Piri Keşif Aracı

Özet Ek Bilgiler

This article is from Microbial Biotechnology, volume 6. Abstract Cultivable bacterial strains associated with field-grown Brassica napus L. (soil, rhizosphere and roots) from a trace elements (Cd, Zn and Pb) contaminated field and a non-contaminated control field were characterized genotypically and phenotypically. Correspondence analysis of the genotypic data revealed a correlation between soil and rhizosphere communities isolated from the same field, indicating that local conditions play a more important role in influencing the composition of (rhizosphere) soil bacterial communities than root exudates. In contrast, endophytic communities of roots showed a correlation between fields, suggesting that plants on the two fields contain similar obligate endophytes derived from a common seed endophytic community and/or can select bacteria from the rhizosphere. The latter seemed not very likely since, despite the presence of several potential endophytic taxa in the rhizosphere, no significant correlation was found between root and rhizosphere communities. The majority of Cd/Zn tolerant strains capable of phosphorus solubilization, nitrogen fixation, indole-3-acetic acid production and showing 1-aminocyclopropane-1-carboxylate deaminase capacity were found in the rhizosphere and roots of plants growing on the contaminated field.

DOI : doi:10.1111/1751-7915.12057

Cilt :6
ISSN :1751-7915

Sınıflandırma :

Yaşam Bilimleri %100

Mikrobiyoloji %22

Bitki Fizyolojisi %22

Bitki patolojisi %20

2

Polyphenols from olive mill waste affect biofilm formation and motility in Escherichia coli K-12.

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Internet Archive Books

Dergi:

Microbial Biotechnology

Yayıncı:

Microbial Biotechnology

Yazar(lar) :

Barbara Cardazzo , Enrico Novelli , Maurizio Servili , Stefania Balzan , Maria Elena Martino , Filomena Montemurro , Luca Fasolato , Lisa Carraro

Yıl :

2014

Microbial Biotechnology

Özet Ek Bilgiler

This article is from Microbial Biotechnology, volume 7. Abstract Olive mill wastes are sources of phenolic compounds with a wide array of biological activities, including antimicrobial effects. A potential option for bioremediation to overcome ecological problems is the reutilization of these natural compounds in food production. The aim of this work was to gain a better understanding of the antimicrobial mode of action of a phenols extract from olive vegetation water (PEOVW) at molecular level by studying Escherichia coli as a model microorganism. Genome-wide transcriptional analysis was performed on E. coli K-12 exposed to PEOVW. The repression of genes for flagellar synthesis and the involvement of genes linked to biofilm formation and stress response were observed. Sub-inhibitory concentrations of PEOVW significantly decreased biofilm formation, swarming and swimming motility, thus confirming the gene expression data. This study provides interesting insights on the molecular action of PEOVW on E. coli K-12. Given these anti-biofilm properties and considering that biofilm formation is a serious problem for the food industry and human health, PEOVW has proved to be a high-value natural product.Olive mill wastes are sources of phenolic compounds with a wide array of biological activities, including antimicrobial effects. Genome-wide transcriptional analysis was performed on E. coli K-12 exposed to phenols extract from olive vegetation water (PEOVW). Sub-inhibitory concentrations of PEOVW significantly decreased biofilm formation, swarming and swimming motility. Given these anti-biofilm properties PEOVW has proved to be a high-value natural product.

DOI : doi:10.1111/1751-7915.12119

Cilt :7
ISSN :1751-7915

Sınıflandırma :

Yaşam Bilimleri %94

Ökaryotik Mikrobiyoloji %54

Mikrobiyoloji %22

3

Initiation of the flexirubin biosynthesis in Chitinophaga pinensis.

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Internet Archive Books

Kaynak 2:

Internet Archive Books

Dergi:

Microbial Biotechnology

Yayıncı:

Microbial Biotechnology

Yazar(lar) :

Helge B Bode , Christian Schonau , Sebastian W Fuchs , Tim A Schoner

Yıl :

2014

Microbial Biotechnology

Yayıncı Web Siteleri

Özet Ek Bilgiler

This article is from Microbial Biotechnology, volume 7. Abstract Bacteria from the Bacteroidetes phylum are known producers of the chemotaxonomic relevant flexirubins. These orange pigments comprise a non-isoprenoid aryl-polyene carboxylic acid esterified with a dialkylresorcinol. Herein, we report a gene cluster from Chitinophaga pinensis encoding the biosynthesis of the polyene moiety and the biochemical characterization of a tyrosine ammonia-lyase and a 4-coumarate-CoA ligase responsible for the initiation of the polyene biosynthesis. Additionally, the flexirubin of C. pinensis was characterized by a combination of feeding experiments, high-performance liquid chromatography tandem mass spectrometry and matrix-assisted laser desorption/ionization mass spectrometry.

Kaynak 1: https://archive.org/details/pubmed-PMC3992019 arayıcılığı görüntüle.
Kaynak 2: https://archive.org/details/pubmed-PMC3992019 arayıcılığı görüntüle.

DOI : doi:10.1111/1751-7915.12110

Cilt :7
ISSN :1751-7915

Sınıflandırma :

Kimya %99

Analitik Kimya %74

4

Humic substance-mediated reduction of iron(III) oxides and degradation of 2,4-D by an alkaliphilic bacterium, Corynebacterium humireducens MFC-5.

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Dergi:

Microbial Biotechnology

Yayıncı:

Microbial Biotechnology

Yazar(lar) :

Fang-bai Li , Tian Yuan , Yong Yuan , Shun-gui Zhou , Li Zhuang , Chun-yuan Wu

Yıl :

2012

Microbial Biotechnology

Özet Ek Bilgiler

This article is from Microbial Biotechnology, volume 6. Abstract With the use of an alkaliphilic bacterium, Corynebacterium humireducens MFC-5, this study investigated the reduction of goethite (α-FeOOH) and degradation of 2,4-dichlorophenoxyacetic acid (2,4-D) mediated by different humic substances (humics) and quinones in alkaline conditions (pH of 9.0). The results indicated that (i) using sucrose as the electron donor, the strain MFC-5 was capable of reducing anthraquinone-2,6-disulfonic acid (AQDS), anthraquinone-2-disulfonic acid (AQS), anthraquinone-2-carboxylic acid (AQC), humic acid (HA) and fulvic acid (FA), and its reducing capability ranked as AQC > AQS > AQDS > FA > HA; (ii) the anaerobic reduction of α-FeOOH and 2,4-D by the strain was insignificant, while the reductions were greatly enhanced by the addition of quinones/humics serving as redox mediators; (iii) the Fe(III) reduction rate was positively related to the content of quinone functional groups and the electron-accepting capacities (EAC) of quinones/humics based on fourier-transform infrared spectroscopy (FT-IR) and electrochemical analyses; however, such a relationship was not found in 2,4-D degradation probably because quinone reduction was not the rate-limiting step of quinone-mediated reduction of 2,4-D. Using the example of α-FeOOH and 2,4-D, this study well demonstrated the important role of humics reduction on the Fe(III)/Fe(II) biogeochemical cycle and chlorinated organic compounds degradation in alkaline reducing environments.Funding Information This study was supported by the National Natural Science Foundation of China (Nos 41101211, 31070460, 41101477), and The Project Sponsored by the Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry.

DOI : doi:10.1111/1751-7915.12003

Cilt :6
ISSN :1751-7915

Sınıflandırma :

Kimya %73

Nükleer Kimya %23

Çevre %25

5

Biofilm development during the start-up period of anaerobic biofilm reactors: the biofilm Archaea community is highly dependent on the support material.

e-Kitap

Kaynak :

Internet Archive Books

Dergi:

Microbial Biotechnology

Yayıncı:

Microbial Biotechnology

Yazar(lar) :

Nicolas Bernet , Jean-P Steyer , Gaelle Santa-Catalina , Jerome Hamelin , Frederic Habouzit

Yıl :

2014

Microbial Biotechnology

Özet Ek Bilgiler

This article is from Microbial Biotechnology, volume 7. Abstract To evaluate the impact of the nature of the support material on its colonization by a methanogenic consortium, four substrata made of different materials: polyvinyl chloride, 2 polyethylene and polypropylene were tested during the start-up of lab-scale fixed-film reactors. The reactor performances were evaluated and compared together with the analysis of the biofilms. Biofilm growth was quantified and the structure of bacterial and archaeal communities were characterized by molecular fingerprinting profiles (capillary electrophoresis-single strand conformation polymorphism). The composition of the inoculum was shown to have a major impact on the bacterial composition of the biofilm, whatever the nature of the support material or the organic loading rate applied to the reactors during the start-up period. In contrast, the biofilm archaeal populations were independent of the inoculum used but highly dependent on the support material. Supports favouring Archaea colonization, the limiting factor in the overall process, should be preferred.

DOI : doi:10.1111/1751-7915.12115

Cilt :7
ISSN :1751-7915

Sınıflandırma :

Yaşam Bilimleri %48

Kimya %30

Biyomedikal Mühendisliği ve Biyoteknoloji %67

6

Discovering new bioactive molecules from microbial sources.

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Kaynak :

Internet Archive Books

Dergi:

Microbial Biotechnology

Yayıncı:

Microbial Biotechnology

Yazar(lar) :

Stefano Donadio , Margherita Sosio , Sonia Maffioli , Marianna Iorio , Paolo Monciardini

Yıl :

2014

Microbial Biotechnology

Özet Ek Bilgiler

This article is from Microbial Biotechnology, volume 7. Abstract There is an increased need for new drug leads to treat diseases in humans, animals and plants. A dramatic example is represented by the need for novel and more effective antibiotics to combat multidrug-resistant microbial pathogens. Natural products represent a major source of approved drugs and still play an important role in supplying chemical diversity, despite a decreased interest by large pharmaceutical companies. Novel approaches must be implemented to decrease the chances of rediscovering the tens of thousands of known natural products. In this review, we present an overview of natural product screening, focusing particularly on microbial products. Different approaches can be implemented to increase the probability of finding new bioactive molecules. We thus present the rationale and selected examples of the use of hypersensitive assays; of accessing unexplored microorganisms, including the metagenome; and of genome mining. We then focus our attention on the technology platform that we are currently using, consisting of approximately 70 000 microbial strains, mostly actinomycetes and filamentous fungi, and discuss about high-quality screening in the search for bioactive molecules. Finally, two case studies are discussed, including the spark that arose interest in the compound: in the case of orthoformimycin, the novel mechanism of action predicted a novel structural class; in the case of NAI-112, structural similarity pointed out to a possible in vivo activity. Both predictions were then experimentally confirmed.

DOI : doi:10.1111/1751-7915.12123

Cilt :7
ISSN :1751-7915

Sınıflandırma :

Yaşam Bilimleri %52

Mikoloji %69

Biyotıp %23

İlaç Direnci %55

Gıda Mikrobiyolojisi %29

7

Evolution of Bacillus thuringiensis Cry toxins insecticidal activity.

e-Kitap

Kaynak :

Internet Archive Books

Dergi:

Microbial biotechnology

Yayıncı:

Microbial biotechnology

Yazar(lar) :

Mario Soberon , Liliana Pardo , Claudia Rodriguez-Almazan , Blanca Ines Garcia-Gomez , Helena Porta , Isabel Gomez , Alejandra Bravo

Yıl :

2012

Microbial biotechnology

Özet Ek Bilgiler

This article is from Microbial biotechnology, volume 6. Abstract Insecticidal Cry proteins produced by Bacillus thuringiensis are use worldwide in transgenic crops for efficient pest control. Among the family of Cry toxins, the three domain Cry family is the better characterized regarding their natural evolution leading to a large number of Cry proteins with similar structure, mode of action but different insect specificity. Also, this group is the better characterized regarding the study of their mode of action and the molecular basis of insect specificity. In this review we discuss how Cry toxins have evolved insect specificity in nature and analyse several cases of improvement of Cry toxin action by genetic engineering, some of these examples are currently used in transgenic crops. We believe that the success in the improvement of insecticidal activity by genetic evolution of Cry toxins will depend on the knowledge of the rate-limiting steps of Cry toxicity in different insect pests, the mapping of the specificity binding regions in the Cry toxins, as well as the improvement of mutagenesis strategies and selection procedures.

DOI : doi:10.1111/j.1751-7915.2012.00342.x

Cilt :6
ISSN :1751-7915

Sınıflandırma :

Yaşam Bilimleri %100

Bitki patolojisi %94

8

A new species of Burkholderia isolated from sugarcane roots promotes plant growth.

e-Kitap

Kaynak :

Internet Archive Books

Dergi:

Microbial Biotechnology

Yayıncı:

Microbial Biotechnology

Yazar(lar) :

Philip Hugenholtz , Susanne Schmidt , Mark A Ragan , Phaik-Eem Lim , Cheong Xin Chan , Prakash Lakshmanan , Richard I Webb , Yun Kit Yeoh , Thierry G A Lonhienne , Chanyarat Paungfoo-Lonhienne

Yıl :

2013

Microbial Biotechnology

Özet Ek Bilgiler

This article is from Microbial Biotechnology, volume 7. Abstract Sugarcane is a globally important food, biofuel and biomaterials crop. High nitrogen (N) fertilizer rates aimed at increasing yield often result in environmental damage because of excess and inefficient application. Inoculation with diazotrophic bacteria is an attractive option for reducing N fertilizer needs. However, the efficacy of bacterial inoculants is variable, and their effective formulation remains a knowledge frontier. Here, we take a new approach to investigating diazotrophic bacteria associated with roots using culture-independent microbial community profiling of a commercial sugarcane variety (Q208A) in a field setting. We first identified bacteria that were markedly enriched in the rhizosphere to guide isolation and then tested putative diazotrophs for the ability to colonize axenic sugarcane plantlets (Q208A) and promote growth in suboptimal N supply. One isolate readily colonized roots, fixed N2 and stimulated growth of plantlets, and was classified as a new species, Burkholderia australis sp. nov. Draft genome sequencing of the isolate confirmed the presence of nitrogen fixation. We propose that culture-independent identification and isolation of bacteria that are enriched in rhizosphere and roots, followed by systematic testing and confirming their growth-promoting capacity, is a necessary step towards designing effective microbial inoculants.

DOI : doi:10.1111/1751-7915.12105

Cilt :7
ISSN :1751-7915

Sınıflandırma :

Yaşam Bilimleri %100

Mikrobiyoloji %65

9

Coverage of diarrhoea-associated Escherichia coli isolates from different origins with two types of phage cocktails.

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Kaynak :

Internet Archive Books

Dergi:

Microbial Biotechnology

Yayıncı:

Microbial Biotechnology

Yazar(lar) :

Harald Brussow , Gloria Reuteler , Kaisar A Talukder , Alejandro Cravioto , Shamima Sultana , Firdausi Qadri , Anne-C Pittet , Shafiqul A Sarker , Armando Navarro , Gilles Bourdin

Yıl :

2014

Microbial Biotechnology

Özet Ek Bilgiler

This article is from Microbial Biotechnology, volume 7. Abstract Eighty-nine T4-like phages from our phage collection were tested against four collections of childhood diarrhoea-associated Escherichia coli isolates representing different geographical origins (Mexico versus Bangladesh), serotypes (69 O, 27 H serotypes), pathotypes (ETEC, EPEC, EIEC, EAEC, VTEC, Shigella), epidemiological settings (community and hospitalized diarrhoea) and years of isolation. With a cocktail consisting of 3 to 14 T4-like phages, we achieved 54% to 69% coverage against predominantly EPEC isolates from Mexico, 30% to 53% against mostly ETEC isolates from a prospective survey in Bangladesh, 24% to 61% against a mixture of pathotypes isolated from hospitalized children in Bangladesh, and 60% coverage against Shigella isolates. In comparison a commercial Russian phage cocktail containing a complex mixture of many different genera of coliphages showed 19%, 33%, 50% and 90% coverage, respectively, against the four above-mentioned collections. Few O serotype-specific phages and no broad-host range phages were detected in our T4-like phage collection. Interference phenomena between the phage isolates were observed when constituting larger phage cocktails. Since the coverage of a given T4-like phage cocktail differed with geographical area and epidemiological setting, a phage composition adapted to a local situation is needed for phage therapy approaches against E. coli pathogens.

DOI : doi:10.1111/1751-7915.12113

Cilt :7
ISSN :1751-7915

Sınıflandırma :

Yaşam Bilimleri %99

Tıbbi Mikrobiyoloji %81

10

Cloning of ?-poly-L-lysine (?-PL) synthetase gene from a newly isolated ?-PL-producing Streptomyces albulus NK660 and its heterologous expression in Streptomyces lividans.

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Kaynak :

Internet Archive Books

Dergi:

Microbial Biotechnology

Yayıncı:

Microbial Biotechnology

Yazar(lar) :

Shufang Wang , Cunjiang Song , Xiaomeng Wang , Wenbin Guo , Ruihua Liu , Yanyan Gu , Chao Yang , Weitao Geng

Yıl :

2014

Microbial Biotechnology

Özet Ek Bilgiler

This article is from Microbial Biotechnology, volume 7. Abstract ε-Poly-L-lysine (ε-PL), showing a wide range of antimicrobial activity, is now industrially produced as a food additive by a fermentation process. A new strain capable of producing ε-PL was isolated from a soil sample collected from Gutian, Fujian Province, China. Based on its morphological and biochemical features and phylogenetic similarity with 16S rRNA gene, the strain was identified as Streptomyces albulus and named NK660. The yield of ε-PL in 30 l fed-batch fermentation with pH control was 4.2 g l−1 when using glycerol as the carbon source. The structure of ε-PL was determined by nuclear magnetic resonance (NMR) and matrix-assisted laser desorption/ionization–time of flight mass spectrometry (MALDI-TOF MS). Previous studies have shown that the antimicrobial activity of ε-PL is dependent on its molecular size. In this study, the polymerization degree of the ε-PL produced by strain NK660 ranged from 19 to 33 L-lysine monomers, with the main component consisting of 24–30 L-lysine monomers, which implied that the ε-PL might have higher antimicrobial activity. Furthermore, the ε-PL synthetase gene (pls) was cloned from strain NK660 by genome walking. The pls gene with its native promoter was heterologously expressed in Streptomyces lividans ZX7, and the recombinant strain was capable of synthesizing ε-PL. Here, we demonstrated for the first time heterologous expression of the pls gene in S. lividans. The heterologous expression of pls gene in S. lividans will open new avenues for elucidating the molecular mechanisms of ε-PL synthesis.

DOI : doi:10.1111/1751-7915.12108

Cilt :7
ISSN :1751-7915

Sınıflandırma :

Yaşam Bilimleri %99

Mikrobiyoloji %63

Uygulamalı Mikrobiyoloji %32

11

Biochemical studies on a versatile esterase that is most catalytically active with polyaromatic esters.

e-Kitap

Kaynak :

Internet Archive Books

Dergi:

Microbial Biotechnology

Yayıncı:

Microbial Biotechnology

Yazar(lar) :

Manuel Ferrer , Jesus Sanchez , Ana Isabel Pelaez , Maria-Eugenia Guazzaroni , Dolores Reyes-Duarte , Rafael Bargiela , Carlina Pena-Garcia , Ivan Lores , Monica Martinez-Martinez

Yıl :

2014

Microbial Biotechnology

Özet Ek Bilgiler

This article is from Microbial Biotechnology, volume 7. Abstract Herein, we applied a community genomic approach using a naphthalene-enriched community (CN1) to isolate a versatile esterase (CN1E1) from the α/β-hydrolase family. The protein shares low-to-medium identity (≤ 57%) with known esterase/lipase-like proteins. The enzyme is most active at 25–30°C and pH 8.5; it retains approximately 55% of its activity at 4°C and less than 8% at ≥ 55°C, which indicates that it is a cold-adapted enzyme. CN1E1 has a distinct substrate preference compared with other α/β-hydrolases because it is catalytically most active for hydrolysing polyaromatic hydrocarbon (phenanthrene, anthracene, naphthalene, benzoyl, protocatechuate and phthalate) esters (7200–21 000 units g−1 protein at 40°C and pH 8.0). The enzyme also accepts 44 structurally different common esters with different levels of enantio-selectivity (1.0–55 000 units g−1 protein), including (±)-menthyl-acetate, (±)-neomenthyl acetate, (±)-pantolactone, (±)-methyl-mandelate, (±)-methyl-lactate and (±)-glycidyl 4-nitrobenzoate (in that order). The results provide the first biochemical evidence suggesting that such broad-spectrum esterases may be an ecological advantage for bacteria that mineralize recalcitrant pollutants (including oil refinery products, plasticizers and pesticides) as carbon sources under pollution pressure. They also offer a new tool for the stereo-assembly (i.e. through ester bonds) of multi-aromatic molecules with benzene rings that are useful for biology, chemistry and materials sciences for cases in which enzyme methods are not yet available.

DOI : doi:10.1111/1751-7915.12107

Cilt :7
ISSN :1751-7915

Sınıflandırma :

Kimya %90

12

Bactericidal and bacteriostatic antibiotics and the Fenton reaction.

e-Kitap

Kaynak :

Internet Archive Books

Dergi:

Microbial Biotechnology

Yayıncı:

Microbial Biotechnology

Yazar(lar) :

Juan L Ramos , Carlos Molina-Santiago

Yıl :

2014

Microbial Biotechnology

Özet Ek Bilgiler

This article is from Microbial Biotechnology, volume 7. Abstract None

DOI : doi:10.1111/1751-7915.12120

Cilt :7
ISSN :1751-7915

Sınıflandırma :

Çevre %72

Çevre Kimyası %81

Kimya %25

Kataliz %100

13

Biochemical studies of the multicopper oxidase (small laccase) from Streptomyces coelicolor using bioactive phytochemicals and site-directed mutagenesis.

e-Kitap

Kaynak :

Internet Archive Books

Dergi:

Microbial Biotechnology

Yayıncı:

Microbial Biotechnology

Yazar(lar) :

Emma R Master , Alexander F Yakunin , Mamdouh Abou-Zaid , Greg Brown , Robert Flick , Valentina Mavisakalyan , Bihter Korbeci , Debbie Waung , Mohammed Sherif

Yıl :

2013

Microbial Biotechnology

Özet Ek Bilgiler

This article is from Microbial Biotechnology, volume 6. Abstract Multicopper oxidases can act on a broad spectrum of phenolic and non-phenolic compounds. These enzymes include laccases, which are widely distributed in plants and fungi, and were more recently identified in bacteria. Here, we present the results of biochemical and mutational studies of small laccase (SLAC), a multicopper oxidase from Streptomyces coelicolor (SCO6712). In addition to typical laccase substrates, SLAC was tested using phenolic compounds that exhibit antioxidant activity. SLAC showed oxidase activity against 12 of 23 substrates tested, including caffeic acid, ferulic acid, resveratrol, quercetin, morin, kaempferol and myricetin. The kinetic parameters of SLAC were determined for 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulphonic acid), 2,6-dimethoxyphenol, quercetin, morin and myricetin, and maximum reaction rates were observed with myricetin, where kcat and Km values at 60°C were 8.1 (± 0.8) s−1 and 0.9 (± 0.3) mM respectively. SLAC had a broad pH optimum for activity (between pH 4 and 8) and temperature optimum at 60–70°C. It demonstrated remarkable thermostability with a half-life of over 10 h at 80°C and over 7 h at 90°C. Site-directed mutagenesis revealed 17 amino acid residues important for SLAC activity including the 10 His residues involved in copper coordination. Most notably, the Y229A and Y230A mutant proteins showed over 10-fold increase in activity compared with the wild-type SLAC, which was correlated to higher copper incorporation, while kinetic analyses with S929A predicts localization of this residue near the meta-position of aromatic substrates.Funding Information Funding for this research was provided by the Government of Ontario for the project ‘FFABnet: Functionalized Fibre and Biochemicals’ (ORF-RE-05-005), and the Natural Sciences and Engineering Research Council of Canada.

DOI : doi:10.1111/1751-7915.12068

Cilt :6
ISSN :1751-7915

Sınıflandırma :

Kimya %86

Biyoteknoloji %44

Yemek Bilimi %38

14

Product recovery in microbial biotechnologyAn annotated selection of World Wide Web sites relevant to the topics in microbial biotechnology.

e-Kitap

Kaynak :

Internet Archive Books

Dergi:

Microbial Biotechnology

Yayıncı:

Microbial Biotechnology

Yazar(lar) :

Lawrence P Wackett

Yıl :

2014

Microbial Biotechnology

Özet Ek Bilgiler

This article is from Microbial Biotechnology, volume 7. Abstract None

DOI : doi:10.1111/1751-7915.12122

Cilt :7
ISSN :1751-7915

Sınıflandırma :

Yaşam Bilimleri %24

Mikrobiyal Ekoloji %36

15

Bacterial diversity in the rhizosphere of maize and the surrounding carbonate-rich bulk soil.

e-Kitap

Kaynak :

Internet Archive Books

Dergi:

Microbial biotechnology

Yayıncı:

Microbial biotechnology

Yazar(lar) :

Juan L Ramos , Estrella Duque , Amalia Roca , Paloma Pizarro-Tobias , Jennifer Solano , Pieter van Dillewijn , M Antonia Molina-Henares , Adela Garcia-Salamanca

Yıl :

2012

Microbial biotechnology

Özet Ek Bilgiler

This article is from Microbial biotechnology, volume 6. Abstract Maize represents one of the main cultivar for food and energy and crop yields are influenced by soil physicochemical and climatic conditions. To study how maize plants influence soil microbes we have examined microbial communities that colonize maize plants grown in carbonate-rich soil (pH 8.5) using culture-independent, PCR-based methods. We observed a low proportion of unclassified bacteria in this soil whether it was planted or unplanted. Our results indicate that a higher complexity of the bacterial community is present in bulk soil with microbes from nine phyla, while in the rhizosphere microbes from only six phyla were found. The predominant microbes in bulk soil were bacteria of the phyla Acidobacteria, Bacteroidetes and Proteobacteria, while Gammaproteobacteria of the genera Pseudomonas and Lysobacter were the predominant in the rhizosphere. As Gammaproteobacteria respond chemotactically to exudates and are efficient in the utilization of plants exudate products, microbial communities associated to the rhizosphere seem to be plant-driven. It should be noted that Gammaproteobacteria made available inorganic nutrients to the plants favouring plant growth and then the benefit of the interaction is common.

DOI : doi:10.1111/j.1751-7915.2012.00358.x

Cilt :6
ISSN :1751-7915

Sınıflandırma :

Yaşam Bilimleri %98

Mikrobiyoloji %64

Mikrobiyal Ekoloji %25

16

Biotransformation of the high-molecular weight polycyclic aromatic hydrocarbon (PAH) benzokfluoranthene by Sphingobium sp. strain KK22 and identification of new products of non-alternant PAH biodegradation by liquid chromatography electrospray ionization tandem mass spectrometry.

e-Kitap

Kaynak :

Internet Archive Books

Dergi:

Microbial Biotechnology

Yayıncı:

Microbial Biotechnology

Yazar(lar) :

Robert A Kanaly , Yasuhiro Ozeki , Yuji Hatada , Shinro Nishi , Allyn H Maeda

Yıl :

2013

Microbial Biotechnology

Özet Ek Bilgiler

This article is from Microbial Biotechnology, volume 7. Abstract A pathway for the biotransformation of the environmental pollutant and high-molecular weight polycyclic aromatic hydrocarbon (PAH) benzo[k]fluoranthene by a soil bacterium was constructed through analyses of results from liquid chromatography negative electrospray ionization tandem mass spectrometry (LC/ESI(–)-MS/MS). Exposure of Sphingobium sp. strain KK22 to benzo[k]fluoranthene resulted in transformation to four-, three-and two-aromatic ring products. The structurally similar four-and three-ring non-alternant PAHs fluoranthene and acenaphthylene were also biotransformed by strain KK22, and LC/ESI(–)-MS/MS analyses of these products confirmed the lower biotransformation pathway proposed for benzo[k]fluoranthene. In all, seven products from benzo[k]fluoranthene and seven products from fluoranthene were revealed and included previously unreported products from both PAHs. Benzo[k]fluoranthene biotransformation proceeded through ortho-cleavage of 8,9-dihydroxy-benzo[k]fluoranthene to 8-carboxyfluoranthenyl-9-propenic acid and 9-hydroxy-fluoranthene-8-carboxylic acid, and was followed by meta-cleavage to produce 3-(2-formylacenaphthylen-1-yl)-2-hydroxy-prop-2-enoic acid. The fluoranthene pathway converged with the benzo[k]fluoranthene pathway through detection of the three-ring product, 2-formylacenaphthylene-1-carboxylic acid. Production of key downstream metabolites, 1,8-naphthalic anhydride and 1-naphthoic acid from benzo[k]fluoranthene, fluoranthene and acenaphthylene biotransformations provided evidence for a common pathway by strain KK22 for all three PAHs through acenaphthoquinone. Quantitative analysis of benzo[k]fluoranthene biotransformation by strain KK22 confirmed biodegradation. This is the first pathway proposed for the biotransformation of benzo[k]fluoranthene by a bacterium.

DOI : doi:10.1111/1751-7915.12102

Cilt :7
ISSN :1751-7915

Sınıflandırma :

Kimya %84

Kromatografi %74

Analitik Kimya %23

17

Study of the TmoS

Dergi Makalesi

Kaynak :

DSpace Makaleler

Dergi:

Microbial Biotechnology

Yayıncı:

Wiley-Blackwell

Yazar(lar) :

Hortencia Silva-Jimenez , Cristina Garcia-Fontana , Bilge Hilal Cadirci , Maria Isabel Ramos-Gonzalez , Juan Luis Ramos , Tino Krell

Yıl :

2012

Microbial Biotechnology

Özet Ek Bilgiler

The two-component system TmoS/TmoT controls the expression of the toluene-4-monooxygenase pathway in Pseudomonas mendocina RK1 via modulation of PtmoX activity. The TmoS/TmoT system belongs to the family of TodS/TodT like proteins. The sensor kinase TmoS is a 108 kDa protein composed of seven different domains. Using isothermal titration calorimetry we show that purified TmoS binds a wide range of aromatic compounds with high affinities. Tightest ligand binding was observed for toluene (KD = 150 nM), which corresponds to the highest affinity measured between an effector and a sensor kinase. Other compounds with affinities in the nanomolar range include benzene, the 3 xylene isomers, styrene, nitrobenzene or p-chlorotoluene. We demonstrate that only part of the ligands that bind to TmoS increase protein autophosphorylation in vitro and consequently pathway expression in vivo. These compounds are referred to as agonists. Other TmoS ligands, termed antagonists, failed to increase TmoS autophosphorylation, which resulted in their incapacity to stimulate gene expression in vivo. We also show that TmoS saturated with different agonists differs in their autokinase activities. The effector screening of gene expression showed that promoter activity of PtmoX and PtodX (controlled by the TodS/TodT system) is mediated by the same set of 22 compounds. The common structural feature of these compounds is the presence of a single aromatic ring. Among these ligands, toluene was the most potent inducer of both promoter activities. Information on the TmoS/TmoT and TodS/TodT system combined with a sequence analysis of family members permits to identify distinct features that define this protein family.

Cilt: 5
Sayı: 4
ISSN: 1751-7907

Sınıflandırma :

Biyotıp %82

Reseptörler %29

Farmakoloji ve Toksikoloji %26

Gen İfadesi %24

18

BeQuIK (Biosensor Engineered Quorum Induced Killing): designer bacteria for destroying recalcitrant biofilms

Dergi Makalesi

Kaynak :

Directory of Open Access Journals (DOAJ)

Dergi:

Microbial Biotechnology

Yazar(lar) :

Pinpunya Riangrungroj , Karen M. Polizzi

Yıl :

2020

Microbial Biotechnology

Piri Tam Metin Bulucu Ek Bilgiler

DOI : 10.1111/1751-7915.13465

ISSN: 1751-7915
Cilt: 13
Sayı: 2
Sayfa: 311 - 314

Sınıflandırma :

Yaşam Bilimleri %55

Mikrobiyal Ekoloji %50

Bakteriyoloji %30

Kimya %39

Biyokimya Mühendisliği %56

Biyomedikal Mühendisliği ve Biyoteknoloji %33

19

GATA‐type transcription factor MrNsdD regulates dimorphic transition, conidiation, virulence and microsclerotium formation in the entomopathogenic fungus Metarhizium rileyi

Dergi Makalesi

Kaynak :

Directory of Open Access Journals (DOAJ)

Dergi:

Microbial Biotechnology

Yazar(lar) :

Caiyan Xin , Jie Yang , Yingyu Mao , Wenbi Chen , Zhongkang Wang , Zhangyong Song

Yıl :

2020

Microbial Biotechnology

Piri Tam Metin Bulucu Özet Ek Bilgiler

Summary The GATA‐type sexual development transcription factor NsdD has been implicated in virulence, secondary metabolism and asexual development in filamentous fungi. However, little is known about its function in the yeast‐to‐hypha transition and in microsclerotium formation. In the current study, the orthologous NsdD gene MrNsdD in the entomopathogenic fungus Metarhizium rileyi was characterized. Transcriptional analysis indicated that MrNsdD was involved in yeast‐to‐hypha transition, conidiation and microsclerotium formation. After targeted deletion of MrNsdD, dimorphic transition, conidiation, fungal virulence and microsclerotium formation were all impaired. Compared with the wild‐type strain, the ΔMrNsdD mutants were hypersensitive to thermal stress. Furthermore, transcriptome sequencing analysis revealed that MrNsdD regulated a distinct signalling pathway in M. rileyi during the yeast‐to‐hypha transition or microsclerotium formation, but exhibited overlapping regulation of genes during the two distinct developmental stages. Taken together, characterization of the MrNsdD targets in this study will aid in the dissection of the molecular mechanisms of dimorphic transition and microsclerotium development.

DOI : 10.1111/1751-7915.13581

ISSN: 1751-7915
Cilt: 13
Sayı: 5
Sayfa: 1489 - 1501

Sınıflandırma :

Yaşam Bilimleri %100

Mikrobiyal Genetik ve Genomik %100

20

Akkermansia muciniphila is a promising probiotic

Dergi Makalesi

Kaynak :

Directory of Open Access Journals (DOAJ)

Dergi:

Microbial Biotechnology

Yazar(lar) :

Ting Zhang , Qianqian Li , Lei Cheng , Heena Buch , Faming Zhang

Yıl :

2019

Microbial Biotechnology

Piri Tam Metin Bulucu Özet Ek Bilgiler

Summary Akkermansia muciniphila (A. muciniphila), an intestinal symbiont colonizing in the mucosal layer, is considered to be a promising candidate as probiotics. A. muciniphila is known to have an important value in improving the host metabolic functions and immune responses. Moreover, A. muciniphila may have a value in modifying cancer treatment. However, most of the current researches focus on the correlation between A. muciniphila and diseases, and little is known about the causal relationship between them. Few intervention studies on A. muciniphila are limited to animal experiments, and limited studies have explored its safety and efficacy in humans. Therefore, a critical analysis of the current knowledge in A. muciniphila will play an important foundation for it to be defined as a new beneficial microbe. This article will review the bacteriological characteristics and safety of A. muciniphila, as well as its causal relationship with metabolic disorders, immune diseases and cancer therapy.

DOI : 10.1111/1751-7915.13410

ISSN: 1751-7915
Cilt: 12
Sayı: 6
Sayfa: 1109 - 1125

Sınıflandırma :

Biyotıp %77

Gıda Mikrobiyolojisi %97

21

Metabolic engineering of Pseudomonas putida for increased polyhydroxyalkanoate production from lignin

Dergi Makalesi

Kaynak :

Directory of Open Access Journals (DOAJ)

Dergi:

Microbial Biotechnology

Yazar(lar) :

Davinia Salvachúa , Thomas Rydzak , Raquel Auwae , Annette De Capite , Brenna A. Black , Jason T. Bouvier , Nicholas S. Cleveland , Joshua R. Elmore , Jay D. Huenemann , Rui Katahira , William E. Michener , Darren J. Peterson , Holly Rohrer , Derek R. Vardon , Gregg T. Beckham , Adam M. Guss

Yıl :

2020

Microbial Biotechnology

Piri Tam Metin Bulucu Özet Ek Bilgiler

Summary Microbial conversion offers a promising strategy for overcoming the intrinsic heterogeneity of the plant biopolymer, lignin. Soil microbes that natively harbour aromatic‐catabolic pathways are natural choices for chassis strains, and Pseudomonas putida KT2440 has emerged as a viable whole‐cell biocatalyst for funnelling lignin‐derived compounds to value‐added products, including its native carbon storage product, medium‐chain‐length polyhydroxyalkanoates (mcl‐PHA). In this work, a series of metabolic engineering targets to improve mcl‐PHA production are combined in the P. putida chromosome and evaluated in strains growing in a model aromatic compound, p‐coumaric acid, and in lignin streams. Specifically, the PHA depolymerase gene phaZ was knocked out, and the genes involved in β‐oxidation (fadBA1 and fadBA2) were deleted. Additionally, to increase carbon flux into mcl‐PHA biosynthesis, phaG, alkK, phaC1 and phaC2 were overexpressed. The best performing strain – which contains all the genetic modifications detailed above – demonstrated a 53% and 200% increase in mcl‐PHA titre (g l−1) and a 20% and 100% increase in yield (g mcl‐PHA per g cell dry weight) from p‐coumaric acid and lignin, respectively, compared with the wild type strain. Overall, these results present a promising strain to be employed in further process development for enhancing mcl‐PHA production from aromatic compounds and lignin.

DOI : 10.1111/1751-7915.13481

ISSN: 1751-7915
Cilt: 13
Sayı: 1
Sayfa: 290 - 298

Sınıflandırma :

Yaşam Bilimleri %86

Uygulamalı Mikrobiyoloji %78

22

Integrated transcriptomic and secretomic approaches reveal critical pathogenicity factors in Pseudofabraea citricarpa inciting citrus target spot

Dergi Makalesi

Kaynak :

Directory of Open Access Journals (DOAJ)

Dergi:

Microbial Biotechnology

Yazar(lar) :

Yuheng Yang , Anfei Fang , Yang Yu , Chaowei Bi , Changyong Zhou

Yıl :

2019

Microbial Biotechnology

Piri Tam Metin Bulucu Özet Ek Bilgiler

Summary Target spot is a newly emerging citrus disease caused by Pseudofabraea citricarpa. Outbreaks of this disease result in massive economic losses to citrus production. Here, an integrated study involving comparative transcriptomic and secretomic analyses was conducted to determine the critical pathogenicity factors of P. citricarpa involved in the induction of citrus target spot. A total of 701 transcripts and their cognate proteins were quantified and integrated. Among these transcripts and proteins, 99 exhibited the same expression patterns. Our quantitative integrated multi‐omic data highlight several potentially pivotal pathogenicity factors, including 16 unigenes that were annotated as plant cell‐wall‐degrading enzymes, 13 unigenes homologous to virulence factors from various fungi, and one unigene described as a small cysteine‐rich secreted protein, were screened and analysed. The screening of differentially expressed genes that encode secondary metabolism core enzymes implicated terpene metabolism in the pathogenicity of P. citricarpa. Overall, results indicated that plant cell wall degradation, plant–pathogen protein/polyribonucleotide interaction, and terpene biosynthesis have critical roles in the pathogenicity of P. citricarpa. This work demonstrated that integrated omic approaches enable the identification of pathogenicity/virulence factors and provide insights into the mechanisms underlying the pathogenicity of fungi. These insights would aid the development of effective disease management strategies.

DOI : 10.1111/1751-7915.13440

ISSN: 1751-7915
Cilt: 12
Sayı: 6
Sayfa: 1260 - 1273

Sınıflandırma :

Yaşam Bilimleri %100

Bitki patolojisi %64

Mikrobiyal Genetik ve Genomik %30

23

Ethanol production from dilute‐acid steam exploded lignocellulosic feedstocks using an isolated multistress‐tolerant Pichia kudriavzevii strain

Dergi Makalesi

Kaynak :

Directory of Open Access Journals (DOAJ)

Dergi:

Microbial Biotechnology

Yazar(lar) :

Shuou2010Fu Yuan , Giau2010Luen Guo , Wenu2010Song Hwang

Yıl :

2017

Microbial Biotechnology

Piri Tam Metin Bulucu Özet Ek Bilgiler

Summary Renewable and low‐cost lignocellulosic wastes have attractive applications in bioethanol production. The yeast Saccharomyces cerevisiae is the most widely used ethanol‐producing microbe; however, its fermentation temperature (30–35°C) is not optimum (40–50°C) for enzymatic hydrolysis in the simultaneous saccharification and fermentation (SSF) process. In this study, we successfully performed an SSF process at 42°C from a high solid loading of 20% (w/v) acid‐impregnated steam explosion (AISE)‐treated rice straw with low inhibitor concentrations (furfural 0.19 g l−1 and acetic acid 0.95 g l−1) using an isolate Pichia kudriavzevii SI, where the ethanol titre obtained (33.4 gp l−1) was nearly 39% greater than that produced by conventional S. cerevisiae BCRC20270 at 30°C (24.1 gp l−1). In addition, P. kudriavzevii SI exhibited a high conversion efficiency of > 91% from enzyme‐saccharified hydrolysates of AISE‐treated plywood chips and sugarcane bagasse, although high concentrations of furaldehydes, such as furfural 1.07–1.21 g l−1, 5‐hydroxymethyl furfural 0.20−0.72 g l−1 and acetic acid 4.80–7.65 g l−1, were present. This is the first report of ethanol fermentation by P. kudriavzevii using various acid‐treated lignocellulosic feedstocks without detoxification or added nutrients. The multistress‐tolerant strain SI has greater potential than the conventional S. cerevisiae for use in the cellulosic ethanol industry.

DOI : 10.1111/1751-7915.12712

ISSN: 1751-7915
Cilt: 10
Sayı: 6
Sayfa: 1581 - 1590

Sınıflandırma :

Enerji %96

Yenilenebilir ve Yeşil Enerji %100

24

Enhanced production of individual ganoderic acids by integrating Vitreoscilla haemoglobin expression and calcium ion induction in liquid static cultures of Ganoderma lingzhi

Dergi Makalesi

Kaynak :

Directory of Open Access Journals (DOAJ)

Dergi:

Microbial Biotechnology

Yazar(lar) :

Junu2010Wei Xu , Tongu2010Hui Yue , Xuya Yu , Peng Zhao , Tao Li , Na Li

Yıl :

2019

Microbial Biotechnology

Piri Tam Metin Bulucu Özet Ek Bilgiler

Summary Ganoderic acids produced by Ganoderma exhibit anticancer and antimetastatic activities. A novel approach by combining Vitreoscilla haemoglobin (VHb) expression and calcium ion induction was developed to enhance ganoderic acid (GA) production in liquid static cultures of G. lingzhi. The maximum contents of GA‐O, GA‐S and GA‐Me were 1451.33 ± 67.50, 1431.23 ± 79.74 and 1283.81 ± 85.13 μg per 100 mg cell weight, respectively under the integrated approach, which are the highest contents as ever reported in Ganoderma. The contents of squalene and lanosterol were increased by 2.0‐ and 3.0‐fold in this case compared with those in the control. The transcription levels of 3‐hydroxy‐3‐methylglutaryl coenzyme A reductase, farnesyl‐diphosphate synthase, squalene synthase and cytochrome P450 CYP5150L8 were upregulated by 2.56‐, 3.31‐, 2.59‐ and 6.12‐fold respectively. Additionally, the expression of VHb improved the ratio of type I to type II GA in liquid static cultivation of G. lingzhi. The transcription levels of cyp512a2, cyp512v2 and cyp512a13, candidate cytochrome P450 genes involved in oxidative modification of the lanostane skeleton in GA biosynthesis, were also increased by 2.28‐, 2.65‐ and 3.54‐fold in the VHb‐expressing strain respectively. Our results illustrated that the approach described here efficiently improved GA production in G. lingzhi fermentation.

DOI : 10.1111/1751-7915.13381

ISSN: 1751-7915
Cilt: 12
Sayı: 6
Sayfa: 1180 - 1187

Sınıflandırma :

Yaşam Bilimleri %99

Lipidoloji %51

25

Lactobacillus casei expressing Internalins A and B reduces Listeria monocytogenes interaction with Caco‐2 cells in vitro

Dergi Makalesi

Kaynak :

Directory of Open Access Journals (DOAJ)

Dergi:

Microbial Biotechnology

Yazar(lar) :

Moloko G. Mathipa , Mapitsi S. Thantsha , Arun K. Bhunia

Yıl :

2019

Microbial Biotechnology

Piri Tam Metin Bulucu Özet Ek Bilgiler

Summary Listeria monocytogenes has been implicated in a number of outbreaks including the recent largest outbreak in South Africa. Current methods for prevention of foodborne L. monocytogenes infection are inadequate, thus raising a need for an alternative strategy. Probiotic bioengineering is considered a prevailing approach to enhance the efficacy of probiotics for targeted control of pathogens. Here, the ability of Lactobacillus casei expressing the L. monocytogenes invasion proteins Internalins A and B (inlAB) to prevent infection was investigated. The inlAB operon was cloned and surface‐expressed on L. casei resulting in a recombinant strain, LbcInlAB, and subsequently, its ability to inhibit adhesion, invasion and translocation of L. monocytogenes through enterocyte‐like Caco‐2 cells was examined. Cell surface expression of InlAB on the LbcInlAB was confirmed by Western blotting and immunofluorescence staining. The LbcInlAB strain showed significantly higher (P < 0.0001) adherence, invasion and translocation of Caco‐2 cells than the wild‐type L. casei strain (LbcWT), as well as reduced L. monocytogenes adhesion, invasion and transcellular passage through the cell monolayer than LbcWT. Furthermore, pre‐exposure of Caco‐2 cells to LbcInlAB significantly reduced L. monocytogenes‐induced cell cytotoxicity and epithelial barrier dysfunction. These results suggest that InlAB‐expressing L. casei could be a potential practical approach for prevention of listeriosis.

DOI : 10.1111/1751-7915.13407

ISSN: 1751-7915
Cilt: 12
Sayı: 4
Sayfa: 715 - 729

26

Root‐tip cutting and uniconazole treatment improve the colonization rate of Tuber indicum on Pinus armandii seedlings in the greenhouse

Dergi Makalesi

Kaynak :

Directory of Open Access Journals (DOAJ)

Dergi:

Microbial Biotechnology

Yazar(lar) :

Xiaolin Li , Lei Ye , Xiaoping Zhang , Hao Tan , Qiang Li

Yıl :

2020

Microbial Biotechnology

Piri Tam Metin Bulucu Özet Ek Bilgiler

Summary The Chinese black truffle Tuber indicum is commercially valuable. The main factors influencing the success or failure of a truffle crop include the mycorrhizal colonization rate and host plant quality. The effects of a plant growth regulator (uniconazole) and plant growth management technique (root‐tip cutting) on T. indicum colonization rate and Pinus armandii seedling growth were assessed under greenhouse conditions. The results indicated that 10 mg l−1 uniconazole or the combination of 5 mg l−1 uniconazole and root‐tip cutting constitutes an effective method for ectomycorrhizal synthesis based on an overall evaluation of colonization rate, plant biomass, plant height, root weight, stem circumference and antioxidant enzyme activities (SOD and POD) of P. armandii. The abundance of Proteobacteria in the rhizosphere of colonized seedlings might serve as an indicator of stable mycorrhizal colonization. This research inspires the potential application of uniconazole and root‐tip cutting treatments for mycorrhizal synthesis and truffle cultivation.

DOI : 10.1111/1751-7915.13511

ISSN: 1751-7915
Cilt: 13
Sayı: 2
Sayfa: 535 - 547

Sınıflandırma :

Yaşam Bilimleri %100

Bitki patolojisi %100

27

Metabolic modulation of redox state confounds fish survival against Vibrio alginolyticus infection

Dergi Makalesi

Kaynak :

Directory of Open Access Journals (DOAJ)

Dergi:

Microbial Biotechnology

Yazar(lar) :

Qiu2010yang Gong , Manu2010jun Yang , Li-fen Yang , Zhuangu2010gui Chen , Ming Jiang , Bo Peng

Yıl :

2020

Microbial Biotechnology

Piri Tam Metin Bulucu Özet Ek Bilgiler

Summary Vibrio alginolyticus threatens both humans and marine animals, but hosts respond to V. alginolyticus infection is not fully understood. Here, functional metabolomics was adopted to investigate the metabolic differences between the dying and surviving zebrafish upon V. alginolyticus infection. Tryptophan was identified as the most crucial metabolite, whose abundance was decreased in the dying group but increased in the survival group as compared to control group without infection. Concurrently, the dying zebrafish displayed excessive immune response and produced higher level of reactive oxygen species (ROS). Interestingly, exogenous tryptophan reverted dying rate through metabolome re‐programming, thereby enhancing the survival from V. alginolyticus infection. It is preceded by the following mechanism: tryptophan fluxed into the glycolysis and tricarboxylic acid cycle (TCA cycle), promoted adenosine triphosphate (ATP) production and further increased the generation of NADPH. Meanwhile, tryptophan decreased NADPH oxidation. These together ameliorate ROS, key molecules in excessive immune response. This is further supported by the event that the inhibition of pyruvate metabolism and TCA cycle by inhibitors decreased D. reiro survival. Thus, our data indicate that tryptophan is a key metabolite for the host to fight against V. alginolyticus infection, representing an alternative strategy to treat bacterial infection in an antibiotic‐independent way.

DOI : 10.1111/1751-7915.13553

ISSN: 1751-7915
Cilt: 13
Sayı: 3
Sayfa: 796 - 812

Sınıflandırma :

Biyotıp %98

Nörokimya %53

Farmakoloji ve Toksikoloji %33

28

Droplet digital polymerase chain reaction for rapid broad‐spectrum detection of bloodstream infections

Dergi Makalesi

Kaynak :

Directory of Open Access Journals (DOAJ)

Dergi:

Microbial Biotechnology

Yazar(lar) :

Yannick Wouters , Daisy Dalloyaux , Anke Christenhusz , Hennie M. J. Roelofs , Heiman F. Wertheim , Chantal P. Bleekeru2010Rovers , René H. teMorsche , Geert J. A. Wanten

Yıl :

2020

Microbial Biotechnology

Piri Tam Metin Bulucu Özet Ek Bilgiler

Summary The droplet digital polymerase chain reaction (ddPCR) is a novel molecular technique that allows rapid quantification of rare target DNA sequences. Aim of this study was to explore the feasibility of the ddPCR technique to detect pathogen DNA in whole blood and to assess the diagnostic accuracy of ddPCR to detect bloodstream infections (BSIs), benchmarked against blood cultures. Broad‐range primers and probes were designed to detect bacterial 16S rRNA (and Gram stain for differentiation) and fungal 28S rRNA. To determine the detection limit of ddPCR, 10‐fold serial dilutions of E. coli and C. albicans were spiked in both PBS and whole blood. The diagnostic accuracy of ddPCR was tested in historically collected frozen blood samples from adult patients suspected of a BSI and compared with blood cultures. Analyses were independently performed by two research analysts. Outcomes included sensitivity and specificity of ddPCR. Within 4 h, blood samples were drawn, and DNA was isolated and analysed. The ddPCR detection limit was approximately 1–2 bacteria or fungi per ddPCR reaction. In total, 45 blood samples were collected from patients, of which 15 (33%) presented with positive blood cultures. The overall sensitivity of ddPCR was 80% (95% CI 52–96) and specificity 87% (95% CI 69–96). In conclusion, the ddPCR technique has considerable potential and is able to detect very low amounts of pathogen DNA in whole blood within 4 h. Currently, ddPCR has a reasonable sensitivity and specificity, but requires further optimization to make it more useful for clinical practice.

DOI : 10.1111/1751-7915.13491

ISSN: 1751-7915
Cilt: 13
Sayı: 3
Sayfa: 657 - 668

Sınıflandırma :

Biyotıp %99

Viroloji %53

29

Laccase: a multi‐purpose biocatalyst at the forefront of biotechnology

Dergi Makalesi

Kaynak :

Directory of Open Access Journals (DOAJ)

Dergi:

Microbial Biotechnology

Yazar(lar) :

Diana M. Mate , Miguel Alcalde

Yıl :

2017

Microbial Biotechnology

Piri Tam Metin Bulucu Özet Ek Bilgiler