedes (Stegomyia) aegypti (Linnaeus) mosquito species, and the development of an efficient live-attenuated vaccine, the YF-17D strain. The combination of vector-control measures and vaccination campaigns drastically reduced YFV incidence in humans on many occasions, but the virus never ceased to circulate in the forest, through its sylvatic invertebrate vector(s) and vertebrate host(s). Outbreaks recently reported in Central Africa (2015⁻2016) and Brazil (since late 2016), reached considerable proportions in terms of spatial distribution and total numbers of cases, with multiple exports, including to China. In turn, questions about the likeliness of occurrence of large urban YFV outbreaks in the Americas or of a successful import of YFV to Asia are currently resurfacing. This two-part review describes the current state of knowledge and gaps regarding the molecular biology and transmission dynamics of YFV, along with an overview of the tools that can be used to manage the disease at individual, local and global levels.
DOI : 10.3390/genes9060291
Anahtar Kelimeler : emergence, flavivirus, vector-borne transmission, yellow fever virus
Sınıflandırma :
14
Seasonality of mosquitoes and chikungunya in Italy
Dergi Makalesi

Dergi Makalesi
Kaynak :
Science Direct
Dergi:
The Lancet Infectious Diseases
Yayıncı:
Elsevier
Yazar(lar) :
Rémi N Charrel , Xavier de Lamballerie , Didier Raoult
Yıl :
2008
Xavier de Lamballerie
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DOI : 10.1016/s1473-3099(07)70296-7
ISSN: 1473-3099
Sayı: 1
Cilt: 8
Sayfa: 5-6
Sınıflandırma :
15
Phylogeny of the genus Arenavirus
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Dergi Makalesi
Kaynak :
Science Direct
Dergi:
Current Opinion in Microbiology
Yayıncı:
Elsevier
Yazar(lar) :
Rémi N Charrel , Xavier de Lamballerie , Sébastien Emonet
Yıl :
2008
Xavier de Lamballerie
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The family Arenaviridae consists of a unique genus (Arenavirus) that currently comprises 22 viral species, as recognized by the International Committee for Taxonomy of Viruses. Seven newly discovered represent putative new species. Here, our aims were to provide the most comprehensive phylogenetic analysis of members and putative members of the family Arenaviridae to date, and to investigate the genetic diversity observed within and between recognized species of New world arenaviruses to determine whether the genetic criteria previously proposed to define arenavirus species for Old world arenaviruses should be retained or are more widely applicable to the whole genus.
DOI : 10.1016/j.mib.2008.06.001
ISSN: 1369-5274
Sayı: 4
Cilt: 11
Sayfa: 362-368
Sınıflandırma :
16
First molecular detection of Rickettsia africae in a tropical bont tick, Amblyomma variegatum, collected in Corsica, France
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Dergi Makalesi
Kaynak :
Springer Link
Dergi:
Experimental and Applied Acarology
Yazar(lar) :
Vincent Cicculli , Xavier de Lamballerie , Remi Charrel , Alessandra Falchi
Konu :
Insect Science, Ecology, General Medicine
Yıl :
2019
Xavier de Lamballerie
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Here we report the first detection of Amblyomma variegatum, a tick species of medical and veterinary importance, and the first molecular evidence of a pathogen, Rickettsia africae, both new to Corsica (France). In August 2018, an ixodid tick with an unusual morphology was removed from the ventral part of a cow’s whole skin in a slaughterhouse located in the village of Ponte-Leccia (Haute-Corse). The tick was morphologically identified as an adult male of A. variegatum. This result was confirmed by 16S rDNA sequence analysis with a close relative being a sequence from Senegal showing 99% nucleotide identity. We tested the tick for Ehrlichia and Rickettsia. The tick was positive to Rickettsia and the corresponding sequence matched with R. africae. There is little or no risk of the introduction and establishment of a viable population of A. variegatum in Corsica by migrating birds. However, if it did, it could produce major economic losses for livestock production. Further studies and sustained surveillance are indicated, not only focusing on this species of tick and this rickettsia, but also on other microorganisms of veterinary and medical importance that might be transmitted in Corsica and other Mediterranean islands.
DOI : 10.1007/s10493-018-00336-2
Anahtar Kelimeler : Amblyomma variegatum, Rickettsia africae, Cattle, Corsica, Mediteranean basin
ISSN: 0168-8162 1572-9702
Sayı: 2
Cilt: 77
Sayfa: 207-214
17
Système de génétique inverse appliqué au génotype 3 du virus de l’hépatite E européen
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Dergi Makalesi
Kaynak :
Science Direct
Dergi:
Transfusion Clinique et Biologique
Yayıncı:
Elsevier
Yazar(lar) :
Mhammed Touinssi , Antoine Nougairede , Xavier De Lamballerie , Pierre Gallian
Yıl :
2017
Xavier De Lamballerie
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Le virus de l’hépatite E (VHE) est, à l’heure actuelle, un des pathogènes au centre des considérations liées à la sécurité des produits sanguins. Ce virus nu, possède un génome à ARN monocaténaire, de polarité positive, d’une taille d’environ 7,2 kb. Le génotype 3 étant majoritairement présent en Europe. Son caractère virémique persistant 4 à 8 semaines et le recensement de cas avérés de transmission par transfusion justifient l’intérêt pour ce virus. Les premières études ont confirmé la présence du virus dans le sang d’individus sains (∼0.045 % en France) et une assez forte séroprévalence (parfois > 50 %) selon les régions étudiées. Nous proposons de réaliser ce projet en deux étapes : –Parce que la population Corse est considérée véritablement comme une population à risque, nous projetons de tester des échantillons de donneurs de sang pour le VHE dans cette région. L’objectif étant d’avoir des échantillons positifs avec une charge virale élevée, ce qui nous sera d’une grande utilité pour nos futures études ; –Notre approche moléculaire consiste à appliquer la technique ISA (Infectious Subgenomic Amplicons), le but étant de récupérer un virus infectieux de l’hépatite E. Ainsi, l’ensemble du génome viral du VHE (subdivisé en 3 fragments d’ADN double brin chevauchants) flanqué dans les régions 5’et 3’ non traduites par le pCMV et le HDR/SV40pA, respectivement, a été synthétisé par GenScript, États-Unis. Chacun de ces fragments sera ensuite amplifié par PCR et tous les produits subgénomiques seront regroupés et transfectés dans des cellules permissives pour générer des VHE infectieux. Les travaux sont en cours et le projet de recherche sera dirigé en fonction des résultats obtenus.
DOI : 10.1016/j.tracli.2017.06.240
ISSN: 1246-7820
Sayı: 3
Cilt: 24
Sayfa: 357
Sınıflandırma :
18
New reverse genetics and transfection methods to rescue arboviruses in mosquito cells
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Dergi Makalesi
Kaynak :
Springer Nature
Dergi:
Scientific Reports
Yayıncı:
Springer Science and Business Media LLC
Yazar(lar) :
Thérèse Atieh , Antoine Nougairède , Raphaëlle Klitting , Fabien Aubry , Anna-Bella Failloux , Xavier de Lamballerie , Stéphane PrietThérèse Atieh , Antoine Nougairède , Raphaëlle Klitting , Fabien Aubry , Anna-Bella Failloux , Xavier de Lamballerie , Stéphane Priet
Yıl :
2017
Xavier de Lamballerie Xavier de Lamballerie
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DOI : 10.1038/s41598-017-14522-6
ISSN: 2045-2322
Sayı: 1
Cilt: 7
19
Prevalence of antibodies to lymphocytic choriomeningitis virus in blood donors in southeastern France
Dergi Makalesi

Dergi Makalesi
Kaynak :
Wiley Online Library
Dergi:
Transfusion
Yazar(lar) :
Xavier de Lamballerie , Charles F. Fulhorst , Remi N. Charrel
Konu :
Immunology, Immunology and Allergy, Hematology
Yıl :
2007
Xavier de Lamballerie
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DOI : 10.1111/j.1537-2995.2007.01081.x
ISSN: 0041-1132
Sayı: 1
Cilt: 47
Sayfa: 172-173
20
TT virus and TT virus-like mini-virus infection in French blood donors
Dergi Makalesi

Dergi Makalesi
Kaynak :
Wiley Online Library
Dergi:
Transfusion
Yazar(lar) :
Philippe Biagini , Pierre Gallian , Philippe de Micco , Xavier de Lamballerie
Yıl :
2000
Xavier de Lamballerie
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Kaydet Alıntıla
DOI : 10.1046/j.1537-2995.2000.40121542.x
ISSN: 0041-1132
Sayı: 12
Cilt: 40
Sayfa: 1542-1542
21
Flavivirus reverse genetic systems, construction techniques and applications: A historical perspective
Dergi Makalesi

Dergi Makalesi
Kaynak :
Science Direct
Dergi:
Antiviral Research
Yayıncı:
Elsevier
Yazar(lar) :
Fabien Aubry , Antoine Nougairède , Ernest A. Gould , Xavier de Lamballerie
Yıl :
2015
Xavier de Lamballerie
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Kaydet Alıntıla
The study of flaviviruses, which cause some of the most important emerging tropical and sub-tropical human arbovirus diseases, has greatly benefited from the use of reverse genetic systems since its first development for yellow fever virus in 1989. Reverse genetics technology has completely revolutionized the study of these viruses, making it possible to manipulate their genomes and evaluate the direct effects of these changes on their biology and pathogenesis. The most commonly used reverse genetics system is the infectious clone technology. Whilst flavivirus infectious clones provide a powerful tool, their construction as full-length cDNA molecules in bacterial vectors can be problematic, laborious and time consuming, because they are often unstable, contain unwanted induced substitutions and may be toxic for bacteria due to viral protein expression. The incredible technological advances that have been made during the past 30 years, such as the use of PCR or new sequencing methods, have allowed the development of new approaches to improve preexisting systems or elaborate new strategies that overcome these problems. This review summarizes the evolution and major technical breakthroughs in the development of flavivirus reverse genetics technologies and their application to the further understanding and control of these viruses and their diseases.
DOI : 10.1016/j.antiviral.2014.12.007
Anahtar Kelimeler : Flavivirus, Reverse genetics, Infectious clone
ISSN: 0166-3542
Cilt: 114
Sayfa: 67-85
Sınıflandırma :
Viroloji %96
22
Nonstructural NS1 proteins of several mosquito-borne Flavivirus do not inhibit TLR3 signaling
Dergi Makalesi

Dergi Makalesi
Kaynak :
Science Direct
Dergi:
Virology
Yayıncı:
Elsevier
Yazar(lar) :
Cécile Baronti , Joséphine Sire , Xavier de Lamballerie , Gilles Quérat
Yıl :
2010
Xavier de Lamballerie
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Flaviviruses are single-stranded positive RNA viruses that replicate through double stranded RNA (dsRNA) intermediates. These dsRNA may be recognized as pathogen-associated molecular patterns by cellular receptors including membrane-bound Toll-like receptor 3 (TLR3) and cytosolic helicases RIG-I and MDA5. dsRNA stimulation results in signaling cascades converging to activation of interferon (IFN) regulatory factor 3 (IRF3) and to transcriptional activation of several interferon stimulated genes, including IFNß and inflammatory cytokines. There are conflicting reports concerning the ability of West Nile virus to counteract TLR3 signaling. In our analyses, transiently or stably expressed NS1 proteins from two West Nile viruses, two dengue 2 viruses and a yellow fever virus failed to inhibit TLR3 signaling in two different mammalian cell lines. Moreover, using siRNA inhibiting the helicase signalization pathway, we show that viral infection did not impede TLR3 responses to poly(I:C). We conclude that NS1 proteins from distinct mosquito-borne flaviviruses do not inhibit TLR3 signaling.
DOI : 10.1016/j.virol.2010.05.020
Anahtar Kelimeler : Flavivirus, West Nile virus, TLR3, Innate immunity, NS1, Interferon
ISSN: 0042-6822
Sayı: 2
Cilt: 404
Sayfa: 319-330
Sınıflandırma :
Biyotıp %100
Viroloji %100
23
Live Zika virus chimeric vaccine candidate based on a yellow fever 17-D attenuated backbone.
Dergi Makalesi

Dergi Makalesi
Open Access
Kaynak :
PubMed
Dergi:
Emerg Microbes Infect
Yazar(lar) :
Franck Touret , Magali Gilles , Raphaelle Klitting , Fabien Aubry , Xavier de Lamballerie , Antoine Nougairède
Yıl :
2018
Xavier de Lamballerie
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 Piri Tam Metin Bulucu
Kaydet Alıntıla
Zika virus (ZIKV) has recently become dispersed throughout the tropics and sub-tropics, causing epidemics associated with congenital disease and neurological complications. There is currently no commercial vaccine for ZIKV. In this study, we describe the initial development of a chimeric virus containing the prM/E proteins of a ZIKV epidemic strain incorporated into a yellow fever 17-D attenuated backbone. Using the versatile and rapid ISA (Infectious Subgenomic Amplicons) reverse genetics method, we compared different constructs and confirmed the need to modify the cleavage site between the pre-peptide and prM protein. Genotypic characterization of the chimeras indicated that the emergence of compensatory mutations in the E protein was required to restore viral replicative fitness. Using an immunocompromised mouse model, we demonstrated that mice infected with the chimeric virus produced levels of neutralizing antibodies that were close to those observed following infection with ZIKV. Furthermore, pre-immunized mice were protected against viscerotropic and neuroinvasive disease following challenge with a heterologous ZIKV strain. These data provide a sound basis for the future development of this ZIKV vaccine candidate.
DOI : 10.1038/s41426-018-0161-7
Sınıflandırma :
Biyotıp %100
Viroloji %100
24
Concurrent Dengue and Malaria
Dergi Makalesi

Dergi Makalesi
Open Access
Kaynak :
Directory of Open Access Journals (DOAJ)
Dergi:
Emerging Infectious Diseases
Yazar(lar) :
Remi N. Charrel , Philippe Brouqui , Cedric Foucault , Xavier de Lamballerie
Yıl :
2005
Xavier de Lamballerie
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DOI : 10.3201/eid1107.041352
Anahtar Kelimeler : Keywords: dengue, malaria, concurrent infection, Guinea, Senegal, Sierra Leone
Cilt: 11
Sayı: 7
Sayfa: 1153 - 1154
Sınıflandırma :
25
Reverse Genetics of RNA Viruses: ISA-Based Approach to Control Viral Population Diversity without Modifying Virus Phenotype
Dergi Makalesi

Dergi Makalesi
Open Access
Kaynak 1:
MDPI
Kaynak 2:
Directory of Open Access Journals (DOAJ)
Dergi:
Viruses
Yayıncı:
MDPI AG
Yazar(lar) :
Jean-Sélim Driouich , Gregory Moureau , Xavier de Lamballerie , Antoine Nougairède
Yıl :
2019
Xavier de Lamballerie
Tam Metin
Kaydet Alıntıla
Reverse genetic systems are essential for the study of RNA viruses. Infectious clones remain the most widely used systems to manipulate viral genomes. Recently, a new PCR-based method called ISA (infectious subgenomic amplicons) has been developed. This approach has resulted in greater genetic diversity of the viral populations than that observed using infectious clone technology. However, for some studies, generation of clonal viral populations is necessary. In this study, we used the tick-borne encephalitis virus as model to demonstrate that utilization of a very high-fidelity, DNA-dependent DNA polymerase during the PCR step of the ISA procedure gives the possibility to reduce the genetic diversity of viral populations. We also concluded that the fidelity of the polymerase is not the only factor influencing this diversity. Studying the impact of genotype modification on virus phenotype is a crucial step for the development of reverse genetic methods. Here, we also demonstrated that the utilization of different PCR polymerases did not affect the phenotype (replicative fitness in cellulo and virulence in vivo) compared to the initial ISA procedure and the use of an infectious clone. In conclusion, we provide here an approach to control the genetic diversity of RNA viruses without modifying their phenotype.
DOI : 10.3390/v11070666
ISSN: 1999-4915
Sayı: 7
Cilt: 11
Sayfa: 666
Sınıflandırma :
Viroloji %99
26
An approach based on RFLP assay to investigate outbreaks of enteroviral meningitis
Dergi Makalesi

Dergi Makalesi
Kaynak :
Science Direct
Dergi:
Journal of Clinical Virology
Yayıncı:
Elsevier
Yazar(lar) :
Rémi N. Charrel , Emmanuelle Bernit , Christine Zandotti , Xavier de Lamballerie
Yıl :
2004
Xavier de Lamballerie
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Background: Enteroviruses (EV) circulate worldwide and are a major cause for annual epidemics of meningitis in humans. During the last two decades, echovirus type 30 (E-30) has revealed to be one of the most prevalent enteroviruses at the origin of epidemics of EV meningitis. : To design an approach to timely investigate epidemics due to EV. To apply this strategy to the outbreak of meningitis due to E-30 that occurred at the end of year 2000 in Marseilles, France. Study design: The approach consisted to (i) determine whether the epidemic was caused by a dominant strain; (ii) identify the dominant strain by sequencing the first isolates during the outbreak; (iii) identify a restriction enzyme, capable to produce an Restriction fragment length polymorphism (RFLP) profile characteristic for the dominant strain, for rapid identification based on RFLP analysis of PCR products. Results and conclusions: A total of 394 samples were tested; 258 (corresponding to 177 patients) were positive for the presence of EV by cell culture and/or RT-PCR. Sequence analysis of a 785-bp PCR product (including the 5′ end of the VP1 gene) performed for the 30 first isolates permitted to identify a RFLP profile that was specific of the dominant strain after enzymatic cleavage by Bst X1. This RFLP profile was observed in 246 out of 258 PCR products. This method of typing is rapid, simple and inexpensive, and may be applied for the epidemiological typing of EV in epidemic situations.
DOI : 10.1016/s1386-6532(03)00089-1
Anahtar Kelimeler : Enterovirus, Picornaviridae, Echovirus, Outbreak, RFLP, PCR
ISSN: 1386-6532
Sayı: 1
Cilt: 29
Sayfa: 54-58
Sınıflandırma :
Viroloji %99
27
Role of host cell factors in flavivirus infection: Implications for pathogenesis and development of antiviral drugs
Dergi Makalesi

Dergi Makalesi
Kaynak :
Science Direct
Dergi:
Antiviral Research
Yayıncı:
Elsevier
Yazar(lar) :
Boris Pastorino , Antoine Nougairède , Nathalie Wurtz , Ernest Gould , Xavier de Lamballerie
Yıl :
2010
Xavier de Lamballerie
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The genus Flavivirus contains approximately 70 arthropod-borne enveloped RNA viruses many of which cause severe human and in some cases, animal disease. They include dengue virus, yellow fever virus, West Nile virus, Japanese encephalitis virus, and tick-borne encephalitis virus. Hundreds of thousands of deaths due to flavivirus infections occur each year, many of which are unpreventable due to lack of availability of appropriate vaccines and/or antiviral drugs. Flaviviruses exploit the cytoplasmic cellular machinery to facilitate propagation of infectious progeny virions. They engage in dynamic and antagonistic interactions with host cell membranes and biochemical processes. Following infection, the cells initiate various antiviral strategies to counteract viral invasion. In its defense, the virus has alternative strategies to suppress these host responses to infection. The fine balance between these interactions determines the outcome of the viral infection and disease progression. Published studies have revealed specific effects of flaviviruses on cellular processes, but the underlying mechanisms that determine the specific cytopathogenetic changes induced by different flaviviruses have not, as yet, been elucidated. Independently of the suppression of the type I IFN response which has been described in detail elsewhere, this review focuses on recent discoveries relating to alterations of host metabolism following viral infection. Such studies may contribute to new approaches to antiviral drug development. The role of host cellular factors will be examined in the context of protection and/or pathogenesis resulting from flavivirus infection, with particular emphasis on West Nile virus and dengue virus.
DOI : 10.1016/j.antiviral.2010.04.014
Anahtar Kelimeler : Flavivirus infection, Host cell factors, Antiviral therapy
ISSN: 0166-3542
Sayı: 3
Cilt: 87
Sayfa: 281-294
Sınıflandırma :
Viroloji %92

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