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1
Isolation and characterization of different promising fungi for biological waste management of polyurethanes
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Kaynak :
Wiley Online Library
Dergi:
Microbial Biotechnology
Yazar(lar) :
Audrey Magnin , Lucie Hoornaert , Eric Pollet , Stéphanie Laurichesse , Vincent Phalip , Luc Avérous
Yıl :
2018
Microbial Biotechnology
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As a highly resistant polymer family, polyurethanes (PU) are responsible for increasing environmental issues. Then, PU biodegradation is a challenging way to develop sustainable waste management processes based on biological recycling. Since the metabolic diversity of fungi is a major asset for polymer degradation, nearly thirty strains were isolated from sampling on six different PU wastes‐containing environments. A screening of the fungi on four thermoplastic PU (TPU) with different macromolecular architectures led to the selection of three strains able to use two polyester PU as sole carbon source: Alternaria sp., Penicillium section Lanata‐Divaricata and Aspergillus section flavi. Weight loss, FT‐IR, Scanning Electron Microscopy and Size Exclusion Chromatography analyses revealed that these three fungi degrade slightly and similarly a fatty acid dimer‐based TPU while variability of degradation was noticed on a polycaprolactone‐based TPU. On this last TPU, robust analysis of the degraded polymers showed that the Penicillium strain was the best degrading microorganism. Membrane enzymes seemed to be involved in this degradation. It is the first time that a strain of Penicillium of the section Lanata‐Divaricata displaying PU biodegradation ability is isolated. These newly discovered fungi are promising for the development of polyester PU waste management process.
DOI : 10.1111/1751-7915.13346
ISSN: 1751-7915
Sayı: 3
Cilt: 12
Sayfa: 544-555
2
Cyclopropane fatty acids are involved in organic solvent tolerance but not in acid stress resistance in Pseudomonas putida DOT-T1E
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Dergi Makalesi
Kaynak :
Wiley Online Library
Dergi:
Microbial Biotechnology
Yazar(lar) :
Cecilia-Vanesa Pini , Patricia Bernal , Patricia Godoy , Juan-Luis Ramos , Ana Segura
Yıl :
2009
Microbial Biotechnology
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DOI : 10.1111/j.1751-7915.2009.00084.x
ISSN: 1751-7915
Sayı: 2
Cilt: 2
Sayfa: 253-261
3
Protein profile inAspergillus nidulansrecombinant strains overproducing heterologous enzymes
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Dergi Makalesi
Kaynak :
Wiley Online Library
Dergi:
Microbial Biotechnology
Yazar(lar) :
Mariane Paludetti Zubieta , Fabiano Jares Contesini , Marcelo Ventura Rubio , Any Elisa de Souza Schmidt Gonçalves , Jaqueline Aline Gerhardt , Rolf Alexander Prade , André Ricardo de Lima Damasio
Yıl :
2018
Microbial Biotechnology
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Filamentous fungi are robust cell factories and have been used for the production of large quantities of industrially relevant enzymes. However, the production levels of heterologous proteins still need to be improved. Therefore, this article aimed to investigate the global proteome profiling of Aspergillus nidulans recombinant strains in order to understand the bottlenecks of heterologous enzymes production. About 250, 441 and 424 intracellular proteins were identified in the control strain Anid_pEXPYR and in the recombinant strains Anid_AbfA and Anid_Cbhl respectively. In this context, the most enriched processes in recombinant strains were energy pathway, amino acid metabolism, ribosome biogenesis, translation, endoplasmic reticulum and oxidative stress, and repression under secretion stress (RESS). The global protein profile of the recombinant strains Anid_AbfA and Anid_Cbhl was similar, although the latter strain secreted more recombinant enzyme than the former. These findings provide insights into the bottlenecks involved in the secretion of recombinant proteins in A. nidulans, as well as in regard to the rational manipulation of target genes for engineering fungal strains as microbial cell factories.
DOI : 10.1111/1751-7915.13027
ISSN: 1751-7915
Sayı: 2
Cilt: 11
Sayfa: 346-358
4
0
Dergi Sayısı

Dergi Sayısı
Kaynak 1:
Wiley Online Library
Kaynak 2:
Wiley Online Library
Dergi:
Microbial Biotechnology
Konu :
Biotechnology, Applied Microbiology and Biotechnology, Biochemistry, Bioengineering
Yıl :
2009
Microbial Biotechnology
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DOI : 10.1111/mbt.2009.2.issue-6
ISSN: 1751-7907
Sayı: 6
Cilt: 2

5
Directed evolution of the transcription factor XylS for development of improved expression systems
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Dergi Makalesi
Kaynak :
Wiley Online Library
Dergi:
Microbial Biotechnology
Yazar(lar) :
Trond Erik Vee Aune , Ingrid Bakke , Finn Drabløs , Rahmi Lale , Trygve Brautaset , Svein Valla
Yıl :
2009
Microbial Biotechnology
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The inducible Pm promoter together with its cognate positive transcription regulator XylS has been shown to be useful for recombinant protein production under high cell density conditions. Here we report directed evolution of XylS resulting in mutant proteins with increased ability to stimulate transcription in Escherichia coli from Pm. A first round of mutagenesis using error‐prone PCR on xylS was used to construct a library consisting of about 430 000 clones, and this library could be efficiently screened with respect to stimulation of expression from Pm due to a positive correlation between the level of expression of the reporter gene, bla (encoding β‐lactamase), and the ampicillin tolerance of the corresponding host cells. Fourteen different amino acid substitutions in XylS were found to separately lead to up to nearly a threefold stimulation of expression under induced conditions, relative to wild type. These mutations were all located in the part corresponding to the N‐terminal half of the protein. Varying combinations of the mutations resulted in further stimulation, and the best results (about 10‐fold stimulation under induced conditions) were obtained by using a random shuffling procedure followed by a new round of screening. The uninduced levels of expression for the same mutants also increased, but only about four times. Through in silico 3D modelling of the N‐terminal domain of XylS, it was observed that the evolved mutant proteins contained substitutions that were positioned in different parts of the predicted structure, including a β‐barrel putatively responsible for effector binding and a coiled coil probably important for dimerization. The total production of the host‐toxic antibody fragment scFv‐phOx expressed from Pm with the evolved XylS mutant protein StEP‐13 was about ninefold higher than with wild‐type XylS, demonstrating that directed evolution of transcription factors can be an important new tool to achieve high‐level recombinant protein production.
DOI : 10.1111/j.1751-7915.2009.00126.x
ISSN: 1751-7915
Sayı: 1
Cilt: 3
Sayfa: 38-47
6
Preservation of H2production activity in nanoporous latex coatings ofRhodopseudomonas palustris CGA009 during dry storage at ambient temperatures
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Dergi Makalesi
Kaynak :
Wiley Online Library
Dergi:
Microbial Biotechnology
Yazar(lar) :
M. Piskorska , T. Soule , J. L. Gosse , C. Milliken , M. C. Flickinger , G. W. Smith , C. M. Yeager
Yıl :
2013
Microbial Biotechnology
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DOI : 10.1111/1751-7915.12032
ISSN: 1751-7915
Sayı: 5
Cilt: 6
Sayfa: 515-525
7
Microbiome Yarns: human biome reproduction, evolution and visual acuity,,,
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Dergi Makalesi
Kaynak :
Wiley Online Library
Dergi:
Microbial Biotechnology
Yazar(lar) :
Kenneth Timmis , Franziska Jebok , Gabriella Molinari , Manfred Rohde , Leo Lahti
Yıl :
2017
Microbial Biotechnology
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DOI : 10.1111/1751-7915.13037
ISSN: 1751-7915
Sayı: 1
Cilt: 11
Sayfa: 149-159
8
Improved bacterial nanocellulose production from glucose without the loss of quality by evaluating thirteen agitator configurations at low speed
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Dergi Makalesi
Kaynak :
Wiley Online Library
Dergi:
Microbial Biotechnology
Yazar(lar) :
Genqiang Chen , Lin Chen , Wei Wang , Shiyan Chen , Huaping Wang , Yen Wei , Feng F. Hong
Yıl :
2019
Microbial Biotechnology
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Thirteen agitator configurations were investigated at low speed in stirred‐tank reactors (STRs) to determine if improved crude bacterial nanocellulose (BNC) productivity can be achieved from glucose‐based media while maintaining high BNC quality using Komagataeibacter xylinus ATCC 23770 as a model organism. A comparison of five single impellers showed the pitched blade (large) was the optimal impeller at 300 rpm. The BNC production was further increased by maintaining the pH at 5.0. Among the single helical ribbon and frame impellers and the combined impellers, the twin pitched blade provided the best results. The combined impellers at 150 rpm performed better than the single impellers, and after optimizing the agitation conditions, the twin pitched blade (large) and helical ribbon impellers performed the best at 100 rpm. The performances of different agitators at low speed during BNC production were related to how efficiently the agitators improved the oxygen mass transfer coefficient. The twin pitched blade (large) was verified as providing the optimum performance by an observed crude BNC production of 1.97 g (L×d)−1 and a BNC crude yield of consumed glucose of 0.41 g g−1, which were 2.25 and 2.37 times higher than the initial values observed using the single impeller respectively. Further characterization indicated that the BNC obtained at 100 rpm from the STR equipped with the optimal agitator maintained high degree of polymerization and crystallinity.
DOI : 10.1111/1751-7915.13477
ISSN: 1751-7915
Sayı: 6
Cilt: 12
Sayfa: 1387-1402
9
Glucose metabolic flux distribution ofLactobacillus amylophilusduring lactic acid production using kitchen waste saccharified solution
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Dergi Makalesi
Kaynak :
Wiley Online Library
Dergi:
Microbial Biotechnology
Yazar(lar) :
Jianguo Liu , Qunhui Wang , Hui Zou , Yingying Liu , Juan Wang , Kemin Gan , Juan Xiang
Yıl :
2013
Microbial Biotechnology
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The 13C isotope tracer method was used to investigate the glucose metabolic flux distribution and regulation in Lactobacillus amylophilus to improve lactic acid production using kitchen waste saccharified solution (KWSS). The results demonstrate that L. amylophilus is a homofermentative bacterium. In synthetic medium, 60.6% of the glucose entered the Embden–Meyerhof–Parnas (EMP) to produce lactic acid, whereas 36.4% of the glucose entered the pentose phosphate metabolic pathway (HMP). After solid–liquid separation of the KWSS, the addition of Fe3+ during fermentation enhanced the NADPH production efficiency and increased the NADH content. The flux to the EMP was also effectively increased. Compared with the control (60.6% flux to EMP without Fe3+ addition), the flux to the EMP with the addition of Fe3+ (74.3%) increased by 23.8%. In the subsequent pyruvate metabolism, Fe3+ also increased lactate dehydrogenase activity, and inhibited alcohol dehydrogenase, pyruvate dehydrogenase and pyruvate carboxylase, thereby increasing the lactic acid production to 9.03 g l−1, an increase of 8% compared with the control. All other organic acid by‐products were lower than in the control. However, the addition of Zn2+ showed an opposite effect, decreasing the lactic acid production. In conclusion it is feasible and effective means using GC‐MS, isotope experiment and MATLAB software to integrate research the metabolic flux distribution of lactic acid bacteria, and the results provide the theoretical foundation for similar metabolic flux distribution.
DOI : 10.1111/1751-7915.12046
ISSN: 1751-7915
Sayfa: n/a-n/a
10
The antagonistic strainBacillus subtilis UMAF6639 also confers protection to melon plants against cucurbit powdery mildew by activation of jasmonate- and salicylic acid-dependent defence responses
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Dergi Makalesi
Kaynak :
Wiley Online Library
Dergi:
Microbial Biotechnology
Yazar(lar) :
Laura García-Gutiérrez , Houda Zeriouh , Diego Romero , Jaime Cubero , Antonio de Vicente , Alejandro Pérez-García
Yıl :
2013
Microbial Biotechnology
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Biological control of plant diseases has gained acceptance in recent years. Bacillus subtilis UMAF6639 is an antagonistic strain specifically selected for the efficient control of the cucurbit powdery mildew fungus Podosphaera fusca, which is a major threat to cucurbits worldwide. The antagonistic activity relies on the production of the antifungal compounds iturin and fengycin. In a previous study, we found that UMAF6639 was able to induce systemic resistance (ISR) in melon and provide additional protection against powdery mildew. In the present work, we further investigated in detail this second mechanism of biocontrol by UMAF6639. First, we examined the signalling pathways elicited by UMAF6639 in melon plants, as well as the defence mechanisms activated in response to P. fusca. Second, we analysed the role of the lipopeptides produced by UMAF6639 as potential determinants for ISR activation. Our results demonstrated that UMAF6639 confers protection against cucurbit powdery mildew by activation of jasmonate‐ and salicylic acid‐dependent defence responses, which include the production of reactive oxygen species and cell wall reinforcement. We also showed that surfactin lipopeptide is a major determinant for stimulation of the immune response. These results reinforce the biotechnological potential of UMAF6639 as a biological control agent.
DOI : 10.1111/1751-7915.12028
ISSN: 1751-7915
Sayı: 3
Cilt: 6
Sayfa: 264-274
11
Anti‐obesity effects of Chenpi: an artificial gastrointestinal system study
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Dergi Makalesi
Kaynak :
Wiley Online Library
Dergi:
Microbial Biotechnology
Yazar(lar) :
Maria Falduto , Francesco Smedile , Man Zhang , Ting Zheng , Jieyu Zhu , Qingrong Huang , Richard Weeks , Alexey M. Ermakov , Michael L. Chikindas
Yıl :
2022
Microbial Biotechnology
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The gut microbiota plays a significant role in human health; however, the complex relationship between gut microbial communities and host health is still to be thoroughly studied and understood. Microbes in the distal gut contribute to host health through the biosynthesis of vitamins and essential amino acids and the generation of important metabolic by‐products from dietary components that are left undigested by the small intestine. Aged citrus peel (Chenpi) is used in traditional Chinese medicine to lower cholesterol, promote weight loss and treat various gastrointestinal symptoms. This study investigated how the microbial community changes during treatment with Chenpi using the Simulator of the Human Intestinal Microbial Ecosystem (SHIME). Two preparations of Chenpi extract were tested: Chenpi suspended in oil only and Chenpi in a viscoelastic emulsion. Short‐chain fatty acids (SCFAs) were measured during treatment to monitor changes in the microbial community of the colon presenting a decrease in production for acetic, propionic and butyric acid (ANOVA (P < 0.001) during the 15 days of treatment. 16S rRNA sequencing of microbial samples showed a clear difference between the two treatments at the different sampling times (ANOSIM P < 0.003; ADOSIM P < 0.002 [R2 = 69%]). Beta diversity analysis by PcoA showed differences between the two Chenpi formulations for treatment day 6. These differences were no longer detectable as soon as the Chenpi treatment was stopped, showing a reversible effect of Chenpi on the human microbiome. 16S rRNA sequencing of microbial samples from the descending colon showed an increase in Firmicutes for the treatment with the viscoelastic emulsion. At the genus level, Roseburia, Blautia, Subdoligranulum and Eubacterium increased in numbers during the viscoelastic emulsion treatment. This study sheds light on the anti‐obesity effect of a polymethoxyflavone (PMFs)‐enriched Chenpi extract and creates a foundation for the identification of ‘obesity‐prevention’ biomarkers in the gut microbiota.
DOI : 10.1111/1751-7915.14005
ISSN: 1751-7915
12
Chasing bacterialchassisfor metabolic engineering: a perspective review from classical to non-traditional microorganisms
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Dergi Makalesi
Kaynak :
Wiley Online Library
Dergi:
Microbial Biotechnology
Yazar(lar) :
Patricia Calero , Pablo I. Nikel
Yıl :
2018
Microbial Biotechnology
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The last few years have witnessed an unprecedented increase in the number of novel bacterial species that hold potential to be used for metabolic engineering. Historically, however, only a handful of bacteria have attained the acceptance and widespread use that are needed to fulfil the needs of industrial bioproduction – and only for the synthesis of very few, structurally simple compounds. One of the reasons for this unfortunate circumstance has been the dearth of tools for targeted genome engineering of bacterial chassis, and, nowadays, synthetic biology is significantly helping to bridge such knowledge gap. Against this background, in this review, we discuss the state of the art in the rational design and construction of robust bacterial chassis for metabolic engineering, presenting key examples of bacterial species that have secured a place in industrial bioproduction. The emergence of novel bacterial chassis is also considered at the light of the unique properties of their physiology and metabolism, and the practical applications in which they are expected to outperform other microbial platforms. Emerging opportunities, essential strategies to enable successful development of industrial phenotypes, and major challenges in the field of bacterial chassis development are also discussed, outlining the solutions that contemporary synthetic biology‐guided metabolic engineering offers to tackle these issues.
DOI : 10.1111/1751-7915.13292
ISSN: 1751-7915
Sayı: 1
Cilt: 12
Sayfa: 98-124
13
Improving the autotransporter‐based surface display of enzymes in Pseudomonas putida KT2440
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Dergi Makalesi
Kaynak :
Wiley Online Library
Dergi:
Microbial Biotechnology
Yazar(lar) :
Iasson E. P. Tozakidis , Lena M. Lüken , Alina Üffing , Annika Meyers , Joachim Jose
Yıl :
2020
Microbial Biotechnology
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Pseudomonas putida can be used as a host for the autotransporter‐mediated surface display of enzymes (autodisplay), resulting in whole‐cell biocatalysts with recombinant functionalities on their cell envelope. The efficiency of autotransporter‐mediated secretion depends on the N‐terminal signal peptide as well as on the C‐terminal translocator domain of autotransporter fusion proteins. We set out to optimize autodisplay for P. putida as the host bacterium by comparing different signal peptides and translocator domains for the surface display of an esterase. The translocator domain did not have a considerable effect on the activity of the whole‐cell catalysts. In contrast, by using the signal peptide of the P. putida outer membrane protein OprF, the activity was more than 12‐fold enhanced to 638 mU ml−1 OD−1 compared with the signal peptide of V. cholerae CtxB (52 mU ml−1 OD−1). This positive effect was confirmed with a β‐glucosidase as a second example enzyme. Here, cells expressing the protein with N‐terminal OprF signal peptide showed more than fourfold higher β‐glucosidase activity (181 mU ml−1 OD−1) than with the CtxB signal peptide (42 mU ml−1 OD−1). SDS‐PAGE and flow cytometry analyses indicated that the increased activities correlated with an increased amount of recombinant protein in the outer membrane and a higher number of enzymes detectable on the cell surface.
DOI : 10.1111/1751-7915.13419
ISSN: 1751-7915
Sayı: 1
Cilt: 13
Sayfa: 176-184
14
Establishment of a Cuscuta campestris ‐mediated enrichment system for genomic and transcriptomic analyses of ‘ Candidatus Liberibacter asiaticus’
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Dergi Makalesi
Kaynak :
Wiley Online Library
Dergi:
Microbial Biotechnology
Yazar(lar) :
Tao Li , Ling Zhang , Yunshuang Deng , Xiaoling Deng , Zheng Zheng
Yıl :
2021
Microbial Biotechnology
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‘Candidatus Liberibacter asiaticus’ (CLas) is a phloem‐limited non‐culturable α‐proteobacterium associated with citrus Huanglongbing, a highly destructive disease threatening global citrus industry. Research on CLas is challenging due to the current inability to culture CLas in vitro and the low CLas titre in citrus plant. Here, we develop a CLas enrichment system using the holoparasitic dodder plant (Cuscuta campestris) as an amenable host to acquire and enrich CLas from CLas‐infected citrus shoots maintained hydroponically. Forty‐eight out of fifty‐five (87%) dodder plants successfully parasitized CLas‐infected citrus shoots with detectable CLas by PCR. Among 48 dodders cultures, 30 showed two‐ to 419‐fold CLas titre increase as compared to the corresponding citrus hosts. The CLas population rapidly increased and reached the highest level in dodder tendrils at 15 days after parasitizing citrus shoot. Genome sequencing and assembly derived from CLas‐enriched dodder DNA samples generated a higher resolution than those obtained for CLas from citrus hosts. No genomic variation was detected in CLas after transmission from citrus to dodder during short‐term parasitism. Dual RNA‐Seq experiments showed similar CLas gene expression profiles in dodder and citrus samples, yet dodder samples generated a higher resolution of CLas transcriptome data. The ability of dodder to support CLas multiplication to high levels, as well as its advantage in CLas genomic and transcriptomic analyses, make it an optimal model for further studies on CLas–host interaction.
DOI : 10.1111/1751-7915.13773
ISSN: 1751-7915
Sayı: 2
Cilt: 14
Sayfa: 737-751
15
What is health?
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Kaynak :
Wiley Online Library
Dergi:
Microbial Biotechnology
Yazar(lar) :
Harald Brüssow
Yıl :
2013
Microbial Biotechnology
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Classical medical research is disease focused and still defines health as absence of disease. Languages, however, associate a positive concept of wholeness with health as does the WHO health definition. Newer medical health definitions emphasize the capacity to adapt to changing external and internal circumstances. The results of the 2010 Global Burden of Disease study provides keys for a quantifiable health metrics by developing statistical tools calculating healthy life expectancy. Of central social and economic importance is the question whether healthy ageing can be achieved. This concept hinges on theories on the biological basis of lifespan determination and whether negligible senescence and the compression of morbidity can be achieved in human societies. Since the health impact of the human gut microbiome is currently a topical research area, microbiologists should be aware of the problems in defining health.
DOI : 10.1111/1751-7915.12063
ISSN: 1751-7915
Sayı: 4
Cilt: 6
Sayfa: 341-348
16
Proteomic characterization of extracellular vesicles produced by several wine yeast species
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Dergi Makalesi
Kaynak :
Wiley Online Library
Dergi:
Microbial Biotechnology
Yazar(lar) :
Ana Mencher , Pilar Morales , Eva Valero , Jordi Tronchoni , Kiran Raosaheb Patil , Ramon Gonzalez
Yıl :
2020
Microbial Biotechnology
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In winemaking, the use of alternative yeast starters is becoming increasingly popular. They contribute to the diversity and complexity of wine sensory features and are typically used in combination with Saccharomyces cerevisiae, to ensure complete fermentation. This practice has drawn the interest on interactions between different oenological yeasts, which are also relevant in spontaneous and conventional fermentations, or in the vineyard. Although several interactions have been described and some mechanisms have been suggested, the possible involvement of extracellular vesicles (EVs) has not yet been considered. This work describes the production of EVs by six wine yeast species (S. cerevisiae, Torulaspora delbrueckii, Lachancea thermotolerans, Hanseniaspora uvarum, Candida sake and Metschnikowia pulcherrima) in synthetic grape must. Proteomic analysis of EV‐enriched fractions from S. cerevisiae and T. delbrueckii showed enrichment in glycolytic enzymes and cell‐wall‐related proteins. The most abundant protein found in S. cerevisiae, T. delbrueckii and L. thermotolerans EV‐enriched fractions was the enzyme exo‐1,3‐β‐glucanase. However, this protein was not involved in the here‐observed negative impact of T. delbrueckii extracellular fractions on the growth of other yeast species. These findings suggest that EVs may play a role in fungal interactions during wine fermentation and other aspects of wine yeast biology.
DOI : 10.1111/1751-7915.13614
ISSN: 1751-7915
Sayı: 5
Cilt: 13
Sayfa: 1581-1596
17
Antibiofilm and repair activity of ozonated oil in liposome
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Dergi Makalesi
Kaynak :
Wiley Online Library
Dergi:
Microbial Biotechnology
Yazar(lar) :
Lucrezia Zerillo , Immacolata Polvere , Romualdo Varricchio , Jessica Raffaella Madera , Silvia Du2019Andrea , Serena Voccola , Iacopo Franchini , Romania Stilo , Pasquale Vito , Tiziana Zotti
Yıl :
2021
Microbial Biotechnology
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The use of medical devices, such as contact lenses, represents a substantial risk of infection, as they can act as scaffolds for formation of microbial biofilms. Recently, the increasing emergency of antibiotic resistance has prompted the development of novel and effective antimicrobial drugs for biofilm treatment, such as oxidizing agents. The purpose of this study is to investigate the effects of Ozodrop® and Ozodrop® gel, commercial names of ozonated oil in liposomes plus hypromellose, on eradication and de novo formation of biofilms on different supports, such as plastic plates and contact lens. Our results demonstrate that ozonated liposomal sunflower oil plus hypromellose have an excellent inhibitory effect on bacterial viability and on both de novo formation and eradication of biofilms produced on plates and contact lens by Pseudomonas aeruginosa and Staphylococcus aureus. Moreover, we show that Ozodrop® formulations stimulate expression of antimicrobial peptides and that Ozodrop® gel has a strong repair activity on human epithelial cells, suggesting further applications for the treatment of non‐healing infected wounds.
DOI : 10.1111/1751-7915.13949
ISSN: 1751-7915
18
Metabolic potential of the organic‐solvent tolerant P seudomonas putida   DOT ‐ T1E deduced from its annotated genome
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Dergi Makalesi
Kaynak :
Wiley Online Library
Dergi:
Microbial Biotechnology
Yazar(lar) :
Zulema Udaondo , Lazaro Molina , Craig Daniels , Manuel J. Gómez , María A. Molinau2010Henares , Miguel A. Matilla , Amalia Roca , Matilde Fernández , Estrella Duque , Ana Segura , Juan Luis Ramos
Yıl :
2013
Microbial Biotechnology
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DOI : 10.1111/1751-7915.12061
ISSN: 1751-7915
Sayı: 5
Cilt: 6
Sayfa: 598-611
19
Transgenic expression of glucose dehydrogenase in Azotobacter vinelandii enhances mineral phosphate solubilization and growth of sorghum seedlings
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Dergi Makalesi
Kaynak :
Wiley Online Library
Dergi:
Microbial Biotechnology
Yazar(lar) :
Burla Sashidhar , Appa Rao Podile
Yıl :
2009
Microbial Biotechnology
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The enzyme quinoprotein glucose dehydrogenase (GDH) catalyses the oxidation of glucose to gluconic acid by direct oxidation in the periplasmic space of several Gram‐negative bacteria. Acidification of the external environment with the release of gluconic acid contributes to the solubilization of the inorganic phosphate by biofertilizer strains of the phosphate‐solubilizing bacteria. Glucose dehydrogenase (gcd) gene from Escherichia coli, and Azotobacter‐specific glutamine synthetase (glnA) and phosphate transport system (pts) promoters were isolated using sequence‐specific primers in a PCR‐based approach. Escherichia coli gcd, cloned under the control of glnA and pts promoters, was mobilized into Azotobacter vinelandii AvOP and expressed. Sorghum seeds were bacterized with the transgenic azotobacters and raised in earthen pots in green house. The transgenic azotobacters, expressing E. coli gcd, showed improved biofertilizer potential in terms of mineral phosphate solubilization and plant growth‐promoting activity with a small reduction in nitrogen fixation ability.
DOI : 10.1111/j.1751-7915.2009.00119.x
ISSN: 1751-7915
Sayı: 4
Cilt: 2
Sayfa: 521-529
20
Naphthoquinone glycosides for bioelectroanalytical enumeration of the faecal indicator Escherichia coli
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Dergi Makalesi
Kaynak :
Wiley Online Library
Dergi:
Microbial Biotechnology
Yazar(lar) :
Jamie Hinks , Evelina J. Y. Han , Victor B. Wang , Thomas W. Seviour , Enrico Marsili , Joachim S. C. Loo , Stefan Wuertz
Yıl :
2016
Microbial Biotechnology
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DOI : 10.1111/1751-7915.12373
ISSN: 1751-7915
Sayı: 6
Cilt: 9
Sayfa: 746-757
21
Airborne bacterial emission fluxes from manure‐fertilized agricultural soil
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Dergi Makalesi
Kaynak :
Wiley Online Library
Dergi:
Microbial Biotechnology
Yazar(lar) :
Nadine Thiel , Steffen Münch , Wiebke Behrens , Vera Junker , Matthias Faust , Oliver Biniasch , Tina Kabelitz , Paul Siller , Christian Boedeker , Peter Schumann , Uwe Roesler , Thomas Amon , Kerstin Schepanski , Roger Funk , Ulrich Nübel
Yıl :
2020
Microbial Biotechnology
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This is the first study to quantify the dependence on wind velocity of airborne bacterial emission fluxes from soil. It demonstrates that manure bacteria get aerosolized from fertilized soil more easily than soil bacteria, and it applies bacterial genomic sequencing for the first time to trace environmental faecal contamination back to its source in the chicken barn. We report quantitative, airborne emission fluxes of bacteria during and following the fertilization of agricultural soil with manure from broiler chickens. During the fertilization process, the concentration of airborne bacteria culturable on blood agar medium increased more than 600 000‐fold, and 1 m3 of air carried 2.9 × 105 viable enterococci, i.e. indicators of faecal contamination which had been undetectable in background air samples. Trajectory modelling suggested that atmospheric residence times and dispersion pathways were dependent on the time of day at which fertilization was performed. Measurements in a wind tunnel indicated that airborne bacterial emission fluxes from freshly fertilized soil under local climatic conditions on average were 100‐fold higher than a previous estimate of average emissions from land. Faecal bacteria collected from soil and dust up to seven weeks after fertilization could be traced to their origins in the poultry barn by genomic sequencing. Comparative analyses of 16S rRNA gene sequences from manure, soil and dust showed that manure bacteria got aerosolized preferably, likely due to their attachment to low‐density manure particles. Our data show that fertilization with manure may cause substantial increases of bacterial emissions from agricultural land. After mechanical incorporation of manure into soil, however, the associated risk of airborne infection is low.
DOI : 10.1111/1751-7915.13632
ISSN: 1751-7915
Sayı: 5
Cilt: 13
Sayfa: 1631-1647
22
Corrigendum
Dergi Makalesi

Dergi Makalesi
Kaynak :
Wiley Online Library
Dergi:
Microbial Biotechnology
Yıl :
2017
Microbial Biotechnology
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DOI : 10.1111/1751-7915.12743
ISSN: 1751-7915
Sayı: 4
Cilt: 10
Sayfa: 977-977
23
Issue Information
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Dergi Makalesi
Kaynak :
Wiley Online Library
Dergi:
Microbial Biotechnology
Yıl :
2016
Microbial Biotechnology
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DOI : 10.1111/1751-7915.12320
ISSN: 1751-7915
Sayı: 4
Cilt: 9
Sayfa: 433-434
24
Fifty Percent Human- how art brings us in touch with our microbial cohabitants
Dergi Makalesi

Dergi Makalesi
Kaynak :
Wiley Online Library
Dergi:
Microbial Biotechnology
Yazar(lar) :
Sonja Bäumel , Hanne L. P. Tytgat , Birgit Nemec , Ruth Schmidt , Loo Wee Chia , Hauke Smidt
Yıl :
2018
Microbial Biotechnology
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The Human Microbiome, as well as the exploration of the microorganisms inhabiting the human body, are not only integral to the field of microbiology but represent an intrinsic part of all human beings. Consequently, along with scientists, artists have been inspired by the microbiome: transforming it in to tangible artefacts in order to critically question, reflect on and break down the barrier between humans and their microcohabitants. By artistic means, artists help us to understand how microbial research topics are inevitably affected by societal influences, including (health) politics, economics and the arts. Fifty Percent Human is a multidisciplinary artistic research project that aims to reshape our understanding of the human body and its environment as well as to explore possibilities for conscious coexistence in order to bridge the gap between science and society.
DOI : 10.1111/1751-7915.13285
ISSN: 1751-7915
Sayı: 4
Cilt: 11
Sayfa: 571-574
25
Influence of the propagation strategy for obtaining robustSaccharomyces cerevisiaecells that efficiently co-ferment xylose and glucose in lignocellulosic hydrolysates
Dergi Makalesi

Dergi Makalesi
Kaynak :
Wiley Online Library
Dergi:
Microbial Biotechnology
Yazar(lar) :
Elia Tomás-Pejó , Lisbeth Olsson
Yıl :
2015
Microbial Biotechnology
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Development of xylose‐fermenting yeast strains that are tolerant to the inhibitors present in lignocellulosic hydrolysates is crucial to achieve efficient bioethanol production processes. In this study, the importance of the propagation strategy for obtaining robust cells was studied. Addition of hydrolysate during propagation of the cells adapted them to the inhibitors, resulting in more tolerant cells with shorter lag phases and higher specific growth rates in minimal medium containing acetic acid and vanillin than unadapted cells. Addition of hydrolysate during propagation also resulted in cells with better fermentation capabilities. Cells propagated without hydrolysate were unable to consume xylose in wheat straw hydrolysate fermentations, whereas 40.3% and 97.7% of the xylose was consumed when 12% and 23% (v/v) hydrolysate, respectively, was added during propagation. Quantitative polymerase chain reaction revealed changes in gene expression, depending on the concentration of hydrolysate added during propagation. This study highlights the importance of using an appropriate propagation strategy for the optimum performance of yeast in fermentation of lignocellulosic hydrolysates.
DOI : 10.1111/1751-7915.12280
ISSN: 1751-7915
Sayı: 6
Cilt: 8
Sayfa: 999-1005
26
Production of lactic acid using a new homofermentative E nterococcus faecalis isolate
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Dergi Makalesi
Kaynak :
Wiley Online Library
Dergi:
Microbial Biotechnology
Yazar(lar) :
Mohan Raj Subramanian , Suvarna Talluri , Lew P. Christopher
Yıl :
2014
Microbial Biotechnology
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Lactic acid is an intermediate‐volume specialty chemical for a wide range of food and industrial applications such as pharmaceuticals, cosmetics and chemical syntheses. Although lactic acid production has been well documented, improved production parameters that lead to reduced production costs are always of interest in industrial developments. In this study, we describe the production of lactic acid at high concentration, yield and volumetric productivity utilizing a novel homofermentative, facultative anaerobe Enterococcus faecalis CBRD01. The highest concentration of 182 g lactic acid l−1 was achieved after 38 h of fed‐batch fermentation on glucose. The bacterial isolate utilized only 2–13% of carbon for its growth and energy metabolism, while 87–98% of carbon was converted to lactic acid at an overall volumetric productivity of 5 g l−1 h−1. At 13 h of fermentation, the volumetric productivity of lactate production reached 10.3 g l−1 h−1, which is the highest ever reported for microbial production of lactic acid. The lactic acid produced was of high purity as formation of other metabolites was less than 0.1%. The present investigation demonstrates a new opportunity for enhanced production of lactic acid with potential for reduced purification costs.
DOI : 10.1111/1751-7915.12133
ISSN: 1751-7915
Sayı: 2
Cilt: 8
Sayfa: 221-229
27
Enhancement of methane production from 1-hexadecene by additional electron donors
Dergi Makalesi

Dergi Makalesi
Kaynak :
Wiley Online Library
Dergi:
Microbial Biotechnology
Yazar(lar) :
A. M. S. Paulo , A. F. Salvador , J. I. Alves , R. Castro , A. A. M. Langenhoff , A. J. M. Stams , A. J. Cavaleiro
Yıl :
2017
Microbial Biotechnology
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1‐Hexadecene‐contaminated wastewater is produced in oil refineries and can be treated in methanogenic bioreactors, although generally at low conversion rates. In this study, a microbial culture able to degrade 1‐hexadecene was enriched, and different stimulation strategies were tested for enhancing 1‐hexadecene conversion to methane. Seven and three times faster methane production was obtained in cultures stimulated with yeast extract or lactate, respectively, while cultures amended with crotonate lost the ability to degrade 1‐hexadecene. Methane production from 1‐hexadecene was not enhanced by the addition of extra hydrogenotrophic methanogens. Bacteria closely related to Syntrophus and Smithella were detected in 1‐hexadecene‐degrading cultures, but not in the ones amended with crotonate, which suggests the involvement of these bacteria in 1‐hexadecene degradation. Genes coding for alkylsuccinate synthase alpha‐subunit were detected in cultures degrading 1‐hexadecene, indicating that hydrocarbon activation may occur by fumarate addition. These findings are novel and show that methane production from 1‐hexadecene is improved by the addition of yeast extract or lactate. These extra electron donors may be considered as a potential bioremediation strategy of oil‐contaminated sites with bioenergy generation through methane production.
DOI : 10.1111/1751-7915.12886
ISSN: 1751-7915
Sayı: 4
Cilt: 11
Sayfa: 657-666
28
A modular Golden Gate toolkit for Yarrowia lipolytica synthetic biology
Dergi Makalesi

Dergi Makalesi
Kaynak :
Wiley Online Library
Dergi:
Microbial Biotechnology
Yazar(lar) :
Macarena Larroude , Youngu2010Kyoung Park , Paul Soudier , Monika Kubiak , Jeanu2010Marc Nicaud , Tristan Rossignol
Yıl :
2019
Microbial Biotechnology
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The oleaginous yeast Yarrowia lipolytica is an established host for the bio‐based production of valuable compounds and an organism for which many genetic tools have been developed. However, to properly engineer Y. lipolytica and take full advantage of its potential, we need efficient, versatile, standardized and modular cloning tools. Here, we present a new modular Golden Gate toolkit for the one‐step assembly of three transcription units that includes a selective marker and sequences for genome integration. Perfectly suited to a combinatorial approach, it contains nine different validated promoters, including inducible promoters, which allows expression to be fine‐tuned. Moreover, this toolbox incorporates six different markers (three auxotrophic markers, two antibiotic‐resistance markers and one metabolic marker), which allows the fast sequential construction and transformation of multiple elements. In total, the toolbox contains 64 bricks, and it has been validated and characterized using three different fluorescent reporter proteins. Additionally, it was successfully used to assemble and integrate a three‐gene pathway allowing xylose utilization by Y. lipolytica. This toolbox provides a powerful new tool for rapidly engineering Y. lipolytica strains and is available to the community through Addgene.
DOI : 10.1111/1751-7915.13427
ISSN: 1751-7915
Sayı: 6
Cilt: 12
Sayfa: 1249-1259
29
Bacterial decolorization of textile dyes is an extracellular process requiring a multicomponent electron transfer pathway
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Dergi Makalesi
Kaynak :
Wiley Online Library
Dergi:
Microbial Biotechnology
Yazar(lar) :
Ann Brigé , Bart Motte , Jimmy Borloo , Géraldine Buysschaert , Bart Devreese , Jozef J. Van Beeumen
Yıl :
2007
Microbial Biotechnology
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Many studies have reported microorganisms as efficient biocatalysts for colour removal of dye‐containing industrial wastewaters. We present the first comprehensive study to identify all molecular components involved in decolorization by bacterial cells. Mutants from the model organism Shewanella oneidensis MR‐1, generated by random transposon and targeted insertional mutagenesis, were screened for defects in decolorization of an oxazine and diazo dye. We demonstrate that decolorization is an extracellular reduction process requiring a multicomponent electron transfer pathway that consists of cytoplasmic membrane, periplasmic and outer membrane components. The presence of melanin, a redox‐active molecule excreted by S. oneidensis, was shown to enhance the dye reduction rates. Menaquinones and the cytochrome CymA are the crucial cytoplasmic membrane components of the pathway, which then branches off via a network of periplasmic cytochromes to three outer membrane cytochromes. The key proteins of this network are MtrA and OmcB in the periplasm and outer membrane respectively. A model of the complete dye reduction pathway is proposed in which the dye molecules are reduced by the outer membrane cytochromes either directly or indirectly via melanin.
DOI : 10.1111/j.1751-7915.2007.00005.x
ISSN: 1751-7915
Sayı: 1
Cilt: 1
Sayfa: 40-52

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