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Impaired autophagy contributes to adverse cardiac remodeling in acute myocardial infarction.
Dergi Makalesi

Dergi Makalesi
Open Access
Kaynak 1:
Directory of Open Access Journals (DOAJ)
Kaynak 2:
PLOS - Public Library of Science
Dergi:
PLoS ONE
Yazar(lar) :
Xiaoqian Wu , Lishan He , Fajiang Chen , Xiaoen He , Yi Cai , Guiping Zhang , Quan Yi , Meixiang He , Jiandong Luo
Yıl :
2014
Xiaoen He
Tam Metin
Kaydet Alıntıla
OBJECTIVE: Autophagy is activated in ischemic heart diseases, but its dynamics and functional roles remain unclear and controversial. In this study, we investigated the dynamics and role of autophagy and the mechanism(s), if any, during postinfarction cardiac remodeling. METHODS AND RESULTS: Acute myocardial infarction (AMI) was induced by ligating left anterior descending (LAD) coronary artery. Autophagy was found to be induced sharply 12-24 hours after surgery by testing LC3 modification and Electron microscopy. P62 degradation in the infarct border zone was increased from day 0.5 to day 3, and however, decreased from day 5 until day 21 after LAD ligation. These results indicated that autophagy was induced in the acute phase of AMI, and however, impaired in the latter phase of AMI. To investigate the significance of the impaired autophagy in the latter phase of AMI, we treated the mice with Rapamycin (an autophagy enhancer, 2.0 mg/kg/day) or 3-methyladenine (3MA, an autophagy inhibitor, 15 mg/kg/day) one day after LAD ligation until the end of experiment. The results showed that Rapamycin attenuated, while 3MA exacerbated, postinfarction cardiac remodeling and dysfunction respectively. In addition, Rapamycin protected the H9C2 cells against oxygen glucose deprivation in vitro. Specifically, we found that Rapamycin attenuated NFκB activation after LAD ligation. And the inflammatory response in the acute stage of AMI was significantly restrained with Rapamycin treatment. In vitro, inhibition of NFκB restored autophagy in a negative reflex. CONCLUSION: Sustained myocardial ischemia impairs cardiomyocyte autophagy, which is an essential mechanism that protects against adverse cardiac remodeling. Augmenting autophagy could be a therapeutic strategy for acute myocardial infarction.
DOI : 10.1371/journal.pone.0112891
ISSN: 1932-6203
Cilt: 9
Sayı: 11
Sayfa: e112891
Sınıflandırma :
2
SARS-CoV-2: characteristics and current advances in research
Dergi Makalesi

Dergi Makalesi
Open Access
Kaynak :
Directory of Open Access Journals (DOAJ)
Dergi:
Virology Journal
Yazar(lar) :
Yicheng Yang , Zhiqiang Xiao , Kaiyan Ye , Xiaoen He , Bo Sun , Zhiran Qin , Jianghai Yu , Jinxiu Yao , Qinghua Wu , Zhang Bao , Wei Zhao
Yıl :
2020
Xiaoen He
Yayıncı Web Sitesi
 Piri Tam Metin Bulucu
Kaydet Alıntıla
Abstract Coronavirus disease 2019 (COVID-19) caused by SARS-CoV-2 infection has spread rapidly across the world and become an international public health emergency. Both SARS-CoV-2 and SARS-CoV belong to subfamily Coronavirinae in the family Coronaviridae of the order Nidovirales and they are classified as the SARS-like species while belong to different cluster. Besides, viral structure, epidemiology characteristics and pathological characteristics are also different. We present a comprehensive survey of the latest coronavirus—SARS-CoV-2—from investigating its origin and evolution alongside SARS-CoV. Meanwhile, pathogenesis, cardiovascular disease in COVID-19 patients, myocardial injury and venous thromboembolism induced by SARS-CoV-2 as well as the treatment methods are summarized in this review.
DOI : 10.1186/s12985-020-01369-z
Anahtar Kelimeler : SARS-CoV-2, COVID-19, Cytokine storm, Cardiovascular disease, Myocardial injury, Venous thromboembolism
ISSN: 1743-422X
Cilt: 17
Sayı: 1
Sayfa: 1 - 17
Sınıflandırma :
3
Micro-droplet Digital Polymerase Chain Reaction and Real-Time Quantitative Polymerase Chain Reaction Technologies Provide Highly Sensitive and Accurate Detection of Zika Virus.
Dergi Makalesi

Dergi Makalesi
Open Access
Kaynak 1:
PubMed
Kaynak 2:
Springer Link
Dergi:
Virol Sin
Yazar(lar) :
Yuan Hui , Zhiming Wu , Zhiran Qin , Li Zhu , Junhe Liang , Xujuan Li , Hanmin Fu , Shiyu Feng , Jianhai Yu , Xiaoen He , Weizhi Lu , Weiwei Xiao , Qinghua Wu , Bao Zhang , Wei Zhao
Konu :
Humans, Real-Time Polymerase Chain Reaction, Reverse Transcriptase Polymerase Chain Reaction, Zika Virus
Yıl :
2018
Xiaoen He
Piri Tam Metin Bulucu
Kaydet Alıntıla
The establishment of highly sensitive diagnostic methods is critical in the early diagnosis and control of Zika virus (ZIKV) and in preventing serious neurological complications of ZIKV infection. In this study, we established micro-droplet digital polymerase chain reaction (ddPCR) and real-time quantitative PCR (RT-qPCR) protocols for the detection of ZIKV based on the amplification of the NS5 gene. For the ZIKV standard plasmid, the RT-qPCR results showed that the cycle threshold (Ct) value was linear from 101 to 108 copy/μL, with a standard curve R2 of 0.999 and amplification efficiency of 92.203%; however, a concentration as low as 1 copy/μL could not be detected. In comparison with RT-qPCR, the ddPCR method resulted in a linear range of 101-104 copy/μL and was able to detect concentrations as low as 1 copy/μL. Thus, for detecting ZIKV from clinical samples, RT-qPCR is a better choice for high-concentration samples (above 101 copy/μL), while ddPCR has excellent accuracy and sensitivity for low-concentration samples. These results indicate that the ddPCR method should be of considerable use in the early diagnosis, laboratory study, and monitoring of ZIKV.
DOI : 10.1007/s12250-018-0037-y
Anahtar Kelimeler : Micro-droplet digital polymerase chain reaction (ddPCR), Nucleic acid detection, Real-time fluorescence quantitative polymerase chain reaction (RT-qPCR), Zika virus
Sınıflandırma :
Biyotıp %100
Viroloji %100
4
The Antiviral and Antitumor Effects of Defective Interfering Particles/Genomes and Their Mechanisms
Dergi Makalesi

Dergi Makalesi
Open Access
Kaynak :
Directory of Open Access Journals (DOAJ)
Dergi:
Frontiers in Microbiology
Yazar(lar) :
Yicheng Yang , Yicheng Yang , Taibiao Lyu , Runing Zhou , Xiaoen He , Kaiyan Ye , Qian Xie , Li Zhu , Tingting Chen , Chu Shen , Qinghua Wu , Bao Zhang , Wei Zhao
Yıl :
2019
Xiaoen He
Yayıncı Web Sitesi
 Piri Tam Metin Bulucu
Kaydet Alıntıla
Defective interfering particles (DIPs), derived naturally from viral particles, are not able to replicate on their own. Several studies indicate that DIPs exert antiviral effects via multiple mechanisms. DIPs are able to activate immune responses and suppress virus replication cycles, such as competing for viral replication products, impeding the packaging, release and invasion of viruses. Other studies show that DIPs can be used as a vaccine against viral infection. Moreover, DIPs/DI genomes display antitumor effects by inducing tumor cell apoptosis and promoting dendritic cell maturation. With genetic modified techniques, it is possible to improve its safety against both viruses and tumors. In this review, a comprehensive discussion on the effects exerted by DIPs is provided. We further highlight the clinical significance of DIPs and propose that DIPs can open up a new platform for antiviral and antitumor therapies.
DOI : 10.3389/fmicb.2019.01852
Anahtar Kelimeler : defective interfering particles, defective interfering genomes, interference mechanisms, antiviral effect, antitumor effect
ISSN: 1664-302X
Cilt: 10
Sınıflandırma :
Biyotıp %100
Viroloji %97
5
High-specificity targets in SARS-CoV-2 N protein for serological detection and distinction from SARS-CoV
Dergi Makalesi

Dergi Makalesi
Kaynak :
Science Direct
Dergi:
Computers in Biology and Medicine
Yayıncı:
Elsevier BV
Yazar(lar) :
Jianhai Yu , Zhiran Qin , Xuling Liu , Xiaoen He , Jinxiu Yao , Xuan Zhou , Kun Wen , Nan Yu , Qinghua Wu , Weiwei Xiao , Li Zhu , Chengsong Wan , Bao Zhang , Wei Zhao
Yıl :
2022
Xiaoen He
Yayıncı Web Sitesi
Tam Metin
Kaydet Alıntıla
Numerous serological detection kits are being rapidly developed and approved for screening and diagnosing suspected coronavirus disease 2019 (COVID-19) cases. However, cross-reactivity between pre-existing antibodies against other coronaviruses and the captured antigens in these kits can affect detection accuracy, emphasizing the necessity for identifying highly specific antigen fragments for antibody detection. Thus, we performed a conservation and specificity analysis of the novel severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) nucleocapsid (N) protein. We also integrated various B-cell epitope prediction methods to obtain possible dominant epitope regions for the N protein, analyzed the differences in serological antibody levels for different epitopes using ELISA, and identified N protein epitopes for IgG and IgM with high-specificity. The SARS-CoV-2 N protein showed low mutation rates and shared the highest amino acid similarity with SARS-CoV; however, it differed substantially from other coronaviruses. Tests targeting the SARS-CoV-2 N protein produce strong positive results in patients recovering from SARS-CoV. The N18–39 and N183-197 epitopes for IgG and IgM detection, respectively, can effectively overcome cross-reactivity, and even exhibit good specificity between SARS-CoV-2 and SARS-CoV. The antibody levels detected with these were consistent with those detected using the complete N protein. These findings provide a basis for serological diagnosis and determining the kinetics of SARS-CoV-2 antibody detection in patients.
DOI : 10.1016/j.compbiomed.2022.105272
Anahtar Kelimeler : Novel coronavirus (SARS-CoV-2), Serological detection, N protein, Cross-reactivity, Epitope
ISSN: 0010-4825
Cilt: 143
Sayfa: 105272
6
Research advancements in the neurological presentation of flaviviruses
Dergi Makalesi

Dergi Makalesi
Kaynak :
Wiley Online Library
Dergi:
Reviews in Medical Virology
Yazar(lar) :
Tingting Chen , Xiaoen He , Peiru Zhang , Yawen Yuan , Xinyue Lang , Jianhai Yu , Zhiran Qin , Xujuan Li , Qiwei Zhang , Li Zhu , Bao Zhang , Qinghua Wu , Wei Zhao
Konu :
Virology, Infectious Diseases
Yıl :
2018
Xiaoen He
Yayıncı Web Sitesi
Tam Metin
Kaydet Alıntıla
Owing to the large‐scale epidemic of Zika virus disease and its association with microcephaly, properties that allow flaviviruses to cause nervous system diseases are an important area of investigation. At present, although potential pathogenic mechanisms of flaviviruses in the nervous system have been examined, they have not been completely elucidated. In this paper, we review the possible mechanisms of blood‐brain barrier penetration, the pathological effects on neurons, and the association between virus mutations and neurotoxicity. A hypothesis on neurotoxicity caused by the Zika virus is presented. Clarifying the mechanisms of virulence of flaviviruses will be helpful in finding better antiviral drugs and optimizing the treatment of symptoms.
DOI : 10.1002/rmv.2021
ISSN: 1052-9276
Sayı: 1
Cilt: 29

7
Photodynamic inactivation of Shigella flexneri by curcumin
Dergi Makalesi

Dergi Makalesi
Kaynak :
Science Direct
Yazar(lar) :
Zuxin Liang , Xuling Liu , Zhiran Qin , Jingshu Li , Jianhai Yu , Xiaoen He , Li Zhu , Hongying Fan , Yushan Hu , Jinxiu Yao , Chenguang Shen , Chengsong Wan , Bao Zhang , Wei Zhao
Yıl :
2022
Xiaoen He
Yayıncı Web Sitesi
Tam Metin
Kaydet Alıntıla
This study aimed to inactivate Shigella flexneri using curcumin-based photosensitization and determine the sensitivity of S. flexneri to the combination of epsilon (ε)-polylysine (ε-PL) and curcumin-based photosensitization for decontamination. Bacterial numbers were reduced by 4.65 log CFU/mL after incubation with 80 μM curcumin and illumination for 20 min. Photosensitization resulted in cell morphology changes and increased cell permeability and protein degradation. Oxidative stress gene (oxyR) and efflux pump genes (marA, acrA) were upregulated. Decontamination of S. flexneri after incubation with curcumin and ε-PL for 1 h followed by photoactivation for 60 s has been investigated. It was found that the growth of bacteria was completely inhibited. Additionally, the number of bacteria was reduced by > 6 log CFU/mL. Experiments on the uptake of curcumin molecules by bacteria showed that ε-PL promoted the penetration of bacterial cells by curcumin, thus enhancing the effects of photosensitization.
DOI : 10.1016/j.lwt.2021.112491
Anahtar Kelimeler : Photosensitization, Curcumin, Shigella flexneri, Epsilon (ε)-polylysine
ISSN: 0023-6438
Cilt: 153
Sayfa: 112491
8
Effective Suckling C57BL/6, Kunming, and BALB/c Mouse Models with Remarkable Neurological Manifestation for Zika Virus Infection
Dergi Makalesi

Dergi Makalesi
Open Access
Kaynak :
MDPI
Dergi:
Viruses
Yayıncı:
MDPI AG
Yazar(lar) :
Jianhai Yu , Xuling Liu , Changwen Ke , Qinghua Wu , Weizhi Lu , Zhiran Qin , Xiaoen He , Yujing Liu , Jieli Deng , Suiqi Xu , Ying Li , Li Zhu , Chengsong Wan , Qiwei Zhang , Weiwei Xiao , Qian Xie , Bao Zhang , Wei Zhao
Yıl :
2017
Xiaoen He
Yayıncı Web Sitesi
Tam Metin
Kaydet Alıntıla
DOI : 10.3390/v9070165
ISSN: 1999-4915
Sayı: 7
Cilt: 9
Sayfa: 165
Sınıflandırma :
Biyotıp %100
Viroloji %91
9
The effects of Japanese encephalitis virus antibodies on Zika virus infection
Dergi Makalesi

Dergi Makalesi
Kaynak :
Springer Link
Dergi:
Medical Microbiology and Immunology
Yazar(lar) :
Xiaoen He , Xinyue Lang , Jianhai Yu , Li Zhu , Zhiran Qin , Xuling Liu , Pei Chen , Chengguqiu Dai , Tingting Chen , Xujuan Li , Yangyang Chen , Dongrui Zhou , Wanyi Fang , Weiwei Xiao , Bao Zhang , Qian Xie , Qinghua Wu , Wei Zhao
Konu :
Microbiology (medical), Immunology, General Medicine, Immunology and Allergy
Yıl :
2020
Xiaoen He
Yayıncı Web Sitesi
Tam Metin
Kaydet Alıntıla
Recently, Zika virus (ZIKV) has become more widespread, thus attracting global attention. The vaccine against Japanese encephalitis virus (JEV) is currently used in China, being included in planned immunisation regimes. Although ZIKV and JEV are closely related mosquito-borne Flaviviruses, and a complex cross-immune response within flaviviruses has been demonstrated, the effect of JEV vaccination on ZIKV infection has not been well described. Thus, this study aimed to explore the impact of different titres of anti-JEV antibodies (Abs) against ZIKV infection using sera from healthy human donors in Guangzhou and anti-JEV rabbit polyclonal antibodies (pAbs) in vitro and vivo. Human anti-JEV Ab titres were tested at decreasing concentrations as the age increased. A neutralising effect on ZIKV infection was observed when anti-JEV Ab titres in human sera or rabbit pAbs were high (the corresponding age was under 30 years). Even though a lower titre in human sera showed no apparent effect, whereas rabbit pAbs had an antibody-dependent enhancement(ADE)effect, we proved an ADE effect in vivo for the first time. This study suggests that individuals over 60 years of age are at high risk for JEV and ZIKV infection, and screening this age group for infection should strengthen. Furthermore, a deep exploration of the relationship between anti-JEV Abs and ZIKV infection is needed.
DOI : 10.1007/s00430-020-00658-2
Anahtar Kelimeler : Zika virus, Japanese encephalitis virus antibody, Antibody-dependent enhancement(ADE), Neutralisation
ISSN: 0300-8584 1432-1831
Sayı: 2
Cilt: 209
Sayfa: 177-188

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