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1
ANALYSIS OF 6-FREQUENCY SIMULTANEOUS OBSERVATIONS OF 170 COMPACT EXTRAGALACTIC OBJECTS IN 1995 1996: A STATISTICS OF MAIN SPECTRA PARAMETERS
Dergi Makalesi

Dergi Makalesi
Open Access
Kaynak :
Directory of Open Access Journals (DOAJ)
Dergi:
Odessa Astronomical Publications
Yazar(lar) :
Yu. Y. Kovalev
Yıl :
2017
Yu. Y. Kovalev
Yayıncı Web Sitesi
 Piri Tam Metin Bulucu
Kaydet Alıntıla
Results of instantaneous spectra observations, carred out at the RATAN-600 during six sets in 1995-1996, are used. We analyse a behaviour of an effective width of spectra, turnover frequency and maximal flux density.
DOI : 10.18524/1810-4215.1996.9.93678
ISSN: 1810-4215
Cilt: 9
Sayfa: 165 - 166
Sınıflandırma :
2
Novel Tumor Suppressor lncRNA Growth Arrest-Specific 5 (GAS5) In Human Cancer
Dergi Makalesi

Dergi Makalesi
Open Access
Kaynak :
Directory of Open Access Journals (DOAJ)
Dergi:
OncoTargets and Therapy
Yazar(lar) :
Yu Y , Hann SS
Yıl :
2019
Yu Y
Yayıncı Web Sitesi
Kaydet Alıntıla
Yaya Yu, Swei Sunny Hann Laboratory of Tumor Biology, The Second Clinical Collage of Guangzhou University of Chinese Medicine, Guangzhou, Guangdong Province 510120, People’s Republic of ChinaCorrespondence: Swei Sunny HannLaboratory of Tumor Biology, The Second Clinical Collage of Guangzhou University of Chinese Medicine, No. 111, Dade Road, Guangzhou, Guangdong Province 510120, People’s Republic of ChinaTel +86 020-39318472Email hann2012@outlook.comAbstract: Long noncoding RNAs (lncRNAs) play crucial regulatory roles in fundamental biological processes, and deregulations of lncRNAs have been linked to numerous human diseases, especially cancers. Of particular interest in this regard is lncRNA GAS5, which is mainly identified as a tumor suppressor in several cancers. GAS5 was significantly low expressed in multiple cancers and was associated with clinic-pathological characteristics and patient survival, indicating a novel potential diagnostic and prognostic biomarker, and a therapeutic target for cancer. Functionally, GAS5 is involved in cell proliferation, metastasis, invasion, apoptosis, epithelial–mesenchymal transition (EMT), and drug resistance, among others, via multiple molecular mechanisms, such as binding to DNA sequences, forming RNA–DNA triplex complex, triggering or suppressing the expression of genes, binding proteins to form chromatin-modifying complex, which activates or represses gene expression, and acting as miRNA sponge to suppress miRNA expression, leading to regulation of miRNA target genes. This review provides an overview of the current state of knowledge and role of GAS5 in clinical relevance, biological functions and molecular mechanisms underlying the dysregulation of expression and function of GAS5 in cancer. Finally, the potential prospective role as diagnostic and prognostic biomarker and therapeutic target in cancer is discussed.Keywords: GAS5, cancer, tumor suppressor, biomarker, therapeutic target
Anahtar Kelimeler : Cancer, Tumor suppressor, Biomarker, Therapeutic target
ISSN: 1178-6930
Cilt: 12
Sayfa: 8421 - 8436
Sınıflandırma :
3
The role of miRNAs in the diagnosis, chemoresistance, and prognosis of pancreatic ductal adenocarcinoma
Dergi Makalesi

Dergi Makalesi
Open Access
Kaynak :
Directory of Open Access Journals (DOAJ)
Dergi:
Therapeutics and Clinical Risk Management
Yazar(lar) :
Ren L , Yu Y
Yıl :
2018
Yu Y
Yayıncı Web Sitesi
Kaydet Alıntıla
Le Ren, Yue Yu Department of Gastroenterology, Affiliated Provincial Hospital, Anhui Medical University, Hefei, China Abstract: Pancreatic ductal adenocarcinoma (PDAC) remains a very challenging malignancy with late presentation, metastatic potential, chemoresistance, and poor prognosis. Therefore, there is an urgent need for novel diagnostic and prognostic biomarkers. miRNAs are small noncoding RNAs that regulate the expression of multitude number of genes. Aberrant expression of miRNAs has been linked to the development of various malignancies, including PDAC. A series of miRNAs have been defined as holding promise for early diagnostics, as indicators of therapy resistance, and even as markers for prognosis in PDAC patients. In this review, we summarize the current knowledge on the role of miRNAs in diagnosis, chemoresistance, and prognosis in PDAC patients. Keywords: pancreatic ductal adenocarcinoma, miRNA, diagnosis, prognosis, chemoresistance
Anahtar Kelimeler : Pancreatic ductal adenocarcinoma, Micro-RNA, Diagnosis, Prognosis, Chemoresistance
Cilt: 14
Sayfa: 179 - 187
4
Is Body Image Associated With Perseverance? A Study Among People With Normal And Increased Body Weight Letter
Dergi Makalesi

Dergi Makalesi
Open Access
Kaynak :
Directory of Open Access Journals (DOAJ)
Dergi:
Psychology Research and Behavior Management
Yazar(lar) :
Yu Y , Li H , Sun Y
Yıl :
2019
Yu Y
Yayıncı Web Sitesi
Kaydet Alıntıla
Yuan Yu,1 Huihong Li,2 Ying Sun1 1Department of Education, School of Education, Tianjin University, Tianjin, People’s Republic of China; 2Administration Department, Tianjin Experimental Kindergarten, Tianjin, People’s Republic of ChinaCorrespondence: Ying SunDepartment of Education, School of Education, Tianjin University, Tianjin, People’s Republic of ChinaTel +86 22 2740 5948Email sunyingtju@126.com We read with great interest the article by Styk et al.1 In their study, the authors found that persistence is associated with body image which evaluated by subjective body weight assessment. In addition, a positive correlation between the distractor resistance index and body mass index (BMI) was found. We wish to express our opinion on the topic and statistical method in this research. View the original paper by Wojciech Styk and colleagues
ISSN: 1179-1578
Cilt: 12
Sayfa: 1027 - 1028
Sınıflandırma :
5
A two-microRNA signature as a diagnostic and prognostic marker of pancreatic adenocarcinoma
Dergi Makalesi

Dergi Makalesi
Open Access
Kaynak :
Directory of Open Access Journals (DOAJ)
Dergi:
Cancer Management and Research
Yazar(lar) :
Yu Y , Feng X , Cang S
Yıl :
2018
Yu Y
Yayıncı Web Sitesi
Kaydet Alıntıla
Yang Yu,* Xiao Feng,* Shundong Cang Department of Oncology, Henan Province People’s Hospital, Henan University, Zhengzhou, Henan, China *These authors contributed equally to this work Background and aim: Some cancer-specific miRNAs are dysregulated in pancreatic adenocarcinoma (PAAD) and involved in cell autophagy, differentiation, proliferation, migration, invasion, and malignant transformation. The aim of our study was to determine a panel of new diagnostic and prognostic biomarkers for PAAD. Methods: We conducted a comprehensive analysis of global miRNA-expression profiles and corresponding prognosis information of 168 PAAD patients from the Cancer Genome Atlas data set. A total of 16 differentially expressed miRNAs were identified as aberrantly expressed in PAAD, and six of these were evaluated for use as diagnostic markers for PAAD. Next, we confirmed a two-miRNA signature significantly associated with PAAD patient diagnosis and outcome prediction. Results: The panel of two miRNAs showed outstanding diagnostic performance, with sensitivity of 100% and specificity of 87.5%. Finally, we divided the PAAD patients into high-risk and low-risk groups based on the expression profile of the two miRNAs. Kaplan–Meier analysis demonstrated that patients in the high-risk group had significantly worse prognosis than patients in the low-risk group. Univariate and multivariate Cox regression analysis showed that the two-miRNA signature was an independent prognostic factor for the overall survival of PAAD patients. Conclusion: Taken together, the two-miRNA signature may serve as an accurate and sensitive biomarker for diagnosis and PAAD-outcome prediction, facilitating the diagnosis and potentially improving treatment outcome of PAAD. Keywords: pancreatic adenocarcinoma, microRNA signature, TCGA, prognosis, diagnosis
Anahtar Kelimeler : Pancreatic adenocarcinoma, microRNA signature, prognosis, diagnosis
ISSN: 1179-1322
Cilt: 10
Sayfa: 1507 - 1515
Sınıflandırma :
Biyotıp %100
Hematoloji %66
6
Logical optimization of Boolean nets using Shannon expansion
Dergi Makalesi

Dergi Makalesi
Open Access
Kaynak :
Directory of Open Access Journals (DOAJ)
Dergi:
Informatika
Yazar(lar) :
P. N. Bibilo , Yu. Y. Lankevich
Yıl :
2019
Yu. Y. Lankevich
Yayıncı Web Sitesi
Kaydet Alıntıla
A synthesis of logical circuits, comprising functional combination blocks of very large scale integration circuits, is one of the most important tasks of computer-aided design. As the data size of design tasks increases, the execution time of synthesis of logic circuits also increases. The global technological independent optimization as the first stage of synthesis of logical circuit is especially labor-consuming. The second stage is technological mapping of optimized logical representations of functions to the logical elements of technological library. The main features of logical circuit, such as area, performance, power consumption, depend on the efficiency of the first stage – global logical optimization. The evolution of methods of global logical optimization has revealed the efficiency of Shannon expansion in case of optimization of multi-level representations of the systems of fully defined Boolean function. A number of methods and programs were developed using graphical representations of Shannon expansions – BDD representations. Most of the developed methods of optimization of BDD-representations use the initial representations of functions systems in the form of disjunctive normal form (DNF).In the article an algorithm of minimization of nodes number of Boolean net, which is a multi-level representation of the system of fully defined Boolean function, is proposed. Minimization is based on Shannon expansion and a search of equal (with accuracy up to inversion) nodes in Boolean net. Such algorithm of logical optimization was implemented as application. The experiments have shown that this algorithm and the application is reasonable to use in cases when the initial multi-level representation of functions is impossible to define as DNF system, or when DNF system contains a large number of elementary conjunctions.
Anahtar Kelimeler : boolean function, boolean net, shannon expansion, disjunctive normal form, synthesis of logical circuits
ISSN: 1816-0301
Cilt: 16
Sayı: 2
Sayfa: 73 - 89
Sınıflandırma :
7
The emerging roles of PIWI-interacting RNA in human cancers
Dergi Makalesi

Dergi Makalesi
Open Access
Kaynak :
Directory of Open Access Journals (DOAJ)
Dergi:
Cancer Management and Research
Yazar(lar) :
Yu Y , Xiao J , Hann SS
Yıl :
2019
Yu Y
Yayıncı Web Sitesi
Kaydet Alıntıla
Yaya Yu,1 Jing Xiao,2 Swei Sunny Hann1,31Laboratory of Tumor Biology, The Second Clinical College of Guangzhou University of Chinese Medicine, Guangzhou, Guangdong Province, 510120, People’s Republic of China; 2Department of Gynecology, The Second Clinical College of Guangzhou University of Chinese Medicine, Guangzhou, Guangdong Province, 510120, People’s Republic of China; 3Guangdong Provincial Key Laboratory of Clinical Research on Traditional Chinese Medicine Syndrome, The Second Clinical College of Guangzhou University of Chinese Medicine, Guangzhou, Guangdong Province, 510120, People’s Republic of ChinaAbstract: PIWI-interacting RNAs (piRNAs) are a type of non-coding RNAs that interact with PIWI proteins, which are members of the argonaute family. Originally described in the germline, piRNAs are also expressed in human somatic cells in a tissue-specific manner. piRNAs are involved in spermatogenesis, germ stem-cell maintenance, silencing of transposon, epigenetic and genomic regulation and rearrangement. A large number of studies have demonstrated that expression of piRNAs is involved in many kinds of disease, including cancer. Abnormal expression of piRNAs is emerging as a critical player in cancer cell proliferation, apoptosis, invasion, and migration in vitro and in vivo. Functionally, piRNAs maintain genomic integrity by repressing the mobilization of transposable elements, and regulate the expression of downstream target genes via transcriptional or post-transcriptional mechanisms. Furthermore, altered expression of piRNAs in cancer is linked to clinical outcome, highlighting the important role that they may play as novel diagnostic and prognostic biomarkers, and as therapeutic targets for cancer therapy. In this review, we focus on the biogenesis and the functional roles of piRNAs in cancers, discuss emerging insights into the roles of piRNAs in the occurrence, progression, and treatment of cancers, reveal various mechanisms underlying piRNAs-mediated gene regulation, and highlight their potential clinical utilities as biomarkers as well as potential targets for cancer treatment.Keywords: PIWI-interacting RNA, cancer, biogenesis, biomarkers, therapeutics
Anahtar Kelimeler : PIWI-interacting RNA, Cancer, Biogenesis, Biomarkers, Therapeutics
ISSN: 1179-1322
Cilt: 11
Sayfa: 5895 - 5909
Sınıflandırma :
8
Influence of thoracic drainage fluid on proliferation, migration, apoptosis, and drug resistance in lung cancer cell lines
Dergi Makalesi

Dergi Makalesi
Open Access
Kaynak :
Directory of Open Access Journals (DOAJ)
Dergi:
Cancer Management and Research
Yazar(lar) :
Mao Y , Yu Y , Han Y
Yıl :
2019
Yu Y
Yayıncı Web Sitesi
Kaydet Alıntıla
Yuqiang Mao,1 Ying Yu,2 Yun Han1 1Department of Thoracic Surgery, Shengjing Hospital of China Medical University, Shenyang 110004, China; 2Liaoning Medical Device Test Institute, Shenyang 110179, China Background: This study aimed to clarify the effect of thoracic drainage fluid (DF) on lung cancer cells in vitro. Methods: We assessed the influence of DF on the proliferation and migration of lung cancer cells (LTEP-a-2 and A549) using the MTT cell proliferation assay and scratch wound assay. Cell apoptosis was determined by flow cytometric analysis. We also investigated the effect of DF on drug chemosensitivity, assessing viability of LTEP-a-2 and A549 cells. Results: The proliferative rates of cancer cells in the DF-treated group were significantly higher than those of the control group. Similar results were obtained for cell migration of lung cancer cells. Cells in the DF-treated groups showed a lower percentage of apoptosis than those of the control groups. Chemosensitivity of lung cancer cells to doxycycline and cisplatin (DDP) was lowered by DF. Conclusion: These findings suggest that DF affects lung cancer cells by promoting proliferation and migration, inhibiting apoptosis, and increasing drug resistance. Keywords: lung cancer, drainage fluid, proliferation and migration, apoptosis, drug resistance
Anahtar Kelimeler : lung cancer, drainage fluid, proliferation and migration, apoptosis, drug resistance
ISSN: 1179-1322
Cilt: 11
Sayfa: 2253 - 2259
Sınıflandırma :
Biyotıp %100
Biyotıp %67
9
MicroRNA-1246 Promotes Melanoma Progression Through Targeting FOXA2
Dergi Makalesi

Dergi Makalesi
Open Access
Kaynak :
Directory of Open Access Journals (DOAJ)
Dergi:
OncoTargets and Therapy
Yazar(lar) :
Yu Y , Yu F , Sun P
Yıl :
2020
Yu Y
Yayıncı Web Sitesi
Kaydet Alıntıla
Yanhua Yu,1 Fang Yu,1 Pijiang Sun2 1Department of Dermatology, Weihai Central Hospital Affiliated to Qingdao University, Weihai 264400, People’s Republic of China; 2Department of Hepatobiliary and Abdominal Hernias Surgery, Weihai Central Hospital Affiliated to Qingdao University, Weihai 264400, People’s Republic of ChinaCorrespondence: Pijiang SunDepartment of Hepatobiliary and Abdominal Hernias Surgery, Weihai Central Hospital Affiliated to Qingdao University, No. 3 Mishandong Road, Wendeng District, Weihai 264400, Shandong, People’s Republic of ChinaTel +86 152 6441 0981Email beciham@163.comIntroduction: Recently, the incidence of melanoma has been rising and there is a lack of effective targeted therapies. The regulatory mechanisms of microRNA-1246 (miR-1246) have been found in many cancers, except melanoma. This study focused on the regulatory mechanism of miR-1246 in melanoma development.Methods: The expression of miR-1246 was assessed using quantitative real-time polymerase chain reaction (RT-qPCR). Cell viability and metastasis were detected by Transwell and MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) assays. The protein expression of epithelial mesenchymal transition (EMT) makers was assessed by Western blot analysis. The target gene of miR-1246 was detected using luciferase reporter assay.Results: MiR-1246 expression was increased in melanoma tissues and cells. In addition, upregulation of miR-1246 promoted cell viability and metastasis in melanoma. Forkhead box protein A2 (FOXA2) was confirmed to be a direct target of miR-1246. And FOXA2 expression was decreased in melanoma and was suppressed by miR-1246. Importantly, upregulation of FOXA2 restored the carcinogenesis of miR-1246 in melanoma.Conclusion: MiR-1246 promoted cell viability and metastasis in melanoma by inhibiting FOXA2 expression.Keywords: miR-1246, melanoma, cell viability, cell metastasis, FOXA2
Anahtar Kelimeler : mir-1246, melanoma, cell viability, cell metastasis, foxa2
ISSN: 1178-6930
Cilt: 13
Sayfa: 1245 - 1253
Sınıflandırma :
Biyotıp %71
10
Reduction behavior of Tin-containing phase in Tin-bearing iron concentrates under CO-CO2 mixed gases
Dergi Makalesi

Dergi Makalesi
Open Access
Kaynak :
Directory of Open Access Journals (DOAJ)
Dergi:
Journal of Mining and Metallurgy. Section B: Metallurgy
Yazar(lar) :
Yu Y. , Li H.-J. , Li L.
Yıl :
2019
Yu Y.
Yayıncı Web Sitesi
Tam Metin
Kaydet Alıntıla
The main purpose of this study was to ascertain the reduction behavior of tin phase (SnO2) in tin-bearing iron concentrates at the respective temperature of 1273 and 1373 K in diverse CO-CO2 mixed gases using chemical analysis, XRD, and SEMEDS analysis. The results show that the reduction behavior of SnO2 depends on the roasting temperature and CO content. At 1273 K, the SnO2 will be reduced to Sn (l) with the CO content being higher than 17.26 vol%, and there is no formation of SnO(s). With the temperature increased to 1373 K, the SnO2 is reduced stepwise in the order to form SnO2 → SnO (l) → Sn(l) with CO content over 15.75 vol%. The kinetic study shows that activation energy of the reaction SnO2(s)+CO(g)=Sn(l)+ CO2(g) is 144.75 kJ/mol at 1073-1223 K, being far lower than the one in the reduction of SnO2(s) into SnO(g) at 1273-1323 K, which leads to a conclusion that the tin in tin-bearing iron concentrates could be removed effectively after the Sn(l) sulfurated into SnS at relatively lower temperatures (1073-1223 K) using the sulfidation roasting method.
DOI : 10.2298/JMMB181121025Y
Anahtar Kelimeler : reduction behavior, co-co2 mixed gases, kinetics, tin-bearing iron concentrates
Cilt: 55
Sayı: 2
Sayfa: 177 - 185
Sınıflandırma :
11
Study on Physical Account and Value Account of Sea Area Resources
Dergi Makalesi

Dergi Makalesi
Open Access
Kaynak :
Directory of Open Access Journals (DOAJ)
Dergi:
E3S Web of Conferences
Yazar(lar) :
Wang P , Lin X , Yu Y H
Yıl :
2019
Yu Y H
Yayıncı Web Sitesi
Tam Metin
Kaydet Alıntıla
Conducting surveys on the status quo of sea area resources, figuring out the basic information about endowment of sea area resources, constructing an accounting standard system for sea area resource assets - value and establishing a physical account and a value account for sea area resource reserve are the foundation for sea area price evaluation and necessary conditions for the perfection of natural resource asset property rights system. This paper analyzes the survey on the status quo of sea area resources in China and the status quo of resource account and value account, brings up a problem that surveys and accountings of sea area resources cannot meet the management requirements of paid utilization of sea area and offers specific suggestions such as carrying out surveys on the space resources of sea area, evaluating the value of resources.
DOI : 10.1051/e3sconf/20199301001
ISSN: 2267-1242
Cilt: 93
Sayfa: 01001
Sınıflandırma :
12
Surface characterization of cast Ti-6Al-4V in hydrofluoric-nitric pickling solutions
Dergi Makalesi

Dergi Makalesi
Kaynak :
Science Direct
Dergi:
Surface and Coatings Technology
Yayıncı:
Elsevier
Yazar(lar) :
Wen C Say , Yu Y Tsai
Yıl :
2004
Yu Y Tsai
Yayıncı Web Sitesi
Tam Metin
Kaydet Alıntıla
Hydrofluoric acid offers a fast etching rate for removing 200 μm-thick alpha-cases from the surfaces of cast Ti-6Al-4V. The acid picks up hydrogen gas into substrate of the cast, which can be limited by introducing an oxidizing acid. This study proposes the optimal ratios of hydrofluoric/nitric acid as the pickling solutions. The cast Ti-6Al-4V in these solutions is monitored with chemical etching and electrochemical polarization. The mechanisms of etching procedures are initiated by generating oxygen vacancies that lead to several products from a series of quasi-chemical steps. The final product of the reactions may deduce as the form of H2TiF6 that is peeled out by the evolution of produced gases. Polarized curves are performed to illustrate passive behaviors of the alpha-case removed Ti-6Al-4V. The amounts of charge transference increase with increasing hydrofluoric acid concentrations. The newly formed passive film was estimated to be 20 nm in thickness with non-stoichiometric arrangements in the 4% HF electrolyte.
DOI : 10.1016/s0257-8972(03)00747-3
ISSN: 0257-8972
Sayı: 3
Cilt: 176
Sayfa: 337-343
Sınıflandırma :
13
Knockdown of lncRNA XIST Suppresses Cell Tumorigenicity in Human Non-Small Cell Lung Cancer by Regulating miR-142-5p/PAX6 Axis
Dergi Makalesi

Dergi Makalesi
Open Access
Kaynak :
Directory of Open Access Journals (DOAJ)
Dergi:
OncoTargets and Therapy
Yazar(lar) :
Jiang Q , Xing W , Cheng J , Yu Y
Yıl :
2020
Yu Y
Yayıncı Web Sitesi
Kaydet Alıntıla
Qingfeng Jiang, Wenqun Xing, Jinhua Cheng, Yongkui Yu Department of Thoracic Surgery, The Affiliated Cancer Hospital of Zhengzhou University, Henan Cancer Hospital, Zhengzhou, Henan 450008, People’s Republic of ChinaCorrespondence: Wenqun XingDepartment of Thoracic Surgery, The Affiliated Cancer Hospital of Zhengzhou University, Henan Cancer Hospital, No. 127, Dongming Road, Zhengzhou City, Henan Province, People’s Republic of ChinaTel +86- 371-5588265Email dwjkcl@163.comBackground: Long noncoding RNA X inactivate-specific transcript (lncRNA XIST) has been identified to contribute to the development and progression of non-small cell lung cancer (NSCLC). Thus, it is important to explore more specific functions and molecular mechanisms of XIST in NSCLC tumorigenesis.Materials and Methods: The expression of XIST, microRNA (miR)-142-5p and paired box 6 (PAX6) was measured using quantitative real-time polymerase chain reaction or Western blot, respectively. Cell proliferation was analyzed using cell counting kit-8 (CCK-8) assay. Flow cytometry was utilized to measure apoptotic cells. Cell migration and invasion were determined by Transwell assay. The interaction between miR-142-5p and XIST or PAX6 was confirmed by the dual-luciferase reporter assay and RNA immunoprecipitation assay. In vivo experiments were performed through the murine xenograft model.Results: XIST was elevated in NSCLC, and XIST knockdown suppressed cell proliferation, migration, invasion and induced apoptosis in vitro as well as repressed tumor growth in vivo. MiR-142-5p was a target of XIST, and silencing miR-142-5p reversed the anti-tumor functions mediated by XIST knockdown in NSCLC cells. PAX6 was confirmed to be a target of miR-142-5p, and the inhibitory effects caused by miR-142-5p restoration in NSCLC cell malignant phenotypes were attenuated by PAX6 overexpression. Besides that, XIST could indirectly regulate PAX6 expression by sponging miR-142-5p in vivo and in vitro.Conclusion: XIST suppresses cell tumorigenicity in human NSCLC by regulating miR-142-5p/PAX6 axis, which indicates a novel insight into the pathogenesis of NSCLC and lays a foundation for the molecular therapy of NSCLC.Keywords: XIST, miR-142-5p, PAX6, NSCLC
Anahtar Kelimeler : mir-142-5p, nsclc
ISSN: 1178-6930
Cilt: 13
Sayfa: 4919 - 4929
Sınıflandırma :
14
Diagnosis and treatment of synchronous multiple primary lung cancer – a case report
Dergi Makalesi

Dergi Makalesi
Open Access
Kaynak :
Directory of Open Access Journals (DOAJ)
Dergi:
OncoTargets and Therapy
Yazar(lar) :
Bao Y , Wu J , Zhang J , Yu Y
Yıl :
2019
Yu Y
Yayıncı Web Sitesi
Kaydet Alıntıla
Yi Bao,1,2 Jiayuan Wu,1 Jun Zhang,3 Yawei Yu41The Key Laboratory, The Second Affiliated Hospital of Jiaxing University, Jiaxing, Zhejiang, People’s Republic of China; 2The Department of Oncology, The Second Affiliated Hospital of Jiaxing University, Jiaxing, Zhejiang, People’s Republic of China; 3The Department of Thoracic Surgery, The Second Affiliated Hospital of Jiaxing University, Jiaxing, Zhejiang, People’s Republic of China; 4The Department of Pathology, The Second Affiliated Hospital of Jiaxing University, Jiaxing, Zhejiang, People’s Republic of ChinaAbstract: The incidence of synchronous multiple primary lung cancer (SMPLC) has been increased in recent years. Because of a variance in clinical management and outcome, it is important to distinguish SMPLC from a primary tumor with intrapulmonary metastases. Here, we reported a diagnosis and treatment procedure regarding a case of a 58‐year‐old woman who presented with synchronous multiple tumor lesions in separate lungs. Using a next generation sequencing technology, a discordant EGFR gene profile from the separate lungs was revealed for this patient. After standard treatment procedures, the therapeutic effects were evaluated by response evaluation criteria in solid tumors (RECIST). This case shows an essential role in the combination of molecular features, together with pathological analysis, during the management of SMPLC, but challenges still required considering during dealing the cases of SMPLC.Keywords: lung adenocarcinoma, SMPLC, genetic features, EGFR
Anahtar Kelimeler : lung adenocarcinoma, SMPLC, genetic features
ISSN: 1178-6930
Cilt: 12
Sayfa: 4281 - 4285
Sınıflandırma :
15
microRNA-519d Induces Autophagy and Apoptosis of Human Hepatocellular Carcinoma Cells Through Activation of the AMPK Signaling Pathway via Rab10
Dergi Makalesi

Dergi Makalesi
Open Access
Kaynak :
Directory of Open Access Journals (DOAJ)
Dergi:
Cancer Management and Research
Yazar(lar) :
Zhang YJ , Pan Q , Yu Y , Zhong XP
Yıl :
2020
Yu Y
Yayıncı Web Sitesi
Kaydet Alıntıla
Yi-Jie Zhang,1,2 Qi Pan,1,2 Yang Yu,1,2 Xin-Ping Zhong1,2 1Department of Hepatobiliary and Organ Transplantation, The First Affiliated Hospital of China Medical University, Shenyang 110001, People’s Republic of China; 2The Key Laboratory of Organ Transplantation of Liaoning Province, The First Affiliated Hospital of China Medical University, Shenyang 110001, People’s Republic of ChinaCorrespondence: Xin-Ping ZhongDepartment of Hepatobiliary and Organ Transplantation, The First Affiliated Hospital of China Medical University, No. 155, Nanjing North Street, Heping District, Shenyang 110001, Liaoning Province, People’s Republic of ChinaTel +86-13309831481Email zhongxp2@yeah.netBackground and Aim: Hepatocellular carcinoma (HCC) is a type of cancer with high mortality rates. The overexpression of microRNA-519d (miR-519d) has been explored in different types of cancers, which could significantly help suppress cancer development. This study aimed to investigate the interaction of miR-519d with its target gene, Rab10, as well as its effects on cell proliferation and autophagy in HCC cells through modulation of the AMPK signaling pathway.Methods: Microarray analysis was used to analyze the differentially expressed genes in HCC, and the target genes of the screened-out miRNA were predicted and verified. The expression of miR-519d and Rab10, AMPK signaling pathway-related proteins, apoptosis- and autophagy-related proteins was determined by RT-qPCR and Western blot analysis in HCC tissues and cell lines. Lastly, the effects of miR-519d and Rab10 in HCC cell proliferation, apoptosis, and mouse tumour xenograft in vivo were examined through gain- and loss-of-function experiments.Results: MiR-519d was down-regulated and Rab10 was upregulated in HCC tissues and cell lines. Overexpression of miR-519d decreased the expression of Rab10, mTOR, and Bcl-2, but increased the expression of Bax, Beclin1, Atg5, and p53. Upregulated miR-519d and downregulated Rab10 expression suppressed cell proliferation and induced cell apoptosis and autophagy in HCC cells. Finally, upregulation of miR-519d inhibited tumour growth in vivo.Conclusion: The result obtained in this study indicates that up-regulation of miR-519d inhibits proliferation and promotes apoptosis and autophagy of HCC cells through activation of the AMPK signaling pathway via downregulating Rab10, which provides a potential target for the treatment of HCC.Keywords: microRNA-519, Rab10, Adenosine 5ʹ-monophosphate-activated protein signaling pathway, hepatocellular carcinoma, proliferation, autophagy
Anahtar Kelimeler : microrna-519, rab10, adenosine 5u2019-monophosphate-activated protein signaling pathway, hepatocellular carcinoma, proliferation, autophagy
ISSN: 1179-1322
Cilt: 12
Sayfa: 2589 - 2602
Sınıflandırma :
Biyotıp %100
Apoptoz %50
16
Long Noncoding RNA LINC00173 Contributes to the Growth, Invasiveness and Chemo-Resistance of Colorectal Cancer Through Regulating miR-765/PLP2 Axis
Dergi Makalesi

Dergi Makalesi
Open Access
Kaynak :
Directory of Open Access Journals (DOAJ)
Dergi:
Cancer Management and Research
Yazar(lar) :
Yu Y , Lu X , Yang C , Yin F
Yıl :
2020
Yu Y
Yayıncı Web Sitesi
Kaydet Alıntıla
Yanhui Yu,1,* Xiuyun Lu,2,* Chuandong Yang,3 Fengxiang Yin4 1Physical Examination Center, The 2nd Affiliated Hospital of Harbin Medical University, Harbin 150081, People’s Republic of China; 2Department of Traditional Chinese Medicine, The 2nd Affiliated Hospital of Harbin Medical University, Harbin 150081, People’s Republic of China; 3Department of Orthopedics, The 4th Affiliated Hospital of Harbin Medical University, Harbin 150001, People’s Republic of China; 4Department of Integrated Traditional Chinese Medicine and Western Medicine, Harbin Chest Hospital, Harbin 150056, People’s Republic of China*These authors contributed equally to this workCorrespondence: Yanhui YuPhysical Examination Center, The 2nd Affiliated Hospital of Harbin Medical University, Harbin 150081, People’s Republic of ChinaEmail yanhuiyuaaa@sina.comBackground: Long noncoding RNA has been involved in tumorigenesis of colorectal cancer (CRC). This study aimed to illustrate the functions and mechanisms of LINC00173 in CRC progression.Methods: The expression of LINC00173 in CRC tissues and cell lines were analyzed via qRT-PCR. Kaplan–Meier curve was used to determine survival rate. Luciferase reporter assay was conducted to evaluate the interactions among LINC00173, miR-765 and PLP2 (proteolipid protein 2). CCK8 assay, EdU assay, transwell assay and xenograft assay were performed to examine the effect of LINC00173/miR-765/PLP2 axis on proliferation, migration and invasion. The Ki67 expression level in tumors tissues was detected through immunofluorescence assay.Results: LINC00173 expression was markedly upregulated in CRC tissues and cells. High expression level of LINC00173 in CRC patients was correlated with poor prognosis. LINC00173 knockdown inhibited proliferation, migration, invasion and chemo-resistance of CRC cells in vitro. LINC00173 downregulation delayed CRC growth in vivo. LINC00173 interacted with miR-765 to promote PLP2 expression.Conclusion: Our results demonstrated that LINC00173 plays an important oncogenic role in CRC via modulating miR-765/PLP2 axis. And LINC00173 may be a potential prognostic biomarker and therapeutic target for CRC.Keywords: LINC00173, miR-765, PLP2, colorectal cancer
Anahtar Kelimeler : linc00173, mir-765, colorectal cancer
ISSN: 1179-1322
Cilt: 12
Sayfa: 3363 - 3369
Sınıflandırma :
17
Histamine H3 Receptor Promotes Cell Survival via Regulating PKA/CREB/CDKN1A Signal Pathway in Hepatocellular Carcinoma
Dergi Makalesi

Dergi Makalesi
Open Access
Kaynak :
Directory of Open Access Journals (DOAJ)
Dergi:
OncoTargets and Therapy
Yazar(lar) :
Zhang C , Yu Y , Ma L , Fu P
Yıl :
2020
Yu Y
Yayıncı Web Sitesi
Kaydet Alıntıla
Chunle Zhang,1 Yang Yu,2 Liang Ma,1 Ping Fu1,2 1Kidney Research Laboratory, Division of Nephrology and National Clinical Research Center for Geriatrics, West China Hospital of Sichuan University, Chengdu 610041, People’s Republic of China; 2Department of Nephrology, West China Hospital of Sichuan University, Chengdu 610041, People’s Republic of ChinaCorrespondence: Ping FuDepartment of Nephrology, Kidney Research Laboratory, Division of Nephrology and National Clinical Research Center for Geriatrics, West China Hospital of Sichuan University, Chengdu 610041, People’s Republic of ChinaTel/ Fax +86-028-85422286Email fupinghx@163.comBackground: The histamine H3 receptor (HRH3) is mainly expressed in areas of the brain involved in the regulation of the release of various neurotransmitters. Recent studies have shown that HRH3 expression is increased in several types of carcinomas. However, the functional roles and underlying molecular mechanism by which HRH3 regulates cell survival in hepatocellular carcinoma (HCC) remain unknown.Methods: The mRNA and protein expression level of target genes were evaluated by qRT-PCR, Western blot and immunohistochemistry, respectively. Cell viability and cell proliferation activity were assessed by MTS assay and EdU incorporation assay. Cell apoptosis and cell cycle were assessed by flow cytometry analysis. A xenograft mouse model was constructed to investigate the effect of HRH3 on tumor growth in vivo.Results: Our results indicated that HRH3 was significantly upregulated in HCC, which promoted cell survival by accelerating cell proliferation and inhibiting cell apoptosis. Our results also showed that HRH3 in HCC downregulated the expression of cyclin-dependent kinase inhibitor p21 (CDKN1A) to promote G1-S phase transition by inactivating the cAMP/PKA/CREB pathway, which finally contributed to the malignant growth of HCC.Conclusion: Our findings indicated that HRH3 functioned in promoting HCC survival by inactivating the cAMP/PKA/CREB pathway to downregulate CDKN1A expression. Thus, HRH3 might serve as a potential therapeutic target in HCC treatment.Keywords: HRH3, CDKN1A, cell survival, hepatocellular carcinoma
Anahtar Kelimeler : cdkn1a, cell survival, hepatocellular carcinoma
ISSN: 1178-6930
Cilt: 13
Sayfa: 3765 - 3776
Sınıflandırma :
18
Long Non-Coding RNA TP73-AS1 Promotes the Development of Lung Cancer by Targeting the miR-27b-3p/LAPTM4B Axis
Dergi Makalesi

Dergi Makalesi
Open Access
Kaynak :
Directory of Open Access Journals (DOAJ)
Dergi:
OncoTargets and Therapy
Yazar(lar) :
Jiang Q , Xing W , Cheng J , Yu Y
Yıl :
2020
Yu Y
Yayıncı Web Sitesi
Kaydet Alıntıla
Qingfeng Jiang, Wenqun Xing, Jinhua Cheng, Yongkui Yu Department of Thoracic Surgery, The Affiliated Cancer Hospital of Zhengzhou University, Henan Cancer Hospital, Zhengzhou, Henan, People’s Republic of ChinaCorrespondence: Wenqun XingDepartment of Thoracic Surgery, The Affiliated Cancer Hospital of Zhengzhou University, Henan Cancer Hospital, No. 127 Dongming Road, Zhengzhou 450008, Henan, People’s Republic of ChinaTel +86-0371-65588265Email kcbbgn@163.comPurpose: Long non-coding RNA P73 antisense RNA 1T (TP73-AS1) is a newly discovered lncRNA involved in the occurrence and development of several cancers. However, its role in lung cancer has not been well investigated yet.Methods: The expressions of TP73-AS1, microRNA-27b-3p (miR-27b-3p) and lysosomal-associated protein transmembrane-4 Beta (LAPTM4B) were detected by quantitative real-time polymerase chain reaction (qRT-PCR). The cell proliferation, apoptosis, migration and invasion were detected by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), Annexin V-FITC/PI and transwell assays, respectively. Tumor xenografts were applied to explore the role of TP73-AS1 in vivo. The target relationship was predicted by StarBase v.2.0 or TargetScan and confirmed by luciferase reporter assay. Pearson’s coefficient assay was applied to assess the expression correlation between two groups. Protein expression levels were detected by Western blot.Results: We found that TP73-AS1 was strikingly up-regulated in lung cancer tissues and cells. TP73-AS1 depletion inhibited the growth and metastasis of lung cancer cells in vitro. Furthermore, TP73-AS1 could act as an endogenous sponge by directly binding miR-27b-3p, and a notable inverse correlation between them was also discovered. Importantly, knockdown of miR-27b-3p could reverse the inhibitory effects of TP73-AS1 depletion on the growth and metastasis of lung cancer cells. Besides, LAPTM4B was directly targeted by miR-27b-3p and could be co-regulated by TP73-AS1 and miR-27b-3p in lung cancer cells. Silencing TP73-AS1 hampered tumor growth by regulating miR-27b-3p/LAPTM4B axis in vivo.Conclusion: TP73-AS1 promoted the progression of lung cancer through regulating miR-27b-3p/LAPTM4B axis and it might be a potential target for diagnosis and treatment of lung cancer.Keywords: lung cancer, TP73-AS1, miR-27b-3p, LAPTM4B, proliferation, metastasis
Anahtar Kelimeler : lung cancer, tp73-as1, mir-27b-3p, laptm4b, proliferation, metastasis
ISSN: 1178-6930
Cilt: 13
Sayfa: 7019 - 7031
Sınıflandırma :
19
Comparison of dexmedetomidine and sufentanil as adjuvants to local anesthetic for epidural labor analgesia: a randomized controlled trial
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Dergi Makalesi
Open Access
Kaynak :
Directory of Open Access Journals (DOAJ)
Dergi:
Drug Design, Development and Therapy
Yazar(lar) :
Zhang T , Yu Y , Zhang W , Zhu J
Yıl :
2019
Yu Y
Yayıncı Web Sitesi
Kaydet Alıntıla
Tao Zhang,1 Yulong Yu,1 Wang Zhang,2,* Jin Zhu3,* 1Department of Anesthesiology, Taizhou Hospital of Zhejiang Province, Wenzhou Medical University, Linhai 317000, People’s Republic of China; 2Department of Anesthesiology, Affiliated Women’s and Children’s Hospital of Jiaxing University, Jiaxing 314000, People’s Republic of China; 3Department of Surgical Oncology, Taizhou Municipal Hospital, Taizhou 318000, People’s Republic of China*These authors contributed equally to this work Background: The epidural dexmedetomidine combined with ropivacaine has been successfully used for labor analgesia. We compared the effects of dexmedetomidine and sufentanil as adjuvants to local anesthetic for epidural labor analgesia.Methods: Eighty nulliparous women were enrolled in the double-blind study and randomly divided into two groups. Group D received 0.5 µg/mL dexmedetomidine with 0.1% ropivacaine for epidural labor analgesia, and group S (control group) received 0.5 µg/mL sufentanil with 0.1% ropivacaine for labor analgesia. Hemodynamic parameters were monitored. Pain was assessed using a visual analog scale. The onset of epidural analgesia, duration of stages of labor, Ramsay Sedation Scale, blood loss, neonatal Apgar scores, umbilical artery blood pH and adverse effects, such as respiratory depression, nausea, vomiting, pruritus, and bradycardia, were recorded.Results: Compared with the control group, visual analog scale values after cervical dilation >3 cm were lower in group D (P<0.05) and first-stage labor duration was shorter in group D (378.5±52.6 vs 406.5±58.2, P<0.05). Ramsay Sedation Scale values were higher in group D compared to the control group (2.8±0.6 vs 2.4±0.5, P<0.05). No significant differences in side effects were observed between the groups.Conclusion: Dexmedetomidine is superior to sufentanil in analgesic effect and duration in first-stage labor during epidural analgesia when combined with 0.1% ropivacaine (www.chictr.org.cn, registration ChiCTR-OPC-16008548). Keywords: dexmedetomidine, sufentanil, epidural, analgesia, labor
Anahtar Kelimeler : Dexmedetomidine, Sufentanil, Epidural, analgesia, Labor;
ISSN: 1177-8881
Cilt: 13
Sayfa: 1171 - 1175
20
Folic acid-conjugated mesoporous silica nanoparticles for enhanced therapeutic efficacy of topotecan in retina cancers
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Dergi Makalesi
Open Access
Kaynak :
Directory of Open Access Journals (DOAJ)
Dergi:
International Journal of Nanomedicine
Yazar(lar) :
Qu W , Meng B , Yu Y , Wang S
Yıl :
2018
Yu Y
Yayıncı Web Sitesi
Kaydet Alıntıla
Wei Qu,1 Bo Meng,1 Yangyang Yu,2 Shaowei Wang1 1Department of Ophthalmology, The 2nd Affiliated Hospital of Harbin Medical University, 2Department of Ophthalmology, Heilongjiang Provincial Hospital, Harbin, Heilongjiang, People’s Republic of China Abstract: In this study, topotecan-loaded mesoporous silica nanoparticles were prepared and surface conjugated with folic acid (FTMN) to enhance the therapeutic efficacy of topotecan for the treatment of retinoblastoma (RB) cancers. The particles were nano-sized and exhibited a sustained release of drug in the physiological conditions. The folic acid-conjugated nanoformulations exhibited a remarkable uptake in RB cells compared to that of non-targeted nanoparticles. These results clearly indicate that receptor-mediated endocytosis is the mechanism of cellular internalization. The greater cellular uptake of FTMN resulted in significantly higher cytotoxic effect in Y79 cancer cells compared to that of other formulations. The results were well corroborated with the live/dead assay and nuclear fragmentation assay. FTMN consistently induced apoptosis of cancer cells with an efficiency of ~58%. Our results clearly showed that nanoparticulate encapsulation of TPT exhibited superior anticancer efficacy in Y79 cancer cells compared to that of free drug or non-targeted nanoparticles. As expected, FTMN exhibited a remarkable reduction in the overall tumor volume compared to any other group with less presence of tumor cells in histology staining. Overall, folic acid-conjugated nanoparticulate system could provide an effective platform for RB treatment. Keywords: retinoblastoma, topotecan, mesoporous silica nanoparticles, apoptosis, anticancer effect
Anahtar Kelimeler : Retinoblastoma, topotecan, mesoporous silica nanoparticles, apoptosis, anticancer effect
Cilt: 13
Sayfa: 4379 - 4389
Sınıflandırma :
21
Silica nanoparticles induce autophagy and endothelial dysfunction via the PI3K/Akt/mTOR signaling pathway
Dergi Makalesi

Dergi Makalesi
Open Access
Kaynak :
Directory of Open Access Journals (DOAJ)
Dergi:
International Journal of Nanomedicine
Yazar(lar) :
Duan J , Yu Y , Yu Y , Li Y , Wang J , Geng W , Jiang L , Li Q , Zhou X , Sun Z
Yıl :
2014
Yu Y Yu Y
Yayıncı Web Sitesi
Kaydet Alıntıla
Junchao Duan,1,2 Yongbo Yu,1,2 Yang Yu,1,2 Yang Li,1,2 Ji Wang,1,2 Weijia Geng,1,2 Lizhen Jiang,1,2 Qiuling Li,1,2 Xianqing Zhou,1,2 Zhiwei Sun1,2 1School of Public Health, Capital Medical University, Beijing, 2Beijing Key Laboratory of Environmental Toxicology, Capital Medical University, Beijing, People’s Republic of China Abstract: Although nanoparticles have a great potential for biomedical applications, there is still a lack of a correlative safety evaluation on the cardiovascular system. This study is aimed to clarify the biological behavior and influence of silica nanoparticles (Nano-SiO2) on endothelial cell function. The results showed that the Nano-SiO2 were internalized into endothelial cells in a dose-dependent manner. Monodansylcadaverine staining, autophagic ultrastructural observation, and LC3-I/LC3-II conversion were employed to verify autophagy activation induced by Nano-SiO2, and the whole autophagic process was also observed in endothelial cells. In addition, the level of nitric oxide (NO), the activities of NO synthase (NOS) and endothelial (e)NOS were significantly decreased in a dose-dependent way, while the activity of inducible (i)NOS was markedly increased. The expression of C-reactive protein, as well as the production of proinflammatory cytokines (tumor necrosis factor α, interleukin [IL]-1β, and IL-6) were significantly elevated. Moreover, Nano-SiO2 had an inhibitory effect on the phosphoinositide 3-kinase (PI3K)/protein kinase B (Akt)/mammalian target of rapamycin (mTOR) signaling pathway. Our findings demonstrated that Nano-SiO2 could disturb the NO/NOS system, induce inflammatory response, activate autophagy, and eventually lead to endothelial dysfunction via the PI3K/Akt/mTOR pathway. This indicates that exposure to Nano-SiO2 is a potential risk factor for cardiovascular diseases. Keywords: silica nanoparticles, endothelial dysfunction, autophagy, nitric oxide, inflammation
Cilt: 2014
Sayı: 1
Sayfa: 5131 - 5141
Sınıflandırma :
Biyotıp %100
Biyotıp %90
22
Sevoflurane Regulates Glioma Progression by Circ_0002755/miR-628-5p/MAGT1 Axis
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Dergi Makalesi
Open Access
Kaynak :
Directory of Open Access Journals (DOAJ)
Dergi:
Cancer Management and Research
Yazar(lar) :
Li H , Xia T , Guan Y , Yu Y
Yıl :
2020
Yu Y
Yayıncı Web Sitesi
Kaydet Alıntıla
Haoyi Li,1,* Tian Xia,1,* Yilin Guan,2 Yao Yu1 1Department of Anaesthesiology, The Second Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116027, People’s Republic of China; 2Department of Anaesthesiology, Dalian Municipal Women and Children’s Medical Center, Dalian, Liaoning 116037, People’s Republic of China*These authors contributed equally to this workCorrespondence: Yao YuDepartment of Anaesthesiology, The Second Affiliated Hospital of Dalian Medical University, NO. 467 Zhongshan Road, Dalian, Liaoning 116027, People’s Republic of ChinaTel +86 411-84671291Email summer930318@163.comBackground: Glioma is a common malignant tumor worldwide. Sevoflurane (Sev) has been reported to inhibit the metastasis of glioma cells, but the underlying molecular mechanism needs further exploration.Methods: Cell Counting Kit-8 (CCK8) assay was used to check cell viability. Flow cytometry assay was hired to check cell apoptosis. The protein levels of B-cell lymphoma-2 (Bcl-2), BCL2-Associated X (Bax), hexokinase 2 (HK2) and magnesium transporter 1 (MAGT1) in samples were measured by Western blot. The abilities of cell migration and invasion were estimated by transwell assay. Glucose colorimetric assay kit and lactate colorimetric assay kit were used to check glucose consumption and lactate production, respectively. Quantitative real-time polymerase chain reaction (qRT-PCR) was performed to detect the levels of circular RNA (circRNA) circ_0002755 (also known as the circRNA1656) and microRNA (miR)-628-5p in samples. The interaction between miR-628-5p and circ_0002755 or MAGT1 was predicated by starBase, which was verified by the dual-luciferase reporter assay. Xenograft tumor model was established to explore the biological role of circ_0002755 in vivo.Results: Sev inhibited cell viability, migration, invasion and promoted cell apoptosis, and also reduced glucose consumption and lactate production. Circ_0002755 was significantly upregulated in glioma tissues and cells, while its level was notably declined under Sev treatment. Besides, overexpression of circ_0002755 overturned Sev-mediated inhibitory effect on glioma progression. Further research indicated that circ_0002755 targeted miR-628-5p, and miR-628-5p targeted MAGT1, and Sev modulated glioma progression via circ_0002755/miR-628-5p/MAGT1 axis. Moreover, Sev hindered tumor growth in vivo.Conclusion: Sev mediated glioma progression via circ_0002755/miR-628-5p/MAGT1 axis.Keywords: Sev, glioma, circ_0002755, miR-628-5p, MAGT1
Anahtar Kelimeler : glioma, circ_0002755, mir-628-5p, magt1
ISSN: 1179-1322
Cilt: 12
Sayfa: 5085 - 5098
Sınıflandırma :
23
Fold amplification rates and dominant wavelength selection in multilayer stacks
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Dergi Makalesi
Kaynak :
Taylor and Francis
Dergi:
Philosophical Magazine
Yayıncı:
Informa UK Limited
Yazar(lar) :
D. W. Schmid , Yu. Y. Podladchikov
Konu :
Condensed Matter Physics
Yıl :
2006
Yu. Y. Podladchikov
Yayıncı Web Sitesi
Tam Metin
Kaydet Alıntıla
A combination of thin- and thick-plate theories, and finite element models is used to systematically analyze folding in multilayer stacks. We show that if the interlayer spacing is large, individual layers fold as single layers, if the spacing is small the entire stack folds as one effective single layer. In between, a third folding mode exists that is characterised by a dominant wavelength that scales with n 1/3, irrespective of total number of layers, n. The maximum growth rates in the true multilayer-folding mode are higher than the corresponding single layer growth rates, increase with n and are bounded by a saturation value that is directly proportional to the viscosity contrast. This growth rate saturation as well as the applicability of the true multilayer-folding mode with respect to interlayer spacing can be explained by the normal and inverse contact strain theory. The true multilayer-folding mode is expected to be the most frequent mode in nature, because it exhibits the highest growth rates and has a relatively large applicability range with respect to interlayer spacing. The increased growth rates in multilayer folding are especially important for systems where the corresponding single layer values are not sufficient to drive the folding instability, such as folding in low-viscosity contrast layers and detachment folding.
DOI : 10.1080/14786430500380175
ISSN: 1478-6435
Sayı: 21-22
Cilt: 86
Sayfa: 3409-3423
24
Mechanism of Sanguinarine in Inhibiting Macrophages to Promote Metastasis and Proliferation of Lung Cancer via Modulating the Exosomes in A549 Cells
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Dergi Makalesi
Open Access
Kaynak :
Directory of Open Access Journals (DOAJ)
Dergi:
OncoTargets and Therapy
Yazar(lar) :
Yu Y , Luo Y , Fang Z , Teng W , Yu Y , Tian J , Guo P , Xu R , Wu J , Li Y
Yıl :
2020
Yu Y Yu Y
Yayıncı Web Sitesi
Kaydet Alıntıla
Yuanyuan Yu,1,* Yingbin Luo,1,* Zhihong Fang,1 Wenjing Teng,1 Yongchun Yu,2 Jianhui Tian,3 Peng Guo,1 Rongzhong Xu,1 Jianchun Wu,1 Yan Li1 1Department of Oncology, Shanghai Municipal Hospital of Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine, Shanghai 200071, People’s Republic of China; 2Institute for Thoracic Oncology, Shanghai Chest Hospital, Shanghai Jiao Tong University, Shanghai 200030, People’s Republic of China; 3Institute of Traditional Chinese Medicine in Oncology, Department of Oncology, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, People’s Republic of China*These authors contributed equally to this workCorrespondence: Yan Li; Jianchun WuDepartment of Oncology, Shanghai Municipal Hospital of Traditional Chinese Medicine, Shanghai University of Traditional Chinese Medicine, No. 274, Zhijiang Road, Jing’an District, Shanghai 200071, People’s Republic of ChinaTel/Fax +86-21-63925588Email yan.xiaotian@shutcm.edu.cn; eq219@126.comObjective: Sanguinarine (SNG) is a benzophenanthridine alkaloid obtained from the roots of Sanguinaria canadensis and has an anticancer effect. The aim of this study was to explore the mechanism of SNG in inhibiting macrophages via regulating the exosomes derived from lung carcinoma cells to reduce metastasis and proliferation of lung carcinoma.Methods: Human lung cancer cells (A549 cells) were treated with 4μM of SNG. Exosomes of A549 cells were extracted from A549 cells supernatant, and THP-1 cells were cultured with exosomes. Then, the supernatant of THP-1 cells was collected and cultured with A549 cells. Cell proliferation was measured via plate clone formation and CCK-8 assays. Migration was assessed by using Transwell assay and scratch test. Cellular invasion was detected by Transwell assay. Apoptosis was determined using flow cytometry. Moreover, the protein expressions of GAPDH, P65 and P-P65 in THP-1 cells were measured by Western blot. Levels of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and chemotactic cytokines ligand 2 (CCL-2) extracted from THP-1 cells were determined by reverse transcription-polymerase chain reaction (RT-PCR).Results: Compared to the control group, exosomes could activate THP-1 cells, and the invasion, migration, and proliferation of A549 cells were consequently enhanced. Exosomes could increase the protein expression of p-p65 and the RNA expression levels of TNF-α, IL-6, and CCL-2 in THP-1 cells. Compared with the exosome group, SNG-treated exosomes inhibited THP-1 cells so that the invasion, proliferation, and migration of A549 cells were attenuated and apoptosis was promoted. In THP-1 cells, SNG-treated exosomes inhibited P-P65 expression and the RNA expression levels of TNF-α, IL-6, and CCL-2.Conclusion: Exosomes treated by SNG inhibited THP-1 cells so that the invasion, proliferation, and migration of A549 cells were inhibited, and the apoptosis was promoted. The mechanism is possibly associated with the inhibition of NF-κB pathway in THP-1 cells.Keywords: SNG, exosomes, THP-1 cells, NF-κB signaling pathway
Anahtar Kelimeler : exosomes, thp-1 cells, nf-κb signaling pathway
ISSN: 1178-6930
Cilt: 13
Sayfa: 8989 - 9003
Sınıflandırma :
25
The influence of wettability and critical pore-throat size ratio on snap—off
Dergi Makalesi

Dergi Makalesi
Kaynak :
Science Direct
Dergi:
Journal of Colloid and Interface Science
Yayıncı:
Elsevier
Yazar(lar) :
Li Yu (Y. Li) , Norman Claude Wardlaw
Yıl :
1986
Li Yu (Y. Li)
Yayıncı Web Sitesi
Tam Metin
Kaydet Alıntıla
Experiments under quasistatic conditions show that the aspect ratio (pore-to-throat effective diameter ratio) necessary for snap-off in throats is ∼ 1.5 when advancing contact angles (θA) are equal to zero and increases only slightly, to 1.75, when θA is equal to 55°. Above ∼70°, snap-off in throats does not occur in systems with pressure equilibrium between pores and throats. Three critical contact angles are recognized for two types of interfaces. Advancing (θA) and receding (θR) angles for convex interfaces and θS, the angle measured for selloidal interfaces at the moment of instability when snap-off occurs. Capillary pressure, disjoining pressure, thin-film thickness, and contact angles are interrelated phenomena and, for a given solid and fluid pair, θA, θR and θS may be defined at different pressures and are not equal but are points on a common curve, the equation for which is defined. As θA increases from 0° to 70° we estimate that θS increases from 30° to 77°. The difference between θS and θA decreases with increase in θA. The Young-Laplace equation assumes constancy of contact angle and must be applied with caution since contact angle is a function of capillary pressure as well as of interface type.
DOI : 10.1016/0021-9797(86)90324-3
ISSN: 0021-9797
Sayı: 2
Cilt: 109
Sayfa: 461-472
Sınıflandırma :
26
Mechanisms of nonwetting phase trapping during imbibition at slow rates
Dergi Makalesi

Dergi Makalesi
Kaynak :
Science Direct
Dergi:
Journal of Colloid and Interface Science
Yayıncı:
Elsevier
Yazar(lar) :
Li Yu (Y. Li) , Norman Claude Wardlaw
Yıl :
1986
Li Yu (Y. Li)
Yayıncı Web Sitesi
Tam Metin
Kaydet Alıntıla
Experiments performed in transparent-glass micromodels were used to observe the mechanisms of both wetting phase (wp) and nonwetting phase (nwp) disconnection and entrapment during drainage and imbibition at slow rates. Convex and selloidal menisci, slowly advanced by an externally imposed flow during imbibition, periodically exceed limits of stability and undergo short, rapid advances during which nwp may become disconnected by one of two processes referred to as snap-off and break-off. Snap-off involves selloidal menisci and can occur within throats, within pores or at the junction region of pores and throats. Break-off refers to rupture during piston-type advance of a convex interface which contacts an opposing pore wall. The critical capillary pressures and other conditions for snap-off in throats, piston-type motions in pores, snap-offin pores and break-off have been determined experimentally and provide the basis for simulating microscopic displacement processes in media with any specified structure and wettability. These interface movements during imbibition are influenced by the sizes, shapes, and arrangement of both pores and throats with respect to the direction of advancing displacing phase. Earlier interface motions determine what is possible subsequently and the sequence and position of snap-off and break-off events determine the amount and location of trapped nonwetting phase. Wettability and topology are the main factors which determine whether snap-off occurs in throats, in pores, or at pore-throat junctions.
DOI : 10.1016/0021-9797(86)90325-5
ISSN: 0021-9797
Sayı: 2
Cilt: 109
Sayfa: 473-486
Sınıflandırma :
27
Reducing Contact temperatures during grinding of ceramics
Dergi Makalesi

Dergi Makalesi
Kaynak :
Springer Link
Dergi:
Glass and Ceramics
Yazar(lar) :
K. P. Belous , Yu. Y. �iduk
Konu :
Materials Chemistry, Mechanics of Materials, Ceramics and Composites
Yıl :
1985
Yu. Y. �iduk
Yayıncı Web Sitesi
Tam Metin
Kaydet Alıntıla
DOI : 10.1007/bf00698692
Anahtar Kelimeler : Polymer, Contact Temperature
ISSN: 0361-7610 1573-8515
Sayı: 3
Cilt: 42
Sayfa: 149-153
28
Advances in dosimetry and biological predictors of radiation-induced esophagitis
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Dergi Makalesi
Open Access
Kaynak :
Directory of Open Access Journals (DOAJ)
Dergi:
OncoTargets and Therapy
Yazar(lar) :
Yu Y , Guan H , Dong Y , Xing L , Li X
Yıl :
2016
Yu Y
Yayıncı Web Sitesi
Kaydet Alıntıla
Yang Yu,1 Hui Guan,1 Yuanli Dong,1 Ligang Xing,2 Xiaolin Li2 1School of Medicine and Life Sciences, Shandong Academy of Medical Sciences, University of Jinan, Jinan, 2Department of Radiation Oncology, Shandong Cancer Hospital, Jinan, Shandong Province, People’s Republic of China Objective: To summarize the research progress about the dosimetry and biological predictors of radiation-induced esophagitis.Methods: We performed a systematic literature review addressing radiation esophagitis in the treatment of lung cancer published between January 2009 and May 2015 in the PubMed full-text database index systems.Results: Twenty-eight eligible documents were included in the final analysis. Many clinical factors were related to the risk of radiation esophagitis, such as elder patients, concurrent chemoradiotherapy, and the intense radiotherapy regimen (hyperfractionated radiotherapy or stereotactic body radiotherapy). The parameters including Dmax, Dmean, V20, V30, V50, and V55 may be valuable in predicting the occurrence of radiation esophagitis in patients receiving concurrent chemoradiotherapy. Genetic variants in inflammation-related genes are also associated with radiation-induced toxicity.Conclusion: Dosimetry and biological factors of radiation-induced esophagitis provide clinical information to decrease its occurrence and grade during radiotherapy. More prospective studies are warranted to confirm their prediction efficacy. Keywords: lung cancer, esophagitis, radiation injuries, predictors
Anahtar Kelimeler : lung cancer, esophagitis, radiation injuries, predictors
ISSN: 1178-6930
Cilt: 2016
Sayı: 1
Sayfa: 597 - 603
Sınıflandırma :
29
Molecular Characterization of Carbapenem-Resistant Serratia marcescens Clinical Isolates in a Tertiary Hospital in Hangzhou, China
Dergi Makalesi

Dergi Makalesi
Open Access
Kaynak :
Directory of Open Access Journals (DOAJ)
Dergi:
Infection and Drug Resistance
Yazar(lar) :
Xu Q , Fu Y , Zhao F , Jiang Y , Yu Y
Yıl :
2020
Yu Y
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Kaydet Alıntıla
Qian Xu,1 Ying Fu,2 Feng Zhao,2 Yan Jiang,1 Yunsong Yu1 1Department of Infectious Diseases, Sir Run Run Shaw Hospital, Zhejiang University School of Medicine, Key Laboratory of Microbial Technology and Bioinformatics of Zhejiang Province, Hangzhou City, Zhejiang Province, 310016, People’s Republic of China; 2Clinical Laboratory, Sir Run Run Shaw Hospital, School of Medicine, Zhejiang University, Hangzhou City, Zhejiang Province 310016, People’s Republic of ChinaCorrespondence: Yan Jiang; Yunsong Yu Tel/Fax +86-571-8600-6142Email jiangy@zju.edu.cn; yvys119@zju.edu.cnIntroduction: Although carbapenem-resistant Enterobacteriaceae (CRE) have been thoroughly investigated as the pathogens most commonly associated with clinical infections, data on Serratia marcescens are inadequate and superficial.Methods: In this study, we characterized 36 carbapenem-resistant Serratia marcescens (CRSM) isolates in our hospital from April 2018 to March 2019 by analysing whole-genome sequencing (WGS) data. The molecular typing of the isolates was performed using both pulsed-field gel electrophoresis (PFGE) and core genome multilocus sequence typing (cgMLST).Results: Thirty-three of the 36 isolates showed carbapenem resistance conferred by a blaKPC-2-harbouring plasmid, while the remaining three isolates were characterized by overexpression of beta-lactamase combined with porin loss. The blaKPC-2 genes in all the isolates were located on a plasmid of ∼ 103 kb, except one, which was on a plasmid of ∼ 94 kb. The gene structure surrounding blaKPC-2 in the plasmids was confirmed by integration of a partial Tn4401 structure and an intact IS26 as previously reported. Most of the plasmids also contained a mobile genetic element (MGE) comprising qnr and ISKpn19, which provided evidence of horizontal transfer of antibiotic resistance genes.Conclusion: The thirty-six CRSM isolates were mainly clonally disseminated with a blaKPC-2-harbouring plasmid in our hospital. The gene structure surrounding blaKPC-2 as an MGE, as well as the qnr segment, might be acquired by horizontal gene transfer, and it could aggravate the infection and increase the difficulty of clinical treatment.Keywords: Serratia marcescens, carbapenem resistance, blaKPC-2-harbouring plasmid
Anahtar Kelimeler : serratia marcescens, carbapenem resistance, blakpc-harbouring plasmid
ISSN: 1178-6973
Cilt: 13
Sayfa: 999 - 1008
Sınıflandırma :
Viroloji %99
30
Diagnosis and Surgical Treatment and Pathological Findings of Solid Pseudopapillary Tumor of the Pancreas: A Single-Institution Experience
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Dergi Makalesi
Open Access
Kaynak :
Directory of Open Access Journals (DOAJ)
Dergi:
Cancer Management and Research
Yazar(lar) :
Guo T , Wang L , Xie P , Zhang Z , Yu Y
Yıl :
2020
Yu Y
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Tong Guo, Lu Wang, Peng Xie, Zhiwei Zhang, Yahong Yu Department of Biliary-Pancreatic Surgery, Affiliated Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, Hubei, People’s Republic of ChinaCorrespondence: Yahong YuDepartment of Biliary-Pancreatic Surgery, Affiliated Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1095 Jiefang Avenue, Wuhan 430030, Hubei, People’s Republic of ChinaTel +86 13907190038Email yuyahong615@sina.comPurpose: To investigate the diagnosis, surgical treatment, and pathology of solid pseudopapillary tumors of the pancreas in our institution.Patients and Methods: We retrospectively analyzed the demographic details, clinical features, imaging findings, and pathological findings of 87 patients with a confirmed diagnosis of solid pseudopapillary tumors of the pancreas (SPTP) and underwent surgery in Tongji Hospital of Tongji Medical College, over a period of 8 years from 2011 to 2018.Results: Our study involved a total of 87 patients (16 males and 71 females) with a mean age of 31.3± 13.1 years (range: 10– 61 years). The main compliant was abdominal pain or discomfort (n=49) and the median tumor size was 58.6± 31.7 mm (range: 16– 156mm). Tumors were located in the head (27 patients, 31%), the neck (13 patients, 15%), and the body and tail (47 patients, 54%). There were no significant differences between the patients in terms of sex, age, or tumor location. Partial pancreatectomy was performed in 79 patients, enucleation in six patients, and total pancreatectomy in two patients. R0 resection was achieved in 86 patients. The postoperative morbidity was 36.8%, and the main complication was pancreatic fistula. Pathological examination and immunohistochemical markers were used to provide a final diagnosis. The main follow-up period was 46 months (range: 13– 97 months). At the end of the follow-up period, 86 patients were alive and had not experienced recurrence; one patient was lost to follow-up.Conclusion: The accurate diagnosis of SPTP is vital. Our data showed that surgical resection is safe and associated with low morbidity and mortality rates. Pathological findings can play an important role in diagnosis and long-term survival.Keywords: solid pseudopapillary tumor, pancreas, diagnosis, surgical strategy
Anahtar Kelimeler : solid pseudopapillary tumor, pancreas, diagnosis, surgical strategy
ISSN: 1179-1322
Cilt: 12
Sayfa: 581 - 588
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