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1
Numerical study of the formation and drying kinetics of a capillary bridge of trehalose solution between two parallel hydrophilic fibres
Dergi Makalesi

Dergi Makalesi
Kaynak :
Science Direct
Dergi:
Chemical Engineering Science
Yayıncı:
Elsevier
Yazar(lar) :
Yejiong Yu , Aidong Yang , Hua Ye , Julian F Dye , Zhanfeng Cui
Yıl :
2020
Yejiong Yu
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Kaydet
DOI : 10.1016/j.ces.2020.115849
ISSN: 0009-2509
Cilt: 226
Sayfa: 115849
Sınıflandırma :
Kimya %100
Biyoteknoloji %51
2
RT‐LAMP for rapid diagnosis of coronavirus SARS‐CoV‐2
Dergi Makalesi

Dergi Makalesi
Open Access
Kaynak 1:
Directory of Open Access Journals (DOAJ)
Kaynak 2:
Wiley Online Library
Dergi:
Microbial Biotechnology
Yazar(lar) :
Wei E. Huang , Boon Lim , Chiau2010Chen Hsu , Dan Xiong , Wei Wu , Yejiong Yu , Huidong Jia , Yun Wang , Yida Zeng , Mengmeng Ji , Hong Chang , Xiuming Zhang , Hui Wang , Zhanfeng Cui
Yıl :
2020
Yejiong Yu
Piri Tam Metin Bulucu
Kaydet
Summary The pandemic coronavirus SARS‐CoV‐2 in the world has caused a large infected population suffering from COVID‐19. To curb the spreading of the virus, WHO urgently demanded an extension of screening and testing; thus, a rapid and simple diagnostic method is needed. We applied a reverse transcription‐loop‐mediated isothermal amplification (RT‐LAMP) to achieve the detection of SARS‐CoV‐2 in 30 min. We designed four sets of LAMP primers (6 primers in each set), targeting the viral RNA of SARS‐CoV‐2 in the regions of orf1ab, S gene and N gene. A colorimetric change was used to report the results, which enables the outcome of viral RNA amplification to be read by the naked eye without the need of expensive or dedicated instrument. The sensitivity can be 80 copies of viral RNA per ml in a sample. We validated the RT‐LAMP method in a hospital in China, employing 16 clinic samples with 8 positives and 8 negatives. The testing results are consistent with the conventional RT‐qPCR. In addition, we also show that one‐step process without RNA extraction is feasible to achieve RNA amplification directly from a sample. This rapid, simple and sensitive RT‐LAMP method paves a way for a large screening at public domain and hospitals, particularly regional hospitals and medical centres in rural areas.
DOI : 10.1111/1751-7915.13586
ISSN: 1751-7915
Cilt: 13
Sayı: 4
Sayfa: 950 - 961
Sınıflandırma :
Viroloji %99
3
Clinical validation of optimised RT-LAMP for the diagnosis of SARS-CoV-2 infection
Dergi Makalesi

Dergi Makalesi
Kaynak :
Springer Nature
Dergi:
Scientific Reports
Yayıncı:
Springer Science and Business Media LLC
Yazar(lar) :
Boon Lim , Jeremy Ratcliff , Dorota A. Nawrot , Yejiong Yu , Harshmeena R. Sanghani , Chia-Chen Hsu , Leon Peto , Simon Evans , Susanne H. Hodgson , Aikaterini Skeva , Maria Adam , Maria Panopoulou , Christos E. Zois , Katy Poncin , Sridhar R. Vasudevan , Siqi Dai , Shuai Ren , Hong Chang , Zhanfeng Cui , Peter Simmonds , Wei E. Huang , Monique I. AnderssonBoon Lim , Jeremy Ratcliff , Dorota A. Nawrot , Yejiong Yu , Harshmeena R. Sanghani , Chia-Chen Hsu , Leon Peto , Simon Evans , Susanne H. Hodgson , Aikaterini Skeva , Maria Adam , Maria Panopoulou , Christos E. Zois , Katy Poncin , Sridhar R. Vasudevan , Siqi Dai , Shuai Ren , Hong Chang , Zhanfeng Cui , Peter Simmonds , Wei E. Huang , Monique I. Andersson
Yıl :
2021
Yejiong Yu Yejiong Yu
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AbstractWe have optimised a reverse transcription-loop-mediated isothermal amplification (RT-LAMP) assay for the detection of SARS-CoV-2 from extracted RNA for clinical application. We improved the stability and reliability of the RT-LAMP assay by the addition of a temperature-dependent switch oligonucleotide to reduce self- or off-target amplification. We then developed freeze-dried master mix for single step RT-LAMP reaction, simplifying the operation for end users and improving long-term storage and transportation. The assay can detect as low as 13 copies of SARS-CoV2 RNA per reaction (25-μL). Cross reactivity with other human coronaviruses was not observed. We have applied the new RT-LAMP assay for testing clinical extracted RNA samples extracted from swabs of 72 patients in the UK and 126 samples from Greece and demonstrated the overall sensitivity of 90.2% (95% CI 83.8–94.7%) and specificity of 92.4% (95% CI 83.2–97.5%). Among 115 positive samples which Ct values were less than 34, the RT-LAMP assay was able to detect 110 of them with 95.6% sensitivity. The specificity was 100% when RNA elution used RNase-free water. The outcome of RT-LAMP can be reported by both colorimetric detection and quantifiable fluorescent reading. Objective measures with a digitized reading data flow would allow for the sharing of results for local or national surveillance.
DOI : 10.1038/s41598-021-95607-1
ISSN: 2045-2322
Sayı: 1
Cilt: 11
4
Development of a rapid test kit for SARS-CoV-2: an example of product design
Dergi Makalesi

Dergi Makalesi
Kaynak :
Springer Link
Dergi:
Bio-Design and Manufacturing
Yayıncı:
Springer Science and Business Media LLC
Yazar(lar) :
Zhanfeng Cui , Hong Chang , Hui Wang , Boon Lim , Chia-Chen Hsu , Yejiong Yu , Huidong Jia , Yun Wang , Yida Zeng , Mengmeng Ji , Weizhi Liu , Catriona Inverarity , Wei E. Huang
Yıl :
2020
Yejiong Yu
Yayıncı Web Sitesi
Tam Metin
Kaydet
AbstractWe present an example of applying ‘need-driven’ product design principle to the development of a rapid test kit to detect SARS-COV-2 (COVID-19). The tests are intended for use in the field and, longer term, for home use. They detect whether a subject is currently infected with the virus and is infectious. The urgent need for large numbers of tests in field setting imposes constraints such as short test time and lack of access to specialist equipment, laboratories and skilled technicians to perform the test and interpret results. To meet these needs, an antigen test based on RT-LAMP with colorimetric readout was chosen. Direct use of swab sample with no RNA extraction was explored. After extensive experimental study (reported elsewhere), a rapid test kit has been fabricated to satisfy all design criteria.
DOI : 10.1007/s42242-020-00075-7
ISSN: 2096-5524
Sayı: 2
Cilt: 3
Sayfa: 83-86

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